Page 3 - Foreword; Return Authorization Number; This will aid in the prompt repair and return of the; Contact Information
Foreword This instruction manual is designed to help you gain a thorough understanding of theoperation of the equipment. Teledyne Isco recommends that you read this manualcompletely before placing the equipment in service. Although Teledyne Isco designs reliability into all equipment, there is alway...
Page 5 - General Warnings; Liquids associated with this instrument may be classified; AVERTISSEMENT; WARNING; Avoid hazardous practices! If you use this instrument in; AVERTISSEMENT; Éviter les usages périlleux! Si vous utilisez cet instrument; WARNING; If this system uses flammable organic solvents, Teledyne
BioOptix ™ 10 Safety iii BioOptix™ 10 Safety General Warnings Before installing, operating, or maintaining this equipment, it isimperative that all hazards and preventive measures are fullyunderstood. While specific hazards may vary according to locationand application, take heed in the following ge...
Page 6 - Ce système peut utiliser des dissolvants organiques; Hazard Severity Levels; This manual applies; CAUTION; Warnings identify a potentially hazardous condition, which; DANGER; DANGER – limited to the most extreme situations
BioOptix ™ 10 Safety iv AVERTISSEMENT Ce système peut utiliser des dissolvants organiques inflammables. Pour réduire le péril qui peut être causé par l'accumulation des vapeurs explosives, Teledyne Isco recommande que vous installez ce système dans un environnement bien-aéré qui est conçu pour les m...
Page 7 - Hazard Symbols; Warnings and Cautions; Ce symbole signale la présence d’un danger d’électocution.; Warnungen und Vorsichtshinweise
BioOptix ™ 10 Safety v Hazard Symbols The equipment and this manual use symbols used to warn ofhazards. The symbols are explained below. Hazard Symbols Warnings and Cautions The exclamation point within the triangle is a warning sign alerting you of important instructions in the instrument’s technic...
Page 9 - vii; Table of Contents; Section 1 Introduction
vii BioOptix™ 10 Table of Contents Section 1 Introduction 1.1 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-11.2 System Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ...
Page 10 - viii; Section 4 System Maintenance
BioOptix™ 10Table of Contents viii 3.8 Post-experiment Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11 3.8.1 Pump Flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-113.8.2 Saving Run Data . . ...
Page 11 - List of Figures; -10 Liquid connections
BioOptix™ 10 Table of Contents ix List of Figures 1-1 BioOptix 10 front panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-31-2 BioOptix 10 left-side connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-41-3 BioOpt...
Page 12 - List of Tables
BioOptix™ 10Table of Contents x List of Tables 1-1 BioOptix 10 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-21-2 BioOptix 10 Front Panel Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-31-3 BioOptix 10...
Page 14 - System Specifications; Table 1-1 lists the BioOptix 10 specifications.; Table 1-1 BioOptix 10 Specifications; II
BioOptix™ 10Section 1 Introduction 1-2 1.2 System Specifications Table 1-1 lists the BioOptix 10 specifications. Table 1-1 BioOptix 10 Specifications Dimensions a Height: 69 cmWidth: 72 cmDepth: 61 cm Weight a,b 210 kg Power Requirement a,c The power switch is the disconnect device. 100 VAC ±10%, 2....
Page 15 - Section 1 Introduction; System Controls and; Panel Controls; BioOptix 10 front panel; Table 1-2 BioOptix 10 Front Panel Controls; Item Name
BioOptix™ 10 Section 1 Introduction 1-3 1.3 System Controls and Connectors 1.3.1 BioOptix 10 Front Panel Controls Figure 1-1 BioOptix 10 front panel 1 3 4 5 2 Table 1-2 BioOptix 10 Front Panel Controls Item Name Description 1 Manual Sample Inject Port Injection port for syringe-injected liquid sampl...
Page 16 - BioOptix 10 left-side connections; Table 1-3 BioOptix 10 Left-side Connections
BioOptix™ 10Section 1 Introduction 1-4 1.3.2 BioOptix 10 Left-side Controls and Connections Figure 1-2 BioOptix 10 left-side connections 1 2 3 4 5 7 6 Table 1-3 BioOptix 10 Left-side Connections Item Name Description 1 Buffer A Inlet Ports Ports to accept Buffer A solution for each channel. 2 Buffer...
Page 17 - Connections; BioOptix 10 right-side connections; Table 1-4 BioOptix 10 Right-side Connections; Purified Sample Outlet
BioOptix™ 10 Section 1 Introduction 1-5 1.3.3 BioOptix 10 Right-side Connections Figure 1-3 BioOptix 10 right-side connections 1 Table 1-4 BioOptix 10 Right-side Connections Item Name Description 1 Purified Sample Outlet Channels 1 through 10 which connect to the Foxy 200 Fraction Collector.
Page 18 - Table 1-5 BioOptix 10 Rear Panel Controls and Connections
BioOptix™ 10Section 1 Introduction 1-6 1.3.4 BioOptix 10 Rear Panel Controls and Connections Figure 1-4 BioOptix 10 rear panel controls and connections 1 2 3 4 Table 1-5 BioOptix 10 Rear Panel Controls and Connections Item Name Description 1 Cooling Fan Ventilates the BioOptix 10 case and cools inte...
Page 19 - Section 2 Installation; The system is heavy. Use a two-person lift to prevent injury.; BioOptix 10 System; If there is any evidence that the system has been damaged; Installation; ) of bench space. Space requirements are shown in Figure
2-1 BioOptix™ 10 Section 2 Installation This section provides instructions for installing the system andpreparing it for operation. T h e B i o O p t i x 1 0 s y s t e m c o n s i s t s o f a B i o O p t i x 1 0 u n i t , aFo x y 2 0 0 F r a c t i o n C o l l e c t o r, a n d a W i n d o w s P C r u...
Page 20 - Requirements
BioOptix™ 10Section 2 Installation 2-2 To keep tubing length at a minimum, buffer, sample, and wastecollection containers should be placed to the left of the unit. Thecontainers may also be kept behind the unit as long as the con-tainers are accessible. The Foxy 200 should be installed to the right ...
Page 25 - Section 2 Installation; Figure 2-10 Liquid connections; Note
BioOptix™ 10 Section 2 Installation 2-7 10. Connect the tubing from the column module to the pump module. Refer to Figure 2-10. a. Remove the two thumbscrews securing the splash guard to the pump module. Set aside the splash guard. Note The tubing numbers from the column module match the num-bers ab...
Page 27 - Foxy 200 X-Y Fraction Collector; Figure 2-13 Foxy arm
BioOptix™ 10 Section 2 Installation 2-9 12. Ensure the Power Switch is in the Off (“0”) position. Then, connect the AC power cord to the back of the Pump Mod-ule. Connect the other end of the power cord to an outlet meeting the electrical requirements listed on the serial number label. 13. Locate th...
Page 28 - ment Foxy; Buffer A –
BioOptix™ 10Section 2 Installation 2-10 5. Connect the AC power cord to the back of the Foxy 200. Connect the other end of the power cord to an outlet meet-ing the electrical requirements listed on the serial number label. 6. Connect the “Y” Serial Cable end labeled Second Instru- ment Foxy to the R...
Page 30 - Table 2-1 Tubing Color chart; Channel Numbers; Blue
BioOptix™ 10Section 2 Installation 2-12 Buffer B – To form gradient buffers during purification, connect Buffer B solution(s) to these ten inlet ports. Perform the stepsdescribed in Buffer A above to complete these fluid connections. Sample In – T h e f e r r u l e s o n t h e s e i n l e t p o r t ...
Page 32 - Connection; Computer Serial; BioOptix 10 Software; PC Minimum Requirements
BioOptix™ 10Section 2 Installation 2-14 2.2.6 Windows PC Connection The Windows ® personal computer (PC) must meet the following minimum requirements: The PC should be set up according the manufacturer’s instruc-tions. After the PC is fully operational, place it on the workbenchwithin 2 meters of th...
Page 33 - Preparation; Power
BioOptix™ 10 Section 2 Installation 2-15 ❍ Rack Change Bell – When this option is enabled, the system provides an audible signal while it is waiting for the operator to change the tube rack. ❍ Com Port – select the Com Port number to which the “Y” Serial cable is connected to the PC. Refer to the do...
Page 37 - Section 3 Operation; Sample Preparation; Adapters; To install the column tubing:
3-1 BioOptix™ 10 Section 3 Operation This section provides an introduction to preparing the systemand creating files necessary for a purification run. The instruc-t i o n s a s s u m e t h a t t h e s y s t e m h a s b e e n s e t u p a c c o r d i n g t oSection 2. 3.1 Sample Preparation The Sample...
Page 38 - Gradient File
BioOptix™ 10Section 3 Operation 3-2 3.3 BioOptix 10 Software Note Refer to On-line Help for specific BioOptix 10 software operat-ing instructions. 3.3.1 Overview The operation of the BioOptix 10 system is directed by threefiles: • Gradient – these files define how a gradient is to be formed from Buf...
Page 39 - Section 3 Operation; Volume
BioOptix™ 10 Section 3 Operation 3-3 Note The percent B value is the amount of Buffer B solution mixedwith Buffer A. A value of zero percent B (0 %B) pumps onlyBuffer A. Conversely, a value of 100 %B pumps only Buffer B. All other rows in the table define the %B that will be reachedafter pumping the...
Page 41 - Load tab settings
BioOptix™ 10 Section 3 Operation 3-5 Pausing at the end of a block allows for buffer changes orother mechanical adjustments before proceeding with thenext block. • Wavelength – Type or select the desired wavelength from 190 to 360 nm to be used for UV detection. Note that all blocks use the same wav...
Page 43 - The Elute tab is a “Gradient” block type. See Appendix A.
BioOptix™ 10 Section 3 Operation 3-7 • Collect Effluent – Select the desired action for effluent fluid as it comes off the column. If Yes is selected, enter the desired fraction size. Note The Elute tab is a “Gradient” block type. See Appendix A. 3.5.5 Flush Settings T hi s blo ck pu m ps f luid t o...
Page 44 - Experiment; Sample Name
BioOptix™ 10Section 3 Operation 3-8 3.6.1 Setting up the Experiment Before proceeding, determine which BioOptix 10 channel posi-tions will be used during the purification run. Channels that willbe in use require that the corresponding rows in the table becompleted. Note that to use these positions t...
Page 45 - File; BioOptix 10 Operation Buttons; Table 3-1 Operation Buttons; Button
BioOptix™ 10 Section 3 Operation 3-9 3.6.3 Saving the Experiment File Save the experiment by selecting the File>Save menu command.Use a descriptive file name so that it can be located for later use. 3.7 Running the Experiment Wit h t h e e x p e r i m e n t f i l e o p e n , s o m e B i o O p t i...
Page 46 - Operation; Stop
BioOptix™ 10Section 3 Operation 3-10 Figure 3-9 Pause at end of block If the block is set to collect effluent (Figure 3-4), the fraction col-lector will deposit the fluid in the collection tubes. If a peak isdetected, the fraction collector automatically advances to thenext row to isolate the fluid....
Page 47 - Figure 3-10 Pump flush option
BioOptix™ 10 Section 3 Operation 3-11 The Fast Forward button is active after clicking the Stop button. The Fast Forward button allows you to skip to the next block in the experiment or abort the run. 3.8 Post-experiment Operation 3.8.1 Pump Flush At the completion of the experiment, the BioOptix 10...
Page 51 - Periodic Maintenance; The pump flush can also be initiated from a menu command.; menu command. This opens
4-1 BioOptix™ 10 Section 4 System Maintenance 4.1 Periodic Maintenance T h e B i o O p t i x 1 0 s y s t e m ’s i n t e r n a l c o m p o n e n t s h a v e b e e nse lecte d for lo ng life and lo w maintenance. To maintain thesystem, the only required periodic maintenance is to flush thepumps daily....
Page 52 - Exterior Cleaning; Replacement; Risk of electric shock. Disconnect power before servicing.; Earth ground bonding connector. Do not remove or
BioOptix™ 10Section 4 System Maintenance 4-2 The BioOptix 10 system pumps the wash fluid, flushing theBuffer In lines, gradient formation components, pump, and purgelines. (See Figure B-6 in Appendix B.) 4.2 Exterior Cleaning For general cleaning of the enclosure, use distilled water with amild dete...
Page 53 - Refer to Figure 4-3 for the following steps.
BioOptix™ 10 Section 4 System Maintenance 4-3 Figure 4-2 Access the pump assembly Note Refer to Figure 4-3 for the following steps. 9. Remove the bottom retainer plate. 10. On the top of the pump assembly, remove the two 10-32 × 3 / 8 " pan head screws and lock washers. 11. Lift the piston plug ...
Page 55 - Section 4 System Maintenance; shooting
BioOptix™ 10 Section 4 System Maintenance 4-5 17. Hold the push rod upside-down and insert it into the open- ing on the seal. Apply downward force until the seal snaps onto the push rod. 18. Refer to Figure 4-3 and reassemble the pump channel using new O-rings at the top and bottom of the glass cyli...
Page 56 - Remove the two screws that secure the waste port mount-
BioOptix™ 10Section 4 System Maintenance 4-6 CAUTION Hot surfaces may cause burns. The UV lamp and nearby sur-faces are hot. To remove the lamp: 1. Remove the two screws that secure the waste port mount- ing plate (Figure 4-5). The optical assembly is located behind this plate. Figure 4-5 Waste port...
Page 57 - Carefully insert the replacement bulb (part number; Table 4-1 Common Problems; Problem
BioOptix™ 10 Section 4 System Maintenance 4-7 Replacing the lamp To install the new lamp: CAUTION Contaminants on the glass bulb will shor ten the life span. Oilfrom your skin is one such contaminant. Wear clean cottongloves when handling the deuterium lamp. 1. Carefully insert the replacement bulb ...
Page 58 - Risk of electrical shock. Disconnect the electrical power
BioOptix™ 10Section 4 System Maintenance 4-8 4.5.2 Leaks If leaks occur or if solid material is observed around a fitting,determine the source of the leak and tighten the fitting. Note When you tighten a fitting, make sure that you do not over-tighten it. Overtightening a fitting may lead to permane...
Page 59 - Circuit breaker location
BioOptix™ 10 Section 4 System Maintenance 4-9 WARNING Risk of damage to eyesight and skin. Wear ultraviolet (UV) light eye protection and protect skin from exposure if the deuterium lamp is operating. CAUTION Hot surfaces may cause burns. The UV lamp and nearby sur-faces are hot. To reset the circui...
Page 60 - Service Department; If you prefer, you can send an e-mail detailing your problem to:; How to Ship Returns
BioOptix™ 10Section 4 System Maintenance 4-10 4.6 Service Department If you have a problem with the instrument or need parts infor-mation, contact Isco’s Service Department. If you write, pleaseinclude all pertinent information that may be helpful in solvingyour problem. Address your letter to: Tele...
Page 61 - Appendix A Method Templates; A.1 Opening the Template; Word-processing programs such as Microsoft Word
A-1 BioOptix™ 10 Appendix A Method Templates When creating a new method, the BioOptix 10 software con-figures the blocks of the method file according to a template file.This template file can be modified, allowing it to be customizedfor many applications. A method can have as few as one block or as ...
Page 63 - Template; Figure A-2 Customizable values in the Standard.tpl file; Numeric values
BioOptix™ 10 Appendix A Method Templates A-3 5. Check the box labeled Always use this program to open these files . 6. Click OK. After doing so, double-clicking on any file with the .tpl filename extension will open it in the selected text editor. A.2 Modifying the Template Once open in a text edito...
Page 65 - Appendix B Fluid Path Diagrams; Figure B-1 Fluid path for Fluid-type blocks
B-1 BioOptix™ 10 Appendix B Fluid Path Diagrams This appendix contains simplified diagrams of the plumbing andvalves internal to the BioOptix 10 system. Various operatingstates are depicted for a single channel. A complete plumbingdiagram can be found in Figure B-7. An understanding of fluids paths ...
Page 68 - Figure B-6 Pump flush
BioOptix™ 10Appendix B Fluid Path Diagrams B-4 B.4 Pump Flush A Pump Flush should be done whenever the system will be idlefor more than a few hours. (See sections 3.8 and 4.1.) The flushremoves salts from the Buffer in lines to the Mode knob (FigureB-6). Figure B-6 Pump flush B.5 Plumbing Diagram A ...
Page 71 - Appendix C Replacement Parts Lists
C-1 BioOptix™ 10 Appendix C Replacement Parts Lists Replacement parts are called out in illustrations in this section.Reference the call-outs in the accompanying tables to determinethe part number for the item. Replacement parts can be purchased by contacting TeledyneIsco’s Customer Service Departme...
Page 73 - C.1 Detector Assembly
13BioOptix™ 10 Replacement Parts Lists C-3 13BioOptix™ 10 Replacement Parts Lists C.1 Detector Assembly
Page 85 - CE Declar; DECLARATION OF CONFORMITY; Standard Description
Compliance Statements CE Declar ati on of Conf orm ity DECLARATION OF CONFORMITY Application of Council Directive: 89/336/EEC – The EMC Directive 73/23/EEC – The Low Voltage Directive Manufacturer's Name: Teledyne Isco, Inc. Manufacturer's Address: 4700 Superior, Lincoln, Nebraska 68504 USA Mailing ...
Page 87 - Return Authorization; Teledyne Isco One Year
Warranty In the event of instrument problems, always contact the Teledyne Isco Service Department, as problems can often be diagnosed and corrected without requiring an on-site visit. In the U.S.A., contact Teledyne Isco Service at the numbers listed below. International customers should contact the...
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