Page 3 - Leica SP2600 Ultramilling attachment; NOTE
3 Leica SP2600 Ultramilling attachment The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state- of-the-art technology as we understand it follow- ing thorough investigation in this field. We are under no obli...
Page 4 - Table of contents
4 Supplementary instruction manual V 1.2 07/2002 Table of contents 1. Important information ................................................................................................................ 6 1.1 Symbols in the text and their meaning ....................................................
Page 5 - Leica SP 2600 Ultramilling attachment; Important information
5 Leica SP 2600 Ultramilling attachment Important information 6. Troubleshooting ....................................................................................................................... 44 7. Cleaning, disinfection and maintenance .......................................................
Page 7 - Important information; Information on this instruction manual; Instrument type; only to the instrument type indicated on the title page.; Working with this instruction manual; SP2600 ultramilling attachment.; instruction manual must be supplemented by additional appropri-
7 Leica SP 2600 Ultramilling attachment 1. Important information 1.3 Information on this instruction manual Instrument type This supplementary instruction manual must be used together with the Leica SM2500 sliding microtome instruction manual describing the basic instru- ment Leica SM2500, which i...
Page 8 - Safety; Safety regulations; with the Leica SM2500 sliding microtome!
8 Supplementary instruction manual V 1.2 07/2002 2. Safety 2.1 Safety regulations This instrument has been built and tested in accordance with the same di- rectives and standards as the Leica SM2500.For a complete list of the directives and standards applied please refer to chapter 2.1, page 8 of ...
Page 9 - The instrument is equipped with the following safety devices:; Plexiglass protective cover; The miller is equipped with a protective cover made of plexiglass (; no matter whether the spindle is rotating or not.; Important note on the safety devices built in by the manufacturer:
9 Leica SP 2600 Ultramilling attachment 2. Safety 2.2 Built-in safety devices The instrument is equipped with the following safety devices: Plexiglass protective cover The miller is equipped with a protective cover made of plexiglass ( 1 ). When the cover is moved to the upper limit position ( 1...
Page 10 - Safety instructions - general; Safety instructions; Safety instructions - transport and installation
10 Supplementary instruction manual V 1.2 07/2002 Be sure to read and comply with the safety instructions, warnings and cau- tions in this chapter as well as in chapter 2 of the Leica SM2500 instruc- tion manual, even if you are already fa- miliar with the operation and use of other Leica products...
Page 13 - Ultramilling attachment; tained in the Leica SM2500 instruction manual.
13 Leica SP 2600 Ultramilling attachment 3. Technical Data Ultramilling attachment Milling spindle (special design) ......................................................................................... type TSAV 60 x 160Motor, rotation speed selectable in 100-rpm steps ...........................
Page 14 - Installation
14 Supplementary instruction manual V 1.2 07/2002 4. Installation 4.1 Overview - Leica SP2600 Ultramilling attachment / basic instrument Leica SM2500 - instrument parts / functions 1. Leica SM2500 - basic instrument (sliding microtome)1.1 Leica SP2600 - ultramilling attachment1.2 Carrier for ultra...
Page 15 - Site requirements
15 Leica SP2600 Ultramilling attachment 4. Installation 4.2 Leica SP2600 standard delivery Standard delivery includes:1 Basic instrument Leica SP2600 (Ultramilling attachment) 1 Calibrating device with dial gauge for miller adjustment 3 Holders, each with two springs, for pre- and finishing miller...
Page 16 - Installing the Leica SP2600 ultramilling attachment
4.5 Installing the Leica SP2600 ultramilling attachment Place the carrier ( 1, Fig. 4.2 ) for the ultramilling attachment ( 3, Fig. 4.3 ) on the rear part of the Leica SM2500 microtome fitting the two pins of the carrier into the corresponding bores in the Leica SM2500 microtome. Lower the beari...
Page 17 - Connecting the vacuum cleaner; Milling dust is extracted by a vacuum cleaner, through a nozzle (; Installing vacuum cleaners rated at 230 V/ 50 Hz / max. power draw 4 A; Installing other vacuum cleaners:; the rear of the ultramilling attachment.
4. Installation 4.6 Connecting the vacuum cleaner Milling dust is extracted by a vacuum cleaner, through a nozzle ( 1 ) located in the milling area. 230 V / 50 Hz Vacuum cleaners with a power draw of maximum 4 A can be connected directly to the SM2500 control unit and thus be switched on/off via the...
Page 18 - Installing additional accessories; Connect the foot switch.; The foot switch must be connected - otherwise the instrument will
18 Supplementary instruction manual V 1.2 07/2002 4.7 Installing additional accessories Install further accessories as described in chapters 4.5.2 and 4.5.3, pages 18 and 19 of the Leica SM2500 instruction manual. Connect the foot switch. The foot switch must be connected - otherwise the instr...
Page 19 - Overview of chapter 5 Operation; sure to read the following instruction manuals:; stages
19 Leica SP 2600 Ultramilling attachment 5.1 Overview of chapter 5 Operation Prior to working with the Leica SP2600 ultramilling attachment, be sure to read the following instruction manuals: The entire Leica SM2500 instruction manual except chapters 5.5.6 through 5.7. This supplementary ins...
Page 20 - calibrating device; Preparatory steps
20 Supplementary instruction manual V 1.2 07/2002 5.2 Adjusting the pre-millers in the calibrating device Preparatory steps Connect mains power supply ( 20 ) to miller cali- bration device ( 18 ). Connect mains power supply ( 20 ) to mains: the light bulb is switched on. Place magnifier ( 16...
Page 21 - Adjusting the miller; can no longer be moved.
21 Leica SP2600 Ultramilling attachment Adjusting the miller The miller ( 3 ) is adjusted in the holder ( 2 ) along three different axis (x, y and z - see Fig. 5.3 , left). Adjustment along the x-axis is done outside the calibration device ( 18, Fig. 5.1 ). For adjustment along the z- and y-axis, ...
Page 22 - Adjustment along the z-axis
22 Supplementary instruction manual V 1.2 07/2002 Adjustment along the z-axis 1. Pull lever ( 12 ) at the left of calibration device ( 18 ) forward as far as it will go, thus moving contact plate ( 17 ) backwards to its rear limit position. This prevents the contact plate from hitting the milling ...
Page 24 - Adjustment along the y-axis
24 Supplementary instruction manual V 1.2 07/2002 Adjustment along the y-axis 1. Select reference value 1. The reference value will be needed later on to adjust the finishing miller to the same milling level as the pre- miller. Any reference value can be selected. Experience, however, shows that...
Page 25 - Adjusting the finishing millers in the; Carry out preparatory steps as per chapter 5.2,; Twisted or deformed springs for the; Insert spring and finishing miller as described; When the finishing miller is inserted
25 Leica SP2600 Ultramilling attachment 5.3 Adjusting the finishing millers in the calibrating device Carry out preparatory steps as per chapter 5.2, page 20 (Adjusting the pre-millers in the cali- brating device). Twisted or deformed springs for the miller holders must be exchanged! Insert ...
Page 26 - Adjusting the finishing miller; justed in the miller holder along three different; Adjustment along the x-axis; Carry out steps 1 - 4 as described on page 22
26 Supplementary instruction manual V 1.2 07/2002 Adjusting the finishing miller Just like the pre-miller, the finishing miller is ad- justed in the miller holder along three different axis (see page 21). Adjustment along the x-axis As described for the pre-miller - see page 21. Adjustment along t...
Page 29 - Specimen holder systems - how to combine individual components
29 Leica SP 2600 Ultramilling attachment 5.4 Specimen holder systems - how to combine individual components 5. Operation Configurations: 1. Base plate with dovetail guide (0383 09422) and holder for round specimens (0383 08992) = order no. 0383 08993 2. Base plate with dovetail guide (0383 09422) ...
Page 30 - Operation
30 Supplementary instruction manual V 1.2 07/2002 5.5 Clamping specimen For a detailed overview of the clamping systems available, please refer to chapter, 5.4, page 29. 5.5.1 Installing the base plates in the basic instrument For detailed instructions on how to install the base plates in the basi...
Page 31 - the base plates; Clamping the vacuum stages in the base plate
31 Leica SP2600 Ultramilling attachment 5.5.3 Clamping the vacuum stages on/in the base plates Clamping the vacuum stages in the base plate with vise (Fig. 5.15) Fasten the vacuum stage on the vacuum stage adapter for the base plate with vise (with 4 Allen screws ( 1 ) / Allen key size 4). The 4...
Page 32 - placing specimens onto the vacuum; Connecting the vacuum pump
32 Supplementary instruction manual V 1.2 07/2002 5. Operation 5.5.4 Connecting the vacuum pump / placing specimens onto the vacuum stages IMPORTANT: Prior to first use, every vacuum table surface must be milled once, just like a specimen surface. This ensures that the surface of the specimens mil...
Page 33 - Placing specimens onto vacuum stage for 30 -
33 Leica SP2600 Ultramilling attachment Placing specimens onto vacuum stage for 30 - 50-mm lids Fasten a lid suited to the size of the specimen to be milled onto the vacuum stage (slotted screws are part of standard delivery of vacuum stage lids - use slotted screwdriver). Place the specimen (...
Page 34 - Preparatory steps prior to the milling procedure; For a detailed description of all control elements located on the; Select manual mode of operation; MAN; Move the milling spindle to the upper limit position (button (; the miller holders to the milling spindle!
34 Supplementary instruction manual V 1.2 07/2002 5.6 Preparatory steps prior to the milling procedure For a detailed description of all control elements located on the control unit, please refer to chapters 5.3 (Overview - control panel) and 5.4 (Description of each individual control element...
Page 35 - manual) or - if you need to mill a vacuum table prior to first use - see
35 Leica SP 2600 Ultramilling attachment Insert the specimen (see chapters 5.5.2 and 5.5.4, pages 30 and 33 of this manual) or - if you need to mill a vacuum table prior to first use - see chapter 5.5.3, page 31 of this manual). Wear protective gloves when working with potentially infectious spe...
Page 36 - Select automatic mode of operation ; AUTO; Select STOP or PHOTO position (buttons (; Individual parameter settings depend on specimen material and
36 Supplementary instruction manual V 1.2 07/2002 5.7 Pre-milling Select automatic mode of operation AUTO (button ( 5 ) - chapter 5.4.3, page 26 Leica SM 2500 instruction manual). In AUTO mode, set the milling parameters for the pre-milling procedure: Select STOP or PHOTO position (button...
Page 37 - Recommended milling thickness settings for pre-milling; 10 μm - keep milling until surface is completely level.; Recommended sledge speed settings for pre-milling; Return stroke: any speed; Recommended rotational speed of miller for pre-milling; Select continuous mode of operation.; Starting the milling procedure; Switch on the vacuum cleaner (via button (
37 Leica SP 2600 Ultramilling attachment Recommended milling thickness settings for pre-milling 10 µm - keep milling until surface is completely level. 5 µm - three entire sledge strokes (milling and return stroke). 3 µm - three entire sledge strokes (milling and return stroke). Do not excee...
Page 38 - Pre-milling procedure
38 Supplementary instruction manual V 1.2 07/2002 5.7.1 Perspective view of the pre-milling procedure 5. Operation Pre-milling procedure The milling spindle carrying the pre-miller rotates clockwise (circular arrow ( 1 )) while the specimen sledge passes underneath the miller in the direction of a...
Page 39 - Ending the pre-milling procedure; p. 34 - instruction manual Leica SM2500) the miller continues to
39 Leica SP 2600 Ultramilling attachment 5. Operation Ending the pre-milling procedure Press RUN STOP or RUN ENABLE to stop the sledge (buttons ( 29 ), ( 30 ) - Chapter 5.4.10, page 33 - Leica SM2500 manual) - or, when working in PROG mode of operation, wait for the programmed number of stro...
Page 40 - Finishing milling; to attaching the miller holders to the milling spindle!; men surface as described for the pre-miller on page 35.; AUTO; and set the; Recommended milling thickness settings for finishing milling; Do not exceed a milling thickness setting of 5 μm with the follow-
40 Supplementary instruction manual V 1.2 07/2002 5.8 Finishing milling Attach the miller holder containing the finishing miller to the milling spindle (Allen screws / Allen key size 5) - see Fig. 5.21, page 34). Always slide the plexiglass cover to the upper limit position prior to attaching th...
Page 41 - Recommended sledge speed settings for finishing milling; Recommended rotational speed of miller for finishing milling
41 Leica SP 2600 Ultramilling attachment Recommended sledge speed settings for finishing milling Milling stroke: 0.5 mm/s to 1.0 mm/s Return stroke: any speed Recommended rotational speed of miller for finishing milling < 2.000 rpm Recommended mode of operation for finishing milling Sel...
Page 42 - Perspective view of the finishing milling procedure; Finishing milling procedure; )) while the specimen sledge passes underneath the miller in the di-
42 Supplementary instruction manual V 1.2 07/2002 5.8.1 Perspective view of the finishing milling procedure 5. Operation Finishing milling procedure The milling spindle carrying the finishing miller rotates clockwise (circular arrow ( 1 )) while the specimen sledge passes underneath the miller in ...
Page 43 - Ending the finishing milling procedure; Switching the instrument off (switching off the control unit)
43 Leica SP 2600 Ultramilling attachment Ending the finishing milling procedure Press RUN STOP or RUN ENABLE to stop the sledge (buttons ( 29 ), ( 30 ) - chapter 5.4.10, page 33 - Leica SM2500 manual) - or, when working in PROG mode of operation, wait for the programmed number of strokes to ...
Page 44 - Troubleshooting; chapter 6, page 64 of the Leica SM2 500 instruction manual .
44 Supplementary instruction manual V 1.2 07/2002 6. Troubleshooting 6. Troubleshooting For all instructions on error detection and trouble shooting, please refer to chapter 6, page 64 of the Leica SM2 500 instruction manual .
Page 45 - Cleaning, disinfection and maintenance; Cleaning and disinfection; Cleaning; the detergents listed above.; Disinfection; pose of as per the regulations applicable.
45 Leica SP 2600 Ultramilling attachment 7. Cleaning, disinfection and maintenance 7.1 Cleaning and disinfection Only authorized service technicians may access the internal com- ponents of the instrument for service and repair! Cleaning detergents appropriate for the ultramilling attachment - varn...
Page 46 - Only authorized service technicians may access the internal com-; Carry out the maintenance steps to be done by the user as per the in-
46 Supplementary instruction manual V 1.2 07/2002 7. Cleaning, disinfection and maintenance 7.2 Maintenance Only authorized service technicians may access the internal com- ponents of the instrument for service and repair! No liquids may enter the interior of the instrument during mainte- nance! ...
Page 47 - Replacing the light bulb in the calibration device
47 Leica SP 2600 Ultramilling attachment 7.2.2 Replacing the light bulb in the calibration device Remove base cover ( 2 ) of calibration device ( 1 ) (unscrew 4 slotted screws ( 3 )). Loosen light bulb holder ( 5 ) (metal bridge with lamp socket) (unscrew 2 slotted screws ( 4 )). Take replac...
Page 48 - Miller holders and millers; Miller holder; Holder for pre- and finishing miller; Finishing miller; finishing miller; Base plates for specimen holders and vacuum stage adapters; Specimen clamp 44 x 58 mm,; Vacuum stage adapters
48 Supplementary instruction manual V 1.2 07/2002 8. Overview - accessories 8.1 Miller holders and millers Miller holder Holder for pre- and finishing miller Pre-miller Pre-miller D - with diamond edge, cutting angle -1°, clearance angle +3° Finishing miller Finishing miller D - with diamond e...
Page 49 - Vacuum stages and matching lids / perforated specimen holders; Vacuum stage for Leica SP2600 - basic body for lids 30 - 50 mm
49 Leica SP 2600 Ultramilling attachment 8.5 Vacuum stages and matching lids / perforated specimen holders Vacuum stage for Leica SP2600 - basic body for lids 30 - 50 mm Lid, 30 mm, for Leica SP2600 vacuum stage Lid, 40 mm, for Leica SP2600 vacuum stage Lid, 50 mm, for Leica SP2600 vacuum st...
Page 50 - Additional accessories; Vacuum cleaner and vacuum cleaner accessories; Vacuum cleaner Fakir S20, with connection hose; Illumination; Halogen spotlight illumination; Overview - accessories
50 Supplementary instruction manual V 1.2 07/2002 8.7 Additional accessories Vacuum cleaner and vacuum cleaner accessories Vacuum cleaner Fakir S20, with connection hose 230 V / 50 Hz only Set of vacuum cleaner bags for Fakir S 20 Illumination Halogen spotlight illumination 230 V/50 Hz onl...
Page 51 - Warranty and service
51 Leica SP 2600 Ultramilling attachment 9. Warranty and service For all information on warranty and service, please refer to chapter 9, pages 74 - 76 of the Leica SM2500 instruction manual. 9. Warranty and service