Leica VT1200S- Manual

6 Operating Manual V1.1 – 05/2007 2. Safety This Operating Manual contains important instructions and informationregarding the operational safety and maintenance of the instrument. The Operating Manual is an important part of the product, and must be readcarefully prior to startup and use and must a...

7 Leica VT1200 and VT1200 S – Microtome Caution: Extremely sharp blades pose risk of injury when touched! Fresh tissue poses risk of infection! Fire hazard from uncovered magnifier! Cover the magnifier during work breaks! 2. Safety Proper handling Always be exceptionally careful when handling the bl...

8 Operating Manual V1.1 – 05/2007 3. Instrument Characteristics 3.1 Technical Data for the VT1200 General data: Cutting frequency (± 10 %) ................................................................................................................. 85 Hz (± 10%)Amplitude ...........................

11 Leica VT1200 and VT1200 S – Microtome Specimen plate for specimens of1 cm in height for specimens of2 cm in height Directional 3. Instrument Characteristics Attachments on dovetail receptacle Ice bath Buffer tray Buffer tray,plastic Buffer tray,metal Double-walled buffer tray VibroCheck Specimen ...

14 Operating Manual V1.1 – 05/2007 4. Installation 4.1 Standard Scope of Delivery for the VT1200 VT1200 basic instrument .................................................................... 0481 42065 1 control panel ......................................................................................

15 Leica VT1200 and VT1200 S – Microtome 4.1.1 Standard Scope of Delivery for the VT1200 S VT1200 S basic instrument ................................................................. 0481 42066 1 control panel ..................................................................................... 0481...

16 Operating Manual V1.1 – 05/2007 4.2 Packing and Setting up the Instrument 4. Installation 1 . Have 2 people grab the instrument by the transport handles ( 1 ), place it on the wooden pallet and screw it to the pallet using the 4 size 6 hexa-gon-head screws ( 2 ). 2 . Pull the transparent protecti...

17 Leica VT1200 and VT1200 S – Microtome 4. Installation 4.3 Before Commissioning the Instrument 1 1 Fig. 13 Once the instrument is in its final location, unscrewthe transport handles ( 1 ) from the instrument us- ing the size 6 Allen key provided and store them,along with the screws, in a safe plac...

18 Operating Manual V1.1 – 05/2007 5. Working with the Instrument 5.1 Description of the Typical Application The Leica VT1200 / VT1200 S is a microtome with a vibrating blade and ispredominately used for sectioning fixed and unfixed specimens during neu-rological research. • To simplify the process ...

20 Operating Manual V1.1 – 05/2007 5. Working with the Instrument 5.2.2 Fine-adjusting the Blade-to-Specimen proximity and Feeding to the Section Thickness The rotary knob 2 for moving the specimen receptacle vertically can be usedboth for fine-adjusting the blade-to-specimen proximity and for adjus...

21 Leica VT1200 and VT1200 S – Microtome 5.2.4 Selecting the Sectioning Parameters SPEED : Blade feed rate – upper display – LED mm/s. The desired blade feed rate can be adjusted from 0.01 – 1.5 mm/s using rotary knob 1: 0.01 – 0.1 in 0. 01mm/s increments0.10 – 0.5 in 0. 02mm/s increments0.50 – 1.5 ...

22 Operating Manual V1.1 – 05/2007 5.3 Control Element on the Control Panel of the VT1200 S The Leica VT1200 S is a fully automatic microtome with a vibrating bladethat can be operated in semiautomatic or automatic sectioning mode. 5. Working with the Instrument Key/setting dial automatic sectioning...

23 Leica VT1200 and VT1200 S – Microtome 5. Working with the Instrument Key/setting dial semiautomatic sectioning mode In semiautomatic sectioning mode,before each cut, a manual feed tothe desired section thickness mustbe carried out using the setting dialfor section thickness.There is no automatic ...

24 Operating Manual V1.1 – 05/2007 5. Working with the Instrument Key/setting dial semiautomatic sectioning mode The blade feed rate can be adjustedfrom 0.01 – 1.5 mm/s: 0.01 – 0.1 in 0.01 mmm/s increments, 0.10 – 0.5 in 0.02 mmm/s increments, 0.50 – 1.5 in 0.10 mmm/s increments. Adjusting the ampli...

27 Leica VT1200 and VT1200 S – Microtome 5. Working with the Instrument Key/setting dial G e n e r a l i n f o r m a t i o nabout the cutting win-dow semiautomatic sectioning mode Not possible. Not possible. Not possible. automatic sectioning mode The horizontal cutting path can bereduced to the spe...

32 Operating Manual V1.1 – 05/2007 5. Working with the Instrument 5.4 Installing the Accessories 2 5.4.1 Installing the Ice Bath and Buffer Tray • A lever ( 2 ), which needs to be pushed forward, is located on the underside of the ice bath ( 1 ). • Now push the tray onto the dovetail holder ( 3 ) fr...

33 Leica VT1200 and VT1200 S – Microtome 5. Working with the Instrument 5.4.3 Preparing a Specimen • Fix the specimen to the specimen plate usingthe cyanoacrylate (included in the standardscope of delivery). • Screw the manipulator ( 6 ) onto the specimen plate, place it in the buffer tray and adjus...

34 Operating Manual V1.1 – 05/2007 5. Working with the Instrument 5.4.4 Installing and Removing the Blade Holder Fig. 19 • Before the blade holder can be replaced, itmust be brought into a 45° inclined position. To do so, insert the size 3 Allen key sidewaysinto the blade holder through the hole ( 8...

35 Leica VT1200 and VT1200 S – Microtome 5. Working with the Instrument 5.4.5 Inserting the Blade • Insert a size 3 Allen key sideways into theblade holder through the hole ( 8 ) and rotate it 90° clockwise. Clamp the blade as follows:• Insert the provided size 3 Allen key from thetop through the op...

37 Leica VT1200 and VT1200 S – Microtome 6.1 Using the VibroCheck with the VT1200 Prior to assembly, the dovetail guide ( 3 ) is brought down to the lowest position using the DOWN key! 1. The user installs the VibroCheck: the VC ispushed along the dovetail guide ( 3 ) past the mark on the base plate...

38 Operating Manual V1.1 – 05/2007 When LED on VC is green and LED in RUN/ STOP is yellow, the blade starts to vibrate with the amplitude set. Speed = 0, amplitude canbe changed at any time. On the (5-digit) display, the deviation in heightamplitude is shown in μm (e.g. 0.9 μm). This val-ue can be e...

39 Leica VT1200 and VT1200 S – Microtome 6. Operating the VibroCheck Prior to assembly, the dovetail guide ( 3 ) is brought down to the lowest position using the DOWN key! 1 . The user installs the VibroCheck: the VC is pushed along the dovetail guide ( 3 ) past the mark on the base plate of the ins...

41 Leica VT1200 / VT1200 S – Microtome 7. Malfunctions: Meanings and Troubleshooting Error/INF No. Error Troubleshooting Remark - Wrong control panel (VT1200 or VT1200 S) - Head does not swing into place. - Timeout while initializ- ing the x-axis. - DC motor of x-axis does not rotate (during ini-tia...

44 Operating Manual V 1.0 – 10/2006 Error/INF No. Error Troubleshooting Remark 7. Malfunctions: Meanings and Troubleshooting - Basic calibration of light barrier is not pos-sible for VibroCheck. - VibroCheck blade search not successful. - Communication error between control unitand VT. - The buffer ...

45 Leica VT1200 / VT1200 S – Microtome 7. Malfunctions: Meanings and Troubleshooting If the 1000 hour limit is exceeded while the interment is in normal operat-ing mode, a flashing "SEr" appears in the upper 3-digit LED for approx. threeseconds after the sectioning process has been ended wit...

46 Operating Manual V1.1 – 05/2007 7.2 Replacing the Main Fuse Caution:Always unplug the power supply before replacing the fuses! • Remove the fuse housing on the right side of the instrument above thepower switch. To do so, push an appropriate tool (small screwdriver)into the slots on the right and...

47 Leica VT1200 and VT1200 S – Microtome Cleaning the blades Always wipe the blade from the back of the blade (sapphire blade)to the cutting edge. NEVER wipe in the opposite direction – risk ofinjury! Clean using an alcohol-based solution or acetone. 8. Cleaning and Maintenance Before each cleaning,...

49 Leica VT1200 and VT1200 S – Microtome 9.1 Extension Accessories for Standard Specimens 9. Ordering Information for Optional Accessories, Consumables and Spare Parts Buffer tray, complete (plastic) • Buffer tray and lid • Magnetic holder for the specimen plate • Specimen plate, non-directional • C...

53 Leica VT1200 and VT1200 S – Microtome 9.1.7 Installing the Magnifier Support or Microscope Support Fig. 49 2 3 4 • First, remove the cover ( 3 ) from the basic in- strument and store it in a safe place. • Insert the provided Allen screws ( a ) into the hole ( 4 ) in the bearing bush. Use the size...

55 Leica VT1200 and VT1200 S – Microtome 9.1.9 Cyanoacrylate Adhesive 9.1.11 Foot Switch 9.1.10 Minichiller (circulating cooler) Insert the foot switch into the uppermost socketmarked "FS" ( 5 ) on the left side of the basic in- strument. • The foot switch performs the START/STOP function. O...

10. EC Declaration of Conformity We herewith declare, in exclusive responsibility, that the instrument Leica VT1200/VT1200 S – Microtome with vibrating blade was developed, designed and manufactured to conform with the • Council Directive 73/23/EEC, (Low Voltage) and • Council Directive 89/336/EEC, ...

57 Leica VT1200 and VT1200 S – Microtome Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual prod-uct delivered has been subjected to a comprehensive quality controlprocedure based on the Leica in-house testing standards, and that the prod-uct is faultless and complies with all...