Page 2 - Manufacturer information
Leica DM750 Manual 2 Published in November 2010 by: Leica Microsystems (Schweiz) AGIndustry DivisionMax Schmidheiny Strasse 201CH-9435 Heerbrugg (Switzerland) Responsible for contents:Dietmar Gnass (Product Compliance)Vincent Vaccarelli (Product Management) In case of questions, please contact the h...
Page 3 - Chapter overview
Leica DM750 Manual 3 Chapter overview Safety regulations 5 The Leica DM750 17 Ready! 20 Set! 30 Go! 43 Care of the Microscope 45 Troubleshooting 48 Dimensions 51
Page 4 - Contents
Leica DM750 Manual 4 Contents Safety regulations 5 Safety concept 6 Symbols used in this instruction manual 7 Important Notes 8 Intended Purpose of the Microscope 9 Instructions on use 10 Health risks and dangers of use 12 Information for the person responsible for the instrument 13 Care instruction...
Page 6 - Safety concept
Leica DM750 Manual 6 Safety concept The individual modules of the Leica DM microscopy series include an interactive CD-ROM with all relevant user manuals in several languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download...
Page 7 - Symbols used in this instruction manual
Leica DM750 Manual 7 Symbols used in this instruction manual Warning of a danger This symbol indicates especially impor-tant information that is mandatory to read and observe. Failure to comply can cause the following: Ϙ Hazards to personnel Ϙ Functional disturbances or damaged inst-ruments Warnin...
Page 8 - Important Notes
Leica DM750 Manual 8 Important Notes Description The Leica DM750 microscope meets today's state of the art of technology. Nevertheless, hazards may still arise during operation. The potential risks are described below. Before installing, operating or using the instrument, it is mandatory to read t...
Page 9 - Intended Purpose of the Microscope
Leica DM750 Manual 9 The Leica DM750 microscope, for which this instruction manual has been writ- ten, is intended for biological routine and research applications. This includes exami-ning specimens taken from the human body for the purpose of gaining information about physiological or pathologic...
Page 10 - Instructions on use
Leica DM750 Manual 10 Instructions on use The Leica DM750 microscope may be used only in closed rooms and must be placed on a solid substrate. The Leica DM750 microscope can be used in clean rooms without any problems. Always position the Leica DM750 microscope so that you can disconnect it from...
Page 12 - Health risks and dangers of use
Leica DM750 Manual 12 Health risks and dangers of use Health risks Workplaces with microscopes facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Depending on the duration of uninterrupted work, asthenopia and musculoskeletal pro...
Page 13 - Information for the person responsible for the instrument
Leica DM750 Manual 13 Information for the person responsible for the instrument Information for the person responsible for the instrument Ϙ Ensure that the Leica DM750 microscope is used only by qualified personnel. Ϙ Ensure that this user manual is always available at the Leica DM750 microscope. Ϙ ...
Page 14 - Care instructions
Leica DM750 Manual 14 Care instructions General instructions Ϙ Protect the Leica DM750 microscope against damp, vapors, acids, alkalis, and corrosive substances. Do not store chemi-cals in the vicinity. Ϙ Protect the Leica DM750 microscope from oil and grease. Never grease or oil mechani-cal parts o...
Page 15 - Accessories, maintenance and repair
Leica DM750 Manual 15 Accessories, maintenance and repair Accessories Only the following accessories may be used with the Leica DM750 microscope: Ϙ The Leica accessories described in this user manual. Ϙ Other accessories, provided that these have been expressly approved by Leica as being technically...
Page 16 - Electrical data and ambient conditions
Leica DM750 Manual 16 Electrical data and ambient conditions In order to maintain this condition and to ensure safe operation, the user must follow the instructions and warnings contained in this instruction manual. Environment Temperature for use +10 °C … +40 °C Storage temperature -20 °C … +52 °...
Page 18 - Introduction
Leica DM750 Manual 18 Thank you for purchasing the Leica DM750 Compound Microscope from Leica Microsys-tems. This model’s exclusive design features and full range of accessories make it a truly versatile, high quality-instrument for educatio-nal and routine clinical applications. The instrument is...
Page 19 - Unpacking
Leica DM750 Manual 19 Ϙ Carefully remove the microscope and any components from the packing carton. Ϙ Verify that all components are intact. Ϙ Check the components against the planned configuration. Ϙ Optional items such as contrast accessories, camera adapters, cameras, and carrying cases are not s...
Page 21 - Substage Illumination
Leica DM750 Manual 21 Substage Illumination The Leica DM750 is available with two types of Substage Illumination. Identify which type of Illumination you have, as this will be important to know later. Type 1: Standard illumination Adjustable condenser centering with provided tool. Type 2: Koehler ...
Page 22 - Attaching the viewing Tubes
Leica DM750 Manual 22 Tools used Ϙ Allen key There are two types of viewing tube. Identify which viewing tube you have: Type 1: Leica EZ Viewing Tube with integrated eyepieces. Type 2: Standard viewing tube with separate eyepieces 1. Loosen the setscrew (on top of the stand) using the Allen key pr...
Page 23 - Leica EZ Viewing Tube – integrated eyepieces
Leica DM750 Manual 23 2. Reinstall the Leica EZ Viewing Tube onto the stand. 3. Tighten the captive wingscrew using the wrench included in the delivery package. Leica EZ Viewing Tube – integrated eyepieces To rotate the Leica EZ viewing tube, either loosen the set screw on the stand or replace the...
Page 25 - Leica Standard Viewing Tube – separated eyepieces
Leica DM750 Manual 25 Leica Standard Viewing Tube – separated eyepieces 2. Secure the eyepieces in the tubes by tighte- ning the silver screws at the bottom of the tubes using a standard Phillips screwdriver (not included in the delivery package). The eyepieces will still rotate, but they will be ...
Page 26 - Eyecups
Leica DM750 Manual 26 If you wear eyeglasses for microscope viewing, keep the rubber eyecups folded down. If you do not wear eyeglasses, you may find it useful to unfold the rubber eyecups in order to help block out ambient room light. Eyecups If you have purchased a standard microscope configur...
Page 28 - Installing the Substage Condenser
Leica DM750 Manual 28 Installing the Substage Condenser Substage Condenser The Leica DM750 has an open substage condenser mount, therefore the conden- ser needs to be installed. 1. Move the specimen stage upwards as far as it will go by using the coarse focusing knob on the side of the microscope....
Page 31 - Turning on the microscope
Leica DM750 Manual 31 Work Surface Always use your microscope on a hard, stable surface. Power Cord If the power cord is not already atta-ched, attach it securely to the back of the microscope. Setting the Illumination Intensity Set the illumination to the lowest setting to start with, using t...
Page 32 - Complete Condenser Centering
Leica DM750 Manual 32 If you have purchased a standard Leica DM750 configuration, the condenser has already been pre-centered by Leica Microsys-tems. 1. Open the condenser aperture by rotating the knurled ring on the condenser to the right. Make sure that the condenser is in the highest position...
Page 33 - Using the Condenser
Leica DM750 Manual 33 Using the Condenser The condenser is furnished with an iris diaphragm, which can be adjusted to match the effective numerical aperture of the objective 1. To open and close this diaphragm, simply turn the knurled ring on the condenser to the right or left so that the line on ...
Page 34 - Prepare to View a Specimen Slide
Leica DM750 Manual 34 1. Position a specimen slide on the specimen stage by sliding it under the slide grips. Slide grips hold the slide in place. 2. Using the X/Y-stage control, position the specimen slide such that a part of the speci-men is under the objective used. Prepare to View a Specimen S...
Page 35 - Focusing
Leica DM750 Manual 35 1. Rotate the objective nosepiece (using the knurled ring) in such a way that the objec-tive with the lowest magnification level is rotated into the working position. 2. Move the specimen stage upwards by turning the coarse focusing knob as far as it will go to the maximum posi...
Page 36 - Viewing Tube Adjustment
Leica DM750 Manual 36 Adjust the eyetubes 1. Adjust the tubes to your interpupillary distance. Fold or unfold the eyetubes to decrease or increase the distance between the eyepieces until you see one illuminated circle. The viewing tubes maintain a constant tube length for all interpupillary setti...
Page 38 - Koehler Configuration
Leica DM750 Manual 38 If your Leica DM750 is fitted with a field diaphragm for Koehler Illu-mination, use the following procedure to assure condenser cente- ring and focus. 1. Connect the Koehler field diaphragm to the base of the microscope so that the diaphragm leaves are in the field of view wh...
Page 40 - Oil Immersion Technique
Leica DM750 Manual 40 1. Search for the area on the specimen slide that you want to study. 2. Move the specimen stage down to the lowest position using the coarse adjust-ment knob. 3. Add a drop of immersion oil from Leica to the area of the specimen slide you are studying. 4. Rotate the oil immersi...
Page 42 - Time Delay Shutoff
Leica DM750 Manual 42 The Leica DM750 is equipped with a time delay shutoff capability, which automati- cally turns the illumination off after 2 hours of no changes in the intensity control. Ϙ All four-position nosepiece stands have the time delay shutoff enabled as a default (mostly educational a...
Page 45 - Care of the Microscope
Page 46 - General maintenance
Leica DM750 Manual 46 General maintenance Storage and handling Always use both hands to carry the microscope. There is a handle on the back of the microscope and an undercut in the front for this purpose. Ϙ The cord wrap allows you to wrap the cord in such a way that only the length you need is ex...
Page 48 - Troubleshooting
Page 51 - Dimensions