Leica DM IRB - Manual

7 The manual is multi-lingual. Due to the spiralbinding you can turn the language version youwant to the front. The DM IR is available both as a life sciencesmicroscope and as a metallographic/industrialmicroscope. In cases where the function andoperation are identical, the same text andillustration...

8 Make sure that only fuses of the specified typeand rating are used as replacements. It isforbidden to use mended fuses or to short-circuit the fuse holder. n. b.: The instruments and accessories describedin this manual have been safety-tested andchecked for possible hazards.Before modifying the in...

10 The new DM IRB is the logical furtherdevelopment of the successful invertedresearch microscope from Leica. It is usedfor examinations of cells and tissue, formicromanipulation and microinjection tech-niques all the way through to microdissection orconfocal microscopy. The DM IRB has universalappl...

11 The microscope and its components Fig. 1 – 21 Binocular phototube, 2 Eyepiece adapter tube, 3 Eyepieces, 4 Tube mount (tube change interface), 5 Tube port for photo/TV connection, 6 Beamsplitter switch, 7 Mains switch, 8 Brightness adjustment, 9 Lateral TV port, 10 Coaxial coarse and fine drive, ...

15 n. b.: Lamphousings* and power units* must beplaced at least 10 cm away from the wall andfrom flammable objects. Installation site The microscope should be used in a dust-freeroom which is free of oil and chemical fumesand extreme humidity. Also, the workplaceshould not be exposed to major temper...

17 Fig. 3 Assembly tools 1 Cross-tip screwdriver*, 2 Hexagonal screwdriver, 3 mm, 3 Centring keys, 2 mm*, 4 Centring keys, 1.5 mm*, 5 Allen key, 3 mm*, 6 Allen key, 2.5 mm* (short version) Assembly Assembly of the transmitted light illuminationcolumn Wipe off the interface surface (4.3) with a drycl...

43 Input menu of learn mode After switching on, the microscope is in the nor-mal operation mode. Normal operation mode The learn mode is switched on with the“LEARN” key.The objective nosepiece rotates through 180 ° so that the current objective is in the mostaccessible position (furthest to the righ...

44 Turn the IC turret (situated under the objectivenosepiece) until it clicks into the brightfieldposition (H).Operate the focus handwheel until the letter Happears on the display panel as well. Turn the ICturret by a quarter of a rotation into the nextclickstop area. The message “IC prism 2”appears...

45 Now screw the objective with the lowestmagnification into the nosepiece opening whichis furthest to the right. Display: Objective magnification By turning the focus handwheel, select thenumber in the electronic display that corre-sponds to the magnification of the objective. Display: Phase contra...

48 Z-drive Objective Nosepiece move- keys keys ment when the Chosen function right handwheel is turned clockwise right left up right left down left right up left right down Possible combinations for user adjustment By turning the focus wheel, choose the one ofthe four possible combinations that suit...

49 Learning other filters:Select the next filter cube position by pressingone of the “FLUO” keys on the control panel ofthe FLUO module. Insert the filter cube andselect the corresponding name in the display.Repeat the procedure for any other filters.Unoccupied positions are given the code “-”. To c...

51 Operating modes Contamination of the dry objectives is preventedby the fact that the objective nosepiece isalways lowered before objectives are changed. When switching from one operating mode to theother, please proceed as follows: Starting in the DRY mode:(Display at the bottom right in the LD d...

53 Brightness adjustment Instead of a potentiometer, Leica DM IRB/Emicroscopes are equipped with an incrementaltransducer for brightness adjustment. Thismeans that the adjustment wheel is automati-cally moved from clickstop to clickstop andtherefore has no end stops. After it is switched on, the mic...

54 Electronic focus The controls of the electronic focus are: – The focusing handwheels, conventionally positioned on both sides of the microscope. – Two keys (focus keys) for fast lowering of the objective nosepiece and returning to the focalplane. The keys are in a convenient position infront of t...

64 16 24 25 13 19 2223 2 3 5 120 4 28 Fig. 54␣ –␣ 551 Binocular phototube, 2 Eyepiece tube, 3 Eyepieces, 4 Tube mount (tube interface), 5 Tube port for photo/TV connection, 6 Beamsplitter switch, 7 Mains switch, 8 Brightness adjustment, 9 Lateral TV port, 10 Coaxial coarse and fine drive, 11 Fluores...

70 Immersion objectives OIL: Only use DIN/ISO standard immersion oil. n. b.: Observe the safety information on the im-mersion oil! W: Water immersion. The special water im- mersion objectives with ceramic front part canbe used for all hydrous solutions. IMM: Universal objective for water, glycerine ...

71 Operation of transmitted light Setting the aperture diaphragm The aperture diaphragm determines the lateralresolution, field depth and contrast of themicroscope image. The best contrast isobtained when the apertures of the objectiveand the condenser are roughly the same.When the aperture diaphrag...

74 Operation of phase contrast Set the light ring (64.2) in the condenser discthat corresponds to the objective engraving(PH2). Open the aperture diaphragm (= pos. PH).Move the Bertrand lens into the light path =pos. B by turning the knurled knob and focus theannular structures with the lever (Fig. ...

76 Operation of transmitted light darkfield Darkfield observation Darkfield observation is not possible withcondenser 0.30 S70, with condenser 0.53 S23 it ispossible from 5x objective magnification, themax. usable objective aperture is 0.40. Withcondenser 0.90 S1, DF observation is possiblefrom obje...

77 DL polarisation Polarisation contrast for examining birefringentspecimens is possible with condenser 0.30 S70with objective magnifications from 2.5x to 40x,with condensers 0.53 S23 or 0.90 S1 from 5x or10x to 100x. A P 1.40 OIL S1 condenser top is alsoavailable for extremely hgh resolution. Cross...

81 The BG 38 filter should always be used forphotography.When not looking through the microscope,always block the incident light path to preventspecimens fading. Push the switch rod in all theway. The 3 clickstop positions of the switch rod mean: Switch rod Stop v Incident light path blocked(light s...

88 Light filters Up to max. 3 light filters can be inserted in the filter holder (1.16). They can be switched in and outthe light path as required. Filter Use Grey filter Grey filters (neutral density filters) are used toattenuate the light without influencing thecolour temperature. The engraved val...

89 The original is imaged 2␣ :␣ 1 in the intermediateimage plane of the microscope. A distance ofe. g. 5 mm in the slide overlay is enlarged to10 mm in the intermediate image plane of themicroscope. The overlay is only possible in beamsplitterposition 50/50 (switch rod) in the middle positionof the ...

90 Stand lamps, cold-light illuminators and fibre-optic lamps, etc. are suitable sources for micro-scopy.The image is observed in the microscope tubeand focused by turning the knurled ring (73.10).The magnification can be changed continuouslyin a range of 1 : 4 by adjusting the zoom ring(73.7).When ...

97 Operation of LMC Leica modulation contrast (LMC) is a specialform of oblique illumination based on theprinciple of Hoffmann modulation contrast. In this technique, the phase gradients of anunstained specimen are converted into dif-ferences in amplitude with the aid of a modu-lator.This gives a th...

98 The principle Leica modulation contrast (LMC) is based on theprinciple of Hoffmann modulation contrast.This imaging technique is particularly suitablefor unstained, colourless objects with littleimage contrast.Such objects change the phase of the lightwhen it passes through them.The conversion of...

99 Components The components LMC consists of the following components: S40/0.50 LMC condenser The condenser (order no. 521 225) is suppliedwith a condenser disc to accommodate 3 LMCdiaphragms, plus two phase contrast light ringsand a brightfield position (3x LMC, PH1, PH2,brightfield).Adhesive label...

102 Areas of application On the Leica DM IRB/E microscope, LMC isparticularly suitable for life science applica-tions. Use of birefingent materials Transparent, living cultures in petri dishes canbe observed in three dimensions, for example. Use of a micromanipulator The long free working distance o...

103 n. b.: Before cleaning and maintenance work, re-member to disconnect from the mains!Protect electric components from damp! Microscopes in warm and humid climates needspecial care to keep them free of fungus.The microscope should be cleaned every time itis used and the microscope optics should be...

105 Electric errors These may be: 1. The lamp on the microscope does not work.2. There is no power. Check the following possible causes: The on/off switch does not respond(no illumination): ● Check that all mains cables are properly con-nected. ● Make sure that there is power at the socketsyou are u...

112 Storage Protect your microscope from dust by putting onthe cover after each work session. The microscope must be kept in a cupboard inwhich the temperature is ≥ 5 ° C above room temperature. The cupboard must have ven-tilation holes which are plugged with cottonwool, for example, to keep dust ou...

113 All techniques, not only in microscopy, aresubject to limits of performance due to basicphysical laws and principles of eye physiology.The following information should therefore beremembered when using the microscope. Performance data of objectives The Leica DM IRB microscope is based on tubelen...

127 Main wearing and spare parts, tools Order No.Part no. Component Used for Spare lamps500 974 Halogen lamp 12 V 100 W Lamphousing 105 500 137 Ultra high pressure Hg lamp 50 W Lamphousing 106 z 500 138 Ultra high pressure Hg lamp 100 W Lamphousing 106 z in preparation Ultra high pressure Hg lamp 10...

128 EU Conformity declaration We hereby declare that the product specifiedbelow conforms in its design and constructionas well as the model we have put on the marketto the relevant safety and health regulations laiddown by the European Union.This declaration will cease to be valid if theinstrument i...