Page 3 - © Leica Biosystems Nussloch GmbH
3 Leica CM1950 – Cryostat Published by:Leica Biosystems Nussloch GmbHHeidelberger Str. 17 - 19D-69226 NusslochGermany Phone: +49 (62 24) 143-0Fax: +49 (62 24) 143-2 00 eMail: [email protected] Internet: http://www.histo-solutions.com These are determined only by the contract provi-s ...
Page 4 - Table of contents
4 Operating Manual V 1.1 – 05/2007 Table of contents 1. Important Information ......................................................................................................................... 6 1.1 Symbols Used in This Manual and Their Meaning ...................................................
Page 6 - Important Information; Caution – UVC radiation!; Instrument Type; Any other use of this instrument is considered; Qualification of Personnel
6 Operating Manual V 1.1 – 05/2007 1. Important Information 1.1 Symbols Used in This Manual andTheir Meaning Notesi.e. important user information appearin a gray box and are marked by an in-formation symbol. Caution – UVC radiation! Figures in brackets refer to item num-bers in drawings or to the dr...
Page 7 - Safety and Design
7 Leica CM1950 – Cryostat 2.1 General Safety Instructions The CM1950 is a cryostat with an encapsulated microtome and separatespecimen cooling. It is primarily used for work in the area of fast-cut diag-nostics. The displays and instrument controls are easy to operate due totheir largely self-explan...
Page 8 - Opening the packaging
8 Operating Manual V 1.1 – 05/2007 2. Safety and Design Opening the packaging 1. Remove the 3 screws ( 1 ) on each of the two sides of the box ( 7 ) at the top. 2. Remove the 2 screws on each of the 4 hinges ( 2 ) on the front and rear side of the box. Do NOT remove the screws of the hinges on the t...
Page 9 - Removing the packaging; rear; Transport to the Installation Location; The instrument must be transported in an upright position.
9 Leica CM1950 Cryostat 2. Safety and Design Removing the packaging 1. Remove the cover ( 17 ) and place it on the floor with the foam parts facing downwards. - The cover will be used as a ramp later. 2. Remove the 4 foam parts ( 6 ) by pulling them upwards. 3. Open the box ( 7 ) on its side hinges ...
Page 10 - Microtome knives; Do not place a knife on a table with the cutting edge facing upwards!; Safety Features; before
10 Operating Manual V 1.1 – 05/2007 To prevent adverse health effects from UV radiation, the UV disinfec-tion cycle can be started only after the sliding window has been prop-erly closed. Closing the window activates the corresponding safetyfeatures. The consistent use of these safety features and s...
Page 11 - Securing/Locking the Handwheel; To release the handwheel, press the lever (; Knife guard
11 Leica CM1950 – Cryostat 2.4 Securing/Locking the Handwheel Always lock the handwheel and cover the cutting edge with theknife guard prior to manipulating the knife or specimen, changingthe specimen, or taking a break. To lock the handwheel, press the lever ( 1 ) outward. Continue turning the hand...
Page 12 - It is not necessary to remove the microtome for disinfection.; Cleaning, Disinfection – Turning the Instrument Back On
12 Operating Manual V 1.1 – 05/2007 2. Safety and Design • When disinfecting the instrument, take appropriate protective measures(gloves, mask, protective clothing, etc.). • When using detergents and disinfectants please comply with the safetyprecautions of the disinfectant manufacturer. • The integ...
Page 13 - Removal of the Microtome; Replacing the fuses; Handling Specimens – Defrosting; Before defrosting the cryochamber remove all samples!
13 Leica CM1950 Cryostat 2. Safety and Design 2.7 Removal of the Microtome • The microtome is encapsulated and therefore does not require removalby the user. 2.8 Maintenance Replacing the fuses • Turn the instrument off and disconnect the power plug before replacingthe fuses. • Use only the fuse typ...
Page 14 - Technical Data; Type; Microtome; Electric coarse feed; Cryostat
14 Operating Manual V 1.1 – 05/2007 3. Technical Data ② according to IEC-1010; UL 3101 Type Nominal voltage (±10 %)Nominal frequencyPower drawMax. start-up current for 5 sec.Protective classCircuit breaker:(MDA made by Schurter)Pollution degree ② Overvoltage installation categoryHeat emission (max.)...
Page 15 - Refrigeration system
15 Leica CM1950 – Cryostat 3. Technical Data Refrigeration system CM1950 , 50 Hz CM1950 , 60 Hz Cryochamber Temperature range 0 °C to -35°C ± 5 K, adjustable in 1K increments, 0 °C to -35°C ± 5 K, adjustable in 1K increments at ambient temperature of 20 °C ambient temperature of 20 °C Cooling time t...
Page 16 - Standard Delivery; A choice of different knife holders is available for the CM1950 .; Basic instrument WITHOUT motor and WITH extraction,
16 Operating Manual V 1.1 – 05/2007 4. Standard Delivery A choice of different knife holders is available for the CM1950 . Compare the delivered components with the parts list and your order. Should youfind any discrepancies, please contact your Leica sales office without delay. Basic instrument WIT...
Page 18 - General Overview
18 Operating Manual V 1.1 – 05/2007 5. General Overview Castors for safe transport over short distances Handwheel in12 o’clockposition On/off switch, also circuitbreaker Align adjusting feet for secureupright position Unscrew and align adjusting feet after transport Bacterial filter (optional, only ...
Page 19 - Control Panel Fields and Cryostat Chamber; - Position holder on freeze shelf
19 Leica CM1950 – Cryostat 5. General Overview 5.1 Control Panel Fields and Cryostat Chamber 1 2 3 1 - Control panel field 1: Extraction, temperature and time control, illumination, UV disinfection 2 - Control panel field 2: Electric coarse feed (sectioning and trimming thickness adjustment) 3 - Con...
Page 20 - Installation; Site Requirements
20 Operating Manual V 1.1 – 05/2007 6. Installation 6.1 Site Requirements The place of installation must meet the following requirements: • No direct sunlight. • Power supply at a distance no greater than approx. 3 m. • No drafts (air condition outlets etc.). • Even floor. • Vibration-free floor. • ...
Page 21 - Transport to the Site; Transport the instrument to the desired location.
21 Leica CM1950 – Cryostat 6. Installation 6.2 Transport to the Site • First, check if the location meets the conditions specified in "Siterequirements". • Transport the instrument to the desired location. • Observe the following: The instrument must be transported in an upright position or ...
Page 22 - Assembling the Handwheel; Transport with a fork lift
22 Operating Manual V 1.1 – 05/2007 • Insert the pin ( 1 ) of the handwheel shaft into the hole ( 2 ) of the handwheel. • Tighten the screw ( 4 ) using the No. 6 Allen key. • Place the protective cap on the screw ( 4 ). To dismount, proceed in reverse order. 1 2 4 6.3 Assembling the Handwheel Fig. 1...
Page 24 - Connection to Power Supply System; Do not connect any other consumers to this electric circuit.; Only for instruments sold in Japan; ) to select the frequency corresponding to the conditions of
24 Operating Manual V 1.1 – 05/2007 6.4 Connection to Power Supply System After transporting, wait at least 4 hours before turning the instrument on. This waiting period isnecessary to allow the compressor oil, which may have been displaced during transport, to re-turn into its original position. Fu...
Page 25 - Installing Accessories/Inserting Chamber Accessories; ) for the footrest on the
25 Leica CM1950 – Cryostat 6.5 Installing Accessories/Inserting Chamber Accessories 6.5.1 Installing the Adjustable Footrest (Optional) • To install the optional footrest, unscrew thescrews ( 1 ) using the No. 3 Allen key provided. 1 • Attach the holders ( 2 ) for the footrest on the left and right ...
Page 26 - ) that prevent the interior of the; ) in front of the bore and tighten the screws/
26 Operating Manual V 1.1 – 05/2007 6.5.2 Installing the Storage Systems (Optional) 6.5.3 Shelf, Movable (Optional) Attach the rod for the shelf to the inner front sideof the cryostat housing using the provided screws( 1 ) and the No. 3 Allen key, then attach the caps ( 3 ). (The rear side of the mo...
Page 27 - and Adjusting the Clearance Angle; Set the knife or blade holder (
27 Leica CM1950 – Cryostat 6. Installation 6.5.5 Installing the Heat Conductor Block, Stationary (Optional) Fig. 24 6.5.6 Installing the Knife/Blade Holder and Adjusting the Clearance Angle For temperature reasons, install theknife/blade holder on an appropriatebase. • Set the knife or blade holder ...
Page 28 - traction hose connecting piece (
28 Operating Manual V 1.1 – 05/2007 Reasons: 1. To prevent section waste from falling into the opening. 2. To prevent cold from escaping from the chamber. 3. To prevent moisture from penetrating into the chamber. 6. Installation 6.5.7 Inserting/Changing the Bacteria Filter 6.5.8 Assembling the Filte...
Page 29 - of the chamber suction nozzle (; to the filter
29 Leica CM1950 – Cryostat 6.5.9 Installing the Section Extraction (Optional) – Use with Blade Holder CE Only 6. Installation If the extraction is not used for a long time, it is absolutely necessary to clean the extraction hose in orderto ensure maximum extraction capacity. To do so, place the hose...
Page 30 - Instrument Controls; Control Panel Fields on the CM1950 – Control Panel Field 1; EMERGENCY STOP switch to the right of control panel field 1
30 Operating Manual V 1.1 – 05/2007 7. Instrument Controls Display of actual and target specimenhead temperatures Display of actual and target chambertemperatures Display of real time, defrost timeand error messages Max-Cool button toselect the maximumlow temperature ofthe specimen headdirectly (-50...
Page 32 - Setting section/trim thickness; Retraction
32 Operating Manual V 1.1 – 05/2007 For trimming values above a sec-tion thickness of 200 μm, the dis-play flashes to expressly notifythe user of thick sections! Control Panel Field 2 – Electric Coarse Feed, Sectioning and Trimming Thickness Display 1 Specimen head forwards – pressing brieflyadvance...
Page 33 - slow
33 Leica CM1950 – Cryostat 7. Instrument Controls Coarse feed functions The two-speed electric coarse feed is designed to quickly position the spec-imen in relation to the knife. The buttons with double arrows move the spec-imen at 900 μm/s; the buttons with single arrows move it at 300 μm/s. Retrac...
Page 34 - Control panel 3 – Motorized sectioning (optional)
34 Operating Manual V 1.1 – 05/2007 Sectioning modes The instrument can be used in manual and motorized modes.The following settings are available: • Single stroke (SINGLE) or continuous stroke (CONT .) in automatic mode, as well as • ROCK (sectioning using handwheel) in manual mode. When switching ...
Page 35 - Disinfection
35 Leica CM1950 – Cryostat 7. Instrument Controls Disinfection Duration - 30 min Duration - 180 min UVC key - to activate / deac-tivate the disinfection cycleand/or to acknowledge in-terruption of a disinfectioncycle. To start disinfection the sliding window must becompletely closed.• P r e s s U V ...
Page 36 - Working with the Instrument; Preparing Cutting Tools, Specimen Discs and Preparation Aids; Close the sliding window.
36 Operating Manual V 1.1 – 05/2007 8. Working with the Instrument 8.1 Preparing Cutting Tools, Specimen Discs and Preparation Aids The knives are extremely sharp! Handle with care!Never try to catch a falling knife! • Place working materials such as the blade box or knives (in the knife case), brus...
Page 37 - Configuring the Parameters; Turn the lamp on or off.; Refer to page 52
37 Leica CM1950 Cryostat 8. Working with the Instrument 8.3 Configuring the Parameters The instrument must be switched on at least 5 hours before theplanned use. • Turn the lamp on or off. • This button activates or deactivates the manual defrost of the coolingchamber, specimen head or freeze shelf....
Page 38 - Setting the time; Programming the specimen temperature
38 Operating Manual V 1.1 – 05/2007 Setting the time • The clock time is set with the +/- buttons in the control panel markedwith the clock symbol.To do so, set the current time using the "+" or "-" button below the smallclock symbol.Pushing the "+" or "-" button for ...
Page 39 - Programming the defrost cycle
39 Leica CM1950 Cryostat 8. Working with the Instrument Programming the defrost cycle • Set the beginning of the automatic defrost cycle.The automatic defrost cycle takes place once within 24 hours.It is set with the "+"/"-" buttons on the right of the panel with the clocksymbol. The...
Page 40 - Manual defrosting of the cryochamber
40 Operating Manual V 1.1 – 05/2007 8. Working with the Instrument Manual defrosting of the cryochamber • Push the manual defrost button (with the melting snowflake) on the leftover the key button to activate the defrost cycle of the cryochamber ondemand. • Activation is confirmed by an audible sign...
Page 41 - Entering the trimming thickness
41 Leica CM1950 Cryostat 8. Working with the Instrument Entering the trimming thickness To switch from a trimming section thickness for research applica-tions (1-600 μm) to a thickness for clinical applications (10, 20,30 or 40 μm), press and hold down the TRIM/SECT key whileswitching on the instrum...
Page 42 - Working with the Precooled Cryostat; Cut the specimen to size outside of the cryostat.
42 Operating Manual V 1.1 – 05/2007 8. Working with the Instrument 8.4 Working with the Precooled Cryostat 8.4.1 Preparatory Work • Lock the handwheel in the top (12 o’clock) position. • Cut the specimen to size outside of the cryostat. • Select the precooled specimen disc, cover it with freezing co...
Page 43 - Insert the extraction hose with the black adapter.
43 Leica CM1950 – Cryostat 8. Working with the Instrument 8.4.2 Trimming with Extraction – Anti-roll Guide Installed • Remove the silicone stopper from the filter cover (and keep it in a safeplace). • Insert the extraction hose with the black adapter. • Fold the anti-roll guide to the side and faste...
Page 44 - A Optimal area for trimming and sectioning
44 Operating Manual V 1.1 – 05/2007 8. Working with the Instrument For motorized sectioning, always begin at low speed for safetyreasons. • Optimize the vacuum settings if necessary. • Press the "VAC" button to enable the vacuum extractor. The LED in the"VAC" button is lit while the ...
Page 45 - ENABLE
45 Leica CM1950 Cryostat 8. Working with the Instrument • Activate trimming mode. • Select the trimming thickness. • Switch on the VAC and select a low extraction level (between 1 and 2). • Start trimming by manually moving the handwheel or press the RUN/STOP and ENABLE buttons simultaneously to sta...
Page 46 - Trimming, 1 x 1cm specimen
46 Operating Manual V 1.1 – 05/2007 A few rules: • Always begin at a low extraction level, then slowly increase it. • Do not use high extraction levels unless absolutely necessary. • Different specimen sizes require different extraction levels. • The faster the trimming or sectioning speed, the lowe...
Page 47 - After trimming or sectioning:
47 Leica CM1950 Cryostat 8. Working with the Instrument After trimming or sectioning: Specimen: • Unclamp and thaw. • Immerse in fixative for further processing. Cleaning • Use the brush to sweep up the section waste (section waste tray) andremove it from the cryostat (follow applicable laboratory r...
Page 48 - Sections smear; Troubleshooting; Problems During Work
48 Operating Manual V1.1 – 05/2007 Problem Causes Remedies Frost on chamber walls andmicrotome Sections smear Sections splinter Sections not properlyflattened Sections not properlyflattened despite correcttemperature and correctlyaligned anti-roll plate Sections curl on anti-rollplate Scraping noise...
Page 49 - Ridged sections
Leica CM1950 Cryostat 49 Problem Causes Remedies Ridged sections Chatter during sectioning Condensation on anti-rollplate and knife duringcleaning Anti-roll plate damagedafter adjustment Thick-thin sections Knife damaged.Edge of anti-roll plate damaged. Specimen insufficiently frozen ontothe specime...
Page 50 - Prepare new specimen.
50 Operating Manual V1.1 – 05/2007 Problem Causes Remedies Blunt knife.Inappropriate section thickness.Incorrect clearance angle.Microtome not dried thoroughlyenough.Dried specimen. Anti-roll plate is too warm orincorrectly positioned.Fat on the corner or edge of anti-rollplate.Anti-roll plate not c...
Page 51 - Lamp does not work
Leica CM1950 Cryostat 51 Problem Causes Remedies No or insufficientrefrigeration of thecryochamber Sliding window collectscondensation No or insufficientrefrigeration of thespecimen Lamp does not work Both disinfection LEDs flashalternately Cooling system or electronic drivedefective. Air humidity a...
Page 52 - Temperature Selection Chart (in minus °C)
52 Operating Manual V 1.1 – 05/2007 10. Temperature Selection Chart (in minus °C) Tissue type Chamber temperature Specimen head temperature Spleen -15 °C to -20 °C -11 °C Liver -10 °C -20 °C -15 °C off to -15 °C Intestine -10°C -20 °C -15 °C A*: off to -20 °C E*: -20 °C Heart -10 °C A: -20 °C E: -20...
Page 53 - Ordering Information; Optional Accessories
53 Leica CM1950 – Cryostat 11.1 Ordering Information Specimen disc ø 20 mm assembly .................................................... 0477 43739 Specimen disc ø 30 mm assembly .................................................... 0477 40044 Specimen disc ø 40 mm assembly .............................
Page 56 - Integrated lateral shift and stable base
56 Operating Manual V 1.1 – 05/2007 The knife guard is foldedaway in the detail view.Knife guard folded away Fig. 43 Four possiblesettings Blade rest (ruler) for low-profileblades 1 2 1 2 6 Fig. 44 11. Optional Accessories Red elements on the blade or knifeholders, such as the knife guard andejector...
Page 58 - Adjusting the Knife Holder with Anti-roll Guide
58 Operating Manual V 1.1 – 05/2007 11. Optional Accessories Adjusting the Knife Holder with Anti-roll Guide You can adjust the height of the anti-roll guidesystem using the knurled nut ( 1 ): • If you turn the nut counterclockwise, the anti-roll guide system moves toward the blade. • If you turn th...
Page 59 - while holding it down by the lever (
59 Leica CM1950 – Cryostat Cleaning the knife holder CNDaily cleaning For daily cleaning, it is enough to fold theanti-roll guide system forwards and removethe section waste from the knife holder usinga dry brush. Please use a cold brush, asotherwise the section waste will thaw andstick to the knife...
Page 61 - ) is fixed and integrated into
61 Leica CM1950 – Cryostat Knife holder CNZ with anti-roll guide • Pressure plate permits the full length of theknife to be utilized. • Use hard metal and steel knives here. Fig. 52 11. Optional Accessories The height of resharpened knives must be adjusted using knurledscrews (3) (approx. 1 mm under...
Page 62 - Optional accessories
62 Operating Manual V 1.1 – 05/2007 Fig. 56 Bacterial filter • Bactericidal filter 350/5865, pack of 1 unit. Rec-ommendation: replace bactericidal filter everythree months. (note date on filter when installing it) Anti-roll guide system Anti-roll plate (with glass stage plate) Available with various...
Page 64 - system; O-rings in various colors
64 Operating Manual V 1.1 – 05/2007 Fig. 60 A Heat extractor with parking station, stationary • for installation in the cryostat - consisting of:support device, heat extractor and parking sta-tion B Heat and cold extractor • suitable for two different applications: extractscold when stored in warm e...
Page 65 - for use with motorized instruments only; for use with blade holder CE
65 Leica CM1950 – Cryostat Footswitch, for use with motorized instruments only Fig. 63 Fig. 66 Fig. 65 11. Optional Accessories Brush shelf, for use with blade holder CE Fig. 64 Adjustable support, individually height-adjustable foot rest with 5 set-tings. Storage system, movable for installation in...
Page 66 - Maintenance and Cleaning; General Maintenance; once
66 Operating Manual V 1.1 – 05/2007 12. Maintenance and Cleaning 1 Fig. 67 12.1 General Maintenance The microtome is virtually maintenance-free. To ensure a smooth operationof the instrument over several years we recommend the following:• At least once a year, have the instrument inspected by a qual...
Page 67 - of the condenser on the bottom right-hand side of the
67 Leica CM1950 Cryostat 12. Maintenance and Cleaning Basically, we recommend UV disinfection (see page 35 for detailedinstructions).For easy-to-use spray disinfection we recommend Leica Cryofect. (Cryofectis not available in all countries!)The cryostat has to be disinfected after each daily use. Co...
Page 70 - Decontamination Certificate; Leica accepts that it is not possible to decontami-; Yes; This equipment has been cleaned and decontaminated:; Yes No
70 Operating Manual V 1.1 – 05/2007 13. Decontamination Certificate (master) Dear Customer,Any product which is to be returned to Leica Microsystems or serviced on site, must be cleanedand decontaminated in the appropriate manner. Leica accepts that it is not possible to decontami- nate for CJD and ...
Page 71 - Important - to avoid refusal of shipment:
71 Leica CM1950 – Cryostat 13. Decontamination Certificate (master) Leica Biosystems Nussloch GmbHHeidelberger Str. 17-1969226 Nussloch, Germany Phone: ++49 (0) 6224 143 0Fax: ++49 (0) 6224 143 200 www.leica-microsystems.com Important - to avoid refusal of shipment: Place one copy in the unit prior ...
Page 72 - EC Declaration of Conformity; President Biosystems Division
We herewith declare, in exclusive responsibility, that the Leica CM1950 Cryostat was developed, designed and manufactured to conform with the• Council Directive 2006/95/EC (Low Voltage), • Council Directive 89/336/EEC, Appendix I (Electromagnetic Compatibility) and • European council Directive 98/79...
Page 73 - Warranty and Service; Warranty
73 Leica CM1950 Cryostat 15. Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual productdelivered has been subjected to a comprehensive quality control proce-dure based on the Leica in-house testing standards, and that the product isfaultless and complies wit...
Page 74 - People's Republic of China
74 Operating Manual V 1.1 – 05/2007 16. People's Republic of China
