Leica dm750- Manuals

Leica DM750 P Manual 6 Symbols used in this operating manual Warning of a danger •  This symbol indicates especially impor-tant information that must be read and complied with. Failure to comply can cause the following: Ϙ Hazards to personnel Ϙ Functional disturbances or damaged instru-ments Warning...

Leica DM750 P Manual 7 Important Notes Description The Leica DM750 P microscope meets today's state of the art of technology. Nevertheless, hazards may still arise during operation. The potential risks are described below. •  Before installing, operating or using the instruments, it is required to r...

Leica DM750 P Manual 8 Instructions on use Intended use The Leica DM750 P microscope is an optical instrument which uses magnification and illu-mination to improve the visibility of objects. It is used for observing and documenting. The Leica DM750 P microscope may be used only in closed rooms and m...

Leica DM750 P Manual 10 Health risks and dangers of use Health risks   Workplaces with microscopes facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Depending on the duration of uninterrupted work, asthenopia and musculoskeletal p...

Leica DM750 P Manual 11 Information for the person responsible for the instrument Information for the person responsible for the instrument Ϙ Ensure that the Leica DM750 P microscope is used only by qualified personnel. Ϙ Ensure that this user manual is always avail-able at the Leica DM750 P microsc...

Leica DM750 P Manual 12 Care instructions General instructions Ϙ Protect the Leica DM750 P microscope against damp, vapors, acids, alkalis, and corrosive substances. Do not store chemi-cals in the vicinity. Ϙ Protect the Leica DM750 P microscope from oil and grease. Never grease or oil mechani-cal p...

Leica DM750 P Manual 13 Accessories, maintenance and repair Accessories Only the following accessories may be used with the Leica DM750 P microscope: Ϙ The Leica accessories described in this user manual. Ϙ Other accessories, provided that these have been expressly approved by Leica as being technic...

Leica DM750 P Manual 14 Electrical data and ambient conditions Environment Temperature for use +10 °C … +40 °C Storage temperature -20 °C … +52 °C +50 °F … +104 °F Manipulation shock 25 mm on 50 mm hard wood Transport shock (unpacked) 100 g / 6 ms Transport shock (packed) 800 mm free fall Transport ...

Leica DM750 P Manual 16 Thank you for purchasing the Leica DM750  P Polarizing Microscope System from Leica Microsystems. The Leica DM750 P is a highly adaptable instrument with superior optics and precision engineering for educational and basic industrial polarized light microscopy applica-tions. I...

Leica DM750 P Manual 17 Ϙ Carefully remove the microscope and any components from the packing carton. Ϙ Check the components against the planned configuration. Ϙ Optional items such as contrast accessories, camera adapters, cameras, and carrying cases are not shipped as part of the stan-dard equipme...

Leica DM750 P Manual 19 2. Align the shape of the module with the shape of the microscope stand. 3. Insert the dovetail of the module into the stand. (Analyzer/Bertrand Lens Module shown). Attaching Analyzer Module or Analyzer/Bertrand Lens Module Tools used Ϙ Allen key 1. Slightly unscrew the set s...

Leica DM750 P Manual 20 Viewing Tube 1. Slightly unscrew the set screw located on the top of the Analyzer module. 2. Align the shape of the polarizing viewing tube with the shape of the module. 3. Insert the dovetail of the polarizing view- ing tube into the module and tighten the set screw firmly w...

Leica DM750 P Manual 21 1. As you rotate the nosepiece clockwise, attach the objectives by screwing them into the nosepiece holes starting with the lowest magnification and advancing to the highest magnification. Installing Objectives   If you have purchased a standard micro-scope configuration, you...

Leica DM750 P Manual 25 Basic setup   As a safety precaution, the power cord has been grounded to the microscope base. Never use an adapter between the power cord and the power source; it will render the grounding feature ineffective. Work Surface •  Always use your microscope on a hard, stable surf...

Leica DM750 P Manual 26   The condenser is furnished with an iris diaphragm, which can be adjusted to match the effective numerical aperture of the objective. 1. To open and close this diaphragm, simply rotate the knurled condenser ring to the right or to the left so that the line on the rotating ri...

Leica DM750 P Manual 27   The polarizer is located under the substage condenser in a swing in/out mount. Swing it into place and be sure it "clicks" into place. 1. Loosen the polarizer locking thumbscrew, which is located on the right-hand side of the polarizer. 2. Rotate the polarizer to th...

Leica DM750 P Manual 29 Focusing 1. Place a polarized light specimen on the stage. 2. Rotate the 10× objective into working posi- tion. 3. Raise the microscope stage using the coarse adjustment knob until you reach its positive stop or the specimen touches the objective. 4. Look into the eyepieces a...

Leica DM750 P Manual 30 Adjust the eyetubes for your interpupillary distance. 1. Fold or unfold the eyetubes until you see one illuminated circle.   The viewing tubes maintain a constant tube length for all interpupillary settings. This means that a change of interpupillary distance does not affect ...

Leica DM750 P Manual 31 Koehler Set up 1. Close the Koehler field diaphragm on the base of the microscope so that its iris leaves are present within the field of view when looking into the eyepieces. 2. Use the condenser focus knob on the left-hand side of the substage to bring the leaves of the fie...

Leica DM750 P Manual 32 Tools used Ϙ Centering keys 1. Retrieve the two centering keys from the packaging and insert the centering tools into the centering holes located above the objective in use. Engage the ball end of the centering tools into the set screws, which are hidden in the centering hole...

Leica DM750 P Manual 34   If you have an Analyzer/Bertrand Lens module, you can view a conoscopic image through the eyepieces. 1. Bring the portion of the specimen you would like to view in conoscopy (with the Bertrand Lens) to the center of the field 2. Swing in the Bertrand Lens by flipping the up...

Leica DM750 P Manual 35   The Leica DM 750 P is equipped with a time delay shutoff capability, which automatically turns the illumination off after 2 hours of no changes in the intensity control. Ϙ The time delay shutoff IS enabled as a default. Changing the status of the time delay shutoff 1. Rotat...

Leica DM750 P Manual 39 General maintenance General •  Always carry the microscope using two hands. There is a handle on the back of the microscope and an undercut in the front for this purpose. Ϙ The cord wrap allows you to wrap the cord in such a way that only the length you need is extended. Ϙ Pr...

Leica DM750 Manual 6 Safety concept The individual modules of the Leica DM microscopy series include an interactive CD-ROM with all relevant user manuals in several languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download...

Leica DM750 Manual 7 Symbols used in this instruction manual Warning of a danger   This symbol indicates especially impor-tant information that is mandatory to read and observe. Failure to comply can cause the following: Ϙ Hazards to personnel Ϙ Functional disturbances or damaged inst-ruments Warnin...

Leica DM750 Manual 8 Important Notes Description The Leica DM750 microscope meets today's state of the art of technology. Nevertheless, hazards may still arise during operation. The potential risks are described below.   Before installing, operating or using the instrument, it is mandatory to read t...

Leica DM750 Manual 9   The Leica DM750 microscope, for which this instruction manual has been writ- ten, is intended for biological routine and research applications. This includes exami-ning specimens taken from the human body for the purpose of gaining information about physiological or pathologic...

Leica DM750 Manual 10 Instructions on use   The Leica DM750 microscope may be used only in closed rooms and must be placed on a solid substrate. The Leica DM750 microscope can be used in clean rooms without any problems.   Always position the Leica DM750 microscope so that you can disconnect it from...

Leica DM750 Manual 12 Health risks and dangers of use Health risks   Workplaces with microscopes facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Depending on the duration of uninterrupted work, asthenopia and musculoskeletal pro...

Leica DM750 Manual 13 Information for the person responsible for the instrument Information for the person responsible for the instrument Ϙ Ensure that the Leica DM750 microscope is used only by qualified personnel. Ϙ Ensure that this user manual is always available at the Leica DM750 microscope. Ϙ ...

Leica DM750 Manual 14 Care instructions General instructions Ϙ Protect the Leica DM750 microscope against damp, vapors, acids, alkalis, and corrosive substances. Do not store chemi-cals in the vicinity. Ϙ Protect the Leica DM750 microscope from oil and grease. Never grease or oil mechani-cal parts o...

Leica DM750 Manual 15 Accessories, maintenance and repair Accessories Only the following accessories may be used with the Leica DM750 microscope: Ϙ The Leica accessories described in this user manual. Ϙ Other accessories, provided that these have been expressly approved by Leica as being technically...

Leica DM750 Manual 16 Electrical data and ambient conditions   In order to maintain this condition and to ensure safe operation, the user must follow the instructions and warnings contained in this instruction manual. Environment Temperature for use +10 °C … +40 °C Storage temperature -20 °C … +52 °...

Leica DM750 Manual 18 Thank you for purchasing the Leica DM750 Compound Microscope from Leica Microsys-tems. This model’s exclusive design features and full range of accessories make it a truly versatile, high quality-instrument for educatio-nal and routine clinical applications.   The instrument is...

Leica DM750 Manual 19 Ϙ Carefully remove the microscope and any components from the packing carton. Ϙ Verify that all components are intact. Ϙ Check the components against the planned configuration. Ϙ Optional items such as contrast accessories, camera adapters, cameras, and carrying cases are not s...

Leica DM750 Manual 21 Substage Illumination   The Leica DM750 is available with two types of Substage Illumination. Identify which type of Illumination you have, as this will be important to know later. Type 1: Standard illumination Adjustable condenser centering with provided tool. Type 2: Koehler ...

Leica DM750 Manual 22 Tools used Ϙ Allen key   There are two types of viewing tube. Identify which viewing tube you have: Type 1: Leica EZ Viewing Tube with integrated eyepieces. Type 2: Standard viewing tube with separate eyepieces 1. Loosen the setscrew (on top of the stand) using the Allen key pr...

Leica DM750 Manual 23 2. Reinstall the Leica EZ Viewing Tube onto the stand. 3. Tighten the captive wingscrew using the wrench included in the delivery package. Leica EZ Viewing Tube – integrated eyepieces   To rotate the Leica EZ viewing tube, either loosen the set screw on the stand or replace the...

Leica DM750 Manual 25 Leica Standard Viewing Tube – separated eyepieces 2. Secure the eyepieces in the tubes by tighte- ning the silver screws at the bottom of the tubes using a standard Phillips screwdriver (not included in the delivery package).   The eyepieces will still rotate, but they will be ...

Leica DM750 Manual 26   If you wear eyeglasses for microscope viewing, keep the rubber eyecups folded down. If you do not wear eyeglasses, you may find it useful to unfold the rubber eyecups in order to help block out ambient room light. Eyecups   If you have purchased a standard microscope configur...

Leica DM750 Manual 28 Installing the Substage Condenser Substage Condenser   The Leica DM750 has an open substage condenser mount, therefore the conden- ser needs to be installed. 1. Move the specimen stage upwards as far as it will go by using the coarse focusing knob on the side of the microscope....

Leica DM750 Manual 31 Work Surface   Always use your microscope on a hard, stable surface. Power Cord   If the power cord is not already atta-ched, attach it securely to the back of the microscope. Setting the Illumination Intensity   Set the illumination to the lowest setting to start with, using t...

Leica DM750 Manual 32   If you have purchased a standard Leica DM750 configuration, the condenser has already been pre-centered by Leica Microsys-tems. 1. Open the condenser aperture by rotating the knurled ring on the condenser to the right.   Make sure that the condenser is in the highest position...

Leica DM750 Manual 33 Using the Condenser   The condenser is furnished with an iris diaphragm, which can be adjusted to match the effective numerical aperture of the objective 1. To open and close this diaphragm, simply turn the knurled ring on the condenser to the right or left so that the line on ...

Leica DM750 Manual 34 1. Position a specimen slide on the specimen stage by sliding it under the slide grips.   Slide grips hold the slide in place. 2. Using the X/Y-stage control, position the specimen slide such that a part of the speci-men is under the objective used. Prepare to View a Specimen S...

Leica DM750 Manual 35 1. Rotate the objective nosepiece (using the knurled ring) in such a way that the objec-tive with the lowest magnification level is rotated into the working position. 2. Move the specimen stage upwards by turning the coarse focusing knob as far as it will go to the maximum posi...

Leica DM750 Manual 36 Adjust the eyetubes 1. Adjust the tubes to your interpupillary distance. Fold or unfold the eyetubes to decrease or increase the distance between the eyepieces until you see one illuminated circle.   The viewing tubes maintain a constant tube length for all interpupillary setti...

Leica DM750 Manual 38   If your Leica DM750 is fitted with a field diaphragm for Koehler Illu-mination, use the following procedure to assure condenser cente- ring and focus. 1. Connect the Koehler field diaphragm to the base of the microscope so that the diaphragm leaves are in the field of view wh...

Leica DM750 Manual 40 1. Search for the area on the specimen slide that you want to study. 2. Move the specimen stage down to the lowest position using the coarse adjust-ment knob. 3. Add a drop of immersion oil from Leica to the area of the specimen slide you are studying. 4. Rotate the oil immersi...

Leica DM750 Manual 42   The Leica DM750 is equipped with a time delay shutoff capability, which automati- cally turns the illumination off after 2 hours of no changes in the intensity control. Ϙ All four-position nosepiece stands have the time delay shutoff enabled as a default (mostly educational a...

Leica DM750 Manual 46 General maintenance Storage and handling   Always use both hands to carry the microscope. There is a handle on the back of the microscope and an undercut in the front for this purpose. Ϙ The cord wrap allows you to wrap the cord in such a way that only the length you need is ex...