Page 3 - Table of Contents
Table of Contents Chapter One: Introduction.................................. 1 Chapter Overview .................................................................. 1 Introduction ........................................................................... 2 Style Reference ..............................
Page 5 - Chapter Overview
Leica CW4000 Quick Start Guide Page 1 Chapter One: Introduction Chapter Overview This chapter contains the following topics: ♦ Introduction ♦ Style Reference ♦ Welcome ♦ What is Leica CW4000? General Leica CW4000 Karyo Leica CW4000 FISH Leica CW4000 CGH Leica CW4000 MFISH Leica CW4000 Satellite Capt...
Page 6 - Introduction; Installation; Configuration
Page 2 Leica CW4000 Quick Start Guide Introduction This document describes the V1.0 release of the Leica CW4000 Cytogenetics Application Solutions CD. You should read this document before installing your copy of this CD. This document is intended to guide you through the installation process, setup ...
Page 7 - Style Reference; FILE
Leica CW4000 Quick Start Guide Page 3 Style Reference The following conventions have been used to make this manual easier to read: Menus FILE (bold, capitals). Menu Commands/ Buttons/ Dialogs Open, OK (bold). References Chapter 1 (italics). Notes, Tips, Warnings, References and Instructions Appear a...
Page 8 - Welcome; Leica CW4000 Cytogenetics Image Analysis
Page 4 Leica CW4000 Quick Start Guide Welcome Welcome to your new Leica CW4000 Cytogenetics Image Analysis Workstation . We at Leica Microsystems are sure that you will be pleased with your new system. We hope that you find the software both intuitive and easy to use. To help with this we have re -o...
Page 9 - Leica; General; Leica CW4000 Automated Karyotyping Module
Leica CW4000 Quick Start Guide Page 5 What is Leica CW4000? The Leica CW4000 total imaging solution combines a number of Cytogenetics imaging techniques, with Leica microscopes and cameras, to offer you a flexible cytogenetics imaging solution that suits your laboratory needs. Leica CW4000 comes in ...
Page 10 - Karyotyping Features
Page 6 Leica CW4000 Quick Start Guide Karyotyping Features ♦ Dynamic presentation of separation and overlap cuts in metaphase editing – saves time ♦ Camera control in live window – saves time while providing optimal camera adjustment ♦ Automatic include function ♦ R banded ideograms ♦ Accurate displ...
Page 11 - CGH Features
Leica CW4000 Quick Start Guide Page 7 CGH Features ♦ Unique “two -scale” CGH gain and loss bars. ♦ Improved correction of centromere position. ♦ Improved CGH midline correction of chromosomes. ♦ Editable CGH gain and loss bars - better presentation of data. ♦ Select individual profiles – improved an...
Page 12 - Leica CW4000 Satellite Capture; Minimum System Requirements
Page 8 Leica CW4000 Quick Start Guide Leica CW4000 Satellite Capture The Leica CW4000 Satellite Capture module is a complete network solution designed specifically for cytogenetics image acquisition and data entry in network configurations . The module allows you to acquire metaphase images for succ...
Page 13 - Software Requirements
Leica CW4000 Quick Start Guide Page 9 Software Requirements Note : A back-up device and a back-up strategy for images and data are essential. The user must ensure that regular and reliable back-up of important files is performed. For Leica Software to function correctly the following Software is req...
Page 17 - Installation Procedure; Then you
Leica CW4000 Quick Start Guide Page 13 Installation Procedure If you have already installed some of the Leica Cytogenetics Applications, we recommend that you uninstall them before reinstalling this soft ware. Refer to: the un-installation instructions on page 15. Note : if you do not uninstall, you...
Page 22 - Configuring Your System
Page 18 Leica CW4000 Quick Start Guide Configuring Your System Again, if you have just received a new system the appropriate software will have been installed and configured. Please do not change this configuration. If you have installed this software yourself, you may need to tell the software what...
Page 23 - Microscope Settings; Saving Settings
Leica CW4000 Quick Start Guide Page 19 Microscope Settings If you have any Microscope automation (e.g. motorised X/Y Stage, or Focus), you should click on the ‘Microscope Settings…’ button and then select the items that you require. You can click ‘Test’ to verify these settings before closing the di...
Page 26 - Run the Leica Cytogenetics Applications
Page 22 Leica CW4000 Quick Start Guide Introduction As Leica CW4000 uses the conventions of Microsoft Windows 2000 , it is a good idea to familiarise yourself with the basic operations of the Microsoft Windows 2000 user interface before you begin. Starting the Applications You can run the Leica ...
Page 27 - The Leica CW4000 Karyotyping Desktop; Main Menu Bar; Main Toolbar
Leica CW4000 Quick Start Guide Page 23 The Leica CW4000 Karyotyping Desktop The Leica CW4000 Karyotyping Desktop appears as show below: The Main Menu Bar provides access to all the features. The Main Toolbar provides shortcuts to the most frequently used features.
Page 28 - The Main CW4000 Karyo Menus
Page 24 Leica CW4000 Quick Start Guide The Main CW4000 Karyo Menus The main Leica Karyo CW4000 menus are described below: FILE The FILE menu contains commands for: ♦ Managing files. ♦ Logging on and off. ♦ Printing. ♦ Exiting. EDIT The EDIT menu allows you to: ♦ Undo actions. ♦ Copy and paste images...
Page 29 - The Main CW4000 FISH Menus
Leica CW4000 Quick Start Guide Page 25 The Main CW4000 FISH Menus The main Leica CW4000 FISH menus are described below: FILE The FILE menu contains commands for: ♦ Managing files. ♦ Logging on and off. ♦ Printing. ♦ Exiting. EDIT The EDIT menu allows you to: ♦ Undo actions. ♦ Copy and paste images. ...
Page 30 - The Main CW4000 Karyo Toolbar
Page 26 Leica CW4000 Quick Start Guide The Main CW4000 Karyo Toolbar The Leica CW4000 Karyo main toolbar provides a quick way of accessing commonly used functions. The functions of the buttons on this toolbar are described below: New Window — Opens a new image window. Live — Displays the Camera Live...
Page 31 - Add Result; Review Result
Leica CW4000 Quick Start Guide Page 27 Add Result — Allows the addition of a new result to Cybase. Review Result — Allows import of metaphase and karyogram from Cybase for review purposes. Note: You can customise the main toolbar so that it contains the options you use most frequently.
Page 32 - The Main CW4000 FISH Toolbar
Page 28 Leica CW4000 Quick Start Guide The Main CW4000 FISH Toolbar The Leica CW4000 FISH main toolbar provides a quick way of accessing commonly used functions. The functions of the buttons on this toolbar are described below: New Window — Opens a new image window. Open Image — Opens an image file ...
Page 33 - Acquire All Probes; Make FISH Image; Add Record to Archive; Import record from Archive; Print Archive report
Leica CW4000 Quick Start Guide Page 29 Acquire All Probes — Acquisition of all specified probes. Make FISH Image — Produces merged FISH image. Add Record to Archive — Adds current record to FISH database. Import record from Archive — Allows record to be recalled from FISH database. Print Archive rep...
Page 34 - Obtaining Leica CW4000 Help
Page 30 Leica CW4000 Quick Start Guide Obtaining Leica CW4000 Help The Leica CW4000 Cytogenetics Workstation is fully supported by on-line help. Clicking the HELP menu will start Adobe Acrobat Reader and the Leica CW4000 User Manual (CW4000help.pdf) . Both Adobe Acrobat and the Leica CW4000 User Man...