Leica CW4000 Quick Start Guide- User Manual

Leica CW4000 Quick Start Guide Page 3 Style Reference The following conventions have been used to make this manual easier to read: Menus FILE (bold, capitals). Menu Commands/ Buttons/ Dialogs Open, OK (bold). References Chapter 1 (italics). Notes, Tips, Warnings, References and Instructions Appear a...

Page 4 Leica CW4000 Quick Start Guide Welcome Welcome to your new Leica CW4000 Cytogenetics Image Analysis Workstation . We at Leica Microsystems are sure that you will be pleased with your new system. We hope that you find the software both intuitive and easy to use. To help with this we have re -o...

Leica CW4000 Quick Start Guide Page 5 What is Leica CW4000? The Leica CW4000 total imaging solution combines a number of Cytogenetics imaging techniques, with Leica microscopes and cameras, to offer you a flexible cytogenetics imaging solution that suits your laboratory needs. Leica CW4000 comes in ...

Page 6 Leica CW4000 Quick Start Guide Karyotyping Features ♦ Dynamic presentation of separation and overlap cuts in metaphase editing – saves time ♦ Camera control in live window – saves time while providing optimal camera adjustment ♦ Automatic include function ♦ R banded ideograms ♦ Accurate displ...

Leica CW4000 Quick Start Guide Page 7 CGH Features ♦ Unique “two -scale” CGH gain and loss bars. ♦ Improved correction of centromere position. ♦ Improved CGH midline correction of chromosomes. ♦ Editable CGH gain and loss bars - better presentation of data. ♦ Select individual profiles – improved an...

Page 8 Leica CW4000 Quick Start Guide Leica CW4000 Satellite Capture The Leica CW4000 Satellite Capture module is a complete network solution designed specifically for cytogenetics image acquisition and data entry in network configurations . The module allows you to acquire metaphase images for succ...

Leica CW4000 Quick Start Guide Page 9 Software Requirements Note : A back-up device and a back-up strategy for images and data are essential. The user must ensure that regular and reliable back-up of important files is performed. For Leica Software to function correctly the following Software is req...

Leica CW4000 Quick Start Guide Page 13 Installation Procedure If you have already installed some of the Leica Cytogenetics Applications, we recommend that you uninstall them before reinstalling this soft ware. Refer to: the un-installation instructions on page 15. Note : if you do not uninstall, you...

Page 18 Leica CW4000 Quick Start Guide Configuring Your System Again, if you have just received a new system the appropriate software will have been installed and configured. Please do not change this configuration. If you have installed this software yourself, you may need to tell the software what...

Leica CW4000 Quick Start Guide Page 19 Microscope Settings If you have any Microscope automation (e.g. motorised X/Y Stage, or Focus), you should click on the ‘Microscope Settings…’ button and then select the items that you require. You can click ‘Test’ to verify these settings before closing the di...

Page 22 Leica CW4000 Quick Start Guide Introduction As Leica CW4000 uses the conventions of Microsoft Windows 2000  , it is a good idea to familiarise yourself with the basic operations of the Microsoft Windows 2000  user interface before you begin. Starting the Applications You can run the Leica ...

Leica CW4000 Quick Start Guide Page 23 The Leica CW4000 Karyotyping Desktop The Leica CW4000 Karyotyping Desktop appears as show below: The Main Menu Bar provides access to all the features. The Main Toolbar provides shortcuts to the most frequently used features.

Page 24 Leica CW4000 Quick Start Guide The Main CW4000 Karyo Menus The main Leica Karyo CW4000 menus are described below: FILE The FILE menu contains commands for: ♦ Managing files. ♦ Logging on and off. ♦ Printing. ♦ Exiting. EDIT The EDIT menu allows you to: ♦ Undo actions. ♦ Copy and paste images...

Leica CW4000 Quick Start Guide Page 25 The Main CW4000 FISH Menus The main Leica CW4000 FISH menus are described below: FILE The FILE menu contains commands for: ♦ Managing files. ♦ Logging on and off. ♦ Printing. ♦ Exiting. EDIT The EDIT menu allows you to: ♦ Undo actions. ♦ Copy and paste images. ...

Page 26 Leica CW4000 Quick Start Guide The Main CW4000 Karyo Toolbar The Leica CW4000 Karyo main toolbar provides a quick way of accessing commonly used functions. The functions of the buttons on this toolbar are described below: New Window — Opens a new image window. Live — Displays the Camera Live...

Leica CW4000 Quick Start Guide Page 27 Add Result — Allows the addition of a new result to Cybase. Review Result — Allows import of metaphase and karyogram from Cybase for review purposes. Note: You can customise the main toolbar so that it contains the options you use most frequently.

Page 28 Leica CW4000 Quick Start Guide The Main CW4000 FISH Toolbar The Leica CW4000 FISH main toolbar provides a quick way of accessing commonly used functions. The functions of the buttons on this toolbar are described below: New Window — Opens a new image window. Open Image — Opens an image file ...

Leica CW4000 Quick Start Guide Page 29 Acquire All Probes — Acquisition of all specified probes. Make FISH Image — Produces merged FISH image. Add Record to Archive — Adds current record to FISH database. Import record from Archive — Allows record to be recalled from FISH database. Print Archive rep...

Page 30 Leica CW4000 Quick Start Guide Obtaining Leica CW4000 Help The Leica CW4000 Cytogenetics Workstation is fully supported by on-line help. Clicking the HELP menu will start Adobe Acrobat Reader and the Leica CW4000 User Manual (CW4000help.pdf) . Both Adobe Acrobat and the Leica CW4000 User Man...