Leica ASP200 S- Manuals

7 Leica ASP200 S 1.1 Symbols in the text and their meanings (continued) 1. Important Information SN REF Serial number Order No. Observe the Instructions for Use The package contents are fragile and must be handled with care. Indicates the correct upright position of the package. The package must be ...

11 Leica ASP200 S 2. Safety Warnings – Operating the instrument The instrument may only be operated by trained laboratory personnel. It must only be operat- ed for the purpose of its designated use and according to the instructions contained in these Instructions for Use. In an emergency, the instru...

12 Instructions for Use V 1.4, RevE – 03/2013 2. Safety Warnings – Handling reagents Warnings – Cleaning and maintenance Take care when handling solvents! Always wear rubber gloves and safety goggles when handling the chemicals used in this instrument. Reagents used for tissue infiltration can be bo...

13 Leica ASP200 S 2.3 Safety features on the instrument The Leica ASP200 tissue processor is equipped with numerous safety func- tions and with sophisticated software control mechanisms. These ensure that the specimens remain undamaged in the event of a power failure or other malfunction during the ...

14 Instructions for Use V 1.4, RevE – 03/2013 3.1 Overview – instrument components 3. Instrument Components and Specifications 2 3 4 5 13 6 7 14 15 16 17 18 19 20 21 22 12 11 8 10 9 1 Fig. 2 24 23

15 Leica ASP200 S Overview – instrument components 1 - Basic instrument – processor module2 - Basic instrument – reagent module3 - Wax bath lids4 - Retort lid5 - Monitor6 - Cover flap of the instrument console7 - Instrument console with:8 - Printer port9 - Local alarm connection10 - Remote alarm con...

16 Instructions for Use V 1.4, RevE – 03/2013 3. Instrument Components and Specifications 3.2 Specific instrument options • Optionally usable R eagent M anagement S ystem (RMS), displays service life and usage fre- quency of the individual reagents and enables automatic definition of the reagent seq...

17 Leica ASP200 S 3. Instrument Components and Specifications 3.3 Standard delivery - packing list To prevent damage to the instrument or specimens, only accessories and spare parts autho- rized by Leica may be used. The standard equipment of the Leica ASP200 S includes the following parts: Part No....

18 Instructions for Use V 1.4, RevE – 03/2013 3. Instrument Components and Specifications Please check all delivered parts against the packing list and against your order to verify whether the delivery is complete! Should you find any discrepancies, please contact your Lei- ca sales office without d...

19 Leica ASP200 S 3. Instrument Components and Specifications Nominal supply voltages: Two factory-preset voltages (not user-adjustable):100 to 120 V or 230 to 240 V Nominal frequency: 50 to 60 Hz Main fuses: 2 melting fuses, 20 x 5 mm, UL-approved• for 100 to 120 V F 10 A 250 VAC• for 230 to 240 V ...

20 Instructions for Use V 1.4, RevE – 03/2013 3. Instrument Components and Specifications Retort Capacity: max. 200 cassettes Reagent volume: 3.5 l with volume replacement body Temperature (paraffin): 40 to 65 °C Temperature (processing reagents): Ambient temperature or 35 to 55 °C Temperature (clea...

21 Leica ASP200 S 3.5 Compatible reagents 3. Instrument Components and Specifications Hardware and Software: • Large color LCD touchscreen.• User-friendly, intelligent software.• 3.5" disk drive and printer port.• Alarm system with two remote alarm sockets.• Password-protected instrument supervi...

22 Instructions for Use V 1.4, RevE – 03/2013 3. Instrument Components and Specifications The following reagents may be used in the Leica ASP200 S: Fixatives Dehydration Clearing Paraffinizing 1st Formalin (buffered or unbuffered) 1st Ethanol 1st Xylene 1st Wax 2nd Isopropanol 2nd Toluene* 3. Methan...

23 Leica ASP200 S 3.6.1 Cycle for changing reagents Reagent Week 1 Week 2 Week 3 Week 4 Formalin At least 3 times a week At least 3 times a week At least 3 times a week At least 3 times a week Formalin At least 3 times a week At least 3 times a week At least 3 times a week At least 3 times a week 70...

24 Instructions for Use V 1.4, RevE – 03/2013 4. Initial Operation 4.1.1 Moving the instrument After unpacking the instrument (see Unpack- ing instructions on the outside of the transport crate), handle it only by the handles marked with ‘ ‘ to move it to its final location. When operating the instr...

25 Leica ASP200 S 4. Instrument Setup 4.2 Electrical connection • Check the voltage label ( Fig. 3 ) on the rear of the instrument to ensure that the instrument delivered is set to the correct voltage range. Fig. 3 Severe damage may occur if the in- strument is connected to a power sup- ply voltage ...

27 Leica ASP200 S 4. Instrument Setup Fig. 5 • Move the instrument to its final setup location. Active carbon filter • Unpack the active carbon filter ( 13 ) and insert it (see Fig. 5 ). When doing so, make sure that the filter is inserted with the correct side up.Note the direction of the label ( 2...

28 Instructions for Use V 1.4, RevE – 03/2013 Fig. 8 Inserting the volume replacement body 26 25 27 25 19 Important! The instrument must be operated only when the volume replacement body is in the retort (even during cleaning pro- grams). Otherwise, malfunctions may cause damage to the instrument. •...

29 Leica ASP200 S 4. Instrument Setup Fig. 9 Printer (optional) • Swing the door of the instrument console ( 6 ) upwards. • Connect the printer to the printer port ( 8 ) using a Centronics standard cable. ( Fig. 9 ) • Your Leica dealer can recommend a suitable printer. • If compliance with the elect...

30 Instructions for Use V 1.4, RevE – 03/2013 4. Initial Operation Changing the clip • Once the clip is installed, the handle ( 4 ) of the speci- men basket cannot be removed, as otherwise the clip will be bent. • To remove or replace a clip, all parts that are inside the basket (e.g. spiral insert ...

31 Leica ASP200 S 4. Instrument Setup Remote alarm This alarm is also external to the Leica ASP200 S. If installed, it might typically be connected to a remote dialer that sends an automated phone message to the person responsible for after- hours problems. The remote alarm is only generated when th...

32 Instructions for Use V 1.4, RevE – 03/2013 4.7 Switching the instrument on 4. Initial Operation • After being switched on, the instrument will take a few minutes to initialize. See opposite for cor- responding touchscreen display ( Fig. 14 ). • If the retort is not locked, a signal tone sounds fo...

33 Leica ASP200 S SYSTEM DIAGNOSTICS menu If TOUCH HERE ... is touched during initialization ( Fig. 14 ) and the pass- word for supervisor mode is en- tered, the SYSTEM DIAGNOSTICS menu ( Fig. 16 ) opens. This menu provides access to basic instru- ment settings. Notice!Only experienced operators may...

34 Instructions for Use V 1.4, RevE – 03/2013 4.8 Touchscreen functions The Leica ASP200 S is programmed and operated via a color LCD touchscreen.The instrument's control software contains an online help feature including detailed information about all screen func- tions and error messages. The soft...

35 Leica ASP200 S 4. Instrument Setup 4.9 Checklist for initial operation Parameter selection Set warning values for the age of the carbon filter and the operating hours of the pressure air pump.Enter the instrument name and select the lan- guage. Optionally, set up the printer. Here, configure all ...

36 Instructions for Use V 1.4, RevE – 03/2013 4.10 Switching off the instrument If the instrument must be completely switched off or disconnected from the power supply, please proceed as follows: • Press MENU to switch to the MENU FUNCTIONS window. • Press the EXIT APPLICATION button there. A messag...

37 Leica ASP200 S 5. Operation 5.1 Setting up the instrument parameters The SYSTEM SETUP screen is divided up into five selection fields: • Program options • Display/Buzzer • Date/time • Security • Instrument Fig. 24 5.1.1 System setup In the MORE MENU FUNCTIONS press SYSTEM SETUP Fig. 22 From the s...

38 Instructions for Use V 1.4, RevE – 03/2013 PROGRAM OPTIONS Select the options with which programs are executed here. Press a button to change the associated value.• STATION SEQUENCE : By Age or Sequential By Age - RMS is activated, reagents will automatically be used in order of increasing cleanl...

39 Leica ASP200 S INSTRUMENT Press PARAFFIN BATH SET TEMP. to open the input screen ( Fig. 30 ) for the wax bath temperature. Set the temperature according to the value required by the paraffin used. Select the highest permissible temperature for the paraffin to ensure that a minimum loss of tempera...

40 Instructions for Use V 1.4, RevE – 03/2013 SECURITY Access rights to the instrument are managed here as user profiles . 5. Operation SUPERVISOR MODE : Enabled or Disabled Enabled - There are two different user profiles. A password is required for supervisor level access to the in- strument. Enabl...

46 Instructions for Use V 1.4, RevE – 03/2013 Entering / modifying reagent thresholds If warning thresholds are required for a certain reagent, enter them as fol- lows:• Highlight the reagent to be modified, either by pressing the reagent name or using the UP/DOWN buttons. • Press the header of the ...

50 Instructions for Use V 1.4, RevE – 03/2013 5. Operation Editing program steps • To edit a step, highlight the corresponding line and press the respective headline. • In the entry windows that pop up, enter / select the program step settings. ↓ ↓ ↓ ↓ ↓ Fig. 47

51 Leica ASP200 S Editing program steps (continued) Setting a delay step A delay step is a step that is extended to ensure that a program will finish at a specified time.• Highlight the program step you wish to define as delay step.• Touch the DELAY button. The delay symbol is moved to the selected ...

53 Leica ASP200 S Assigning a day for the end time If an end time has been assigned to a Favorite program, a specific weekday can also be set on which the program is to end.• Press the DAY button. • Select the desired day from the list and confirm with OK . Assigning a symbol to a "Favorite"...

54 Instructions for Use V 1.4, RevE – 03/2013 5.1.8 Configuring the stations Adding/changing a reagent Select the desired station. Either by pressing the reagent name or using the UP/DOWN buttons.• Touch the REAGENT NAME button. • The SELECT REAGENT field appears. This window ( Fig. 52 ) shows a lis...

55 Leica ASP200 S 5.1.9 Reagent groups Fig. 53 Changing the color of a reagent group • Highlight the line containing the reagent to be modified.• Press the COLOR button in the table header; the SELECT COLOR window appears. • Select the color you want and confirm with OK . • To exit the display witho...

58 Instructions for Use V 1.4, RevE – 03/2013 5. Operation 5.2.2 Replacing the paraffin Draining the paraffin • Connect the paraffin drain hose ( 36 ) to the spout ( 17 ) of the paraffin drain at the front of the instrument ( Fig. 57 ) and into a catch tank. Fig. 57 • Select the paraffin reservoir t...

59 Leica ASP200 S Filling with paraffin pellets 5. Operation Fig. 60 • Fill the paraffin reservoir to the brim ( Fig. 60 , 1 ) with paraffin pellets. This corresponds to ap- prox. 3.5 kg/bath (Leica Histowax pellets). • Press BACK and REAGENT STATUS to switch to the status table ( Fig. 59 ). Select ...

60 Instructions for Use V 1.4, RevE – 03/2013 Filling with liquid paraffin Software control If the instrument has been filled with liquid paraffin, the instrument's internal calculation of the melting time can be disabled. To do so, in supervisor mode, open the SERVICE FUNCTIONS ( Fig. 61 ) window. ...

61 Leica ASP200 S 5.3 Running programs There are two different windows from which a program can be started: In the Start screen, press the ALL PROGRAMS button. This window displays all of the infiltration programs defined in the instrument. Up to five favorites can be configured in the ASP200 S. A &...

62 Instructions for Use V 1.4, RevE – 03/2013 5.3.1 Editing a program that has been called up. Fig. 65 To modify a program before the start, press the EDIT button. A message appears that the changes will affect running programs only. After confirming with YES , the program edit window appears ( Fig....

63 Leica ASP200 S The RUN OPTIONS function displays the options with which the current program is started. • LEVEL TEST enabled: The lower level sensor in the retort is acti- vated. The program is stopped with an error message if the level is not reached. • WAVE MOTION activated: During each infiltr...

65 Leica ASP200 S 5. Operation 5.3.3 Opening the retort during a program interruption Fig. 72 Fig. 73 Fig. 71 • After the signal tone stops, turn the lever all the way to the right ( Fig. 73 ) and open the retort lid.  • To unlock the retort, turn the lever for locking/unlocking ( Fig. 71 ) approxi...

66 Instructions for Use V 1.4, RevE – 03/2013 5. Operation 5.3.4 Retort emergency release (continued) Fig. 74  • Turn the retort lock further clockwise using the sin- gle-head wrench ( Fig. 75 , 3 ) and overcome the stop (breaking the retaining pin at the predetermined breaking point). 1 Ensure tha...

70 Instructions for Use V 1.4, RevE – 03/2013 6. Troubleshooting 6.1 General → For all error states, the instrument takes corrective measures to protect the specimens, except in such cases where continued functioning of the instrument would be possible only under hazard- ous operating conditions. Th...

71 Leica ASP200 S 6. Troubleshooting 6.3 Troubleshooting → Use the SMART SCREEN functions to individually test operations such as fill, drain, pressurizing and evacuation operations. If you cannot identify the problem by carrying out the above listed steps, call Leica Technical Service. If a problem...

72 Instructions for Use V 1.4, RevE – 03/2013 6. Troubleshooting 6.4 Typical fill or drain problems Fill and drain problems may be due to several causes: 1. There is insufficient reagent • Verify that the fill level of the reagent containers and the paraffin reservoirs is sufficient. 2. Insufficient...

73 Leica ASP200 S 7. Cleaning and Maintenance 7.1 Clean programs The cleaning programs are at the end of the list of all pro- grams ( Fig. 45 ). To display a clean program, highlight the respective line and press EDIT . Fig. 81 → → 7.1.1 Retort cleaning programs A cleaning program comprises a minimu...

74 Instructions for Use V 1.4, RevE – 03/2013 7. Cleaning and Maintenance Editing steps • To edit a program step, highlight the corresponding line and press the respective table header.• To disable a step, set the number of cycles to " 0 ". Only cleaning water steps can be disabled. The foll...

76 Instructions for Use V 1.4, RevE – 03/2013 7. Cleaning and maintenance 7.1.3 Smart clean The smart cleaning prepares the ASP200 S for transport. During a smart clean, a series of air cleaning steps will be applied which will remove any excess liquid (condensate) from all internal tubing.Before st...

77 Leica ASP200 S 7.2 General cleaning steps Paraffin reservoirs 7. Cleaning and Maintenance Regularly inspect the collecting tray ( 16 ) for signs of leaking chemicals ( Fig. 84 ). Checking the drip tray Fig. 84 16 Work carefully! Be careful as the walls of the paraffin reservoirs are very hot and ...

78 Instructions for Use V 1.4, RevE – 03/2013 7. Cleaning and Maintenance Replacing the active carbon filter • The life of the active carbon filter will depend on the reagent types used and the frequency of vacuum cycles. • The filter ( 13 ) should be replaced at least every 3 months. ( Fig. 86 ) Af...

79 Leica ASP200 S Cleaning the retort lid • Remove wax from the inside of the retort lid with the plastic scraper provided. Thoroughly remove all wax deposits from around the lid seal. • For convenience, the lid may be removed during cleaning. Lift the lid to a vertical position, release the hinge l...

80 Instructions for Use V 1.4, RevE – 03/2013 7. Cleaning and Maintenance Cleaning the surface of the infiltration module Important in order to ensure that the seals always seal properly.• Remove both covers for cleaning. • First, clean the stainless steel surface using the plastic spatulas, then wi...

81 Leica ASP200 S Cleaning the reagent containers • Empty and clean the reagent containers. Using a bottle brush and a laboratory detergent in warm water. 7. Cleaning and Maintenance Never clean reagent containers in an automatic dishwasher. The reagent containers are NOT dishwasher-proof! The reage...

82 Instructions for Use V 1.4, RevE – 03/2013 7. Cleaning and Maintenance Fig. 87 7.2.2 Periodic cleaning and maintenance (continued) 44 Fig. 88 Fig. 89 Use the cleaning tool together with the microfiber cloth ( Fig. 87 ) as shown in Fig. 89 . 45 46 47 The level sensors must be cleaned of all residu...

83 Leica ASP200 S 7. Cleaning and Maintenance 7.3 Checklist for preventive maintenance Step Daily Weekly Monthly every 3 months Lubricate the O-rings of the reagent con- tainer and check them for damage.   * * During re- mote filling and draining After exiting the retort cleaning program, wipe the...

85 Leica ASP200 S 8. Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control proce- dure based on the Leica in-house testing standards, and that the product is faultless and complies with all...

86 Instructions for Use V 1.4, RevE – 03/2013 Appendix 1 Notes on Specimen Preparation • Cut the tissue samples that are to be embedded to a size that fits the type of tissue and embedding cassette being used. • Use a sharp and clean knife in order to prevent the transmission of contaminants and to ...

www.LeicaBiosystems.com Leica Biosystems Nussloch GmbH Heidelberger Straße 17-19D- 69226 NusslochPhone: +49 6224 - 143 0 Fax: +49 6224 - 143 268 Web: www.LeicaBiosystems.com