Page 3 - WARNING
3 Leica ASP200 S The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the presen...
Page 4 - Contents
4 Instructions for Use V 1.4, RevE – 03/2013 Contents 1. Important Information ............................................................................................................... 6 1.1 Symbols in the text and their meanings ...................................................................
Page 6 - Important Information; Symbols in the text and their meanings
6 Instructions for Use V 1.4, RevE – 03/2013 1. Important Information 1.1 Symbols in the text and their meanings (5) ENTER Warnings appear in a gray box and are marked by a warning triangle . Instrument surfaces which become hot during operation are marked with this symbol. Avoid direct contact with...
Page 7 - Important Information; SN
7 Leica ASP200 S 1.1 Symbols in the text and their meanings (continued) 1. Important Information SN REF Serial number Order No. Observe the Instructions for Use The package contents are fragile and must be handled with care. Indicates the correct upright position of the package. The package must be ...
Page 11 - Warnings – Operating the instrument
11 Leica ASP200 S 2. Safety Warnings – Operating the instrument The instrument may only be operated by trained laboratory personnel. It must only be operat- ed for the purpose of its designated use and according to the instructions contained in these Instructions for Use. In an emergency, the instru...
Page 12 - Take care when handling solvents!
12 Instructions for Use V 1.4, RevE – 03/2013 2. Safety Warnings – Handling reagents Warnings – Cleaning and maintenance Take care when handling solvents! Always wear rubber gloves and safety goggles when handling the chemicals used in this instrument. Reagents used for tissue infiltration can be bo...
Page 13 - Safety features on the instrument; Overpressure protection
13 Leica ASP200 S 2.3 Safety features on the instrument The Leica ASP200 tissue processor is equipped with numerous safety func- tions and with sophisticated software control mechanisms. These ensure that the specimens remain undamaged in the event of a power failure or other malfunction during the ...
Page 14 - Overview – instrument components; Instrument Components and Specifications
14 Instructions for Use V 1.4, RevE – 03/2013 3.1 Overview – instrument components 3. Instrument Components and Specifications 2 3 4 5 13 6 7 14 15 16 17 18 19 20 21 22 12 11 8 10 9 1 Fig. 2 24 23
Page 15 - Overview – instrument components; Instrument Components and Specifications; Instrument Components and Accessories
15 Leica ASP200 S Overview – instrument components 1 - Basic instrument – processor module2 - Basic instrument – reagent module3 - Wax bath lids4 - Retort lid5 - Monitor6 - Cover flap of the instrument console7 - Instrument console with:8 - Printer port9 - Local alarm connection10 - Remote alarm con...
Page 16 - Specific instrument options
16 Instructions for Use V 1.4, RevE – 03/2013 3. Instrument Components and Specifications 3.2 Specific instrument options • Optionally usable R eagent M anagement S ystem (RMS), displays service life and usage fre- quency of the individual reagents and enables automatic definition of the reagent seq...
Page 17 - rized by Leica may be used.
17 Leica ASP200 S 3. Instrument Components and Specifications 3.3 Standard delivery - packing list To prevent damage to the instrument or specimens, only accessories and spare parts autho- rized by Leica may be used. The standard equipment of the Leica ASP200 S includes the following parts: Part No....
Page 18 - ca sales office without delay.; Standard delivery – packing list; Cleaning tools for prism
18 Instructions for Use V 1.4, RevE – 03/2013 3. Instrument Components and Specifications Please check all delivered parts against the packing list and against your order to verify whether the delivery is complete! Should you find any discrepancies, please contact your Lei- ca sales office without d...
Page 19 - Nominal supply voltages:; Paraffin reservoirs; Number of containers:
19 Leica ASP200 S 3. Instrument Components and Specifications Nominal supply voltages: Two factory-preset voltages (not user-adjustable):100 to 120 V or 230 to 240 V Nominal frequency: 50 to 60 Hz Main fuses: 2 melting fuses, 20 x 5 mm, UL-approved• for 100 to 120 V F 10 A 250 VAC• for 230 to 240 V ...
Page 20 - Retort
20 Instructions for Use V 1.4, RevE – 03/2013 3. Instrument Components and Specifications Retort Capacity: max. 200 cassettes Reagent volume: 3.5 l with volume replacement body Temperature (paraffin): 40 to 65 °C Temperature (processing reagents): Ambient temperature or 35 to 55 °C Temperature (clea...
Page 21 - Compatible reagents; Hardware and Software:; Use of the ASP200 S is permitted with the reagents specified in
21 Leica ASP200 S 3.5 Compatible reagents 3. Instrument Components and Specifications Hardware and Software: • Large color LCD touchscreen.• User-friendly, intelligent software.• 3.5" disk drive and printer port.• Alarm system with two remote alarm sockets.• Password-protected instrument supervi...
Page 22 - Fixatives; use acetone, benzene or trichlorethane in the instrument.
22 Instructions for Use V 1.4, RevE – 03/2013 3. Instrument Components and Specifications The following reagents may be used in the Leica ASP200 S: Fixatives Dehydration Clearing Paraffinizing 1st Formalin (buffered or unbuffered) 1st Ethanol 1st Xylene 1st Wax 2nd Isopropanol 2nd Toluene* 3. Methan...
Page 23 - Reagent
23 Leica ASP200 S 3.6.1 Cycle for changing reagents Reagent Week 1 Week 2 Week 3 Week 4 Formalin At least 3 times a week At least 3 times a week At least 3 times a week At least 3 times a week Formalin At least 3 times a week At least 3 times a week At least 3 times a week At least 3 times a week 70...
Page 24 - Initial Operation; must; Installation site requirements
24 Instructions for Use V 1.4, RevE – 03/2013 4. Initial Operation 4.1.1 Moving the instrument After unpacking the instrument (see Unpack- ing instructions on the outside of the transport crate), handle it only by the handles marked with ‘ ‘ to move it to its final location. When operating the instr...
Page 25 - Instrument Setup; Electrical connection
25 Leica ASP200 S 4. Instrument Setup 4.2 Electrical connection • Check the voltage label ( Fig. 3 ) on the rear of the instrument to ensure that the instrument delivered is set to the correct voltage range. Fig. 3 Severe damage may occur if the in- strument is connected to a power sup- ply voltage ...
Page 27 - Active carbon filter; Installing accessories; Remote fill / drain hose
27 Leica ASP200 S 4. Instrument Setup Fig. 5 • Move the instrument to its final setup location. Active carbon filter • Unpack the active carbon filter ( 13 ) and insert it (see Fig. 5 ). When doing so, make sure that the filter is inserted with the correct side up.Note the direction of the label ( 2...
Page 28 - Inserting the volume replacement body; The instrument must be operated only; Magnetic stirrer
28 Instructions for Use V 1.4, RevE – 03/2013 Fig. 8 Inserting the volume replacement body 26 25 27 25 19 Important! The instrument must be operated only when the volume replacement body is in the retort (even during cleaning pro- grams). Otherwise, malfunctions may cause damage to the instrument. •...
Page 29 - Printer; Making the data connections
29 Leica ASP200 S 4. Instrument Setup Fig. 9 Printer (optional) • Swing the door of the instrument console ( 6 ) upwards. • Connect the printer to the printer port ( 8 ) using a Centronics standard cable. ( Fig. 9 ) • Your Leica dealer can recommend a suitable printer. • If compliance with the elect...
Page 30 - Changing the clip
30 Instructions for Use V 1.4, RevE – 03/2013 4. Initial Operation Changing the clip • Once the clip is installed, the handle ( 4 ) of the speci- men basket cannot be removed, as otherwise the clip will be bent. • To remove or replace a clip, all parts that are inside the basket (e.g. spiral insert ...
Page 31 - Remote alarm
31 Leica ASP200 S 4. Instrument Setup Remote alarm This alarm is also external to the Leica ASP200 S. If installed, it might typically be connected to a remote dialer that sends an automated phone message to the person responsible for after- hours problems. The remote alarm is only generated when th...
Page 32 - Switching the instrument on; FAVORITES; The instrument MUST be connected to a grounded power socket.; ON; keep the heater running for the wax baths.
32 Instructions for Use V 1.4, RevE – 03/2013 4.7 Switching the instrument on 4. Initial Operation • After being switched on, the instrument will take a few minutes to initialize. See opposite for cor- responding touchscreen display ( Fig. 14 ). • If the retort is not locked, a signal tone sounds fo...
Page 33 - Queries such as this are always made
33 Leica ASP200 S SYSTEM DIAGNOSTICS menu If TOUCH HERE ... is touched during initialization ( Fig. 14 ) and the pass- word for supervisor mode is en- tered, the SYSTEM DIAGNOSTICS menu ( Fig. 16 ) opens. This menu provides access to basic instru- ment settings. Notice!Only experienced operators may...
Page 34 - Touchscreen functions; Disabled; Button symbols
34 Instructions for Use V 1.4, RevE – 03/2013 4.8 Touchscreen functions The Leica ASP200 S is programmed and operated via a color LCD touchscreen.The instrument's control software contains an online help feature including detailed information about all screen func- tions and error messages. The soft...
Page 35 - Checklist for initial operation
35 Leica ASP200 S 4. Instrument Setup 4.9 Checklist for initial operation Parameter selection Set warning values for the age of the carbon filter and the operating hours of the pressure air pump.Enter the instrument name and select the lan- guage. Optionally, set up the printer. Here, configure all ...
Page 36 - Switching off the instrument; MENU; The instrument is allowed to be switched off completely in this
36 Instructions for Use V 1.4, RevE – 03/2013 4.10 Switching off the instrument If the instrument must be completely switched off or disconnected from the power supply, please proceed as follows: • Press MENU to switch to the MENU FUNCTIONS window. • Press the EXIT APPLICATION button there. A messag...
Page 37 - Setting up the instrument parameters
37 Leica ASP200 S 5. Operation 5.1 Setting up the instrument parameters The SYSTEM SETUP screen is divided up into five selection fields: • Program options • Display/Buzzer • Date/time • Security • Instrument Fig. 24 5.1.1 System setup In the MORE MENU FUNCTIONS press SYSTEM SETUP Fig. 22 From the s...
Page 38 - Date and time setup screen
38 Instructions for Use V 1.4, RevE – 03/2013 PROGRAM OPTIONS Select the options with which programs are executed here. Press a button to change the associated value.• STATION SEQUENCE : By Age or Sequential By Age - RMS is activated, reagents will automatically be used in order of increasing cleanl...
Page 39 - Paraffin temperature and; RUN OPTIONS
39 Leica ASP200 S INSTRUMENT Press PARAFFIN BATH SET TEMP. to open the input screen ( Fig. 30 ) for the wax bath temperature. Set the temperature according to the value required by the paraffin used. Select the highest permissible temperature for the paraffin to ensure that a minimum loss of tempera...
Page 40 - Supervisor mode is disabled in factory condition.
40 Instructions for Use V 1.4, RevE – 03/2013 SECURITY Access rights to the instrument are managed here as user profiles . 5. Operation SUPERVISOR MODE : Enabled or Disabled Enabled - There are two different user profiles. A password is required for supervisor level access to the in- strument. Enabl...
Page 46 - Changing reagent names or reagent groups
46 Instructions for Use V 1.4, RevE – 03/2013 Entering / modifying reagent thresholds If warning thresholds are required for a certain reagent, enter them as fol- lows:• Highlight the reagent to be modified, either by pressing the reagent name or using the UP/DOWN buttons. • Press the header of the ...
Page 50 - Editing program steps; • In the entry windows that pop up, enter / select the program step
50 Instructions for Use V 1.4, RevE – 03/2013 5. Operation Editing program steps • To edit a step, highlight the corresponding line and press the respective headline. • In the entry windows that pop up, enter / select the program step settings. ↓ ↓ ↓ ↓ ↓ Fig. 47
Page 51 - • If required, modify properties of the step.; Remember that a program step cannot be copied if the program al-; • Use the
51 Leica ASP200 S Editing program steps (continued) Setting a delay step A delay step is a step that is extended to ensure that a program will finish at a specified time.• Highlight the program step you wish to define as delay step.• Touch the DELAY button. The delay symbol is moved to the selected ...
Page 53 - The first symbol in the list is empty – you can select this option if; button symbol. The highlighted program will be deleted
53 Leica ASP200 S Assigning a day for the end time If an end time has been assigned to a Favorite program, a specific weekday can also be set on which the program is to end.• Press the DAY button. • Select the desired day from the list and confirm with OK . Assigning a symbol to a "Favorite"...
Page 54 - Only those reagents that are compat-; OK
54 Instructions for Use V 1.4, RevE – 03/2013 5.1.8 Configuring the stations Adding/changing a reagent Select the desired station. Either by pressing the reagent name or using the UP/DOWN buttons.• Touch the REAGENT NAME button. • The SELECT REAGENT field appears. This window ( Fig. 52 ) shows a lis...
Page 55 - button in the table header the; SELECT COLOR; window; • To exit the display without changing any colors, select; CANCEL; program display for each reagent group are displayed.
55 Leica ASP200 S 5.1.9 Reagent groups Fig. 53 Changing the color of a reagent group • Highlight the line containing the reagent to be modified.• Press the COLOR button in the table header; the SELECT COLOR window appears. • Select the color you want and confirm with OK . • To exit the display witho...
Page 58 - see previous chapter
58 Instructions for Use V 1.4, RevE – 03/2013 5. Operation 5.2.2 Replacing the paraffin Draining the paraffin • Connect the paraffin drain hose ( 36 ) to the spout ( 17 ) of the paraffin drain at the front of the instrument ( Fig. 57 ) and into a catch tank. Fig. 57 • Select the paraffin reservoir t...
Page 59 - Filling with paraffin pellets; BACK; The level in a wax bath must NEVER; Software control
59 Leica ASP200 S Filling with paraffin pellets 5. Operation Fig. 60 • Fill the paraffin reservoir to the brim ( Fig. 60 , 1 ) with paraffin pellets. This corresponds to ap- prox. 3.5 kg/bath (Leica Histowax pellets). • Press BACK and REAGENT STATUS to switch to the status table ( Fig. 59 ). Select ...
Page 60 - Filling with liquid paraffin; substantial faults and malfunctions may result.
60 Instructions for Use V 1.4, RevE – 03/2013 Filling with liquid paraffin Software control If the instrument has been filled with liquid paraffin, the instrument's internal calculation of the melting time can be disabled. To do so, in supervisor mode, open the SERVICE FUNCTIONS ( Fig. 61 ) window. ...
Page 61 - Running programs; ALL
61 Leica ASP200 S 5.3 Running programs There are two different windows from which a program can be started: In the Start screen, press the ALL PROGRAMS button. This window displays all of the infiltration programs defined in the instrument. Up to five favorites can be configured in the ASP200 S. A &...
Page 62 - Programs can be started up to 6 days in
62 Instructions for Use V 1.4, RevE – 03/2013 5.3.1 Editing a program that has been called up. Fig. 65 To modify a program before the start, press the EDIT button. A message appears that the changes will affect running programs only. After confirming with YES , the program edit window appears ( Fig....
Page 63 - The options set apply for ALL programs!
63 Leica ASP200 S The RUN OPTIONS function displays the options with which the current program is started. • LEVEL TEST enabled: The lower level sensor in the retort is acti- vated. The program is stopped with an error message if the level is not reached. • WAVE MOTION activated: During each infiltr...
Page 65 - Opening the retort during a program interruption; fore cleaning in order to prevent unnecessary waiting times.; Electronic emergency unlock
65 Leica ASP200 S 5. Operation 5.3.3 Opening the retort during a program interruption Fig. 72 Fig. 73 Fig. 71 • After the signal tone stops, turn the lever all the way to the right ( Fig. 73 ) and open the retort lid. • To unlock the retort, turn the lever for locking/unlocking ( Fig. 71 ) approxi...
Page 66 - Mechanical emergency unlock
66 Instructions for Use V 1.4, RevE – 03/2013 5. Operation 5.3.4 Retort emergency release (continued) Fig. 74 • Turn the retort lock further clockwise using the sin- gle-head wrench ( Fig. 75 , 3 ) and overcome the stop (breaking the retaining pin at the predetermined breaking point). 1 Ensure tha...
Page 70 - troubleshooting system.; and; RUN LOG; lengthened due to the power failure.; Power failure during a retort or paraffin cleaning program
70 Instructions for Use V 1.4, RevE – 03/2013 6. Troubleshooting 6.1 General → For all error states, the instrument takes corrective measures to protect the specimens, except in such cases where continued functioning of the instrument would be possible only under hazard- ous operating conditions. Th...
Page 71 - Troubleshooting; Use the SMART SCREEN functions to individually test operations; for any information relating to the cause.; Power failures; If there is evidence of power to the instrument:
71 Leica ASP200 S 6. Troubleshooting 6.3 Troubleshooting → Use the SMART SCREEN functions to individually test operations such as fill, drain, pressurizing and evacuation operations. If you cannot identify the problem by carrying out the above listed steps, call Leica Technical Service. If a problem...
Page 72 - Typical fill or drain problems; There is insufficient reagent; If you cannot eliminate the problem by carrying out the above list-
72 Instructions for Use V 1.4, RevE – 03/2013 6. Troubleshooting 6.4 Typical fill or drain problems Fill and drain problems may be due to several causes: 1. There is insufficient reagent • Verify that the fill level of the reagent containers and the paraffin reservoirs is sufficient. 2. Insufficient...
Page 73 - Cleaning and Maintenance; EDIT; refer
73 Leica ASP200 S 7. Cleaning and Maintenance 7.1 Clean programs The cleaning programs are at the end of the list of all pro- grams ( Fig. 45 ). To display a clean program, highlight the respective line and press EDIT . Fig. 81 → → 7.1.1 Retort cleaning programs A cleaning program comprises a minimu...
Page 74 - Cleaning and Maintenance; Editing steps; Reagent name; - temperatures from 50 to 65 °C can be set.; matically based on the number of cycles set.; it once it is no longer used.; group. It may only be used for cleaning programs.
74 Instructions for Use V 1.4, RevE – 03/2013 7. Cleaning and Maintenance Editing steps • To edit a program step, highlight the corresponding line and press the respective table header.• To disable a step, set the number of cycles to " 0 ". Only cleaning water steps can be disabled. The foll...
Page 76 - Cleaning and maintenance; SMART CLEAN; ing them into the instrument to make sure there will be no reagent
76 Instructions for Use V 1.4, RevE – 03/2013 7. Cleaning and maintenance 7.1.3 Smart clean The smart cleaning prepares the ASP200 S for transport. During a smart clean, a series of air cleaning steps will be applied which will remove any excess liquid (condensate) from all internal tubing.Before st...
Page 77 - General cleaning steps; Regularly inspect the collecting tray (; Checking the drip tray; Work carefully! Be careful as the walls of the paraffin reservoirs; Instrument exterior; ened with mild detergent and dry.
77 Leica ASP200 S 7.2 General cleaning steps Paraffin reservoirs 7. Cleaning and Maintenance Regularly inspect the collecting tray ( 16 ) for signs of leaking chemicals ( Fig. 84 ). Checking the drip tray Fig. 84 16 Work carefully! Be careful as the walls of the paraffin reservoirs are very hot and ...
Page 78 - Replacing the active carbon filter
78 Instructions for Use V 1.4, RevE – 03/2013 7. Cleaning and Maintenance Replacing the active carbon filter • The life of the active carbon filter will depend on the reagent types used and the frequency of vacuum cycles. • The filter ( 13 ) should be replaced at least every 3 months. ( Fig. 86 ) Af...
Page 79 - Only use the plastic scraper provided when cleaning the retort
79 Leica ASP200 S Cleaning the retort lid • Remove wax from the inside of the retort lid with the plastic scraper provided. Thoroughly remove all wax deposits from around the lid seal. • For convenience, the lid may be removed during cleaning. Lift the lid to a vertical position, release the hinge l...
Page 80 - Cleaning the surface of the infiltration module; chloroform. O-rings that have not been lubricated swell when ex-; Checking the retort lid seal; must replaced without delay.
80 Instructions for Use V 1.4, RevE – 03/2013 7. Cleaning and Maintenance Cleaning the surface of the infiltration module Important in order to ensure that the seals always seal properly.• Remove both covers for cleaning. • First, clean the stainless steel surface using the plastic spatulas, then wi...
Page 81 - Cleaning the reagent containers; their home position at the rear of the reagent module.; are listed in the table on the following pages.
81 Leica ASP200 S Cleaning the reagent containers • Empty and clean the reagent containers. Using a bottle brush and a laboratory detergent in warm water. 7. Cleaning and Maintenance Never clean reagent containers in an automatic dishwasher. The reagent containers are NOT dishwasher-proof! The reage...
Page 82 - Use the cleaning tool together with the microfiber cloth (; wise the sensors will be scratched!; Cleaning the level sensors
82 Instructions for Use V 1.4, RevE – 03/2013 7. Cleaning and Maintenance Fig. 87 7.2.2 Periodic cleaning and maintenance (continued) 44 Fig. 88 Fig. 89 Use the cleaning tool together with the microfiber cloth ( Fig. 87 ) as shown in Fig. 89 . 45 46 47 The level sensors must be cleaned of all residu...
Page 83 - Checklist for preventive maintenance
83 Leica ASP200 S 7. Cleaning and Maintenance 7.3 Checklist for preventive maintenance Step Daily Weekly Monthly every 3 months Lubricate the O-rings of the reagent con- tainer and check them for damage. * * During re- mote filling and draining After exiting the retort cleaning program, wipe the...
Page 85 - Warranty and Service; Warranty
85 Leica ASP200 S 8. Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control proce- dure based on the Leica in-house testing standards, and that the product is faultless and complies with all...
Page 86 - Appendix 1; Notes on Specimen Preparation; embedding cassette being used.; instrument components and thus cause damage.
86 Instructions for Use V 1.4, RevE – 03/2013 Appendix 1 Notes on Specimen Preparation • Cut the tissue samples that are to be embedded to a size that fits the type of tissue and embedding cassette being used. • Use a sharp and clean knife in order to prevent the transmission of contaminants and to ...
Page 88 - Leica Biosystems Nussloch GmbH
www.LeicaBiosystems.com Leica Biosystems Nussloch GmbH Heidelberger Straße 17-19D- 69226 NusslochPhone: +49 6224 - 143 0 Fax: +49 6224 - 143 268 Web: www.LeicaBiosystems.com