Leica RM2125 - Manuals

Leica RM2125 – Manual in PDF format online.

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Manual

Manual Leica RM2125

Summary

Page 2 - Leica RM2125 Troubleshooting Guide; Table of Contents

Leica RM2125 Troubleshooting Guide Table of Contents Problem Page 1. Thick and thin sections 1-2 2. Compressed sections (sections are extremely compressed, wrinkled or jammed together) 3-4 3. Chatter 5-6 4. Scratches or splits in sections 7 5. No specimen advance (no section produced) 8 6. Drifting ...

Page 3 - Problem; The clearance angle is too small.; Schedule a service call

Leica RM2125 Troubleshooting Guide Problem 1. Thick and thin sections a) Partial sections b) Shorter sections in ribbon c) Section thickness varies from one section to another d) Skipping sections e) Varied thickness in one individual section Cause(s) / Corrective Action I) The clearance angle is to...

Page 4 - schedule a service call

Leica RM2125 Troubleshooting Guide Problem 1. Thick and thin sections (continued) a) Partial sections b) Shorter sections in ribbon c) Section thickness varies from one section to another d) Skipping sections e) Varied thickness in one individual section Cause(s) / Corrective Action VI) Backlash in ...

Page 5 - Use a different part of the cutting edge or use a new knife/blade.; II) Blade/knife is gummed up with paraffin; Pre-cool the specimen on a cold plate.; VI) Paraffin buildup on the back of the pressure plate.

Leica RM2125 Troubleshooting Guide Problem 2. Compressed sections (sections are extremely compressed, wrinkled or jammed together) a) Compressed b) Wrinkled c) Jammed Cause(s) / Corrective Action I) Dull knife/blade. a) Use a different part of the cutting edge or use a new knife/blade. II) Blade/kni...

Page 6 - VII) Knife blade is not parallel with pressure plate.; Schedule a service call.

Leica RM2125 Troubleshooting Guide Problem 2. Compressed sections (sections are extremely compressed, wrinkled or jammed together) (continued) a) Compressed b) Wrinkled c) Jammed Cause(s) / Corrective Action VII) Knife blade is not parallel with pressure plate. (cont.) l) Adjust Pressure Plate with ...

Page 7 - Sectioning speed too high.; Rotate the handwheel at a slower speed.; VI) High profile knife may be too thick.; Try using a different brand or grade of blades.; VII) Wax buildup on the back of the front pressure plate.; Clean back of pressure plate.

Leica RM2125 Troubleshooting Guide Problem 3. Chatter a) Venetian Blind affect b) Wash board c) Undulations Cause(s) / Corrective Action I) Sectioning speed too high. a) Rotate the handwheel at a slower speed. II) Clearance angle too big. c) Clearance angle adjustment: Systematically decrease the cl...

Page 8 - X) Vibration in specimen advance system.

Leica RM2125 Troubleshooting Guide Problem 3. Chatter (continued) a) Venetian Blind affect b) Wash board c) Undulations Cause(s) / Corrective Action – Decalcification – Paraffin is too old – Paraffin impurities IX) Tabletop stability. k) Move the microtome or table to a more stable location, or repl...

Page 9 - Try to move the blade or replace it.

Leica RM2125 Troubleshooting Guide Problem 4. Scratches or splits in sections Cause(s) / Corrective Action I) Knife: – Defect in knife edge – Hard specimen in block – Paraffin on front or back of knife edge – Possible staple left in paraffin a) Move to a new area of the knife and observe if the scra...

Page 10 - I) Specimen reached the front feed limit.; Remove any obstruction.

Leica RM2125 Troubleshooting Guide Problem 5. No specimen advance (no section produced) Cause(s) / Corrective Action I) Specimen reached the front feed limit. a) Turn the coarse feed wheel in the appropriate direction to move the specimen towards the rear limit. II) User may accidently be holding th...

Page 11 - a) Adjust the handwheel position and/or collar sleeve position.

Leica RM2125 Troubleshooting Guide Problem 6. Drifting of handwheel Cause(s) / Corrective Action I) Hand wheel is not balanced properly. Handwheel should remain at 12 o’clock when positioned at that location. No drifting should occur. a) Adjust the handwheel position and/or collar sleeve position. S...

Page 12 - Adjust the temperature or humidity level in the room.

Leica RM2125 Troubleshooting Guide Problem 7. Specimen is picked up in the return stroke of the specimen arm Cause(s) / Corrective Action I) Static electricity charge may be built up on the knife holder or specimen head. a) Adjust the temperature or humidity level in the room. b) Use an ionizer to n...

Page 13 - Clean the plate with a swab moistened with Xylene.

Leica RM2125 Troubleshooting Guide Problem 8. Sections sticking to pressure plate Cause(s) / Corrective Action I) Too much paraffin. a) Clean the knife with a swab moistened with Xylene. Stroke the swab in an upward motion away from the blade edge, not down on the blade edge. II) Dirty pressure plat...

Page 14 - I) Retraction mechanism on specimen advance system is defective.

Leica RM2125 Troubleshooting Guide Problem 9. Unable to retract specimen (Note: This is only on the Leica RM2125 RT model) Cause(s) / Corrective Action I) Retraction mechanism on specimen advance system is defective. a) Schedule a service call. 12 back to Table of Contents

Page 15 - I) Linear bearings in cage are at their lower limits.; Linear bearings need to be cleaned and possibly re-greased.; II) Alignment of bearing cage is incorrect.

Leica RM2125 Troubleshooting Guide Problem 10. Microtome makes "clunking noise" when sectioning Cause(s) / Corrective Action I) Linear bearings in cage are at their lower limits. a) Linear bearings need to be cleaned and possibly re-greased. II) Alignment of bearing cage is incorrect. b) Sch...