Leica PELORIS_PELORIS II- Manuals

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 9 Safety Notices The Peloris™ tissue processor is designed to provide safe, trouble-free operation when used in accordance with this document. Follow all safety precautions to avoid personal injury, damage to patient samples,...

Safety Notices Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 10 General Warnings and Cautions Persons operating the Peloris must be fully aware of the following warnings, in order to mitigate possible tissue damage or loss. Reagent Configuration Replacing Reagents Protoc...

Safety Notices Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 11 Instrument Setup Electrical Hazards WARNING Do not use cleaning protocols for reprocessing as the dry step will damage the tissue. WARNING Do not load unprocessed tissue samples into a retort prior to runnin...

Safety Notices Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 12 Reagents WARNING Locate the instrument so that either the mains wall outlet or the instrument’s appliance inlet socket is accessible. You must be able to disconnect the mains power cable without moving the i...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 13 Regulatory Approvals FCC Compliance This device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: 1. This device may not cause harmful interference 2. This device must accept any...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 14 Contents Legal Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2Contacting Leica Microsystems . . . . . . . . . . . . . . . . . . . ...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 17 1 Introduction The Leica Peloris™ rapid tissue processor is a dual retort tissue processor that offers fast, high quality tissue processing for histology laboratories. With reagent capacity sufficient to run the two retort...

Introduction Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 18 1.1 Summary of Chapters  Chapter 1, Introduction : general introduction, chapter summary, general instructions for using the software, and help resources.  Chapter 2, Hardware : descriptions of the main comp...

Introduction Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 19 1.2.1 Basic Operation Buttons Tap buttons to open menus, screens and dialogs, and to start and stop actions in the instrument. Enabled buttons are blue and appear raised. Selected buttons are orange and appear...

Introduction Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 20 Keypads Keypads appear as needed to allow you to enter text and numbers. There are two types of keypad: alphanumeric (text and numbers) and numeric (numbers only). The keypads are analogous to a computer keybo...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 23 2 Hardware The Peloris™ tissue processor is a free-standing processor with two retorts, sixteen reagent bottles and four wax chambers. A touch-screen mounted on the top of the instrument connects to an on-board computer fo...

Hardware Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 24 This chapter describes the main features of Peloris instrument. See sections:  2.1 Switching On and Shutting Down  2.2 Retorts  2.3 Wax Bath  2.4 Reagent Cabinet  2.5 Touch-screen  2.6 External Vapor Removal...

Hardware Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 26 Some instruments have retort latch locks to disallow opening retorts when a protocol is running or the retort temperature is high. See your service representative if you have latch locks on your instrument. Ventin...

Hardware Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 27 well above the markings, but below the maximum (MAX) level. Reagent levels below the minimum will cause protocols to either fail or use a sub-optimal reagent sequence. Liquid Level Sensors Figure 5. Liquid level s...

Hardware Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 29 Opening and Closing Cassette Baskets The lid is held by two catches and is completely removable to aid cassette access.  To remove the lid, release one catch then swing the lid up and off the basket (see Figure 9...

Hardware Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 31 Spaced Baskets The spaced baskets include a fixed divider that ensures that the cassettes are correctly spaced for optimal processing. Each spaced basket can fit up to 72 standard cassettes inserted into the spaci...

Hardware Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 35 2.5 Touch-screen A touch-screen mounted on the right of the Peloris connects to a computer in the instrument that controls all instrument operations. Use the Peloris software on the touch-screen to configure the i...

Hardware Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 36  For instructions on changing to an external vapor system, see Connecting to an External System below.  For instructions on returning to the internal carbon filter system, see Returning to the Internal Filter Sy...

Hardware Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 37 Returning to the Internal Filter System Use the following procedure to reconfigure an externally connected instrument to internal carbon filter use. 1. Ensure a new carbon filter is correctly installed (see 2.4.3 ...

Hardware Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 38 2.7 Alarm Connections Figure 21. Remote alarm (1) and local alarm (2) connectors Each Peloris tissue processor has two external alarm connections — a local alarm connection and a remote alarm connection (see Figur...

Hardware Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 39 Pin Schematic During Normal Operation When the instrument is operating normally (no alarm) the alarm pins connect as shown in Figure 23. Pin Schematic During Alarm Conditions When the instrument has an active alar...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 40 3 Running Protocols This chapter describes how to run protocols on the Peloris™ system. The first section gives all the steps to run a protocol once reagents and protocols have been configured. The second describes cleanin...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 41 2. Check that the instrument is ready: (i) All 16 bottles are properly installed on the instrument.(ii) There is enough reagent in the bottles and wax chambers. Fill to at least the MIN 2 level if you fil...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 42 11. View the progress of the protocol in the Status screen. You can pause processing to add additional cassettes, or abandon protocols completely (see 3.5 Pausing and Abandoning Protocols). Finish Protoco...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 43 3.2 Cleaning Protocols Cleaning protocols clean the retorts and reagent lines. Always run a cleaning protocol as soon as possible after processing runs; unless there are specific reasons not to, always se...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 44 Cleaning Laboratory Implements You can clean metal laboratory implements such as cassette lids and metal molds during cleaning runs, but it is important to set reagent purity thresholds that take this int...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 46 3.3.1 Status Area The status area provides a visual guide to the status of the bottles, wax chambers and retorts, as shown below. Bottle Icons Figure 26. Bottle icons on the Status screen Bottles have six...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 47 Wax Chamber Icons Figure 27. Wax chamber icons on the Status screen Wax chambers have four possible station states: Retort Icons Figure 28. Retort icon on the Status screen Means Icon Full The chamber has...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 50 3.4 Protocol Run Options You can schedule each protocol run so it finishes at a convenient time. You can also modify the protocol for a run, to start at the second or later steps, and to change step durat...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 51 How To Schedule a Protocol Run When the Scheduling dialog box opens the retort’s default end time is shown.  If the default end time is set to ASAP the protocol is scheduled to end at the earliest possib...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 52 Entering a New End Time or Default Scheduling Setting Change the end time for a specific run in the Run protocol – edit end time dialog box, opened from the Scheduling dialog box with the Edit end time bu...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 53 3.4.2 Editing the Protocol for a Single Run You can edit the protocol for an individual run just before the run is started. You can make two types of change: 1. Skip the first steps of the protocol2. Chan...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 54 3.5 Pausing and Abandoning Protocols To stop a protocol that has started use one of the Pause buttons on the Status screen protocol panels. When you tap either Pause button protocols in both retorts stop ...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 55 Emergency Stop and Abandon After you have paused the instrument you can abandon all protocols with the Emergency stop button. Note that this does not shut down or remove power from the instrument (see 2.1...

Running Protocols Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 56 3.6 Retort Scheduling The Peloris tissue processor allows you to simultaneously run protocols in both retorts. The automatic scheduling function attempts to assign reagent stations and start times so that...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 58 4 Protocol Setup Protocols control all aspects of the tissue processing runs carried out on the Peloris. This chapter gives an overview of Peloris protocols and how to create, edit and view protocols with the software inte...

Protocol Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 60 4.1.2 Reagent Selection Method All protocols use one of three reagent selection methods: group, type or station (see 5.1.1 Reagent Groups, Types and Stations for definitions of these terms). Once a protocol ...

Protocol Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 61 Reagent Selection Methods Compared When creating protocols decide which method best suits your processing needs and reagent management strategy. Note, however, that xylene-free protocols cannot use group sel...

Protocol Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 62 Pre-defined protocols can be copied to appear in the Protocol selection screen where they become available for use. They can be copied as they are, even keeping the name unchanged, or once copied you can mod...

Protocol Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 63 Carryover has three main components:  Reagent on retort walls and baskets  Reagent on cassettes, and on and infiltrated into tissue  Reagent on and infiltrated into small-tissue carriers (e.g. biopsy pads...

Protocol Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 64 run uses the respective cassette or pad type. Sample Calculation The average run of a protocol uses the following proportions of tissue carriers:  Surgipath ActivFlo Routine I: 40%  Surgipath Biopsy Wraps ...

Protocol Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 65 4.1.6 Saving Protocol Files Protocols are saved in “cfg” extension files that you can back up or to send to your service representative for troubleshooting. See Transferring Protocols in 6.2.7 File Transfer ...

Protocol Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 66 Protocol Icons On the Protocol selection screen each protocol is represented by a selectable icon. Each icon contains the following protocol details: Figure 38. Protocol icon Icons for the five protocol type...

Protocol Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 68 Selecting Reagents When you select the Reagent cell in the protocol step table to add or change a reagent the dialog box you see depends on the reagent selection method. For all the methods you can show only...

Protocol Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 69 Station Reagent Selection If the protocol uses station reagent selection you can see the stations configured for the instrument. Bottles that are available for selection are drawn in 3D with lids and handles...

Protocol Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 70 4.2.3 Creating New Protocols Create new protocols from scratch or copy an existing or pre-defined protocol to modify. Make sure you select the right protocol type and reagent selection method when you start ...

Protocol Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 71 Copying Protocols You can copy any protocol displayed on the Protocol selection screen in order to create a new one based on it. Alternatively copy a pre-defined protocol. 1. Open the Protocol selection scre...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 72 5 Reagent Setup This chapter gives an overview of Peloris reagent management as well as providing instructions to replace reagents and configure them in the software.  5.1 Reagents Overview  5.2 Managing Reagent Types  ...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 73 5.1.1 Reagent Groups, Types and Stations The Peloris tissue processor manages reagents by groups, types and stations. Groups Groups specify reagent function. For example, the fixatives reagent group includes ...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 74 Reagent type names do not affect reagent concentration. For example, when assigning a reagent type called “Ethanol 70%” to a station, the initial concentration value would be the type’s default value (probabl...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 75  Absolute ethanol (100% dehydrant) contaminated by carryover from absolute IMS (100% dehydrant) has a concentration of 100%, as both the original reagent and the contaminant are dehydrants.  A new xylene (1...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 76 5.1.3 Thresholds Each reagent type has a range of thresholds that supervisors can configure on the Reagent types screen. The thresholds for the pre-defined reagent types should be adequate for most laboratori...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 77 Stations with reagent that has exceeded its change threshold are shown with a hatched icon on the Status screen (bottle icon shown at right). In addition, the system displays a warning message. The Peloris sy...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 78 5.1.4 Recommended Reagents While each laboratory is responsible for its choice of reagents and wax, using reagents other than those recommended for Peloris can result in poor processing or compromise instrume...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 79 5.1.5 Tissue Marking Some laboratories have traditionally added eosin (or other dyes) to formalin or alcohol during tissue processing, to aid in the visualization of tissue during embedding. This practise is ...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 80 5.1.6 Reagent Compatibility As tissue processing requires the use of incompatible reagents, the Peloris tissue processor software uses a set of rules to ensure that only compatible reagents are allowed to mix...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 84 5.3 Managing Reagent Stations The Peloris processor has 20 stations – 16 reagent bottles and four wax chambers. You must set the stations in the software to the reagent types loaded on the instrument. Once th...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 86 5.3.2 Setting Reagent Station Properties See sections:  Assigning New Reagents to Stations  Changing Reagent Concentration  Setting Station States Assigning New Reagents to Stations If you change the type ...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 87 Changing Reagent Concentration You can set a station’s “calculated” concentration value. Tap the station’s Conc. cell. Enter the new concentration in the keypad. Setting Station States All users can change st...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 88 5.4 Replacing Reagents Always replace reagents as soon as possible after the system alerts you. There are two methods to replace reagent in bottles:  Remote fill/drain — pump the old reagent out and new reag...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 91 5.4.3 Replacing Reagent – Remote Fill and Drain You can drain and then refill reagent bottles without removing the bottles from the instrument. The process drains each bottle to a retort then drains the retor...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 92 4. When prompted, check that the retort lid is closed and the remote fill/drain hose is properly connected.Tap OK to begin the drain. 5. The instrument will now drain the bottle(s) via the selected retort. Mo...

Reagent Setup Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 93 12. The instrument will now fill the bottle(s) via the selected retort. The fill volume is determined by the reagent fill level set in the Instrument settings screen (see 6.2.1 Instrument Settings).Monitor th...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 98 6 Settings & Ancillary Operations This chapter describes basic system configuration options and ancillary operations such as manually pressurizing a retort or heating a wax line, viewing the event log, handling alarms,...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 99 6.1.1 Manual Operations The Manual operations screen ( Reagents menu, Manual operations ) allows you to manually control the instrument. The screen displays the current instrument status...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 100 Additional limitations may apply depending on the retort state and the reagent in the retort. You cannot raise the retort temperature above the safe temperature threshold of the reagent...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 101 6.1.2 Reagent Management Use the Reagent management screen ( Reagents menu, Management ) to set the default number of cassettes per run and default carryover setting per run, to turn wa...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 102 Retorts The Retorts section of the Reagent management screen shows the state of and last reagent used for each retort. It also shows the empty access temperature – the temperature up to...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 103 6.2 Control Menu The control screens allow you to alter hardware settings, view event logs and system information, manage alarms and set the access level. The File transfer screen allow...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 104  The inspection interval for the external system  The maximum value (1000) to limit the number of unwanted warnings  Overridden (contact your service representative to arrange this s...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 105 Language and Unit Settings You can view the instrument’s language and temperature and pressure unit settings in the Instrument settings screen. Contact your service representative to ch...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 106 Color Schemes There are two software color schemes – one lighter and one darker. Select the better color scheme for the lighting conditions in your laboratory.  To change the color sch...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 107  Toggle the Show by time/Show by frequency button to switch between the two views.  Tap the Filter button to open the Events log filter dialog box where you set filters to select the ...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 110 Attach or Remove a USB Key Use a USB key to copy files to. Only use the network, FTP or RemoteCare™ options if instructed by a service representative. You can use a floppy disk, but thi...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 111 Transferring Protocols The protocol transfer functions provide an easy way to backup your protocols so they may be restored if lost. The functions also allow you to share protocols acro...

Settings & Ancillary Operations Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 113  To transfer event details tap Event details and set an incident date. Check the Event log screen to identify when the event you are interested in occurred. Select whether to transfer ...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 114 7 Cleaning and Maintenance Follow the maintenance and cleaning instructions in this chapter to keep your Peloris™ processor in top condition. This will help ensure quality processing at all times, and avoid unnecessary se...

Cleaning and Maintenance Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 115 7.1 Cleaning and Maintenance Schedule See the list below for regular cleaning and maintenance tasks and how often they should be carried out. The table on the next page is a weekly maintenance and...

Cleaning and Maintenance Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 116 Leica Peloris™ User Maintenance Chart Week: Serial No: Mon Tues Wed Thurs Fri Sat Sun Date: Daily Tasks Clean lids and seals Clean retorts Check reagent levels Checked = OK Topped off/up = T Chang...

Cleaning and Maintenance Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 117 7.2 Daily Tasks Clean Lids and Seals Use the plastic scraper provided to scrape wax off the inside surfaces of the retort and wax bath lids. Carefully remove wax from around the lid seals. Replace...

Cleaning and Maintenance Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 118 Clean Retorts Clean the retort walls daily with a lint-free cloth moistened with 70% alcohol. Figure 64. Retort interior with: air hole (1), level sensors (2), stirrer (3), and filter (4)  Ensure...

Cleaning and Maintenance Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 119 If the retort has a build up of salt precipitate from formalin or other fixatives that you cannot remove by wiping, run a retort acid clean (7.5 Retort Acid Clean). Check Reagent Levels Ensure tha...

Cleaning and Maintenance Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 120 7.3 Weekly Tasks Empty Condensate Bottle Empty the condensate bottle weekly. This is especially important if running xylene-free protocols, which have more condensate. Check Reagent Bottles Check ...

Cleaning and Maintenance Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 121 Wipe the bottom and sides of the chamber with a lint-free cloth to remove any debris or sludge. Do not remove the chimney filters. Be careful not to dislodge the filters. Take care no material goe...

Cleaning and Maintenance Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 122 7.4 60–90 Days Change Carbon Filter Change the carbon filter every 60 to 90 days. Ensure you have the carbon filter threshold set to a suitable number of days to alert you when filter changes are ...

Cleaning and Maintenance Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 123 Check Lid Seals Inspect the retort and wax bath lid seals every 60–90 days. Replace if worn or damaged. 1. Pull the old seals out of the grooves.2. Clean the grooves if necessary with the wax scra...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 124 8 Reference This chapter has useful reference information to help you set up and operate the instrument.  8.1 Reagent Threshold Guidelines  8.2 Protocols  8.3 Station Configurations  8.4 Protocol Step Temperatures  8...

Reference Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 125 8.1 Reagent Threshold Guidelines The tables in this section list the recommended thresholds for commonly used reagents. There are separate tables for xylene and xylene-free processing: Xylene Processing For the ...

Reference Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 126 8.2 Protocols The protocols in this section have been developed and extensively tested by Leica Microsystems for use on the Peloris tissue processor. Some are included as pre-defined protocols with all Peloris s...

Reference Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 127 The pre-defined protocols are:  Factory 1hr Xylene Standard (see Xylene 1 Hour)  Factory 2hr Xylene Standard (see Xylene 2 Hour)  Factory 4hr Xylene Standard (see Xylene 4 Hour)  Factory 8hr Xylene Standard ...

Reference Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 128 Xylene 2 Hour  Pre-defined protocol “Factory 2hr Xylene Standard“  Carryover setting in pre-defined protocol: 75 Xylene 4 Hour  Pre-defined protocol “Factory 4hr Xylene Standard“  Carryover setting in pre-de...

Reference Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 129 Xylene 6 Hour  Not a pre-defined protocol Xylene 8 Hour  Pre-defined protocol “Factory 8hr Xylene Standard“  Carryover setting in pre-defined protocol: 25 Step Reagent type Reagent group Time (min) Temp (°C) ...

Reference Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 130 Xylene 12 Hour  Pre-defined protocol “Factory 12hr Xylene Standard“  Carryover setting in pre-defined protocol: 25 Step Reagent type Reagent group Time (min) Temp (°C) P/V Stirrer Drip time (s) 1 Formalin Fixa...

Reference Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 134 8.2.5 Cleaning Protocol Quick Clean  Pre-defined protocol “Quick Clean“.  Bottle configuration: one bottle each of cleaning solvent and cleaning alcohol.  Never run a cleaning protocol while tissue is in the ...

Reference Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 135 8.3 Station Configurations Use the station configurations in this section for the protocols described in 8.2 Protocols. If considering alternative station configurations remember to take into account how you wil...

Reference Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 136 Xylene-free Configuration This is the suggested station configuration when running xylene-free protocols (see 8.2.4 Xylene- free Protocols). See note below for special conditions for initial bottle setup. *All a...

Reference Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 137 8.4 Protocol Step Temperatures The Peloris tissue processor uses five protocol types to accommodate different processing functions. Each protocol type has a set of reagent compatibility sequences that comply wit...

Reference Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 138 Standard Protocols Xylene-free Protocols Cleaning Protocols Current step Previous step  = compatible None Fi xa tive s Deh ydr ant s Clear ers Wa x Cleaning solv ents Cleaning alcohols Fixatives    Dehydrant...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 139 9 Troubleshooting If you have processing problems, review this chapter to try to identify the cause.  9.1 Preliminary Questions  9.2 Flowcharts  9.3 Reprocessing Recommendations 9.1 Preliminary Questions When faced wit...

Troubleshooting Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 149 9.3 Reprocessing Recommendations Not all problem tissue requires reprocessing. Some common problems and possible solutions are listed below:1. Tough, Brittle, Over-Processed Tissue2. Gritty Tissue due to C...

Troubleshooting Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 150  20 mL 5% sodium carbonate  Ethanol-glycerol  90 mL 60% ethanol  10 mL glycerol Then process tissue normally. 4. Hard Shriveled Tissue – Dried Out but Processed Through to Wax Remove wax and rehydrate ...

Troubleshooting Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 151 C. Slow Reverse Process Process using a modified cleaning protocol for a period equal to the time required to forward process the tissue (see Slow Reverse Cleaning Protocol below). Then reprocess from form...

Troubleshooting Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 152 Rapid Reverse Cleaning Protocol Slow Reverse Cleaning Protocol Step Reagent type Time (min) Temp (°C) P/V Stirrer Drip time (s) 1 Cleaning solvent 12 65 Ambient High 10 2 Cleaning ethanol 6 55 Ambient High...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 153 10 Consumables and Accessories The following consumable items and accessories are available from Leica Microsystems. Name Part No. Waxsol™ Cleaning Solution (20 liters) S26.0390 Reagent bottle labels S26.6001 Carbon filte...

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 154 11 Specifications Operating Dimensions (H x W x D): ...........................................................................1500 × 857 × 721 mm (59 × 33.7 × 28.4 inches) Weight (dry): .....................................

Specifications Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 155 Environmental Maximum ambient temperature: ........................................................................................ 35 °C Minimum ambient temperature: ..........................................

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 156 Index A abandoning protocols 54 access level 22 setting 108 accessories 153 acid clean for retort 123 active reagent types 80 alarms 107 connections 38 power off setting 105 archiving log files 113 artifact block 148 cutt...