Page 2 - Accessories for measuring tasks
Measuring – User Manual 2 • Stage micrometer (1) for calibration • Graticules with various measuring pitches (2) in mm and inches • Graticule with mesh (3) • Graticule with co-ordinate grid are measured using the graticules with measuringpitches, e.g. 12mm : 120 or 5mm : 100. cells, nuclei and the s...
Page 4 - Setting diopters and grid plate
Co-ordinate the eyepieces precisely to your eyes asdescribed until you can clearly see the graticule andspecimen at the same time (free from parallax). Set distance from eye,eyepieces, illumination etc. as described in the instrument’s directions for use. Set «0» diopters on botheyepieces. Illuminat...
Page 5 - Look into the eyepieces and slightly move your head.
5 Measuring – User Manual Select the lowestmagnification. Observe specimen through theeyepiece with graticule andfocus. Select the highestmagnification. Use focusing drive to optimise sharpness. Do not look into the eyepieces Turn eyepiece without gridplate anticlockwise untilit reaches the stop. Se...
Page 6 - ... if you only undertake relative measurements; Calibrating graticules
6 Measuring – User Manual The graticule is only magnified by the eyepiece.The total magnification of the specimen does howeverdepend on the lens, magnification changer, eyepiece andadditional tube possibly used (e.g. coaxial lighting sources)and changes whenever these factors are altered. When calib...
Page 7 - Focus both pitches down to a single point.; In the example: 0 and 10; eyepiece graticule: number of intervals; Calibrating
7 Measuring – User Manual Place stage micrometer onthe table insert and setsharpness. Select the magnificationwith which you will laterwant to take measurements. Set eyepiece graticule andlens micrometer to be free ofparallax (see page 4). Align stage micrometer (X) close to and in parallel with the...
Page 8 - The area may comprise several squares.; Measuring length
8 Measuring – User Manual Remove the stage micrometer and focus on a specimen. Count how many intervals on the eyepiece graticulecover the measurement distance required. Multiply the number of intervals by the calibrationvalue.The result is the absolute length of the measurementdistance in mm (inch)...
Page 9 - Examples
9 Measuring – User Manual 1. Measuring pitch 12mm : 120 Stage micrometer with 0.1mm graduation Examples Calibrating 7.8mm on the stage micrometer (X) 120 intervals on the eyepiece graticule (Y) 7.8 120 mm = 0.065mm calibration value Measuring Intervals on the eyepiece graticule: 112 Result: 112 inte...
Page 12 - Table
Illustrations, descriptions and technical data are not binding and may be changed without notice. M2-275-0en • © Leica Microsystems (Switzerland) Ltd • CH-9435 Heerbrugg, 2001 • Printed in Switzerland – V .2004 – RDV Instrument Magnification stage Lens Tube factor Eyepieces Graticule Calibration val...