Leica CM3600 - Manuals

8 Instruction manual V 6.0 - 08/2006 1.1 Symbols in the text and their meaning Notes, i.e. important user informationappear in a grey box and are markedwith an information symbol . Buttons and function keys which needto be pushed on the data entry screen,are printed in bold capitals. Figures in brac...

9 Leica CM 3600 – Cryomacrotome 1.4 Designated use The Leica CM3600 is an SPS-controlled and motorized precisioncryomacrotome for large-surface sections, equipped with a convection-cooled cryochamber for deep-freezing, sectioning, storing and freeze-drying large biological and industrial specimens.T...

10 Instruction manual V 6.0 - 08/2006 1.5 Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual prod-uct delivered has been subjected to a comprehensive quality control proce-dure based on the Leica in-house testing standards, and that the product isfaultless and complies with al...

12 Instruction manual V 6.0 - 08/2006 2.1 Layout of the CM3600 PC Knee lever Window Chamber lid Emergency stopswitch Port and switch panel Chamber Refrigerationand control unit Socket panel Microtome(in chamber) Port and switch panel Drain tap 2. Characteristics of the Leica CM3600

13 Leica CM 3600 – Cryomacrotome Drain tap Condensation water is drained through the drain tap. Refrigeration and control unit The refrigeration and control unit cools the cryochamber and controls themicrotome and the entire electronics. Knee lever The knee lever is used for manual operation of the ...

14 Instruction manual V 6.0 - 08/2006 2.2 Technical Data 2.2.1 Microtome type 450 C Sliding microtomeSection thickness selection: 1 - 200 μm Sectioning range: 0 - 450 mm Max. sectioning speed: 80 mm/s Knife retraction after sectioning stroke: 50 - 250 μm Total vertical stroke of knife: 70 mm (standa...

15 Leica CM 3600 – Cryomacrotome 2.2.3 Dimensions and weight Inner (cryochamber L x H x W): 1650 x 720 x 600 mm Outer (L x H x W): 2800 x 1090 x 920 mm (Overall dimensions withconnecting cables)Outer length (total): 2800 mm Outer length (chamber only): 1940 mm Outer length (control unitwith connecti...

17 Leica CM 3600 – Cryomacrotome 2.2.6 General parameters Protective class: I Pollution degree: 2 Overvoltage installation category: II 2.3 Instrument parts The Leica CM3600 consists of a sliding microtome in a large-volumecryochamber. The instrument is designed for cryosectioning large speci-mens o...

20 Instruction manual V 6.0 - 08/2006 3. Safety 3.1 Safety instructions This instruction manual contains important in-structions and information regarding the opera-tional safety and maintenance of the instrument.The instruction manual is an important part of theproduct, which must be read carefully...

21 Leica CM 3600 – Cryomacrotome 3.3 Safety standards The Leica CM3600 was built and tested in accordance with the safetyregulations for electrical measuring, control, regulating and laboratorydevices listed below: • DIN EN 292, • DIN EN 55011, • DIN EN 61010-1, • IEC 1000-4, • IEC 50082-1, • MedGV....

22 Instruction manual V 6.0 - 08/2006 3.4.2 Knee lever Never leave the instrument unattended whenthe knee lever is folded out ( 1 ) to prevent the microtome from being set in motion acciden-tally. Always swing the knee leverupwards before leaving the instru-ment. 3.4.3 Window The window is heatable ...

23 Leica CM 3600 – Cryomacrotome 3.5 Special safety instructions The safety devices installed in the Leica CM3600 by the manufactureronly constitute the basis for accident prevention.Primarily responsible for accident-free operation is above all the institu-tion which owns the instrument and, in add...

24 Instruction manual V 6.0 - 08/2006 As you work through the instruction manual, familiarizingyourself with the instrument, do not yet install a knife orspecimen. Below you find a summary of all warnings contained in this instructionmanual. Any use of the instrument not described in this instructio...

25 Leica CM 3600 – Cryomacrotome Prior to any work involving the knife, microtome or inside thecryochamber, swing the knee lever upwards. For any work involving the knife, microtome or inside thecryochamber always wear special protective gloves. For reasons of safety and accuracy, the maximum trimmi...

26 Instruction manual V 6.0 - 08/2006 4. Site requirements 4.1 Site requirements at place of installation If possible at all, the room should be air-conditioned.Relative humidity ≤ 60 %. Room temperature should not exceed 22 °C. If ambient conditions are not maintained as specified, instrument perfo...

27 Leica CM 3600 – Cryomacrotome For removing the microtome (approx. 190 kgs) from thecryochamber, a ceiling crane capable of carrying that weightshould be available. If no ceiling crane is installed yet, we recommend the specialLeica crane „KR-CM3600“. 4.2 Electrical connections Country: Europe USA...

28 Instruction manual V 6.0 - 08/2006 5.1 Unpacking and installation Only duly trained personnel may unpack and install the Leica CM3600.Please contact your local Leica sales organization for appropriate advice. 5.2 Standard delivery The standard delivery includes: • 1 Leica CM3600, consisting of:• ...

29 Leica CM 3600 – Cryomacrotome 5.3 Port and switch panel Controllamps Connecting block Mains switch Automatic cutout Ground fault circuit interruption Connection for compressed air Connection for foot switch Interface RS 232 Switch for socket panel Externalalarmsignal switch Connection forexternal...

31 Leica CM 3600 – Cryomacrotome GFCI switch Protects the entire instrument. Automatic cutout Protects the refrigerating sets and all electronic components. 5.3.2 Fuses The Leica CM3600 is equipped with the following fuses: • GFCI switch • Automatic cutout • Fuse protecting the mains switch • Fuse p...

32 Instruction manual V 6.0 - 08/2006 Fuse protecting the mains switch If the mains switch fuse has blown, please call TechnicalService. S Fuse protecting socket panel If the socket panel fuse has blown, please proceed as follows: Prior to exchanging the fuse in the socket panel, switch theinstrumen...

33 Leica CM 3600 – Cryomacrotome 5.4 PC The Leica CM3600 is equipped with a commercial PC, monitor, keyboard,mouse and an internal modem. For further information, please refer to themanual of the PC. The control PC as well as all peripherals connected to it(printer, monitor, etc.) are set to an inpu...

34 Instruction manual V 6.0 - 08/2006 6. Software 6.1 Start and log-in procedure Requirements: PC must be on and the Microsoft Windows XP ® operating system must have booted and be ready for service. Doubleclicking on Icon „CM3600“ starts the application. Registration mask 1. Type the user name in t...

35 Leica CM 3600 – Cryomacrotome 6.2 Description of the window elements The window surface is divided into several areas: • Title bar • Tool bar • Work space • Status bar 6.2.1 Title bar The „Title bar“ displays information on instrument name and softwareversion number. 6.2.2 Tool bar The „Tool bar“...

36 Instruction manual V 6.0 - 08/2006 Icons Clicking on the icon opens the „Main window“, which alsoopens up after completion of the start and log-in procedure. Clicking on the icon opens the „Parameter settings“ window. Clicking on the icon opens the „Temperature curve“ window. Clicking on the icon...

37 Leica CM 3600 – Cryomacrotome Clicking on the icon opens the „Label printing“ window. Whenactivated, the icon will light up in green. Clicking on the icon activates the „Foot switch“. When acti-vated, the icon will light up in green and the current sectioningdata will be saved. The ‘Stop’ icon ap...

38 Instruction manual V 6.0 - 08/2006 6.3 Initialization To be able to work with the instrument, after completion of thestart and log-in procedure the microtome must be initialized. For initialization, proceed as follows: 1. In the „Main window“, click on the INIT button blinking in yellow color (in...

40 Instruction manual V 6.0 - 08/2006 6.4 Main window The main window either opens up automatically after comple-tion of the start and log-in procedure or by clicking on the„Main window“ icon. The work space of the main window is divided as follows: • Chamber temperature • Time • Automatic defrost •...

41 Leica CM 3600 – Cryomacrotome 6.4.1 Chamber temperature Indication of set or actual chamber temperature.See chapter 6.5.1 ‘Parameter settings’ on how to set the chamber tem-perature. 6.4.2 Time Indication of real time and date.See chapter 6.5.1 ‘Parameter settings’ on how to set the time. 6.4.3 A...

42 Instruction manual V 6.0 - 08/2006 6.4.4 Automatic dehydration Indication of start time, start date and duration of the automatic dehydra-tion.See chapter 6.5.1 Parameter settings on how to set the automatic dehy-dration parameters. MANUAL DEHYDRATION button Clicking on this button immediately st...

45 Leica CM 3600 – Cryomacrotome 6.4.6 Extraction system The extraction function can only be activated when an extrac-tion unit is connected to the instrument. For further informa-tion, refer to chapter 9.1. Button EXTRACTION ON/OFF in continuous mode Clicking on this button activates or deactivates...

47 Leica CM 3600 – Cryomacrotome 6.4.9 Mode of operation To be able to work with the instrument, after completion of the start andlog-in procedure the microtome must be initialized. For further informa-tion, refer to chapter 6.3. Buttons prior to initialization Buttons after initialization INIT butt...

49 Leica CM 3600 – Cryomacrotome 6.5 Parameter settings Click on „Parameter settings“ to open the correspondingwindow. The work space of the parameter settings window is divided as follows: • Parameter setting • Password list • Configuration • Reference voltages • Language selection • Initialization...

53 Leica CM 3600 – Cryomacrotome Cutting speedReturn speed Indication of cutting and return speed. Click on one of the indications tochange the currently selected value. Values from 0 - 80 mm/sec can beselected. Left block edgeRight block edge Indication of left and right block edge. Click on the in...

55 Leica CM 3600 – Cryomacrotome CLOSE button Clicking on this button closes the ‘User configuration’ window. Doubleclicking on a line in the configuration indication opensthe dialog box „User configuration“. In the „user configuration“ dialog box the following settings can beselected: • No. Indicat...

57 Leica CM 3600 – Cryomacrotome 6.5.3 Configuration To access ‘Configuration’, the registered user must be anadministrator. The „Configuration“ window contains the following tabs: • Text main study • Text substudy • Measured values • General • Labels • Comments OK button Clicking on this button sav...

59 Leica CM 3600 – Cryomacrotome For the configuration of a main or substudy see chapter 6.10. The following measured values can be selected: • Set chamber temperature • Actual chamber temperature • Alarm temperature • Set section thickness • Actual section thickness • Knife holder position • Dehydr...

61 Leica CM 3600 – Cryomacrotome Die export file is filed as a text file and according to the export pathselected. Millitron Provided an external Millitron measuring device is connected to the PC,the corresponding serial connecting port can be selected via the dropdown menu. Activating the millitron...

63 Leica CM 3600 – Cryomacrotome „Comments“ tab Frequently used comments can be entered into this tab. Button NEW Clicking on this indication opens the dialog box ‘Comment on section’ Here you can enter a comment on a section. Clicking on OK saves the entry and closes the window. Clicking on Cancel ...

65 Leica CM 3600 – Cryomacrotome 6.6 Temperature curves Click on the temperature curve symbol to open the correspond-ing window. The window contains the following window panes: • Work space • Diagram The „Temperature curves“ window is only for failure analysisby Leica Service engineers. 6. Software ...

69 Leica CM 3600 – Cryomacrotome 6.7 Chronological event list Click on „Event list“ to open the corresponding window. The events list is a table displaying the events chronologically, in order ofdate and time. In this list all events concerning the program are saved. The work space is divided up as ...

71 Leica CM 3600 – Cryomacrotome Comment Clicking on an event in the events list activates the „Comment“ field. Youcan enter a comment regarding the selected event. After confirmationwith the ENTER key, the note will be displayed in the event table. Number Number of events displayed in the events li...

73 Leica CM 3600 – Cryomacrotome 6.8 Alarm list Click on the „Alarm list“ symbol to open the correspondingwindow. Chronological display of the alarm list as a table, in order of date andtime. In this list, all events concerning the program are saved. The window contains the following window panes: •...

75 Leica CM 3600 – Cryomacrotome 6.8.2 Event table The table is divided into the following columns: • Occurred Indication of date and time when the alarm started • Cleared Indication of date and time when the alarm stopped • Confirmed Indication of date and time when the registered user confirmed th...

77 Leica CM 3600 – Cryomacrotome 6.9 Chamber illumination Click on the symbol to switch on the illumination of thecryochamber. Another mouse click will switch the chamberillumination back off. Active icons will light up in yellow. 6.10 Section documentation Click on the ‘Section documentation’ symbo...

81 Leica CM 3600 – Cryomacrotome • Comment Comments can be entered by the user • Knife holder position, Knife holder position at the time the section was made 6. Software

83 Leica CM 3600 – Cryomacrotome 6.11 Photo mode Clicking on the photo symbol activates or deactivates the„Photo mode“ and moves the microtome sledge to the me-chanical limit stop. When active, the symbol will light up ingreen. The photo mode enables you to position the specimen or rather themicroto...

84 Instruction manual V 6.0 - 08/2006 6.13 Remote inquiry Clicking on the „Remote inquiry“ symbol opens the dialog box‘Safety check - initialization’. Carry out the required safety query and subsequently confirm each safetyquery by mouse click on the corresponding check box. Having confirmedall safe...

85 Leica CM 3600 – Cryomacrotome To establish a connection proceed as follows: 1. Doubleclicking on Icon „CM3600“ establishes the connection. The „pcAnywhere window waiting“ window will open up. Click on CANCEL to stop the connection from being established. After the connection has been successfully...

87 Leica CM 3600 – Cryomacrotome 6.18 User log-in / log-out Clicking on the symbol logs off the user currently logged in.Click again to open the ‘Start-and-login’ window. For furtherinformation, refer to chapter 6.2. Logging off does not automatically stop or delete the actionsand settings initiated...

88 Instruction manual V 6.0 - 08/2006 7. Operating the Leica CM3600 To ensure proper operation of the Leica CM3600 as well as useof the specimen stages and the specimen blocks themselves,we strongly recommend to attend a Leica application training. 7.1 Check list The checklist below provides an over...

89 Leica CM 3600 – Cryomacrotome 7.2 Switching the instrument on Proceed as follows: 1. Switch on the mains switch. For further information, refer to chapter 5.3. The refrigeration and control unit is activated. 2. Switch on the socket panel switch. For further information, refer to chapter 5.3. 3. ...

90 Instruction manual V 6.0 - 08/2006 7.5 Specimen stages This chapter provides instructions on how to insert the knives. There are three different types of specimen stages: • Special holder, orientable, with ball-and-socket joint, • Specimen stage, orientable, with ball-and-socket joint, • Specimen...

91 Leica CM 3600 – Cryomacrotome When inserting the specimen stages, bear in mind the following: • Prior to sectioning, the specimen stage should have been storedinside the cryochamber for at least 24 hours, to make sure it has thecorrect sectioning temperature (ca. -20° C). • To mount the entire bl...

96 Instruction manual V 6.0 - 08/2006 7.6 Knife This chapter provides instructions on how to insert the knives. Two types of knives can be used in the CM3600: • Standard knives • Disposable blade holder with disposable blade Since there is a considerable risk of injury, please be sure to comply with...

103 Leica CM 3600 – Cryomacrotome 7.7 Setting the trimming parameters Proceed as follows: 1. On the computer screen, open the ‘Main window’. Select the desired section thickness, sledge speed, cutting window and sectioningprogram. For further information, refer to chapter 6.4. For reasons of safety ...

104 Instruction manual V 6.0 - 08/2006 7.8 Setting the sectioning parameters Proceed as follows: 1. On the computer screen, open the „Main window“, select „ MANUAL “ mode of operation. Subsequently, select the desired section thicknessand the cutting window. For further information, refer to chapter...

105 Leica CM 3600 – Cryomacrotome Proceed as follows: 1. Select manual mode of operation and the desired section thickness.2. Activate the section documentation function.3. Use the knee lever to move the sledge to the right-side reversal point (left edge of the specimen block just about clears the k...

106 Instruction manual V 6.0 - 08/2006 16. Attach the section to a dehydration frame which has already been kept in the cryochamber for a while. 17. To speed up the dehydration process once you are done with the sectioning work, the automatic or manual dehydration function of theinstrument can be ac...

108 Instruction manual V 6.0 - 08/2006 8.1 Cleaning For cleaning and disinfection of the stainless steel cryochamber and themicrotome, use commercial alcoholic disinfectants or alcohol.The outer surfaces of the cryochamber and the control unit can becleaned with mild household cleaners or with alcoh...

109 Leica CM 3600 – Cryomacrotome 2. Switch the instrument off and unplug from mains, see chapter 5.3.1. 3. Extract the knife from the knife holder, see chapter 7.6. 4. Lock the window, see chapter 3.4.3. 5 Open the cabinet lid, see chapter 3.4.4. 6. Remove screws ( 1 ) and screws ( 2 ). When removi...

110 Instruction manual V 6.0 - 08/2006 8.2 Maintenance 8.2.1 Oiling Be sure to check the oil level every and before each section-ing procedure. Checking the oil level Unscrew both lids ( 1 ). The oil level must always be above the small capillary tubes ( 2 ). Filling up the oil reservoirs Unscrew bo...

111 Leica CM 3600 – Cryomacrotome 8.2.2 Defrosting Three different defrost procedures can be selected: • Automatic defrost I• Automatic defrost II• Manual defrost Automatic defrost I This defrost cycle takes place automatically once every 24 hours. Thedefrost cycle itself is mandatory, i.e. cannot b...

112 Instruction manual V 6.0 - 08/2006 9.1 Extraction system The Leica CM3600 can be optionally equipped with an extraction device.This device greatly reduces the buildup of cutting waste in the cryocham-ber and microtome and simplifies cleaning of the instrument. To operatethe extraction device, ac...

113 Leica CM 3600 – Cryomacrotome Function The extraction device is mounted sideways to the knife holder ( 6 ). Extraction is achieved by a nozzle system located in the housing ( 5 ), which is powered by compressed air (5 bar).To facilitate optimal removal of cutting waste, the swivel-mounted extrac...

115 Leica CM 3600 – Cryomacrotome 9.2 Ordering information Specimen stages Specimen stage, non-orientable, 40 x 80 mm 0022 50103 Specimen stage, non-orientable, 80 x 180 mm 0022 50105 Specimen stage, orientable,with ball-and-socket joint 120 x 200 mm 0022 50106 Specimen stage, orientable,with ball-a...

117 Leica CM 3600 – Cryomacrotome Section collecting tape Section collecting tape, 38 mm x 66 m, type 810 7022 50203 Section collecting tape, 76 mm x 66 m, type 810 7022 50206 Section collecting tape, 148 mm x 66 m, type 810 7022 50215 Section collecting tape, 110 mm x 66 m, type 4248 0391 24390 Sec...