Leica CM3050 S- Manuals

8 Instruction Manual V1.2 - 11/2006 General This instruction manual includes importantinstructions and information related to theo p e r a t i n g s a f e t y a n d m a i n t e n a n c e o f t h einstrument. The instruction manual is an important part of theproduct. It must be read carefully before ...

9 Leica CM 3050 S – Cryostat 2.1 General information on instrumentdesign and safe handling This instrument has been built and tested in ac-cordance with the following safety regulationson electrical measuring, control, regulating andlaboratory devices: • DIN EN 292 • DIN EN 61010-1 • EN 50082-1 • EN...

10 Instruction Manual V1.2 - 11/2006 Instruments with sectioning motor: The sectioning motor is now blocked. All instruments: The message ‘LOCKED’ in the display of controlpanel 1 indicates that the handwheel has beenlocked: Unlocking: • To unlock, push locking pin ( 2 ) to the left into position ( ...

11 Leica CM 3050 S – Cryostat 2.2.3 Emergency stop function (instruments with sectioningmotor only) The emergency stop is activated via the redemergency stop button in control panel 2 or viathe footswitch Activating the emergency stop function • Press emergency stop button or step onfootswitch force...

12 Instruction Manual V1.2 - 11/2006 2. Safety 2.3 Safety instructions: handling and operation of the instrument 2.3.1 Transport • To avoid severe damage to the instrument byrunning it while the compressor oil is dis-placed from its regular position:• Do not tilt the instrument,only transport in an ...

13 Leica CM 3050 S – Cryostat 2.3.4 Handling microtome knives/blades • Danger!--> Microtome knives and disposable blades have extremely sharp cutting edges andcan cause serious injuries. Therefore: • Handle knives / blades with utmost care. • Never leave any knives / blades in unpro-tected places...

14 Instruction Manual V1.2 - 11/2006 2. Safety 2.3.9 Infectious/radioactive material • Danger!Use caution when working with potentiallyinfectious specimens:-->Risk of infection! • When working with potentially infectious /radioactive specimens: • Wear protective clothes (gloves, protec-tive boots...

15 Leica CM 3050 S – Cryostat 2. Safety 2.3.11 Removing/reinstalling the microtome • Before removing the microtome: • Switch instrument off. • Unplug from mains. • Place handwheel grip in lowest position andlock. --> When removing the microtome, the specimen head must always be lok-ked in the low...

16 Instruction Manual V1.2 - 11/2006 3.2 Unpacking and installation Unpacking instructions are always located in atransparent protective envelope on the outsideof the instrument shipping crate. Make sure to read and follow allsafety instructions provided inchapter 2.3.1 ‘Transport’ and on theunpacki...

17 Leica CM 3050 S – Cryostat 3. Installation 3.3 Standard delivery 1 Cryostat with microtome ............................................................................. 0460 32037 1 Handwheel with marking, antibacterial ................................................... 0471 42558 1 Heat extract...

18 Instruction Manual V1.2 - 11/2006 Further accessories: Further accessories which you ordered will be included in the box containingthe standard delivery items. Knife holders are delivered with anti-roll guide, knife guard, and aseparate instruction manual.In case of non-configured instruments, th...

19 Leica CM 3050 S – Cryostat 3.4 Installing/inserting accessories 3.4.1 Installing the handwheel • Insert pin ( 1 ) of the handwheel shaft into hole ( 2 ). • Place spring washer ( 3 ) onto screw ( 4 ) as shown. • Tighten screw ( 4 ) with an Allen key. • Cover with selfadhesive disc ( 5 ). For purpo...

20 Instruction Manual V1.2 - 11/2006 3.4.3 The footswitch Function The footswitch performs the same task as the RUN/STOP and RUN/ENABLE keys (activating/ deactivating motorized sectioning / trimming).In addition, the footswitch can be used to acti-vate the emergency stop function. Models with footsw...

21 Leica CM 3050 S – Cryostat 4. Instrument Properties 4.1 Overview 1 Cryostat CM3050 S 2 Control panel 1 3 Control panel 2 4 Storage shelf, left 5 Storage shelf, right 6 Rubber mat 7 Mains switch /Automatic cutoutfor instrument,Automatic cutoutfor sectioning motor,Footswitch port 8 Quick-freeze she...

23 Leica CM 3050 S – Cryostat 4. Instrument properties Operating temperature range (ambient temperature): + 18°C to + 40°C. All specifications related to tempe- rature are valid only up to an ambient temperature of + 22°C and relative air humidity of less than 60 %! Refrigeration system 50 Hz 60 Hz ...

24 Instruction Manual V1.2 - 11/2006 4. Instrument properties Microtome Rotary microtomeSection thickness setting 0.5 - 300 μm Overall specimen feed 25 mm Vertical stroke 59 mm Specimen retraction 50 μm Maximum specimen size 40 x 55 mm Specimen orientation 8° (x-, y-, z-axis) Trimming (6 thickness s...

25 Leica CM 3050 S – Cryostat 5. Operation 5.1 Operating the instrument for the first time Prior to setting up the instrument, make sure to read the instruction manualcarefully and in full. When setting up the instrument, please follow the individual chapters in thefollowing order: • First, study ch...

26 Instruction Manual V1.2 - 11/2006 5.2 Switching on/fuses Switching on • To switch on the instrument, place switch ( 1 ) in upper lock-in position. Mains switch / automatic cutout for theinstrument: In addition to its function as ON/OFF switch, switch ( 1 ) is also the automatic cutout for the in-...

27 Leica CM 3050 S – Cryostat 5.3.1 Key functions in control panel 1 5.3 Control panel 1 ON/OFF key for cryochamber illumination. To select the menu items for setting /changing instrument parameters. In the status indication, pressing the ar-row keys displays the individual currentparameters.In the ...

28 Instruction Manual V1.2 - 11/2006 5. Operation Meaning of indication: Operator activity: yes no no Acknowledge indication bypressing once briefly. Initialization phase 1 Initialization phase 2 Software version Selected display language Sectioning motor speedlevel ‘slow’ / (fast) --- --- --- Selec...

29 Leica CM 3050 S – Cryostat 5. Operation Once the instrument has gone through the initialsequence of indications, you can go on to themenu functions to select and/or change instru-ment parameters. If display is locked:Press key until display backgroundlights up. Next, press the menu key. With each...

30 Instruction Manual V1.2 - 11/2006 5. Operation 2. Cryochamber temperature: Press key until menu item ‘Set chambertemperature’ is displayed: --> Continue as described for specimen temperature. 3. Preset counter (instruments with sectioning motor only): Press key until menu item ‘Presetcounter’ ...

31 Leica CM 3050 S – Cryostat The automatic defrost cycle takes place onceevery 24 hours at the selected defrost time. 7. Duration of defrost cycle: Press key until menu item ‘Defrost period’is displayed: • Use defrost keys ( or ) to adjust de- frost time. (Only if necessary - see below). Important ...

32 Instruction Manual V1.2 - 11/2006 5. Operation 5.3.5 Status display Each individual parameter is displayed in a certain area of the display screen. Digits 1 and 2 indicate sectioning stroke position: • Sectioning stroke (outside sectioning window) • : Retraction stroke • [ ] : Sectioning stroke (...

33 Leica CM 3050 S – Cryostat 5. Operation These parameters can be displayed via the ar-row keys.When pressing , the parameters are dis- played in the sequence listed on this page;when pressing , they are displayed in re- verse order. The display can be scrolled contin-uously, i.e. after the last pa...

34 Instruction Manual V1.2 - 11/2006 5. Operation 5.4 Control panel 2 Functional area A:• Sectioning modes• Sectioning window• Sectioning speed• Emergency stop function Functional area B:• Coarse feed• Trimming• Section thickness selection Functional area C:• Start / Stop sectioning Instruments with...

35 Leica CM 3050 S – Cryostat Start / stop motorized sectioning Sectioning mode 1 - Intermittent stroke: • Simultaneously press and hold RUN/STOP andRUN/ENABLE or depress and hold footswitch. • To stop the sectioning stroke, release thekeys or the footswitch:The specimen head stops immediately. Sect...

36 Instruction Manual V1.2 - 11/2006 The value has been stored when the LED in thesectioning window key lights up briefly. If the LED flashes, the value has not yet beenstored, because the user has tried to set a win-dow margin outside the possible window range(the area in which the sectioning windo...

37 Leica CM 3050 S – Cryostat 5.4.3 Selecting sectioning speed • Sectioning speed is selected via the slidingpotentiometer. Speed can be changed whilesectioning is in motion. Caution when working in continuous mode: If the slider is set to ‘0’, the sectioning motionstops, however, the actual section...

38 Instruction Manual V1.2 - 11/2006 5. Operation 5.4.5 Coarse feed keys The four coarse feed keys in functional area Bfunction as follows: Coarse feed ‘fast backward’ - Travelling speed 1,000 μm/s • Press key to activate. Specimen head travels backward(= away from knife): a. until it reaches the re...

39 Leica CM 3050 S – Cryostat 5. Operation Press this key to alternate between thetwo functions. illuminated:Trimming mode is active off:Sectioning mode is active. Selecting trimming thickness • Activate trimming mode • Select trimming thickness (= sectioningthickness at which trimming is carried ou...

40 Instruction Manual V1.2 - 11/2006 5.5 Daily operation of the instrument • Select chamber temperature and, in instru-ments with specimen cooling, specimentemperature according to the type of speci-men to be sectioned (see also chapter 6.3‘Reference chart for temperature settings’). • Do not contin...

41 Leica CM 3050 S – Cryostat 5.5.2 Freezing specimens onto specimen In instruments with specimen cooling, the 90 °prism, which is inserted into the specimen head,is an additional option to freeze specimens atmaximum temperature . Installing the 90 ° prism • Loosen screw (3) at specimen head. • Inse...

42 Instruction Manual V1.2 - 11/2006 5. Operation 5.5.4 Inserting specimen discs into the • Lock handwheel in upper position. If a knife has already been insertedinto the knife holder, cover knifeedge with knife guard! • Loosen clamping screw (3). • Insert specimen disc in opening (2) (rearsurface o...

43 Leica CM 3050 S – Cryostat 5. Operation Inserting the knife • Insert the knife / disposable blade into theknife holder. • Select appropriate clearance angle at knifeholder (settings between 4° and 6° are suit-able for the majority of applications). For details on how to insert and ad-just the kni...

44 Instruction Manual V1.2 - 11/2006 5. Operation 5.6 Finishing work 5.6.1 Daily routine steps Remove all specimens from the cry-ochamber and store safely! S e e also corresponding safety instruc-tions in chapter 2.3.7 ‘Defrosting /handling frozen tissue’! • Lock handwheel. • Remove knife from knife...

45 Leica CM 3050 S – Cryostat H O M E 5.7 Defrosting The Leica CM3050 S has 3 (instruments withoutspecimen cooling: 2) different defrosting options. - Automatic chamber defrost cycle - Manual chamber defrost cycle - Specimen head defrosting (manual) - instru-ments with specimen cooling only. Read th...

46 Instruction Manual V1.2 - 11/2006 S E T T E M P O T - 3 5 ° C S E T T E M P C T - 3 0 ° C - 5. Operation 5.7.2 Manual chamber defrost cycle In addition to the automatic defrost cycle, amanual chamber defrost cycle can be activat-ed when necessary. • Press until menu item ‘Set chamber temperature’...

47 Leica CM 3050 S – Cryostat 6. Troubleshooting, applications tips 6.1 Displayed error messages The autodiagnosis function of the CM3050 Sdisplays error messages in case of certain mal-functions. 1. Power failure This error message is displayed after the instru-ment had been disconnected from mains...

48 Instruction Manual V1.1 02/2000 6. Troubleshooting, applications tips 6.2 Potential problems - causes and remedies Remedies - Eliminate draft sources or change place of installation ofthe cryostat. - Close sliding window. - Consider wearing a mask. - Select lower temperature.- Wait until knife an...

49 Leica CM 3050 S – Cryostat 6. Troubleshooting, applications tips Problem Sections curl on anti-roll plate. Scraping noise during section-ing stroke and specimen returnstroke. Ondulated sections. Chatter marks form duringsectioning. Condensation forms on anti-rollplate and knife during cleaning. C...

50 Instruction Manual V1.1 02/2000 6. Troubleshooting, applications tips Causes - Anti-roll plate protrudes too far beyond the knife edge. Adjust-ment was done in the directionof the knife. - Wrong temperature for the tissue to be sectioned. - Knife profile inappropriate for the specimen to be secti...

52 Instruction Manual V1.1 02/2000 6. Troubleshooting, applications tips 6.3 Reference chart for temperature settings (in minus °C) Tissue 10 - 15 15 - 25 25 - 50 Adrenal Bone marrow ❄ Brain Bladder Breast - fatty Breast - little fat Cartilage Cervical Fatty Heart and vascular Intestinal Kidney Lary...

53 Leica CM 3050 S – Cryostat 7. Disinfection, cleaning and maintenance 7.1 Safety instructions When disinfecting the instrument,make sure to observe the followingsafety instructions: • ‘Disinfection and Cleaning’ (Chapter 2.3.10) • ‘Handling microtome knives / blades’(Chapter 2.3.4) • ‘Defrosting /...

54 Instruction Manual V1.2 - 11/2006 7 Disinfection, cleaning and maintenance 7.3 Disinfection with conventional • Switch the instrument off. • Carefully remove knife / blade as well as spec-imens and accessories from the chamber. • Remove section waste from the chamberand dispose of it. • Remove sl...

55 Leica CM 3050 S – Cryostat 7.5 Removing / reinstalling the microtome 7.5.1 Removing the microtome When removing the microtomemake sure to observe the followingsafety instructions: • ‘Removing / reinstalling the microtome’(Chapter 2.3.11) • ‘Defrosting / handling frozen tissue’(Chapter 2.3.7) • ‘I...

56 Instruction Manual V1.2 - 11/2006 7.5.3 Reinstalling the microtome When reinstalling the microtomemake sure to observe the followingsafety instructions: • ‘Removing / reinstalling the microtome’(Chapter 2.3.11) • Display message ‘Dry Microtome’ (Chapter 2.3.12) • Lock handwheel in lower position....

57 Leica CM 3050 S – Cryostat Removal • The lamp (3) is located behind the glareshield (4). • Carefully hold lamp with both of your hands.Slightly pull the lamp downward at the leftside to remove it from fastening clip (5). • Pull the lamp towards the left to remove itfrom its socket (6). Installing...

58 Instruction Manual V1.2 - 11/2006 8. Warranty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual prod-uct delivered has been subjected to a comprehensive quality controlprocedure based on the Leica in-house testing standards, and that the prod-uct is faultless a...

59 Leica CM 3050 S – Cryostat An electrohydraulic lifting device for individualworking height adjustment is available as anoptional accessory for the CM3050 S cryostatseries. 1.1 Designated use The electrohydraulic height adjustment devicehas been designed exclusively for adjustmentof the instrument...

60 Instruction Manual V1.2 - 11/2006 9. Appendix 1.4 Installation • Open the shipping box and remove all packing material including the Sty-rofoam pieces. • Take the height adjustment device out of the box. • At the place of installation, place the U-frame on the floor with the openside facing yours...

61 Leica CM 3050 S – Cryostat The control keys are located on top of the con-trol unit (see photograph). • To lift the instrument, press . • To lower the instrument, press . As long as a button is held, the instrument willgo up or down until reaching the upper or lowerlimit. 1.6 Maintenance and care...

62 Instruction Manual V1.2 - 11/2006 2.1 Knife holders Knife-holder CN for reusable microtome knives´w/anti-roll guide, adjustable knife guard and knife support bars for slim and wide knives ............................................. 0419 18649Knife support bar for knife holder CN, for slim knive...

. . . . . . . . . . . . . . . . . . . . . . . . . . . . Dag Graupner Managing Director We herewith declare, in exclusive responsibility, that the Leica CM3050 S – Cryostat was developed, designed and manufactured to conform with the• Council Directive 73/23/EEC (Low Voltage), • Council Directive 89/...

64 Instruction Manual V1.2 - 11/2006 11. Decontamination Certificate (master) Dear Customer,any product which is be returned to Leica Microsystems or serviced on site, must be cleaned anddecontaminated in the appropriate manner. This certificate, duly completed, must be placed in the unitprior to pa...

65 Leica CM 3050 S – Cryostat 11. Decontamination Certificate (master) Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging or hand it over to the service engineer. Customer as-sumes all responsibility for the immediate return shipment of articles sent to Leica wit...