Leica ASP300- Manuals

8 Instruction manual V 1.3 – 12/2003 2. Safety 2.2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basisfor accident prevention.Primarily responsible for accident-free operation is above all the institution which owns theinstrument and, in addition, t...

9 Leica ASP300 Warnings - working with the instrument The Leica ASP300 may only be operated by trained laboratory personnel and as per thepresent instruction manual. In case of emergency switch off mains (item no. 5 in Fig. 3) at the rear of the instrument andunplug the power cord.The instrument mus...

10 Instruction manual V 1.3 – 12/2003 2.3 Integrated safety devices The instrument is equipped with the following protection features: • overpressure protection, • protection against excessive vacuum, • overcurrent protection, • overheating protection. Overpressure protection The design of the press...

11 Leica ASP300 3. Instrument components and specifications 3.1 Specific instrument options • R eagent M anagement S ystem (RMS). • The specimen cassette baskets may be used with or without cas-sette dividers and spring separators designed to separate cassettesand improve fluid penetration. • In-pro...

13 Leica ASP300 Overview - Instrument components 1 - Basic instrument - processor module 2 - Basic instrument - reagent module 3 - Wax bath lids 4 - Retort lid 5 - Touch screen 6 - Cover flap of instrument console 7 - Instrument console comprising: 8 - Printer port 9 - Local alarm connection 1 0 - R...

17 Leica ASP300 3. Instrument components and specifications 3.4 Technical Data (continued) Hardware and Software: • Large color liquid crystal display (LCD) and touchscreen. • User-friendly, intelligent software. • 3,5“ floppy drive and printer port. • Alarm system with two remote alarm sockets. • P...

18 Instruction manual V 1.3 – 12/2003 3.5 Compatible reagents Fixatives 1. Formalin, buffered or unbuffered2. Formalin saline Dehydration 1. Ethanol 4. Butanol 2. Isopropanol 5. Industrial Methylated Spirits 3. Methanol Clearing 1. Xylen (recommended)2. Toluol * 3. Chloroform * Paraffin wax 1. Paraf...

19 Leica ASP300 Moving the instrument The instrument must be installed such that the mains switch atthe rear of the instrument (item no. 5 in Fig. 3) can easily be ac-cessed at any time. After the instrument has been unpacked (see unpacking instructionsattached to outside of transport crate) it must...

20 Instruction manual V 1.3 – 12/2003 4. Setting up the instrument • Connect mains cable ( 6 ) to mains power supply socket ( 1 ) (see Fig. 3 ). • Jumper cable ( 3 ) connects the mains power supply output ( 2 ) to the electronics module input ( 4 ). • Switch ON/OFF switch ( 5 ) ON. • Switch ON/STOP ...

21 Leica ASP300 4. Setting up the instrument • Unpack the activated carbon filter and in-sert it into the instrument (see Fig. 4 ). Make sure to insert the filter with the correct sidefacing upwards. See label ( 6 ) at the front of the filter - the arrow must point upwards. • Unpack the magnetic sti...

22 Instruction Manual V 1.3 – 12/2003 4.4 Setting up the data connections Printer (optional) • Lift off the instrument console flap ( 12 ). • Connect the printer to the printer port ( 7 ) via a standard Centronics cable (refer Fig. 6 ). Other connections • For additional data transfer and storage th...

24 Instruction Manual V 1.3 – 12/2003 • After switching it on the instrument will takea few minutes to initialize (see opposite - Fig. 7 ). • After initialization, the FAVORITES screen will be displayed ( Fig. 8 ). Screen saver • A screen saver will operate turning thescreen off when no key has been...

26 Instruction manual V 1.3 – 12/2003 4.7 Touch screen functions 4.7.1 User interface - overview The Leica ASP300 is programmed and operated via a colored LCDtouch screen. An on-line help feature provides detailed information on all instrumentfunctions. HELP can be accessed from any screen by pressi...

28 Instruction manual V 1.3 – 12/2003 4. Setting up the instrument 4.8 Check list for first operation (at supervisor access level) Parameter settings E n t e r i n s t r u m e n t n a m e a n d s e l e c t l a n -guage.Activate printer (optional). Enter carbon filter age warning and pumpwarning thre...

29 Leica ASP300 5.1 Reagent handling 5.1.1 Fill/drain reagents (other than wax) 1. Manually changing the reagents • Remove the appropriate reagent bottle from the Leica ASP300 re-agent cabinet, remove the bottle screw cap. • Drain/refill the reagent by pouring to/from a bulk container. • On the REAG...

30 Instruction Manual V 1.3 – 12/2003 Select station Press key symbol Fig. 12 → 5. Operation Remote drain • On the screen, select reagent bottle no. 3 and the bulk container ( Remote ). The press STATION DRAIN . • Check for leaks. Remote fill • On the screen, select reagent bottle no. 3 and the bulk...

31 Leica ASP300 5. Operation 5.1.2 Changing the wax Draining the wax • Connect the paraffin drain hose ( 2 ) as shown in Fig. 13 and insert the loose end of the hose into a remote collecting container. Fig. 13 • Select the wax bath to be drained and theremote container (‘Remote’).Then press STATION ...

32 Instruction Manual V 1.3 – 12/2003 Fig. 14 → → • Remove the splash protector. • Fill the wax bath ( Fig. 15 ) with wax pellets up to the top. • Press BACK and REAGENT STATUS to ac- cess the reagent status table ( Fig. 14 ). High- light the wax bath you just filled and press SET AS FULL . • After ...

33 Leica ASP300 Fig. 16 → → Adding molten paraffin • Molten wax may have a maximum temperature of 70 ° C. • If filling with molten wax, do not fill above the MAX level (see Fig. 15 ). • After a wax bath has been filled, it must be marked as ‘full’ in the REAGENT STATUS screen. Software control • If ...

34 Instruction manual V 1.3 – 12/2003 5.2 Setting up the instrument parameters From the start screen, press MENU to go to the MENU FUNCTIONS screen, then press MORE . In the MORE MENU FUNCTIONS screen, press SYSTEM SETUP . 5.2.1 System setup → 5. Operation S Y S T E M S E T U P s c r e e n (Fig. 17)...

36 Instruction manual V 1.3 – 12/2003 Security To determine the level of access to the instrument. • SUPERVISOR PASSWORD : Enabled or Disabled Enabled - A password is required for access to the supervisorlevel. Disabled - All instrument and software functions are fullyaccessible to all staff. • LOCK...

42 Instruction manual V 1.3 – 12/2003 5. Operation Fig. 21 Editing program steps • To edit a step, highlight the corresponding line and press the re-spective headline. • In the entry windows that pop up enter / select the program step set-tings. ↓ ↓ ↓ ↓ ↓

5. Operation Access the RUN OPTIONS function to display the options selected for the current program. • LEVEL CHECK enabled: The lower level sensor in the retort is acti-vated. • RECIRCULATION enabled: During each processing step, the reagentis pumped periodically from the bottle to theretort and ba...

52 Instruction Manual V 1.3 – 12/2003 → 5.4 Reagent status In the reagent status screen ( Fig. 32 ) you can display and update the actual reagent status of each station and you can set the stationsas full or empty. Fig. 32 • Warning thresholds that triggerwarning messages appear on ared background. ...

53 Leica ASP300 5. Operation 5.5 System Monitor The SYSTEM MONITOR screen supplies important infor- mation on the Leica ASP300 system status. After replacing the filter press RESET . ROTARY VALVE RETRIES: If a figure higher than 50 is indicated, a preventive maintenance isrecommended. FILL/DRAIN: Th...

56 Instruction manual V 1.3 – 12/2003 6.1 General The Leica ASP300 is equipped with a trouble shooting system. If an error is detected, detailed error messages and corresponding userinstructions on what to do are displayed on the touch screen. For all correctable error conditions the instrument will...

58 Instruction manual V 1.3 – 12/2003 6.4 Typical fill or drain problems Fill and drain problems may be due to several causes: 1. There is insufficient reagent • Verify that both the reagent bottles and the wax stations havesufficient reagent. 2. Insufficient pressure or vacuum The Leica ASP300 is u...

59 Leica ASP300 7. Cleaning and maintenance 7.1 Clean programs You find the clean programs at the end of the programslist ( Fig. 35 ). To display a clean program, highlight the re- spective line and press EDIT . The ASP300 offers three retort clean programs and one paraffinclean program. Clean progr...

60 Instruction manual V 1.3 – 12/2003 Editing steps • To edit a program step highlight the corresponding line and pressthe respective table header. • To disable a step, set the number of cycles to “ 0 “. Only cleaning wa- ter steps can be disabled. The following characteristics of each step can be e...

63 Leica ASP300 7.2 General cleaning steps Paraffin wax baths Warning! Be careful as the walls of the wax baths are very hotand may cause burns! • Wipe the wax baths and lids clean. The lid can be removed for cleaning purposes. • If they are dirty, remove the wax strainers from the wax baths. Clean,...

67 Leica ASP300 7. Cleaning and maintenance Maintenance activity daily weekly monthly every 4 months Lubricate reagent bottle O-rings √ √ ∗ and check for damage. Wipe dry retort and lid √ after completing retort clean cycle. Clean exterior of instrument √ with soft cloth and minimalamount of xylene....

69 Leica ASP300 8. Warranty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractualproduct delivered has been subjected to a comprehensive quality con-trol procedure based on the Leica in-house testing standards, and thatthe product is faultless and complies with all te...

8 Instruction manual V 1.3 – 12/2003 2. Safety 2.2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basisfor accident prevention.Primarily responsible for accident-free operation is above all the institution which owns theinstrument and, in addition, t...

9 Leica ASP300 Warnings - working with the instrument The Leica ASP300 may only be operated by trained laboratory personnel and as per thepresent instruction manual. In case of emergency switch off mains (item no. 5 in Fig. 3) at the rear of the instrument andunplug the power cord.The instrument mus...

10 Instruction manual V 1.3 – 12/2003 2.3 Integrated safety devices The instrument is equipped with the following protection features: • overpressure protection, • protection against excessive vacuum, • overcurrent protection, • overheating protection. Overpressure protection The design of the press...

11 Leica ASP300 3. Instrument components and specifications 3.1 Specific instrument options • R eagent M anagement S ystem (RMS). • The specimen cassette baskets may be used with or without cas-sette dividers and spring separators designed to separate cassettesand improve fluid penetration. • In-pro...

13 Leica ASP300 Overview - Instrument components 1 - Basic instrument - processor module 2 - Basic instrument - reagent module 3 - Wax bath lids 4 - Retort lid 5 - Touch screen 6 - Cover flap of instrument console 7 - Instrument console comprising: 8 - Printer port 9 - Local alarm connection 1 0 - R...

17 Leica ASP300 3. Instrument components and specifications 3.4 Technical Data (continued) Hardware and Software: • Large color liquid crystal display (LCD) and touchscreen. • User-friendly, intelligent software. • 3,5“ floppy drive and printer port. • Alarm system with two remote alarm sockets. • P...

18 Instruction manual V 1.3 – 12/2003 3.5 Compatible reagents Fixatives 1. Formalin, buffered or unbuffered2. Formalin saline Dehydration 1. Ethanol 4. Butanol 2. Isopropanol 5. Industrial Methylated Spirits 3. Methanol Clearing 1. Xylen (recommended)2. Toluol * 3. Chloroform * Paraffin wax 1. Paraf...

19 Leica ASP300 Moving the instrument The instrument must be installed such that the mains switch atthe rear of the instrument (item no. 5 in Fig. 3) can easily be ac-cessed at any time. After the instrument has been unpacked (see unpacking instructionsattached to outside of transport crate) it must...

20 Instruction manual V 1.3 – 12/2003 4. Setting up the instrument • Connect mains cable ( 6 ) to mains power supply socket ( 1 ) (see Fig. 3 ). • Jumper cable ( 3 ) connects the mains power supply output ( 2 ) to the electronics module input ( 4 ). • Switch ON/OFF switch ( 5 ) ON. • Switch ON/STOP ...

21 Leica ASP300 4. Setting up the instrument • Unpack the activated carbon filter and in-sert it into the instrument (see Fig. 4 ). Make sure to insert the filter with the correct sidefacing upwards. See label ( 6 ) at the front of the filter - the arrow must point upwards. • Unpack the magnetic sti...

22 Instruction Manual V 1.3 – 12/2003 4.4 Setting up the data connections Printer (optional) • Lift off the instrument console flap ( 12 ). • Connect the printer to the printer port ( 7 ) via a standard Centronics cable (refer Fig. 6 ). Other connections • For additional data transfer and storage th...

24 Instruction Manual V 1.3 – 12/2003 • After switching it on the instrument will takea few minutes to initialize (see opposite - Fig. 7 ). • After initialization, the FAVORITES screen will be displayed ( Fig. 8 ). Screen saver • A screen saver will operate turning thescreen off when no key has been...

26 Instruction manual V 1.3 – 12/2003 4.7 Touch screen functions 4.7.1 User interface - overview The Leica ASP300 is programmed and operated via a colored LCDtouch screen. An on-line help feature provides detailed information on all instrumentfunctions. HELP can be accessed from any screen by pressi...

28 Instruction manual V 1.3 – 12/2003 4. Setting up the instrument 4.8 Check list for first operation (at supervisor access level) Parameter settings E n t e r i n s t r u m e n t n a m e a n d s e l e c t l a n -guage.Activate printer (optional). Enter carbon filter age warning and pumpwarning thre...

29 Leica ASP300 5.1 Reagent handling 5.1.1 Fill/drain reagents (other than wax) 1. Manually changing the reagents • Remove the appropriate reagent bottle from the Leica ASP300 re-agent cabinet, remove the bottle screw cap. • Drain/refill the reagent by pouring to/from a bulk container. • On the REAG...

30 Instruction Manual V 1.3 – 12/2003 Select station Press key symbol Fig. 12 → 5. Operation Remote drain • On the screen, select reagent bottle no. 3 and the bulk container ( Remote ). The press STATION DRAIN . • Check for leaks. Remote fill • On the screen, select reagent bottle no. 3 and the bulk...

31 Leica ASP300 5. Operation 5.1.2 Changing the wax Draining the wax • Connect the paraffin drain hose ( 2 ) as shown in Fig. 13 and insert the loose end of the hose into a remote collecting container. Fig. 13 • Select the wax bath to be drained and theremote container (‘Remote’).Then press STATION ...

32 Instruction Manual V 1.3 – 12/2003 Fig. 14 → → • Remove the splash protector. • Fill the wax bath ( Fig. 15 ) with wax pellets up to the top. • Press BACK and REAGENT STATUS to ac- cess the reagent status table ( Fig. 14 ). High- light the wax bath you just filled and press SET AS FULL . • After ...

33 Leica ASP300 Fig. 16 → → Adding molten paraffin • Molten wax may have a maximum temperature of 70 ° C. • If filling with molten wax, do not fill above the MAX level (see Fig. 15 ). • After a wax bath has been filled, it must be marked as ‘full’ in the REAGENT STATUS screen. Software control • If ...

34 Instruction manual V 1.3 – 12/2003 5.2 Setting up the instrument parameters From the start screen, press MENU to go to the MENU FUNCTIONS screen, then press MORE . In the MORE MENU FUNCTIONS screen, press SYSTEM SETUP . 5.2.1 System setup → 5. Operation S Y S T E M S E T U P s c r e e n (Fig. 17)...

36 Instruction manual V 1.3 – 12/2003 Security To determine the level of access to the instrument. • SUPERVISOR PASSWORD : Enabled or Disabled Enabled - A password is required for access to the supervisorlevel. Disabled - All instrument and software functions are fullyaccessible to all staff. • LOCK...

42 Instruction manual V 1.3 – 12/2003 5. Operation Fig. 21 Editing program steps • To edit a step, highlight the corresponding line and press the re-spective headline. • In the entry windows that pop up enter / select the program step set-tings. ↓ ↓ ↓ ↓ ↓

5. Operation Access the RUN OPTIONS function to display the options selected for the current program. • LEVEL CHECK enabled: The lower level sensor in the retort is acti-vated. • RECIRCULATION enabled: During each processing step, the reagentis pumped periodically from the bottle to theretort and ba...

52 Instruction Manual V 1.3 – 12/2003 → 5.4 Reagent status In the reagent status screen ( Fig. 32 ) you can display and update the actual reagent status of each station and you can set the stationsas full or empty. Fig. 32 • Warning thresholds that triggerwarning messages appear on ared background. ...

53 Leica ASP300 5. Operation 5.5 System Monitor The SYSTEM MONITOR screen supplies important infor- mation on the Leica ASP300 system status. After replacing the filter press RESET . ROTARY VALVE RETRIES: If a figure higher than 50 is indicated, a preventive maintenance isrecommended. FILL/DRAIN: Th...

56 Instruction manual V 1.3 – 12/2003 6.1 General The Leica ASP300 is equipped with a trouble shooting system. If an error is detected, detailed error messages and corresponding userinstructions on what to do are displayed on the touch screen. For all correctable error conditions the instrument will...

58 Instruction manual V 1.3 – 12/2003 6.4 Typical fill or drain problems Fill and drain problems may be due to several causes: 1. There is insufficient reagent • Verify that both the reagent bottles and the wax stations havesufficient reagent. 2. Insufficient pressure or vacuum The Leica ASP300 is u...

59 Leica ASP300 7. Cleaning and maintenance 7.1 Clean programs You find the clean programs at the end of the programslist ( Fig. 35 ). To display a clean program, highlight the re- spective line and press EDIT . The ASP300 offers three retort clean programs and one paraffinclean program. Clean progr...

60 Instruction manual V 1.3 – 12/2003 Editing steps • To edit a program step highlight the corresponding line and pressthe respective table header. • To disable a step, set the number of cycles to “ 0 “. Only cleaning wa- ter steps can be disabled. The following characteristics of each step can be e...

63 Leica ASP300 7.2 General cleaning steps Paraffin wax baths Warning! Be careful as the walls of the wax baths are very hotand may cause burns! • Wipe the wax baths and lids clean. The lid can be removed for cleaning purposes. • If they are dirty, remove the wax strainers from the wax baths. Clean,...

67 Leica ASP300 7. Cleaning and maintenance Maintenance activity daily weekly monthly every 4 months Lubricate reagent bottle O-rings √ √ ∗ and check for damage. Wipe dry retort and lid √ after completing retort clean cycle. Clean exterior of instrument √ with soft cloth and minimalamount of xylene....

69 Leica ASP300 8. Warranty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractualproduct delivered has been subjected to a comprehensive quality con-trol procedure based on the Leica in-house testing standards, and thatthe product is faultless and complies with all te...