Leica ANGLE TWO- Manuals

Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 9 3 Components This chapter describes the parts of the Leica Angle Two ™ system and their functions. The components are divided into the following sections:  “U Frame” on page 10 – includes the ear bars, nose clamp and inci...

Components Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 10 3.1 U Frame Figure 4 shows the Angle Two U frame with a rat adapter and 45° ear bars attached. Some of the part names in the figures below link to sections of the manual where the part is discussed in more deta...

Components Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 11 Caution Do not use the rat incisor bar on a mouse. A mouse adaptor is available from Leica Microsystems Note: Hamsters and gerbils can use the rat ear bars and incisor bar. Nose Clamp The nose clamp is placed o...

Components Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 15 Fine Drive The fine drive (see Figure 9) provides greater control and smoother movement in the DV direction when placing or removing the probe from the brain. The fine drive helps prevent inserting the probe to...

Components Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 17 Tilt The primary tilt plane can be in either the AP or ML directions (see Figure 15). Always remember to update the software with the primary tilt direction (“Tilt Direction” on page 29). To adjust the tilt loo...

Components Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 18 2. Unlock the rotation clamp and rotate the base of the manipulator 90° in the opposite direction to the swing-out in the preceding step, so the tilt encoder is facing the front of the instrument and the probe ...

Components Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 20 3.4 Probe Shaft A clamp attaches the probe shaft to the manipulator. The clamp has two holes for positioning the clamp onto the manipulator, and you can rotate the clamp to the left or right of the manipulator ...

Components Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 21 Figure 20. The probe shaft screw Attaching a Probe At the bottom of the standard probe shaft is a plastic block with three grooved faces and one flat face. Insert the probe into one of the grooves (grooves are ...

Components Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 23 3.6 Computer The computer consists of a desktop case, monitor, mouse and keyboard. The computer comes with the Angle Two software that includes an integrated rat or mouse brain atlas. The computer should only b...

Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 24 4 Software Overview This chapter describes the Angle Two ™ software and its functions. The topics covered are:  “Atlas” on page 25 – how to select an atlas and view the coronal plates.  “Modes” on page 26 – explains wha...

Software Overview Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 28 Atlas The Atlas mode shows the exact position of the probe including the rotation, tilt and the deviation of the animal’s skull from 0° (skull flat). The Skull value is only determined once the bregma an...

Software Overview Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 29 4.3 Initial Settings Before locating the target site, you must do the following on the Angle Two software:  Select the tilt direction  Zero the tilt and rotation angles  Enter any probe offsets  Ente...

Software Overview Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 30 Enter Offsets Offsets are used when performing surgery on animals that do not fall within the weight range of the atlas being used. Offsets are a distance in millimeters that is added or subtracted from ...

Software Overview Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 31 4.4 Coordinates Coordinates can be saved or exported at any time while the instrument is being used. Double click on saved coordinates to load the target coordinates from a previous study, rather than re...

Software Overview Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 32 1. Enter a name and description. 2. In Choose Source Point select which coordinates you want to save:  Target Position – saves the current target site  Device Position – saves the current position of t...

Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 33 5 Alignment Align the instrument whenever the software is started. Alignment is necessary to check the horizontal (ML) and vertical (DV) planes are perpendicular. To align the instrument the rotation and tilt must be set ...

Alignment Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 34 5.2 Procedure The primary tilt of the instrument can be in either the ML or AP direction so there are two procedures for the vertical alignment. Select the correct instruction set for your instrument set up. Set...

Alignment Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 37 Horizontal Alignment The ML horizontal path of the probe is controlled by the rotation of the instrument. This part of the alignment procedure checks the ML horizontal path is perpendicular. To test this the dia...

Alignment Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 38 12. Use the ML drive to move the spring horizontally along the T-bar. Move the indicator to one side and then across the total length of the bar to the other side. The instrument is horizontally aligned if the d...

Alignment Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 39 AP Tilt 13. If the tilt is in the AP direction use the ML drive to position the dial indicator spring in the middle of the T-bar. Do not move the AP drive. Figure 43. Tilt (1) is in the AP direction (2) 14. Move...

Alignment Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 40 15. When the AP tilt is aligned, select ll To AP on the computer screen (see Figure 28). 16. Click Setup and click Zero Angles to zero the Tilt and Rotation . Figure 45. The set up dialog and Zero Angles button ...

Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 42 6 Using the Instrument This chapter describes the whole process of using the Angle Two ™ system to perform surgery; from starting the software to successfully placing the probe. This chapter has the following sections:  ...

Using the Instrument Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 43 Preparation 1. Ensure that the instrument is clean (see “Cleaning and Maintenance” on page 47).2. Turn the computer on and start the Angle Two software (see Figure 3).3. Align the instrument using the...

Using the Instrument Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 44 11. Support the head and insert the right ear bar into the right ear. 12. Lower the right ear bar into the instrument and lock it in place. 13. Check that both ear bar scales have the same readings. I...

Using the Instrument Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 45 Locating the Target Site 22. Click To Target (see “To Target” on page 27) to view the distances to the target site. 23. Use the ML and AP drives to position the probe directly ‘above’ the target. As t...

Using the Instrument Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 46 Probe Placement 30. Unlock the swing-out clamp and reposition the manipulator and probe over the animal.31. Relock the swing-out clamp.32. Use the coarse DV drive to approach the target site. 33. Enga...

Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 47 7 Cleaning and Maintenance Caution Do not autoclave. The bushings and probe holder block can be damaged. Caution Do not lubricate the axis rod slides or threaded drive. The plastic bushings can absorb oil and cause the in...

Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 48 8 Specifications Instrument Dimensions Resolution, Linear Scales Drive Advancement per Revolution Manipulator Movement Base width 253 mm Base length 355 mm Base height 20 mm U frame width 165 mm U frame length 200 mm U fr...

Specifications Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 49 Tooth Bar Movement Ranges Probe Holder Computer Monitor DV 20 mm AP 44.5 mm Diameter 7.88 mm Shaft length 200 mm Probe holder block 11 mm square (10 grooves spaced at 1 mm on 3 sides) Standard clamp holds 0...

Software Licence Terms Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 50 Software Licence Terms The following License Terms govern your use of the accompanying software unless you have a separate signed agreement with Leica Microsystems. License Grant Leica Microsystems ...