Page 3 - Table of Contents
TW-357 REV E 3 Table of Contents WARNING 4 Safety Precautions for Liquid Nitrogen 4 GENERAL INFORMATION 6 Text Format Notation 6 LABS Cryostorage Specifications 7 INSTALLATION 9 Unpacking and Inspection 9 Liquid Nitrogen Supply Connection 9 Power Supply Connection 10 KRYOS Control Field Installation...
Page 4 - Extreme Cold - Cover Eyes and Exposed Skin
TW-357 REV E 4 WARNING The following safety precautions are for your protection. Before installing, operating, or maintaining this unit read and follow all safety precautions in this section and in reference publications. Failure to observe all safety precautions can result in property damage, perso...
Page 6 - GENERAL INFORMATION; Menu is indicated in; ALL CAPS BOLD; Actual Menu Choices are indicated in ALL CAPS.
TW-357 REV E 6 GENERAL INFORMATION Thank you for purchasing this product. This state of the art CRYOSTORAGE SYSTEM can control either the liquid level and/or the vapor temperature range. The features are designed to provide a safe environment for samples while at the same time tracking all relevant ...
Page 7 - LABS Cryostorage Specifications; Dimensions
TW-357 REV E 7 LABS Cryostorage Specifications Liquid nitrogen at atmospheric pressure weighs 1.78 lb./liter (0.8 kg/liter). All Taylor-Wharton Gas Equipment cryostorage systems are designed to support the full weight of liquid nitrogen and a complete stainless steel or aluminum inventory control sy...
Page 8 - KRYOS Specifications
TW-357 REV E 8 KRYOS Specifications Configurations: Designed exclusively for all Taylor-Wharton LABS CryoStorage Systems Power Supply: 16.5 VAC, 40 Voltamps – Standard Battery Backup Version 24 VAC, 40 Voltamps (No Battery) (For power outlets other than North American 110 VAC contact Taylor-Wharton ...
Page 9 - Repackaging for Shipment; Liquid Nitrogen Supply Connection
TW-357 REV E 9 INSTALLATION Unpacking and Inspection Inspect shipping containers for external damage. All claims for damage (apparent or concealed) or partial loss of shipment must be made in writing within five (5) days from receipt of goods. If damage or loss is apparent, please notify the shippin...
Page 12 - Control Components; Interface Panel; Main Control; Sensor Assembly
TW-357 REV E 12 Control Components Interface Panel The KRYOS Interface panel, which the user will interact with, contains the vacuum fluorescent display as well as the number keypad, power button, help button and the soft-key control buttons. (See figure 1.0 below.) Figure 1.0 KRYOS Interface Panel ...
Page 13 - Lid Switch
TW-357 REV E 13 Lid Switch Is attached to the hinge and determines whether or not the lid is open on the freezer. This also allows the control to determine whether to activate the Quickchill, Auto Defog or Lid Alarm features. Lid Switch Figure 3.0 Lid Switch Solenoid Valve Designed to work with 12 V...
Page 14 - Operating Parameters; Liquid Phase Storage; Allow the unit to fill to your desired “Stop Fill” elevation.; MENU; Dress wires and seal with stopper.
TW-357 REV E 14 Operating Parameters When materials are immersed in liquid nitrogen, they will assume the temperature of the liquid -196ºC (-320ºF). When material is stored in the vapor phase over the liquid, the liquid nitrogen is still a very cold refrigerant, but the refrigerator’s interior tempe...
Page 15 - Thermocouple Positioning
TW-357 REV E 15 Thermocouple Positioning The thermocouple is a separate sensor used to monitor and control the temperature within the refrigerator. The factory-supplied thermocouple in the LABS cryostorage system is positioned to monitor the temperature 30” above the tray. Adding an Inventory Contro...
Page 16 - Inventory Control System Layouts (LABS Cryostorage System); LABS-40K
TW-357 REV E 16 Inventory Control System Layouts (LABS Cryostorage System) Typical 13-2 Rack 100 Vial Polycarbonate 100 Vial Aluminum Boxes 81 Vial Polycarbonate, Cardboard, or Stainless Steel Boxes LABS-20K NOTE: RACKS ARE NOT SQUARE. ORIENTATION IS IMPORTANT. LABS-80K LABS-40K 2ml vial storage – a...
Page 17 - PART NUMBER
TW-357 REV E 17 Blood Bag Cassette Blood Bag Storage in Cassette LABS-20K LABS-80K LABS-40K Fenwal 4R9951 Blood Bags – ask about other Bag types PART NUMBER CAPACITY FRAMES CASSETTES LABS-80K 2100 300 2100 LABS-40K 1204 172 1204 LABS-20K 560 80 560 Figure 6.0 Fenwal 4R59951 Blood Bag Rack Arrangemen...
Page 20 - Introduction; “alarm”
TW-357 REV E 20 Control Operation This section of the operating manual is for Taylor-Wharton approved equipment that uses the KRYOS controller. Introduction The KRYOS temperature and LN2 level controller is designed for easy operation and uninterrupted, reliable service. This controller will maintai...
Page 24 - The sensor type can be set through the menu system (; The High Temperature Alarm for Thermocouple #1; can be set through the menu system (
TW-357 REV E 24 Sensor Type The sensor type can be set through the menu system ( MENU , 2, 4). The sensor type selection should match the sensor type that is being used in the system. This is a 4-sensor, a 7-sensor (Freeze-Guard) or an 8-sensor array. If the sensor assembly is unplugged and the main...
Page 26 - DUMP ERROR LOGS
TW-357 REV E 26 The control consolidates the events in the internal log (combining “start event” / ”conclude event” information to provide one event with duration), however, when the data is sent out the serial port no consolidation of data occurs. As an example, a fill would provide one log entry i...
Page 29 - can be changed through the menu system. (; set at the factory to Central Time, USA.; , 4, 3) track all personnel who enter the freezer by asking for an
TW-357 REV E 29 Menu Password When the menu password is active, it is possible to review all of the control settings without having the password. When a password is activated and the user presses the menu key, two choices are available: 1) MENU (PASSWORD REQ) 2) CONTROL SETTINGS By selecting choice ...
Page 31 - can be set through the menu system of the control (; can be accessed in the menu system (; Control On Battery Backup Version; 4 VAC solenoid; Operation
TW-357 REV E 31 Display Brightness DISPLAY BRIGHTNESS can be set through the menu system of the control ( MENU , 6, 3). This option changes the intensity of the display. The possible choices are 25%, 50%, 75% and 100%. About this Control ABOUT THIS CONTROL can be accessed in the menu system ( MENU ,...
Page 32 - Battery Life; ABOUT THIS CONTROL
TW-357 REV E 32 The DC solenoid valve requires that the battery be connected for the system to work properly. If the battery is disconnected or completely discharged the valve operation and the control operation may be affected. In particular, if the control is calling for a fill, the solenoid will ...
Page 33 - Care
TW-357 REV E 33 MAINTENANCE Strainer Cleaning Instructions The container will not fill properly if the strainer is clogged with ice or dirt. To clean the strainer, first close the supply valve to the refrigerator. Vent the fill line of all pressure. Remove and warm the strainer to ambient temperatur...
Page 34 - Cleaning Your Taylor-Wharton CryoStorage System
TW-357 REV E 34 Cleaning Your Taylor-Wharton CryoStorage System The cryogenic vessel of all LABS CryoStorage Systems may need to be cleaned, and sterilized if the type of stored product is changed or the unit is taken out of service. The vessel must be defrosted, cleaned, and sterilized regardless o...
Page 35 - Making Adjustments to the LABS KRYOS Sensor Assembly; • Start Fill Sensor
TW-357 REV E 35 WARNING: Never use chlorine-based disinfectants to clean a LABS CryoStorage System. Normal Evaporation Rate (NER) Test Nitrogen consumption is an accumulation of all system components and user introduced evaporation. The storage chamber is a double walled, vacuum insulated vessel and...
Page 37 - Removing/Installing the Solenoid Valve; Controller Electrical Tests; Resistance greater than 50K ohms, or less that 5 ohms.
TW-357 REV E 37 • Move the sensor up or down so that the appropriate numbers of sensors are in liquid (read “L”). • Get back to the KRYOS main screen and the level indicated should match the physically measured reading. The Sensor Offset, the Start Fill and the Stop Fill can all be set through the K...
Page 38 - Sensor Plug Schematic
TW-357 REV E 38 Sensor Plug Schematic Figure 9.0 Pin View Schematics
Page 39 - LABS Plumbing Assembly
TW-357 REV E 39 LABS Plumbing Assembly Solenoid Valve Freeze-Guard Sensor Male Branch Tee 90 deg Elbow 90 deg Elbow Male Branch Tee Reducer Relief Valve Tube Strainer Female Connector Relief Valve Figure 10.0 Plumbing Assembly
Page 40 - Determine actual liquid level using a dipstick. Select; Mark the sensor assembly at top of tube to assure re-assembly.
TW-357 REV E 40 TROUBLESHOOTING Symptoms The key to troubleshooting your LABS CryoStorage system is to determine which component in the system is the source of the problem. Determine if the problem is occurring in any of the following subsystems: Supply Vessel, Transfer Line, Power Source, Temperatu...
Page 41 - Thermocouple is not repairable. Replace from stock.; Temperature reading 10 to 20 degrees warm
TW-357 REV E 41 • Simulate an over fill by lowering the next thermistor into the LN2 . HIGH LEVEL alarm should sound with 10 seconds. • Re-install sensor assembly and thermocouple into sensor tube as before. Open supply valve on LN2 supply. Indicates Low LN2 Supply When the START FILL thermistor is ...
Page 42 - Fill Solenoid Cycles On and Off; Select; To activate or de-activate this feature: Select; Access control board and allow time to fully dry.
TW-357 REV E 42 Fill Solenoid Cycles On and Off KRYOS features a Freeze-Guard function whereby the valve open duration is limited, allowed to shut for a short warm-up and then opened again. To confirm or change the valve open duration: • Select MENU , USER OPTIONS, CONTROL OPTIONS, FREEZE-GUARD OPTI...
Page 43 - Check all ribbon cable connectors on back of display panel.; this exceptionally long initial fill.
TW-357 REV E 43 Defog Feature Will Not Operate See LID OPEN ALARM. To activate or adjust times: Select MENU , USER OPTIONS, CONTROL OPTIONS, LID/DEFOG SETTINGS, AUTO DE-FOG. Push Buttons Will Not Respond 1. Check all ribbon cable connectors on back of display panel. 2. Moisture may have migrated int...
Page 44 - Factory Settings
TW-357 REV E 44 Factory Settings To restore factory settings at any time KRYOS can be re-initialized. Please note that a re-initialization clears all logs and passwords and resets settings to those listed in the table below. To reset factory settings in the control, go to the menu choice ABOUT THIS ...
Page 45 - REPLACEMENT PARTS; Parts; Controller KRYOS (Battery Backup)...................................................
45 REPLACEMENT PARTS Refrigerator Parts (LABS) 80K/40K/20K Lid Assembly……………………………………………………………LA80-9C85/LA40-9C85/LA20-9C85 Caster, ……………….. …………………………………………………………… 7300-8040 Decal, Warning……………………………………………………………………. R23K-9C42 Decal, Warning………………………………………………………………… R10K-9C42 Inventory Rack 25 Vials...
Page 47 - ©2002 Harsco Corporation
47 4075 Hamilton Blvd. Theodore, Alabama 36582 U.S.A. Telephone (251) 443-8680 Fax (251) 443-2250 In U.S. and Canada: (800) TW TANKS (898-2657) ©2002 Harsco Corporation