Leica NCL-TSH - Manual
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Leica Biosystems Newcastle Ltd
Balliol Business Park West
Benton Lane
Newcastle Upon Tyne NE12 8EW
United Kingdom
(
+44 191 215 4242
Novocastra
™
Lyophilized
Mouse Monoclonal Antibody
Thyroid Stimulating Hormone
Product Code: NCL-TSH
Intended Use
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Specificity
Thyroid stimulating hormone (TSH). No reactivity with luteinising hormone, follicle stimulating
hormone and human chorionic gonadotrophin.
Clone
QB2/6
Ig Class
IgG1
Antigen Used for
Immunizations
TSH molecule.
Hybridoma Partner
Mouse myeloma (NS0).
Preparation
Lyophilized tissue culture supernatant containing sodium azide.
Reconstitute with the volume of sterile distilled water indicated on the vial label.
Effective on Frozen Tissue
Yes.
Effective on Paraffin Wax
Embedded Tissue
Yes.
Recommendations on Use
Immunohistochemistry on paraffin sections.
Enzyme Induced Epitope Retrieval (EIER):
Please follow the instructions for use in Novocastra
Enzyme Proteinase K (IHC).
Suggested dilution:
1:100 for 30 minutes at 25 °C. This is provided as a guide and users should
determine their own optimal working dilutions.
Visualization
: Please follow the instructions for use in the Novolink
™
Polymer Detection Systems.
For further product information or support, contact your local distributor or regional office of Leica
Biosystems, or alternatively, visit the Leica Biosystems Web site, www.LeicaBiosystems.com
The performance of this antibody should be validated when utilized with other manual staining
systems or automated platforms.
Positive Controls
Immunohistochemistry: Normal human pituitary gland.
Staining Pattern
Cytoplasmic
Storage and Stability
Store unopened lyophilized antibody at 2-8 °C. Under these conditions, there is no significant
loss in product performance up to the expiry date indicated on the vial label. The reconstituted
antibody is stable for at least two months when stored at 2-8 °C. For long term storage, it is
recommended that aliquots of the antibody are frozen at -20 °C (frost-free freezers are not
recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on
the day of use.
Warnings and Precautions
This reagent has been prepared from the supernatant of cell culture. As it is a biological product,
reasonable care should be taken when handling it. This reagent contains sodium azide. A Material
Safety Data Sheet is available upon request or available from www.LeicaBiosystems.com
TSH 27/07/2012
© Leica Biosystems Newcastle Ltd • NCL- Rev C
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