Leica NCL-L-ALK - Manual
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NCL-L Rev D
Novocastra
™
Liquid
Mouse Monoclonal Antibody
p80 (Anaplastic Lymphoma Kinase)
Product Code: NCL-L-ALK
Intended Use
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Specificity
Human ALK protein and C-terminal ALK fusion proteins containing the immuized target sequence.
Clone
5A4
Ig Class
IgG1
Antigen Used for
Immunizations
Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase
catalytic domain and part of the C-terminus of the NPM-ALK transcript (419-520 amino acids).
Hybridoma Partner
Mouse myeloma (p3-NS1-Ag4-1).
Preparation
Liquid tissue culture supernatant containing sodium azide.
Volume as indicated on vial label.
Effective on Frozen Tissue
Not evaluated
Effective on Paraffin Wax
Embedded Tissue
Yes
Recommendations on Use
Immunohistochemistry on paraffin sections.
Heat Induced Epitope Retrieval (HIER):
Please follow the instructions for use in Novocastra
Epitope Retrieval Solution pH 6.
Suggested dilution
: 1:200 for 30 minutes at 25 °C. This is provided as a guide and users should
determine their own optimal working dilutions.
Visualization
: Please follow the instructions for use in the Novolink
™
Polymer Detection Systems.
For further product information or support, contact your local distributor or regional office of Leica
Biosystems, or alternatively, visit the Leica Biosystems web site, www.LeicaBiosystems.com
The performance of this antibody should be validated when utilized with other manual staining
systems or automated platforms.
Western Blotting:
Not recommended
Positive Controls
Immunohistochemistry: a proportion of anaplastic large cell lymphomas.
Staining Pattern
Cytoplasmic and/or nuclear.
Storage and Stability
Store at 2–8 °C. Do not freeze. Return to 2–8 °C immediately after use. Do not use after
expiration date indicated on the vial label. Storage conditions other than those specified above
must be verified by the user.
Warnings and Precautions
This reagent has been prepared from the supernatant of cell culture. As it is a biological product,
reasonable care should be taken when handling it.
This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or
available from www.LeicaBiosystems.com
General Overview
p80 is encoded by a hybrid gene that occurs as a result of the chromosomal translocation, t(2;5)
(p23;q35). The protein consists of the amino terminus of the nucleophosmin (NPM) gene fused
to the catalytic domain of anaplastic lymphoma kinase (ALK). Full-length ALK is a 1620 amino
acid protein that exhibits 57% amino acid identity to leukocyte tyrosine kinase (LTK). The mature
form of ALK is a 200 kD glycosylated single chain molecule that occurs on the surface of cell
membranes and probably acts as a ligand receptor.
ALK-L 13/12/2012
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