Leica NCL-CK17 - Manual
Leica NCL-CK17 – Manual, read for free online in PDF format. We hope this helps you resolve any issues you may have. If you have further questions, please contact us through the contact form.
Leica Biosystems Newcastle Ltd
Balliol Business Park West
Benton Lane
Newcastle Upon Tyne NE12 8EW
United Kingdom
(
+44 191 215 4242
© Leica Biosystems Newcastle Ltd
•
NCL- Rev D CK17 21/01/2014
Novocastra
TM
Lyophilized
Mouse Monoclonal
Antibody
Cytokeratin 17
Product Code: NCL-CK17
Intended Use
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Specificity
Human cytokeratin 17 intermediate filament protein.
Clone
E3
Ig Class
IgG2b
Antigen Used for
Immunizations
Keratin preparation from rat enterocytes.
Hybridoma Partner
Mouse myeloma (X63-Ag8.653).
Preparation
Lyophilized tissue culture supernatant containing sodium azide.
Reconstitute with the volume of sterile distilled water indicated on the vial label.
Effective on Frozen Tissue
Yes
Effective on Paraffin Wax
Embedded Tissue
Yes
Recommendations on Use
Immunohistochemistry on paraffin sections.
Heat Induced Epitope Retrieval (HIER):
Please follow the instructions for use in Novocastra
Epitope Retrieval Solution pH6.
Suggested dilution:
1:20 for 30 minutes at 25 °C. This is provided as a guide and users should
determine their own optimal working dilutions.
Visualization:
Please follow the instructions for use in the Novolink™ Polymer Detection Systems.
For further product information or support, contact your local distributor or regional office of Leica
Biosystems, or alternatively, visit the Leica Biosystems Web site, www.LeicaBiosystems.com
The performance of this antibody should be validated when utilized with other manual staining
systems or automated platforms
.
Positive Controls
Immunohistochemistry: Trachea.
Western Blotting: HeLa cell line.
Staining Pattern
Cytoplasmic.
Storage and Stability
Store unopened antibody at 2–8 °C. Under these conditions, there is no significant loss in product
performance up to the expiry date indicated on the vial label. Do not use after expiration date
indicated on the vial label. The reconstituted antibody is stable for at least two months when
stored at 2–8 °C. For long term storage, it is recommended that aliquots of the reconstituted
antibody are stored frozen at -20 °C (frost-free freezers are not recommended). Repeated
freezing and thawing must be avoided. Prepare working dilutions on the day of use. Return to 2–8
°C immediately after use. Storage conditions other than those specified above must be verified
by the user.
Warnings and Precautions
This reagent has been prepared from the supernatant of cell culture confirm. As it is a biological
product, reasonable care should be taken when handling it. This reagent contains sodium azide.
A Material Safety Data Sheet is available upon request or available from
www.LeicaBiosystems.com
"Loading the manual" means you need to wait until the file loads and becomes available for online reading. Some manuals are very large, and the time they take to appear depends on your internet speed.
