Leica NCL-CEA-2 - Manual
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NCL- Rev D CEA-2 12/01/2014
Novocastra
TM
Lyophilized
Mouse Monoclonal
Antibody
Carcinoembryonic Antigen (CD66e)
Product Code: NCL-CEA-2
Intended Use
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Specificity
Human carcinoembryonic antigen (CD66e).
Clone
12-140-10
Ig Class
IgG1
Antigen Used for
Immunizations
CEA isolated from liver metastasis of colorectal carcinomas by PCA extraction followed by ion
exchange and gel filtration chromatography.
Hybridoma Partner
Mouse myeloma (X63-Ag8).
Preparation
Lyophilized tissue culture supernatant containing sodium azide.
Reconstitute with the volume of sterile distilled water indicated on the vial label.
Effective on Frozen Tissue
Yes
Effective on Paraffin Wax
Embedded Tissue
Yes
Recommendations on Use
Immunohistochemistry on paraffin sections.
Enzyme Induced Epitope Retrieval (EIER):
Please follow the instructions for use in Novocastra
Enzyme Proteinase K (IHC).
Suggested dilution:
1:200 for 30 minutes at 25 °C. This is provided as a guide and users should
determine their own optimal working dilutions.
Visualization:
Please follow the instructions for use in the Novolink™ Polymer Detection Systems.
For further product information or support, contact your local distributor or regional office of Leica
Biosystems, or alternatively, visit the Leica Biosystems Web site, www.LeicaBiosystems.com
The performance of this antibody should be validated when utilized with other manual staining
systems or automated platforms.
Positive Controls
Immunohistochemistry: Colon.
Western Blotting: Not evaluated.
Staining Pattern
Cytoplasmic and lumenal membrane.
Warnings and Precautions
This reagent has been prepared from the supernatant of cell culture confirm. As it is a biological
product, reasonable care should be taken when handling it. This reagent contains sodium azide.
A Material Safety Data Sheet is available upon request or available from
www.LeicaBiosystems.com
Storage and Stability
Store unopened antibody at 2–8 °C. Under these conditions, there is no significant loss in product
performance up to the expiry date indicated on the vial label. Do not use after expiration date
indicated on the vial label. The reconstituted antibody is stable for at least two months when
stored at 2–8 °C. For long term storage, it is recommended that aliquots of the reconstituted
antibody are stored frozen at -20 °C (frost-free freezers are not recommended). Repeated
freezing and thawing must be avoided. Prepare working dilutions on the day of use. Return to
2–8 °C immediately after use. Storage conditions other than those specified above must be
verified by the user.
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