Page 2 - with; for excellent contrast.
Leica MZ16 F – User manual 2 2 Leica MZ16 F – User manual Dear User, We thank you for your faith in our product, andhope it brings you both enjoyment and success. With the new fully apochromatic Leica MZ16 Ffrom Leica Microsystems, you have purchased thebest-performing, manual fluorescence stereomi-...
Page 3 - Operating controls
3 Leica MZ16 F – User manual Operating controls 1 Zoom magnification changer 2 Coarse/fine focusing drive 3 Double iris diaphragm 4 Rapid filter changer for four selectable filter sets 5 Filter holder for an individually selectable filter 6 Switch for activating/deactivating theUV shutter 7 Trinocul...
Page 4 - Contents
Page 4. Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 4.1 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 4.1.1 Fluorescence technology . . . . . . . . . . .18 4.1.2 Functional principle . . . . . . . . . . . . . . . .18 5. Functions . . . . . . . . ....
Page 5 - – User Manual for ebq 100 isolated; Before installing, operating or using the
Leica MZ16 F – User manual 5 Leica MZ16 F – Safety concept 5 1. Safety concept 1.1 The user manual Included with your Leica MZ16 F fluorescencestereomicroscope is an interactive CD-ROM withall pertinent user instructions in German, English,French, Spanish, Italian, Portuguese, Dutch, Dan-ish, Swedis...
Page 6 - Safety instructions
6 Leica MZ16 F – Safety concept 1.1.2 Symbols used Warning of a danger This symbol indicates especially important infor-mation that, if not observed,– can cause hazards to personnel,– can lead to functional disturbances and dam- aged instruments. Warning of hazardous electrical voltage This symbol i...
Page 7 - Permitted uses
Leica MZ16 F – User manual 7 Leica MZ16 F – Safety concept Permitted uses With the Leica MZ16 F fluorescence stereomicro-scope, entire fluorescent specimens can beexamined three-dimensionally and unprepared,manipulated, sorted and documented. The sepa-rate beam path for the fluorescence illumination...
Page 9 - Precautions by the manufacturer
Leica MZ16 F – Safety concept 9 1.3 Light source: safety instructions Precautions by the manufacturer – UV protection screen in front of the specimen plane prevents the user from looking directlyinto the UV rays. – Simple filter holders (that have only two open- ings for the observation beam paths) ...
Page 10 - Overview of the instrument
10 Leica MZ16 F – Overview of the instrument 2. Overview of the instrument 1 Stand (transmitted or incident light stand withcorresponding illumination). Pictured here isthe HL RC™ transmitted-light base for bestrelief contrast. 2 Focusing drive: (pictured) manual coarse/finedrive with column (see Us...
Page 11 - and Leica DFC300 FX digital camera for fluorescence
Leica MZ16 F – Overview of the instrument 11 7 Filter sets: the scope of delivery includes threesimple filter holders (which have only twoopenings for the observation beam paths) forempty filter positions and transmitted or inci-dent light observation without fluorescence.Additionally, various filte...
Page 12 - Assembly sequence; filter, where applicable
12 Leica MZ16 F – Assembly 3. Assembly Basic components such as the stand, column,microscope carrier, binocular tubes and opticalaccessories are, thanks to their modular design,identical for all Leica M series stereomicro-scopes. Therefore, for assembly instructions,please refer to the User Manual f...
Page 14 - Assembly of stereomicroscope with
14 Leica MZ16 F – Assembly 3.2 Assembly of stereomicroscope with stand Do not yet connect the peripherals to the power supply! Focusing drive ➔ stand base 왘 Fasten the manual or motorized focusing drive(4.2) to the column according to User ManualM2-105-0 (manual) or M2-267-1 (motorized) onthe base (...
Page 15 - Additional components; Please read
Leica MZ16 F – Assembly 15 3.3 Additional components 왘 Fasten the other components and other individ-ual accessories according to User Manual M2-105-0: – Insert the binocular tube (2.5) or the video/ phototube into the dovetail ring on the FLUOIII ® filter system and fasten it using a clamping screw...
Page 16 - Always position the UV protection screen; Equipping the filter changer; The patented FLUOIII; If you are using fewer than four filter hold-; UV protection screen
16 Leica MZ16 F – User manual Leica MZ16 F – Assembly 3.5 UV protection screen ➔ microscope carrier 왘 Fasten the UV protection screen (5.1) using ahexagon-head screw (5.3) to the left or rightside of the microscope carrier. Tool: Allen key. Always position the UV protection screen so that the operat...
Page 17 - To avoid overheating the filter holder, do
Leica MZ16 F – User manual 17 Leica MZ16 F – Assembly 1 2 3.6.2 Simple filter holders Three simple filter holders are included in thescope of delivery of the Leica MZ16 F. These filterholders have two empty openings for the observa-tion beam paths. The third opening, to the illumi-nation beam path, ...
Page 18 - tion and the patented FLUOIII; Stereomicroscope settings and; We recommend that you first familiarize; After switching on the high-pressure; other patents applied for.
18 Leica MZ16 F – Operation 4. Operation 4.1 Description 4.1.1 Fluorescence technology Fluorescent substances light up (fluoresce) when irradiated with short-wave excitation light. Fluo-rescence technology makes use of this propertyby coloring or marking certain object structuresand details that do ...
Page 20 - Rapid filter changer; Commissioning the fluorescence; After switching on the high-pressure mer-
20 Leica MZ16 F – Functions 5.3 Rapid filter changer The rapid filter changer must be equipped with four filter holders at all times. If you are using fewer than four filter hold- ers with fluorescence filters, always insert theincluded simple filter holders into the availablepositions. Otherwise th...
Page 21 - • This provides a better overview and allows you
Leica MZ16 F – Work flow 21 6. Work flow 왘 Switch on the supply unit (page 15). Wait for 2 to 3 minutes. 왘 Close the illumination beam path using theswitch (10.1). 왘 Correctly set the binocular tube, eyepieces andworking distance in accordance with the UserManual M2-105-0. 왘 Observe the specimen at ...
Page 22 - Filter sets
22 Leica MZ16 F – Appendix 7. Appendix 7.1 Filter sets Filter sets Excitation filter Barrier filter Designation GFP 425/60 nm (395–455 nm) 480 LP GFP1 GFP Plus 480/40 nm (460–500 nm) 510 LP GFP2 GFP Plants 470/40 nm (450–490 nm) 525/50 nm (500–550 nm) GFP3 UV 360/40 nm (340–380 nm) 420 LP UV Violet ...
Page 23 - Optics carrier
Leica MZ16 F – Appendix 23 7.2 Technical data Optics carrier Leica MZ16 F Microscope type stereo fluorescence microscope with apochromatic optics system, patented TripleBeam ® third beam path, patented FLUOIII ® fluorescence filter system, and manual functions 100% apochromatic CMO (Common Main Obje...
Page 24 - Optical Data
24 Leica MZ16 F – Appendix Leica MZ16 F Optical Data Zoom 16:1 manual, apochromatic Selectable zoom positions Click-stops at 0.71 | 1 | 1.6 | 2 | 2.5 | 3.2 | 4 | 5 | 6.3 | 8 | 10 | 11.5 for repetitive tasksPerformance with standard optics(1 × objective/10 × eyepieces) – Zoom range 7.1 × – 115 × – Re...
Page 25 - Various accessories
Leica MZ16 F – Appendix 25 Stands, illuminators Leica MZ16 F Transmitted light stand HL RC™ high-performance base with innovative contrast meth-ods, stands for bright field and bright/dark field Incident light stand With stage plate, black/white Stand for large objects Swinging arm stand, stage clam...
Page 26 - Technical Data
26 Leica MZ16 F – Appendix Leica MZ16 F Technical Data Weights – Leica MZ16 F optics carrier– Lamp housing 2.5 kg – Filter set 20 g – Planapochromatic objective 1 × 0.74 kg – Focusing drive, coarse/fine, with column 1.15 kg– Microscope carrier 0.495 kg ebq 100 supply unit For indoor use only.– Suppl...
Page 27 - Eyepieces
Leica MZ16 F – User manual 27 Leica MZ16 F – Appendix 27 7.3 Optical data Field of view diameter (mm) Eyepieces Working distances in mm Objectives 0.71 28.4 8.4 14.2 16.9 17.9 13.4 22.7 10.5 45.4 5.3 56.8 4.2 0.8 32 7.5 16 15 20.5 11.7 25.6 9.4 51.2 4.7 64 3.8 1 40 6 20 12 25.6 9.4 32 7.5 64 3.8 80 ...
Page 28 - Dimensions in mm
7.4 Dimensions 28 Leica MZ16 F – Appendix Dimensions in mm
Page 30 - Stereo & Macroscope Systems
Leica Microsystems – the brandfor outstanding products Leica Microsystems – an international company with a strong network of customer services Australia: Gladesville, NSW Tel. +1 800 625 286 Fax +61 2 9817 8358 Austria: Vienna Tel. +43 1 486 80 50 0 Fax +43 1 486 80 50 30 Canada: Richmond Hill/Onta...
