Leica M205 A - Manual

Leica M series Manual 9 Table of Contents Transmitted-light Base TL BFDF: Before First Use 30 Transmitted-light Base TL BFDF 31 TL RC™ / TL RCI™ 32 IsoPro™ Manual Cross-stage: Assembly 33 IsoPro™ Motorized Cross-stage: Assembly 36 Cables: Terminals 40 Cables: Cable Duct 41 Cables: Diagram 42 Leica L...

Leica M series Manual 14 A Step Towards Infinity Ever since their introduction by Horatio S. Greenough, stereomicroscopes have worked according to the optical principles based primarily on Ernst Abbe's research. For over a century, ingenious optics designers and engi-neers have worked to push magnif...

Leica M series Manual 16 Have a special request? Let us know! Leica Microsystems enjoys an exceptional repu-tation when it comes to devising customer-specific solutions. If you have a special request that cannot be met with standard parts, contact your Leica consultant. We have a solution for every ...

Leica M series Manual 17 Leica engineers were careful to ensure that the new M series—like its predecessors—remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused. Objectives All new objectives of the M series are parfocal, meaning that when used wi...

Leica M series Manual 18 On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the instal-lation instructions and go directly to the Quick Start Guide on page 46. If, on the other hand, you are assembling the microscope...

Leica M series Manual 20 The first step is to connect the focusing column of the M series to the corresponding base. Tools used Hex socket screwdriver, 3 mm ★ Assembling the column adapter 1. Securely install the column adapter on the column using the four included screws. Assembling the focusing dr...

Leica M series Manual 21 Tools used Hex socket screwdriver, 4 mm ★ Assembling the optics carrier 1. Place the optics carrier on the focusing column so that the screw fit into the thread provided and the lug fits into the groove. 2. Press the optics carrier backwards to the focusing column and screw ...

Leica M series Manual 22 Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner. Tools used No tools required. ★ Preparations 1. Unscrew the positioning screw and remove the protective cover. Assembling the tube 2. Push the tube (for exam...

Leica M series Manual 24 Objective Tools used No tools required. ★ Preparation 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective • Hold the objective firmly during assem-bly and disassembly so that it does not fall onto the stage plate. This applies particu-la...

Leica M series Manual 25 Preparations • Hold the objectives firmly during assembly and disassembly so that they do not fall onto the stage plate. Move the drive housing all the way upwards ★ and remove the optics carrier, if the carrier has already been installed. Assembly 1. Remove the transport an...

Leica M series Manual 26 Objective Nosepiece – Adjusting Parfocality The following procedure only has to be carried out once. Afterwards, both objectives are parfo-cal, meaning that the specimen remains in focus when the objective nosepiece is rotated. This procedure must be repeated if you replace ...

Leica M series Manual 27 Tools Hex socket screwdriver, 2.5 mm ★ Preparing the AX carrier Before the AX carrier is installed, it may first have to be prepared for the optics carrier to be used (Leica M125, M205 C or M165 C). 1. Check the switch position. If the switch position corresponds to the ★ op...

Leica M series Manual 28 Tools Hex socket screwdriver, 4 mm ★ Philips screwdriver ★ Once the AX carrier has prepared for the corre-sponding optics carrier, it can be installed. 1. Move the slide of the AX carrier into mid position. 2. Install the AX carrier on the focusing column. 3. Screw the objec...

Leica M series Manual 30 Removing the transport anchors • Before you can use the transmitted-light base for the first time, it is abso- lutely necessary to remove the two transport anchors as follows. Transmitted-light Base TL BFDF: Before First Use Anchor of switching slide Anchor of mirror

Leica M series Manual 31 Transmitted-light Base TL BFDF Standard delivery The base is delivered with the adapter plate installed. The selected stage (IsoPro™ cross-stage or standard stage 10 447 269), and the focusing drive will have to be mounted later. Stage assembly The TL BFDF transmitted-light ...

Leica M series Manual 44 Under certain circumstances, the light source must not be moved along with the optics carrier. A typical example is multifocus images in which the Z-stack changes while the angle of incidence of the light must remain the same. For such purposes, the Leica LED5000 MCI™ is dir...

Leica M series Manual 47 The Fastest Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner, and naturally you want to get right to work. Therefore, your next step should be to study the Quick Start Guide, which outlines the most impor...

Leica M series Manual 48 Overview of an M series Microscope 1 Magnification changer (zoom)2 Switch click stops on/off3 Coarse/fine focusing4 Fastening screw for the binocular tube (or accessories) 5 Interchangeable objective6 Adjustable eyepiece tubes7 Eyepieces for spectacle wearers with dioptric c...

Leica M series Manual 49 The Correct Interpupillary Distance The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. If you are still a novice microscope user, you may need a short time to become accustomed to this. Not to worry—after a littl...

Leica M series Manual 50 If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups counterclockwise towards the rear, as otherwise the viewing distance is too great. 2. If an eyepiece is equipped with the inte- grated dioptric correction, turn the value to the "0" mark. By the ...

Leica M series Manual 52 Adjusting the resistance of the focus drive Adjusting the resistance Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? The resistance can be adjusted individually depend-ing on the equipment weight and personal preferences as follows: 1. ...

Leica M series Manual 54 The zoom button can optionally be operated either with or without ratchet steps. Continu-ous zoom is possible when the ratchet steps are disabled, which many users find convenient. On the other hand, when the ratchet steps are enabled, photographs, measurement results etc. c...

Leica M series Manual 55 Parfocality: More Comfort and Convenience for Your Work All Leica stereomicroscopes are parfocally matched, meaning that you can view a focused specimen from the lowest to the highest magni-fication without having to refocus. There is no need to readjust the focus to view of...

Leica M series Manual 56 The iris diaphragm in the optics carrier of your M series microscope has the same purpose as those in a camera shutter: it regulates the avail-able light, which changes the depth of field. The "depth of field" is the area of a specimen that is brought into sharp focu...

Leica M series Manual 58 Magnification Factors of the Eyepieces An eyepiece not only makes it possible to look passively into the micro-scope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x. The following eyepieces are available for the M...

Leica M series Manual 59 Health Notes Potential sources of infection • Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered separat...

Leica M series Manual 61 Adjusting 1. Set the dioptric correction for both eyepieces to "0". 2. Select the lowest magnification and focus on a flat specimen. 3. Select the highest magnification and read- just the sharpness. 4. Select the lowest magnification again, but do not look into the e...

Leica M series Manual 62 Graticules 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the grati-cule fits tightly. 3. Screw the insert and graticule firmly into place and replace the eyepiece in the tube. 4. You can now align the graticule by rotating the eyepiece in the ...

Leica M series Manual 65 Photo tubes and C-mounts Application All Leica DFC cameras are equipped with a standardized C-mount interface. In turn, the C-mount adapter for the respective trinocular tube is connected to this interface. This adapter creates a solid mechanical connection between the micro...

Leica M series Manual 69 Stereoscopic viewing A stereoscopic view is required for examining spatial objects. For this purpose, the micro-scope carrier AX must be brought to its home position. 1. Unscrew the clamping screw. 2. Push the microscope into the home posi- tion (mid position) to obtain a sp...

Leica M series Manual 71 Use The objective nosepiece enables you to switch between two objectives using just one hand movement, for example between a 1× and a 2× planapochromat. Parfocal work With the new M series objectives, the parfo-cality is maintained even when objectives are changed, meaning t...

Leica M series Manual 73 The Different Types of Objectives To meet the various requirements regarding imaging properties, there is a choice of high-quality interchangeable planachromatic and planapochromatic objectives and also lower-priced interchangeable achromatic objectives. Achromatic objective...

Leica M series Manual 77 Changing the Halogen Lamp • Before you change the lamp, it is abso-lutely necessary to unplug the power plug from the base to prevent the risk of elec-tric shock! The halogen lamp becomes very hot during operation. Therefore, to avoid being burned, let the base cool off for ...

Leica M series Manual 79 Light intensity control • Please observe the user manual—in particular, all safety regulations—from the manufacturers of the light guide and cold light source. Switch on the cold light source according to ★ the manufacturer's user manual and adjust the brightness. Transmitte...

Leica M series Manual 81 TL RCI™: The Deflection Mirror Inverted world? Depending on the properties of the specimen (refractive index of the environment) and the perception of the viewer, it is possible that the switches described below for positive and inverted relief contrast are to be operated in...

Leica M series Manual 82 TL RCI™: Color Intensity and Temperature 1 Potentiometer for controlling the CCIC™ (Constant Color Intensity Control) illumina-tion intensity 2 Potentiometer for controlling the IsoCol™ color temperature The transmitted-light base TL RCI™ has two electronic potentiometers th...

Leica M series Manual 84 TL RCI™: Methods in Transmitted Light Vertical bright-field illumination Suitable for stained amplitude specimens with sufficient contrast. The light beams are deflected vertically through the specimen. This results in an accurate bright field with maximum brightness. Inclin...

Leica M series Manual 85 TL RCI™: Relief Images Starting position 1. Push the deflection mirror all the way back towards the column. 2. Turn the deflection mirror into the notch position at an angle of 45°. Positive relief contrast Suitable for semitransparent and transparent specimens. The phase st...

Leica M series Manual 87 Filters for TL RC™ and TL RCI™ The transmitted-light bases TL RC™ and TL RCI™ can be equipped with up to three filters—available as accessories—simultane-ously. By customer request, the filters are also available as one-off items. 1. Switch off the light source or click (TL ...

Leica M series Manual 106 Specifications for the Bases

Leica M series Manual 110 IsoPro™ Motorized XY Stage Compatibility Leica incident light base and TL bases (TL BFDF, TL RC™, TL RCI™) Stage size (L × W × H) 335.5 mm × 370 mm × 41.5 mm Travel 152 mm × 102 mm (6" × 4") Speed X,Y 30 mm/s Motor resolution X,Y 0.25 µm Repeatability X, Y ± 2 µm Po...