Leica M205 FA - Manual

Leica M-series User Manual 5 Safety Instructions Description The individual modules fulfill the highest requirements for observation and documenta-tion of Leica stereomicroscopes of the M series. Intended use Refer to "Safety Concept" booklet ▶ Non-intended use Refer to "Safety Concept&#...

Leica M-series User Manual 9 Contents General Instructions 2 Important Safety Notes 3 Symbols Used 4 Safety Instructions 5 The Leica M Series for Fluorescence Congratulations! 13 A Step Towards Infinity 14 The Electronics: Comfort, Convenience and Safety 15 The Modular Design: Everything is Relative...

Leica M-series User Manual 14 A Step Towards Infinity Ever since their introduction by Horatio S. Greenough, stereomicroscopes have worked according to the optical principles based primarily on Ernst Abbe's research. For over a century, ingenious optics designers and engi-neers have worked to push m...

Leica M-series User Manual 16 Have a special request? Let us know! Leica Microsystems enjoys an exceptional repu-tation when it comes to devising customer-specific solutions. If you have a special request that cannot be met with standard parts, contact your Leica consultant. We have a solution for e...

Leica M-series User Manual 17 Leica engineers were careful to ensure that the new Leica M series—like its predecessors—remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused. Objectives All new objectives of the Leica M series are parfocal, meaning ...

Leica M-series User Manual 18 On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the instal-lation instructions and go directly to the Quick Start Guide on Page 63. If, on the other hand, you are assembling the micro...

Leica M-series User Manual 20 The first step is to connect the focusing column of the M series to the corresponding base. Tools used Hex socket screwdriver, 3 mm ρ Assembling the column adapter 1. Securely install the column adapter on the column using the four included screws. Assembling the focusi...

Leica M-series User Manual 21 Assembling the Focusing Column with an Incident-light Base When using an incident-light base, the focus-ing column and motorized focus are installed directly on the base; no extension plate is required. Tools used Hex socket screwdriver, 3 mm ρ Assembly 1. Place the foc...

Leica M-series User Manual 22   Depending on the work situation, it is useful to restrict the maximum travel path of the stereomicroscope. This prevents injury during handling of the sample caused by fingers or hands becoming trapped or the specimen touching the objective or even being damaged by it...

Leica M-series User Manual 23 Tools used Hex socket screwdriver, 4 mm ρ Assembling the optics carrier 1. Place the optics carrier on the focusing column so that the screw fits into the thread provided and the lug fits into the groove. 2. Press the optics carrier backwards to the focusing column and ...

Leica M-series User Manual 24 Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner. Tools used No tools required. ρ Preparations 1. Unscrew the positioning screw and remove the protective cover. Assembling the tube 2. Push the tube (for...

Leica M-series User Manual 25 Eyepieces Tools used No tools required. ρ Magnification range You can extend the overall magnification range using available 10×, 16×, 25× and 40× wide-field eyepieces for persons wearing glasses. Preparation 1. If you want to use an optional graticule, insert it now (P...

Leica M-series User Manual 26 Objective Tools used No tools required. ρ Preparation 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective •  Hold the objective firmly during assem-bly and disassembly so that it does not fall onto the stage plate. This applies part...

Leica M-series User Manual 27 UV Protection Screen: Assembly Tools used Allen key ρ Intended use The UV protection screen in front of the speci-men plane prevents the user from looking directly into the UV rays. Safety Notes •  UV radiation can damage the eyes. Therefore, it is mandatory to install ...

Leica M-series User Manual 28 Preparations •  Hold the objectives firmly during assembly and disassembly so that they do not fall onto the stage plate. Move the drive housing all the way upwards ρ and remove the optics carrier, if the carrier has already been installed. Assembly 1. Remove the transp...

Leica M-series User Manual 29 Objective Nosepiece – Adjusting Parfocality The following procedure only has to be carried out once. Afterwards, both objectives are parfo-cal, meaning that the specimen remains in focus when the objective nosepiece is rotated. This procedure must be repeated if you rep...

Leica M-series User Manual 30 Transmitted-light Base TL ST Unpacking the base The base is delivered with the adapter plate installed. Make sure the instruments are unpacked on a flat, sufficiently dimensioned, and non-slip surface. Focusing drive and column 1. Unscrew the extension plate from the ba...

Leica M-series User Manual 31 FluoCombi III™ – Preparations   Before assembling the FluoCombi™, you have to disconnect all instruments from the power supply. Failure to observe these instructions can result in damage to the micro-scope and the connected instruments. Preparations 1. Remove the two sc...

Leica M-series User Manual 32 FluoCombi III™ – Assembly 1. Guide the FluoCombi III™ in from below, while holding it flat. Ensure that it is aligned with the holder on the focusing column at the left and right. 2. Hold the FluoCombi III™ securely and screw it to the focusing column using the provided...

Leica M-series User Manual 34 FluoCombi III™ – Adjusting the Parfocality Setting up the objective nosepiece   The following configuration only has to be carried out once. It ensures that the parfocality remains intact, and thus the speci-men does not need to be brought into focus again. 1. Place a s...

Leica M-series User Manual 36 4. Center the objective over the specimen by loosening or tightening the screw on one side of the objective holder while turning the screw on the other side in the opposite direction. FluoCombi III™ – Centering the Objectives   The following configuration only has to be...

Leica M-series User Manual 37 FluoCombi III™ – Filter and UV Protection Screen Inserting the dichroic mirror 1. Pull the dummy filter carrier out of the holder. 2. Insert the corresponding dichroic mirror. Assembling the UV protection screen 1. Screw the UV protection screen to the holder provided o...

Leica M-series User Manual 38 Removing the transport anchors •  Before you can use the transmitted-light base for the first time, it is abso- lutely necessary to remove the two transport anchors. Transmitted-light Base TL BFDF: Before First Use Anchor of switching slide Anchor of mirror

Leica M-series User Manual 39 Transmitted-light Base TL BFDF Standard delivery The base is delivered with the adapter plate installed. The selected stage (Leica IsoPro™ mechanical stage or Leica standard stage 10 447 269), and the focusing drive will have to be mounted later. Stage assembly The Leic...

Leica M-series User Manual 41 IsoPro™ Manual Mechanical Stage: Assembly Before the Leica IsoPro™ mechanical stage is mounted to the base, the axis containing the control buttons is attached either on the left or the right side of the mechanical stage. Leica IsoPro™ Mechanical Stage Before the Leica ...

Leica M-series User Manual 44 Basics The transmitted light bases of the Leica TL series (TL BFDF, TL RC™, TL RCI™) are supplied with an installed extension plate. The selected stage (Leica IsoPro™ mechanical stage or standard stage 10 447 269) and the focusing drive will have to be mounted later. Th...

Leica M-series User Manual 52 Under certain circumstances, the light source must not be moved along with the optics carrier. A typical example is multifocus images in which the Z-stack changes while the angle of incidence of the light must remain the same. For such purposes, the Leica LED5000 MCI™ i...

Leica M-series User Manual 54 •  The manufacturer assumes no respon-sibility or liability for any use outside of the intended use or use outside of the specifica-tions from Leica Microsystems Wetzlar GmbH or any risks resulting from such use. In such cases, the Declaration of Conformity shall be inv...

Leica M-series User Manual 57 Setting up the instrument •  Set up the Leica EL6000 so that the front is readily accessible and visible. The ventilation slots on the sides and back ρ panel of the device must not be covered. Maintain a free room of at least 150 mm on ρ the rear panel of the instrument...

Leica M-series User Manual 59 Leica EL6000 – Connection to the Fluorescence Lamp Housing •  Always connect the light guide to the lamp housing of the stereomicroscope in order to prevent danger to the user from the high-energy light. 1. Unscrew the positioning screw and remove the protective cover o...

Leica M-series User Manual 61 Leica EL6000 – Replacing the Lamp The procedure for replacing the lamp does not differ from the installation procedure outlined on Page 57, except for the fact that the existing lamp has to be removed first. Replacing the lamp   The lamp used in the instrument becomes v...

Leica M-series User Manual 64 The Fastest Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner, and naturally you want to get right to work. Therefore, your next step should be to study the Quick Start Guide, which outlines the most ...

Leica M-series User Manual 65 Overview of an M Series Microscope 1 Eyepieces for spectacle wearers with dioptric correction and eyecups 2 Trinocular tube3 Fluorescence housing4 Filter Changer5 Display6 Motorized Focus7 Objective Nosepiece8 Leica SmartTouch 9 UV protection screen 1 2 3 4 5 7 9 8 6

Leica M-series User Manual 66 The Correct Interpupillary Distance The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. If you are still a novice microscope user, you may need a short time to become accustomed to this. Not to worry—after a ...

Leica M-series User Manual 67 If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups clockwise towards the rear, as otherwise the viewing distance is too great. 2. If an eyepiece is equipped with the inte- grated dioptric correction, turn the value to the "0" mark. By the wa...

Leica M-series User Manual 69 Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? The resistance can be adjusted individually depend-ing on the equipment weight and personal preferences as follows...

Leica M-series User Manual 71 The zoom button can optionally be operated either with or without ratchet steps. Continu-ous zoom is possible when the ratchet steps are disabled, which many users find convenient. On the other hand, when the ratchet steps are enabled, photographs, measurement results e...

Leica M-series User Manual 72 Parfocality: More Comfort and Convenience for Your Work All Leica stereomicroscopes are parfocally matched, meaning that you can view a focused specimen from the lowest to the highest magnification without having to refocus. The focus needs only be readjusted if you wan...

Leica M-series User Manual 73 The iris diaphragm in the optics carrier of your M series microscope fulfills the same purpose as the iris diaphragm in a camera: it regulates the available light, which changes the depth of field. The "depth of field" (or "focus depth") is the area of a...

Leica M-series User Manual 75 Magnification Factors of the Eyepieces An eyepiece not only makes it possible to look passively into the micro-scope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x. The following eyepieces are available for ...

Leica M-series User Manual 76 Health Notes Potential sources of infection •  Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered s...

Leica M-series User Manual 77 All Leica eyepieces are also available with built-in dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises ±5 diopter settings. Using the Dioptric Correction 1. Set the dioptric correction of...

Leica M-series User Manual 78 Adjusting 1. Set the dioptric correction for both eyepieces to "0". 2. Select the lowest magnification and focus on a flat specimen. 3. Select the highest magnification and read- just the sharpness. 4. Select the lowest magnification again, but do not look into ...

Leica M-series User Manual 79 Graticules 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the grati-cule fits tightly. 3. Screw the insert and graticule firmly into place and replace the eyepiece in the tube. 4. You can now align the graticule by rotating the eyepiece in...

Leica M-series User Manual 82 Photo Tubes and C-mounts Application All Leica DFC cameras are equipped with a standardized C-mount interface. In turn, the C-mount adapter for the respective trinocular tube is connected to this interface. This adapter creates a solid mechanical connection between the ...

Leica M-series User Manual 86 Use The objective nosepiece enables you to switch between two objectives using just one hand movement, for example between a 1× and a 2× planapochromat. Parfocal work With the new M series objectives, the parfo-cality is maintained even when objectives are changed, mean...

Leica M-series User Manual 88 The Different Types of Objectives To meet the various requirements regarding imaging properties, there is a choice of high-quality interchangeable planachromatic and planapochromatic objectives and also lower-priced interchangeable achromatic objectives. Achromatic obje...

Leica M-series User Manual 90 Leica TL ST Transmitted-light Base: Controls 1 Adapter plate for easy assembly of focusing drives 2 Removable glass plate3 Controller for light intensity4 Adjustment for deflection mirror Rear side of the transmitted-light base TL ST1 Screws for changing the halogen lam...

Leica M-series User Manual 91 Leica TL ST Transmitted-light Base: Operation Light intensity control The left control adjusts the intensity of the 12 V/20 W halogen illumination. 1. Switch on the illumination of the base at the power switch. 2. Focus on the specimen. 3. Set the illumination to the de...

Leica M-series User Manual 92 Changing the halogen lamp   Before you change the lamp, it is abso-lutely necessary to unplug the power plug from the base to prevent the risk of electric shock!   The halogen lamp becomes very hot during operation. Therefore, to avoid being burned, let the base cool of...

Leica M-series User Manual 93 Leica TL BFDF Transmitted-light Base: Controls 1 Adapter plate for easy assembly of focusing drives 2 Standard stage 10 447 2693 Button to toggle between bright field and dark field 1 2 3 Extension plate of the Transmitted-light base TL BFDF Adapter at the focusing driv...

Leica M-series User Manual 94 Light intensity control •  Observe the user manual—in particu-lar, all safety regulations—from the manufacturers of the light guide and cold light source. Switch on the cold light source according to ρ the manufacturer's user manual and adjust the brightness. Transmitte...

Leica M-series User Manual 96 Leica TL RCI™: The Deflection Mirror Inverted world? Depending on the properties of the specimen (refractive index of the environment) and the perception of the viewer, it is possible that the switches described below for positive and inverted relief contrast are to be ...

Leica M-series User Manual 97 Leica TL RCI™: Color Intensity and Temperature 1 Potentiometer for controlling the CCIC™ (Constant Color Intensity Control) illumina-tion intensity 2 Potentiometer for controlling the color temperature The transmitted-light base TL RCI™ has two electronic potentiometers...

Leica M-series User Manual 99 Leica TL RCI™: Methods in Transmitted Light Vertical bright-field illumination Suitable for stained amplitude specimens with sufficient contrast. The light beams are deflected vertically through the specimen. This results in an accurate bright field with maximum brightn...

Leica M-series User Manual 100 Leica TL RCI™: Relief Images Starting position 1. Push the deflection mirror all the way back towards the column. 2. Turn the deflection mirror into the notch position at an angle of 45°. Positive relief contrast Suitable for semitransparent and transparent specimens. ...

Leica M-series User Manual 102 Filters for Leica TL RC™ and TL RCI™ The transmitted-light bases TL RC™ and TL RCI™ can be equipped with up to three filters—available as accessories—simultaneously. By customer request, the filters are also available as one-off items. 1. Switch off the light source or...

Leica M-series User Manual 109 The Leica EL6000 compact light source is intended for biological routine and research work, including the examination of specimens from the human body for the purpose of gain-ing information. •  The Leica EL6000 compact light source is intended exclusively for operatin...

Leica M-series User Manual 112 Leica EL 6000 – Troubleshooting Fault description Possible faults Remedy Power indicator is not illuminated after being switched on, instrument shows no function No power supply voltage present; connecting cable not plugged in correctly/defective Check power supply vol...

Leica M-series User Manual 113 Cleaning and Maintenance •  Before carrying out cleaning tasks, switch off the Leica EL6000 compact light source. Use only a dry, lint-free cloth for cleaning. When cleaning, ensure that no dust or other foreign bodies enter the device through the ventilation slots. In...

Leica M-series User Manual 115 Filter Changer The patented FLUOIII® filter system consists of a filter changer for barrier and excitation filters, a UV shutter that can be activated/deactivated, and a filter insert for a filter holder with your choice of filter. The filter changer can hold a total o...

Leica M-series User Manual 116 FIM – Fluorescence Intensity Manager FIM consists of a diaphragm disk with pinhole diaphragms of varying transmission capaci-ties. The intensity of the excitation light can be reduced in five fixed steps. The light can be changed from 100% to 55%, 30% and 17% down to 1...

Leica M-series User Manual 117 About Fluorescence Microscopy Functional principle Fluorescent substances light up when irradi-ated with short-wave excitation light, and fluo-rescence microscopy takes advantage of this property. Specimens without self-fluorescence are stained with a fluorescent subst...

Leica M-series User Manual 118 Rapid Filter Changers and Filter Types Filter sets Excitation filter Barrier filter Designation GFP 425/60 nm (395–455 nm) 480 LP GFP1 GFP Plus 480/40 nm (460–500 nm) 510 LP GFP2 GFP plants 470/40 nm (450–490 nm) 525/50 nm (500–550 nm) GFP3 UV 360/40 nm (340–380 nm) 42...

Leica M-series User Manual 120 Equipping the Filter Changer •  When inserting the filter sets, avoid touching the filters in order to avoid fingerprints. Clean dirty filters immediately using a soft, lint-free cloth and pure alcohol. Removing the simple filter holder The Leica M165 FC and the Leica ...

Leica M-series User Manual 122 Double Iris Aperture The built-in double-iris diaphragm regulates the depth of field. Note that: The greater the depth of field, the darker ρ the image becomes. In the same way, the exposure time ρ becomes longer for photographs. The resolution decreases. ρ Adjusting t...

Leica M-series User Manual 123 Commissioning the Fluorescence System After switching on the high-pressure mercury burner, you must adjust the arc lamp imme-diately (see User Manual M2-216-1). Do not switch on the supply unit until you feel confi-dent using the Leica stereomicroscope. •  Follow all s...

Leica M-series User Manual 125 With its non-slip controllers and knobs, the Leica SmartMove control unit offers the right feel for handling motorized Leica instruments. Controls The functions of the individual controls can be configured both directly on the Leica Smart-Move and using the Leica Appli...

Leica M-series User Manual 126 With the touchscreen of the Leica SmartTouch™, you can control your experiment procedures with a few hand movements and never lose sight of all your important optical parameters. The most important control functions on the control unit can be adjusted to your specific ...

Leica M-series User Manual 127 Leica PSC Controller Use The Leica PSC controller gives you precision control of the Leica IsoPro™ motorized mechan-ical stage. Connection Connect the Leica PSC controller and the Leica IsoPro™ motorized mechanical stage to the DCI box. Passing over the specimen quickl...

Leica M-series User Manual 128 Dimensional Drawings

Leica M-series User Manual 137 MATS TL 190 140 220 4 11 134 98 170 10 447 164 10 447 275

Leica M-series User Manual 143 Leica EL6000 – Specifications Dimensions (W×H×D) Width×height×depth 130 mm × 210 mm × 290 mm Environmental conditions Permitted ambient temperature -20 to 85 °C Permitted relative humidity 10% to 90%, non-condensing Operation Permitted ambient temperature 0 to 40 °C Pe...

Leica M-series User Manual 145 Leica TL ST Transmitted-light Base Light source Halogen lamp 12V/20W Quick illuminant change Yes Illuminated area 50 mm Power supply unit Input voltage 100–240 V~, frequency 50–60 Hz energy consumption 30 W max., ambient temperature 10 – 40 °C Connections Power plug, p...

Leica M-series User Manual 146 Leica TL BFDF Transmitted-light Base Light source External via cold light source Illuminated area 40 mm Connections Connection for cold light guide, active f=10mm, end tube f=13mm Weight 5.8 kg Illumination types Bright field Yes Dark field Yes Oblique light No Relief ...

Leica M-series User Manual 147 Leica TL RC™ / TL RCI™ Leica TL RC™ Leica TL RCI™ Light source External via cold light source Halogen lamp 12V/20W Quick illuminant change – Yes Illuminated area 35 mm 35 mm Power supply unit – 100–240 V~, frequency 50–60 Hz energy consumption 30 W max., ambient temper...

Leica M-series User Manual 148 Leica IsoPro™ Motorized XY Stage Compatibility Leica incident light base and TL bases (TL BFDF, TL RC™, TL RCI™) Stage size (L × W × H) 335.5 mm × 370 mm × 41.5 mm Travel 152 mm × 102 mm (6" × 4") Speed X,Y 30 mm/s Motor resolution X,Y 0.25 µm Repeatability X, ...

Leica M-series User Manual 150 Calculating the Total Magnification and Field of View Diameter Parameter MO Magnification of the objective ME Magnification of the eyepiece z Magnification of the changer position q Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° ErgoTube™ r Factor 1.25...