Page 2 - General Instructions
Leica M-series User Manual 2 General Instructions Safety concept Before using your microscope for the first time, please read the "Safety concept" brochure included with your instrument. It contains addi-tional information about handling and care. Use in clean rooms The Leica M series can be...
Page 3 - Important Safety Notes
Leica M-series User Manual 3 Important Safety Notes User Manual The individual modules of the Leica M stereo-microscopy series include an interactive CD-ROM with all relevant user manuals in 20 other languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are...
Page 4 - Symbols Used
Leica M-series User Manual 4 Symbols Used Warning of a danger • This symbol indicates especially impor-tant information that must be read and complied with. Failure to comply can cause the following: ★ Hazards to personnel ★ Functional disturbances or damaged instruments Warning of hazardous electr...
Page 5 - Safety Regulations
Leica M-series User Manual 5 Safety Regulations Description ★ The individual modules fulfill the highest requirements for observation and docu-mentation of Leica stereomicroscopes of the M series. Designated use ★ Refer to "Safety Concept" booklet Non-intended use ★ Refer to "Safety Conc...
Page 9 - Inhaltsverzeichnis; Table of Contents
Inhaltsverzeichnis General Instructions 2 Important Safety Notes 3 Symbols Used 4 Safety Regulations 5 Safety Regulations (cont'd.) 6 Safety Regulations (cont'd.) 7 Safety Regulations (cont'd.) 8 The Leica M series Congratulations! 12 The Modular Design: Everything is Relative 13 Maximum Compatibili...
Page 13 - The Modular Design: Everything is Relative
Leica M-series User Manual 13 specific solutions. If you have a special request that cannot be met with standard parts, contact your Leica consultant. We have a solution for every problem. The Modular Design: Everything is Relative The Leica M series provides maximum flexibil-ity in choosing equipme...
Page 14 - Maximum Compatibility
Leica M-series User Manual 14 Leica engineers were careful to ensure that the new Leica M series—like its predecessors—remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused. Objectives All objectives of this new generation have the same reference f...
Page 15 - On We Go
Leica M-series User Manual 15 On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the instal-lation instructions and go directly to the Quick Start Guide on Page 29. If, on the other hand, you are assembling the micro...
Page 16 - Assembly
Page 17 - Base and Focusing Column
Leica M-series User Manual 17 The first step is to connect the focusing column of the M series to the corresponding base. Tools used ★ Hex socket screwdriver, 3 mm Assembling the column adapter 1. Remove the three screws provided from the column. 2. Install the base on the column using the three scr...
Page 18 - Microscope Carrier
Leica M-series User Manual 18 Tools ★ Allen screwdriver provided Assembly The microscope carrier is fastened to the column using the screw provided. Alternative installation options Depending on the desired working distance, the microscope carrier can be installed in the stan-dard way or upside down...
Page 19 - Optics Carrier
Leica M-series User Manual 19 Tools used ★ None Assembling the optics carrier 1. Unscrew the clamping screw on the holder for the optics carrier. 2. Place the optics carrier into the holder and align it in such a way that the notch in the optics carrier and the bottom clamping screw are on top of ea...
Page 20 - Tube
Leica M-series User Manual 20 Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner. Tools used ★ No tools required. Preparation 1. Unscrew the positioning screw and remove the protective cover. Assembling the tube 2. Push the tube (for ...
Page 21 - Eyepieces
Leica M-series User Manual 21 Eyepieces Tools used ★ No tools required. Magnification range You can extend the overall magnification range using available 10×, 16×, 25× and 40× wide-field eyepieces for persons wearing glasses. Preparation 1. If you want to use an optional graticule, insert it now (P...
Page 22 - Objective
Leica M-series User Manual 22 Objective Tools used ★ No tools required. Preparation 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective • Hold the objective firmly during assem-bly and disassembly so that it does not fall onto the stage plate. This applies part...
Page 25 - Leica LED3000 NVITM (Near Vertical Illumination)
Leica M-series User Manual 25 Required tools ★ None The Leica LED 3000 NVI™ can be used with any objectives that have an outer diameter of 58 mm. The supported working distance is between 50 mm and 150 mm. Installing the Leica LED3000 NVI™ 1. Connect the Leica LED3000 NVI™ to the focusing column...
Page 27 - Quick Start Guide
Page 28 - The Reliable Route to Success
Leica M-series User Manual 28 The Reliable Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner, and naturally you want to get right to work. This manual will familiarize you with the finer details of your micro-scope. The following ...
Page 29 - Overview of an M Series Microscope
Leica M-series User Manual 29 Overview of an M Series Microscope 1 Eyepieces for spectacle wearers with dioptric correction and eyecups 2 Fastening screws for the eyepieces3 Ergo tube4 Optics carrier5 Magnification changer (zoom)6 Interchangeable objective7 Incident-light base8 Column 9 Cable duct w...
Page 30 - The Correct Interpupillary Distance
Leica M-series User Manual 30 The Correct Interpupillary Distance The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. If you are still a novice microscope user, you may need a short time to become accustomed to this. Not to worry—after a ...
Page 31 - Using the Eyepieces
Leica M-series User Manual 31 If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups counterclockwise towards the rear, as otherwise the viewing distance is too great. 2. If an eyepiece is equipped with the inte- grated dioptric correction, turn the value to the "0" mark. By...
Page 32 - Focusing
Leica M-series User Manual 32 Focusing Focusing raises or lowers the stereomicroscope using the focusing drive. The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective. ★ The focusing drive can be operated either left- or right-handed. • The coarse/fine ...
Page 33 - Adjusting the Resistance of the Focus Drive
Leica M-series User Manual 33 Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? The resistance can be adjusted individually depend-ing on the equipment weight and personal preferences as follows...
Page 35 - Ratchet Steps and Magnification Levels
Leica M-series User Manual 35 The zoom button can optionally be operated either with or without ratchet steps. Continu-ous zoom is possible when the ratchet steps are disabled, which many users find convenient. On the other hand, when the ratchet steps are enabled, photographs, measurement results e...
Page 37 - Magnification Factor of the Eyepieces
Leica M-series User Manual 37 Magnification Factor of the Eyepieces An eyepiece not only makes it possible to look passively into the micro-scope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x. The following eyepieces are available for t...
Page 38 - Health Notes
Leica M-series User Manual 38 Health Notes Potential sources of infection • Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered s...
Page 39 - Dioptric Correction
Leica M-series User Manual 39 All Leica eyepieces are also available with built-in dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises ±5 diopter settings. Using the Dioptric Correction 1. Set the dioptric correction of...
Page 40 - Dioptric Correction and Parfocality
Leica M-series User Manual 40 Adjusting 1. Set the dioptric correction for both eyepieces to "0". 2. Select the lowest magnification and focus on a flat specimen. 3. Select the highest magnification and read- just the sharpness. 4. Select the lowest magnification again, but do not look into ...
Page 41 - Graticules
Leica M-series User Manual 41 Graticules 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the grati-cule fits tightly. 3. Screw the insert and graticule firmly into place and replace the eyepiece in the tube. 4. You can now align the graticule by rotating the eyepiece in...
Page 45 - Photo Tubes and C-mounts
Leica M-series User Manual 45 Photo Tubes and C-mounts Application All Leica DFC cameras are equipped with a standardized C-mount interface. In turn, the C-mount adapter for the respective trinocular tube is connected to this interface. This adapter creates a solid mechanical connection between the ...
Page 48 - The Different Types of Objectives
Leica M-series User Manual 48 The Different Types of Objectives To meet the various requirements regarding imaging properties, there is a choice of high-quality interchangeable planachromatic and planapochromatic objectives and also lower-priced interchangeable achromatic objectives. ★ Achromatic ob...
Page 49 - System Illumination
Page 52 - Dimensional Drawings
Page 62 - Appendix
Page 63 - Calculating the Total Magnification and Field of View Diameter
Leica M-series User Manual 63 Calculating the Total Magnification and Field of View Diameter Parameter MO Magnification of objective ME Magnification of eyepiece z Magnification changer position q Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° ErgoTube™ r Factor 1.25x if the planach...