Page 2 - Copyright
Leica M series User Manual 2 All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leic...
Page 3 - Function of the Microscopes; IVD
Leica M series User Manual 3 Function of the Microscopes The Leica M125 B and Leica M165 C B micro-scopes, for which this User Manual has been written, are designed for routine examinations of cell and tissue cultures, liquids, and sedi-ments. This includes examining specimens taken from the human...
Page 4 - General Notes
Leica M series User Manual 4 General Notes Use in clean rooms The Leica M series can be used in clean rooms without any problems. Cleaning ★ Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning. ★ Never use chemicals to clean colored surfaces or accessories with rubberized...
Page 5 - Important Safety Notes
Leica M series User Manual 5 Important Safety Notes User Manual This User Manual describes the special func-tions of the individual modules of the Leica M stereomicroscopy series and contains impor-tant instructions for their operational safety, maintenance, and accessories. You can combine individu...
Page 6 - Symbols Used
Leica M series User Manual 6 Symbols Used Warning! Safety hazard! • This symbol indicates especially impor-tant information that is mandatory to read and observe. Failure to comply can cause the following: ★ Hazards to personnel ★ Functional disturbances or damaged instruments Warning of hazardous ...
Page 7 - Safety Instructions
Leica M series User Manual 7 Safety Instructions Description ★ The individual modules fulfill the highest requirements for observation and docu-mentation of Leica stereomicroscopes of the M series. Intended use of instrument ★ Leica Microsystems microscopes are opti-cal instruments for improving the...
Page 11 - Contents
Leica M series User Manual 11 Contents Quick Start Guide The Fastest Route to Success 29 Overview of an M Series Microscope 30 The correct interpupillary distance 31 Using the Eyepieces 32 Focusing 33Adjusting the Resistance of the Focus Drive 34 Changing the Magnification (Zoom) 35 Click Stops and ...
Page 13 - The Leica M Series
Page 15 - A Step Towards Infinity
Leica M series User Manual 15 A Step Towards Infinity Ever since their introduction by Horatio S. Gree-nough, stereomicroscopes have worked accord-ing to the optical principles based primarily on Ernst Abbe's research. For over a century, ingenious optics designers and engineers have worked to push ...
Page 17 - Maximum Compatibility
Leica M series User Manual 17 Leica engineers were careful to ensure that the new Leica M series—like its predecessors—remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused. Objectives All new objectives of the Leica M series are parfocal. If you p...
Page 18 - On We Go
Leica M series User Manual 18 On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on page 28. If, on the other hand, you are assembling the micros...
Page 19 - Assembly
Page 20 - Base and Focusing Column
Leica M series User Manual 20 The first step is to connect the focusing column of the M series to the corresponding base. Tools used ★ Allen key, 3 mm Assembling the column adapter 1. Securely install the column adapter on the column using the four included screws. Assembling the focusing drive 2. S...
Page 21 - Optics carrier
Leica M series User Manual 21 Tools used ★ Allen key, 4 mm Assembling the Optics Carrier 1. Place the optics carrier on the focus- ing column so that the screw fits into the thread provided and the lug fits into the groove. 2. Press the optics carrier backwards to the focusing column and screw it in...
Page 22 - Tube
Leica M series User Manual 22 Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner. Tools used ★ No tools required. Preparations 1. Unscrew the positioning screw and remove the protective cover. Assembling the tube 2. Push the tube (for...
Page 23 - Eyepieces
Leica M series User Manual 23 Eyepieces Tools used ★ No tools required. Magnification range You can extend the overall magnification range using available 10×, 16×, 25× and 40× wide-field eyepieces for persons wearing glasses. Preparation 1. If you want to use an optional graticule, insert it now (p...
Page 24 - Objective
Leica M series User Manual 24 Objective Tools used ★ No tools required. Preparation 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective • Hold the objective firmly during assem-bly and disassembly so that it does not fall onto the stage plate. This applies part...
Page 25 - TL5000 Ergo transmitted light base
Leica M series User Manual 25 TL5000 Ergo transmitted light base Unpacking the base The base is delivered with the adapter plate installed. Make sure the instruments are unpacked on a flat, sufficiently dimensioned, and non-slip surface. Refer also to the separately provided User Manual for the TL...
Page 29 - The Fastest Route to Success
Leica M series User Manual 29 The Fastest Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner, and naturally you want to get right to work. Therefore, your next step should be to study the Quick Start Guide, which outlines the most ...
Page 30 - Overview of an M Series Microscope
Leica M series User Manual 30 Overview of an M Series Microscope 1 Switch click stops on/off2 Magnification changer (zoom)3 Coarse/fine focusing4 Transmitted light base5 Interchangeable objective6 Built-in iris diaphragm 7 Fastening screw for the binocular tube (or accessories)8 Adjustable eyepiece ...
Page 31 - The correct interpupillary distance
Leica M series User Manual 31 The correct interpupillary distance The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. If you are still a novice microscope user, you may need a short time to become accustomed to this. Not to worry—after a ...
Page 32 - Using the Eyepieces
Leica M series User Manual 32 If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups counterclockwise towards the rear, as otherwise the viewing distance is too great. 2. If an eyepiece is equipped with the inte- grated dioptric correction, turn the value to the "0" mark. By...
Page 34 - Adjusting the Resistance of the Focus Drive
Leica M series User Manual 34 Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too loose or too tight? Does the equipment tend to slide downwards? The resistance can be adjusted individually depending on the equipment weight and personal preferences as follo...
Page 36 - Click Stops and Magnifications
Leica M series User Manual 36 The zoom button can optionally be operated either with or without ratchet steps. Continu-ous zoom is possible when the click stops are disabled, which many users find convenient. On the other hand, when the click stops are enabled, photographs, measurement results etc. ...
Page 37 - Parfocality: More Comfort and Convenience for Your Work
Leica M series User Manual 37 Parfocality: More Comfort and Convenience for Your Work All Leica stereomicroscopes are parfocally matched, meaning that you can view a focused specimen from the lowest to the highest magnification without having to refocus. The focus needs to be readjusted only if you ...
Page 38 - Iris Diaphragm
Leica M series User Manual 38 The iris diaphragm in the optics carrier of your M series microscope has the same purpose as those in a camera shutter: it regulates the avail-able light, which changes the depth of field. The "depth of field" (or "depth of focus") is the area of a speci...
Page 40 - Magnification Factor of the Eyepieces
Leica M series User Manual 40 Magnification Factor of the Eyepieces An eyepiece not only makes it possible to look passively into the micro-scope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x. The following eyepieces are available for t...
Page 41 - Health Notes
Leica M series User Manual 41 Health Notes Potential sources of infection • Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered s...
Page 43 - Dioptric Correction and Parfocality
Leica M series User Manual 43 Adjusting 1. Set the dioptric correction for both eyepieces to "0". 2. Select the lowest magnification and focus on a flat specimen. 3. Select the highest magnification and read- just the sharpness. 4. Select the lowest magnification again, but do not look into ...
Page 44 - Graticules
Leica M series User Manual 44 Graticules 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the grati-cule fits tightly. 3. Screw the insert and graticule firmly into place and replace the eyepiece in the tube. 4. You can now align the graticule by rotating the eyepiece in...
Page 49 - Leica TL5000 Ergo Transmitted Light Base: Operation
Leica M series User Manual 49 2. Press the on/off switch on the right side once to turn on the illumination. Switching the transmitted light base on and off 1. Switch on the transmitted light base with the power switch on the rear side. Leica TL5000 Ergo Transmitted Light Base: Operation The trans...
Page 50 - Leica TL5000 Ergo Transmitted Light Base: Operation (Continued)
Leica M series User Manual 50 4 Press the on/off switch on the right side once again to turn off the illumination on the base. Leica TL5000 Ergo Transmitted Light Base: Operation (Continued) 3. Look through the eyepiece and adjust the light intensity using the control on the right side. The intensit...