Leica DM4000B- User Manual

7 1. Important Notes about this Manual (1.2) → p. 20 ! * Numbers in parentheses, such as "(1.2)", corre-spond to illustrations (in the example, Figure 1,Item 2). Numbers with pointer arrows (for example → p.20), point to a certain page of this manual. Special safety instructions are indicate...

10 3. Overview of the Instrument Specification Imaging Procedure Transmitted Light Axis Incident Light Axis Z Pinion Objective nosepiece X/Y Stage Tube 3. Overview of the Instrument Leica DM4000 B / DM5000 B • transmitted light: BF, DF, PH, Pol (DM5000 B also ICT) • incident light: fluorescence • in...

14 4. Unpacking the Microscope The device is delivered in two boxes. The stand box contains the following compo- nents: • Stand with integrated incident light axis and objective nosepiece • Specimen stage with stage bracket • Power cable and PC connecting cable • CD with Leica software package • Ins...

16 5. Assembly 5. Assembling the Microscope The microscope components are logically as-sembled in this order: • Stage• Condenser• Tube• Eyepieces• Objectives• Light sources• Filter cubes/reflectors* Only a few commonly used screwdrivers andkeys are necessary for assembly, which are in-cluded in the ...

19 5. Assembly 5.3 Tube and Eyepieces The tube is mounted to the stand either directly orwith the use of intermediate modules. It is fastenedin place with the side clamping screw (9.1). • Loosen the clamping screw (9.1). • Insert the tube in the circular receptacle (dovetail ring). • Retighten the c...

20 5. Assembly 5.5 Light Sources for the Transmitted Light Axis Caution: Be sure that the lamp housing is discon-nected from the power supply. Unplug thepower plug and the power supply during as-sembly. 107/2 Lamp Housing This lamp housing is used with a 12V 100W halo-gen lamp, which is already moun...

21 5. Assembly 5.6 Light Sources for the Incident Light Axis Caution: During assembly, always unplug the powersupply unit of the 106 z lamp housing from itssocket. Never touch the glass parts of the burnerwith bare hands. Never look directly into the beam path (blind-ing hazard). During assembly wor...

23 5. Assembly Inserting the gas discharge lamps (Hg and Xe)into the 106z lamp housing Hg and Xe lamps are powered by the separateebq 100 supply unit.Read the separate instruction manual providedwith this supply unit. The following gas discharge lamps may be usedand require different lamp mounts (Fi...

28 5. Assembly • Remove the plug cap on the left side of the stand. • Insert the polarizer into the receptacle until it latches in place (Fig. 30). Motorized analyzer • Insert the analyzer cube as described in sec- tion 5.7 "Equipping the Incident Light filterturret" → p. 26, in the correspo...

29 5. Assembly Ergomodule For raising the eye level of the tube opening, theergomodule may be used.It is fastened in place with the side clampingscrew. Mirror Housing • Place the mirror housing directly onto the lamp housing receptacle on the back of thestand and attach it using the side clampingscr...

31 6. Startup 6. Startup 6.1 Functional Principle The microscope’s most important functions may be easily accessed using function keys. • The microscope may be switched between various contrast processes by pressing a single button.• The microscope recognizes the objective chosen and the respective ...

34 6. Startup Fig. 37 Display after initialization • Switch-on the microscope at the power switch (34.1,36.1). All motorized microscopecomponents first undergo an initializationphase. After initialization is complete, the display on thestand shows the current microscope setting (Fig.37). The microsc...

38 6. Startup • Open the field diaphragm just enough for it to disappear from the field of view (41d). Caution: Do not adjust the aperture diaphragm. The aper- ture diaphragm is already set optimally for eachobjective. 6.6. Checking Phase Contrast Rings A B C D Fig. 41 Köhler Illumination a Field di...

39 6. Startup If your microscope is equipped for the use ofphase contrast, the light rings that fit the objec-tives are built into the condenser.The light rings are already leveled in the factory.However, the leveling should be rechecked. Note: Every objective is assigned its own light ring inthe co...

40 6. Startup • Insert the centering key through the corre- sponding openings (44.1) in the condenserholder. • Turn the centering screws until the dark ring (phase ring in the objective) is congruent withthe slightly narrower bright ring (light ring incondenser) (43 c). • Repeat the process for all ...

42 6. Startup • In the adjustment window, you see the direct filament image and the mirror image, which inmost cases are shifted together. • Focus the direct filament image with the col- lector (46.6). • Use the adjusting buttons on the rear side of the lamp housing (46.2, 46.4) to pivot the lampfil...

47 7. Operation Rotating the Stage The swiveling range of the rotating stages is0°- 110°. • In order to revolve the stage, loosen the fas- tening screw (59.1). • Bring the table into the desired position. • Retighten the fastening screw. Fig. 59 Revolving object stage 1 Clamping screw 2 Fine focusin...

48 7. Operation 7.4 Tubes Note: Close any unused tube openings, as otherwisestray light can interfere with observation. Note: Make sure that the connector cable is plugged inon the MBDT25+ motorized tube (60.1). Adjusting the Viewing Distance • Adjust the viewing distance of the eye- pieces so that ...

50 7. Operation 7.6 Objectives The objective must be moved manually into thelight path. Be sure that the nosepiece turretlocks into place. The objective’s position in the turret is factory-set and must be adhered to while screwing inthe objectives (see Objective Assembly → p. 19) When you rotate the...

51 7. Operation 7.7 Magnification Changer Optionally, a coded magnification changer canbe used, which is manually operated.On the knurled ring, the following magnificationfactors can be set: B Stand M Stand 1x 1x 1.25x 1.5x 1.6x 2x The selected factor is indicated in the displayand included in the t...

64 11. Care of the Microscope Caution! Unplug the power supply before performingcleaning and maintenance work!Protect electrical components from moisture! Microscopes in warm and warm-damp climaticzones require special care in order to preventfungus contamination.The microscope should be cleaned aft...

65 11. Care of the Microscope Cleaning Glass Surfaces Remove dust on glass surfaces with a fine, dryand fat-free hair brush, by blowing with a blowbag or vacuum suction. Carefully remove stubborn dirt on glass surfaceswith a clean cloth moistened with distilledwater. If the dirt still can not be rem...

66 12. Wear and Spare Parts Order No.Material No. Name Used for Replacement Lamp500 974 Halogen lamp 12 V 100 W 107/2 lamp housing 500 137 High-pressure mercury burner 50 W 106 z lamp housing 500 138 High-pressure mercury burner 100 W 106 z lamp housing 500 321 High-pressure mercury burner 100 W 106...

67 13. Abbreviations and Pictograms 13. Abbreviations and Pictograms Contrast method Magnification Light intensity/diaphragms Beam splitting Transmitted light shutter open Transmitted light shutter closed Incident light shutter open Incident light shutter closed ↓ ↓ ↑ ↑ + AET Advanced Ergo Tube AP A...

69 15. EU Declaration of Conformity 15. EU Declaration of Conformity