Leica RM2245- User Manual

7 Leica RM2245 2. Safety 2.1 Safety notes This instruction manual contains importantinstructions and information regarding theoperational safety and maintenance of theinstrument.The instruction manual is an important part ofthe product, which must be read carefully priorto startup and use and must a...

8 Instruction manual V 1.4 – 02/2007 2. Safety Warnings – Safety instructions / warning labels attached to the instrument • Safety instruction labels on the instrument marked with a warning triangle indicate that thecorrect operating instructions (as described in this manual) must be followed when o...

9 Leica RM 2245 Warnings – Working at the instrument • Take care when handling microtome knives and disposable blades. The cutting edge isextremely sharp and can cause severe injury! • Always remove the knife / blade before detaching the knife holder from the instrument. Alwaysput the knives back in...

10 Instruction manual V 1.4 – 02/2007 2.3 Integrated safety devices 2. Safety Locking the handwheel There are two ways of locking the handwheel ( 12 ): Using the lever ( 3 ) on the right side of the micro- tome base plate, the handwheel can be brakedin almost any position. • To brake, rotate the lev...

11 Leica RM2245 2. Safety Knife guard on the knife holder Each knife holder is equipped with a tightlymounted knife guard ( 8 , 9 ). This makes it possi- ble to cover completely the cutting edge inevery knife or blade position. Knife holder N/NZ The knife guard ( 8 ) of the knife holder N/NZ can be ...

12 Instruction manual V 1.4 – 02/2007 3.1 Overview – instrument components 3. Instrument components and specifications Leica RM2245 Knife guard on the knife holder Smooth-turning handwheel Lever of handwheel brake Handwheel locking mechanism Separate control panel Directional specimen holder fixture...

13 Leica RM2245 3. Instrument components and specifications Fig. 7 Vents Voltage selectorswitch with fuses Main switch Powerconnection Connecting cable for control panel Rear side of instrument Magnet for holdingthe wrench size 4 3.2 Instrument specifications The Leica RM2245 is a semi-motorized rot...

15 Leica RM 2245 Microtome Sectioning thickness setting: Section thickness setting range: 0.50 - 100 μm Setting values: from 0.50 μm - 5.0 μm in 0.5 μm increments from 5.0 - 20.0 μm in 1.0 μm increments from 20.0 - 60.0 μm in 5.0 μm increments from 60.0 - 100.0 μm in 10.0 μm increments Trimming sect...

16 Instruction manual V 1.4 – 02/2007 4.1 Standard delivery The Leica RM2245 standard delivery includes: 1 Leica RM2245 basic instrument, without orientation ................. 0501 381781 handwheel, complete ...................................................................... 0501 381811 external ...

17 Leica RM2245 Setting up the instrument Do not transport the instrument by holding it on the handwheelshaft, the object head or the cover! • By holding the instrument ( 5 ) at the front by the baseplate, and at the rear by the recessed grip (Fig. 8), lift it out of the molded cushion ( 6 ) of the ...

18 Instruction manual V 1.4 – 02/2007 4. Startup The feather key ( 4 ) is loosely placed in the handwheel shaft ( 1 ) and fixed in place with a tie-rap during transport. • Remove the cable tie.Caution!Make sure not to lose the feather key! • Place the handwheel ( 2 ) on the handwheel shaft ( 1 ) as ...

20 Instruction manual V 1.4 – 02/2007 4. Startup 4.5 Switching on the instrument Exposure to extreme temperature changes and high air humidity may cause condensation to forminside the instrument.After transporting, please wait at least 2 hours to allow the instrument to adopt the ambienttemperature ...

21 Leica RM 2245 5. Operation 5.1 Operating elements and their functions The operating functions of the microtome are divided between a control panel and a display uniton the microtome.A control panel on the instrument displays the current operating mode as well as various settings.All operating fun...

24 Instruction manual V 1.4 – 02/2007 In sectioning mode the user can select between STEP function (step-by-step specimen feed) and continuous specimen feed. When continuous feed is selected, the coarse feed buttons have the same func-tions as in trimming mode. The STEP function is useful for carefu...

25 Leica RM2245 5. Operation • To change the display mode, push MENU MODE until the LED of the desired mode is illuminated. • Press CLEAR to reset section thickness sum or section number. • This will only reset the currently displayed value. Caution!When the instrument is switched off using the main...

27 Leica RM2245 Indication of remaining horizontal feed The visible and audible remaining feed indica-tion feature informs the user during trimmingand sectioning when a remaining feed of ap-proximately 1 mm is available before the frontlimit is reached. The yellow LED ( 41 ) in the COARSE FEED butto...

29 Leica RM2245 Fig. 18 Fig. 19 33 34 Important!Never turn thescrew morethan 1 / 2 turn at a time. 5. Operation Fine adjustment of the force balance If another accessory of a different weight ismounted on the object head ( 33 ), you must check whether it is necessary to readjust theforce balance. Ch...

30 Instruction manual V 1.4 – 02/2007 Setting up the knife holder base • Release the clamping lever ( 50 ) by rotating it counterclockwise. • Insert the knife holder base ( 51 ) using the notch ( 52 ) on the bottom into the T-piece ( 55 ) of the microtome base plate ( 53 ). • To secure the knife hol...

31 Leica RM2245 5. Operation There are two versions of the specimen holder,one with and one without specimen orientation,which are interchangeable.The object orientation allows for simple positioncorrection of the specimen surface when thespecimen is clamped into place. You can use the quick clampin...

32 Instruction manual V 1.4 – 02/2007 5. Operation • The index marks (0 °, 5 ° and 10 °) for adjust-ment of the clearance angle ( 59.1 ) are located on the right side of the knife holder ( 57 ). • There is also an index mark ( 59.2 ) on the right side of the knife holder basis ( 51 ) which serves as...

33 Leica RM 2245 5. Operation 5.5 Clamping the specimen Always clamp the specimen block BEFORE clamping the knife.Lock the handwheel and cover the knife edge with the knife guardprior to any manipulation of knife or specimen, prior to changingthe specimen block and during all work breaks! • Rotate t...

34 Instruction manual V 1.4 – 02/2007 5. Operation 5.7 Cutting into the specimen (trimming) • Use the TRIM/SECT key to select the trim mode. • Set the desired trim section thickness. • Release the handwheel lock and the brake. • In TRIM mode, use the coarse feed buttons to move the sample against th...

35 Leica RM 2245 5.9 Changing the specimen or interrupting sectioning Lock the handwheel and cover the knife edge with the knife guardprior to any manipulation of knife or object head, as well as priorto changing the specimen block and during all work breaks! • Raise the specimen to the upper end po...

36 Instruction manual V 1.4 – 02/2007 6.1 Assembly for fixture for specimen clamps Depending upon the purchase order, the basic instrument is delivered with the directional orrigid fixture for specimen clamps which must be assembled first. All specimen clampsavailable as accessories can be used in b...

37 Leica RM2245 6. Optional accessories 6.1.3 Fine-directional fixture for specimen clamps • Before the fine-directional fixture forspecimen clamps can be mounted, loosen4 screws ( 10 ) (Allen key size 3) and carefully remove the fixture for specimen clamps fromthe baseplate ( 9 ). Fig. 26 10 11 • U...

38 Instruction manual V 1.4 – 02/2007 6.1.4 Quick clamping system It is used as specimen holder for use with thefine-directional fixture for specimen clampswith zero point indicators or the directionalfixture for specimen clamps. • Screw the 4 screws ( 13 ) into bore A with an Allen key size 2.5 and...

39 Leica RM2245 6. Optional accessories 6.2.1 Standard specimen clamp The standard specimen clamps are available intwo sizes: 40 x 40 mm and 50 x 55 mm.They are designed for direct clamping ofrectangular blocks. In addition, they accommo-date the foil clamps. • Turn the knurled screw ( 66 ) counterc...

40 Instruction manual V 1.4 – 02/2007 6. Optional accessories Clamping of foil pieces • Move the movable jaw ( 74 ) to the right as required by turning the set screw with anAllen key size 4 ( 71 ). • Place the foil ( 72 ) between the movable jaw ( 74 ) and the fixed jaw ( 73 ). • To clamp the foil, ...

45 Leica RM2245 6. Optional accessories 6.3 Knife holder base and knife holder Repositioning the knife holder base The one-piece knife holder base (rigid) ( 51 ) can be moved forwards and backwards on themicrotome base plate. This vertical displacement allows bringing theknife holder into the optima...

46 Instruction manual V 1.4 – 02/2007 6. Optional accessories Prior to inserting the blade, both knifeholder and knife holder base must havebeen installed on the instrument! Inserting the blades, knife holder E and E-TC • Fold knife guard ( 9 ) downward. • To insert the blade, flap the right clampin...

47 Leica RM2245 6. Optional accessories Lateral displacement (only for knife holder E) The lateral movement feature of the knifeholder base enables the use of the entire lengthof the blade or knife, eliminating the need for re-adjusting the knife holder. The knife holder Econsists of a segment arch ...

48 Instruction manual V 1.4 – 02/2007 Knife holder E with water bath for narrow-band and broad-band blades 6. Optional accessories Fig. 42 Knife holder E with water bath is available forboth narrow-band and broad-band blades. The knife guard on knife holder E consists of ared foldaway handle ( 9 ). ...

49 Leica RM 2245 6.3.3 Knife holder N/NZ The knife holders N and NZ are appropriate for standard steel and tungsten carbide knives, profilec and d, up to 16 cm long. The integrated height adjustment feature allows you to also use knivesthat have been resharpened numerous times. Fig. 44 Enlarged deta...

50 Instruction manual V 1.4 – 02/2007 6. Optional accessories Inserting the knife • Rotate the knurled nuts ( 48 ) on the right and left of the knife holder forward in opposite directions, lowering the knife support bar to thelowest possible position, thus ensuring that the knife edge will not bedam...

51 Leica RM2245 6. Optional accessories The backlighting is inserted at the frontinto the one-piece knife holder base. • Remove the two screws ( 1 ) using a slotted screwdriver and then remove the coverplate ( 2 ). • Insert the backlighting ( 3 ) in the recess at the rear of the knife holder base. •...

53 Leica RM2245 6.8 Universal microscope carrier 6. Optional accessories 1 - Baseplate with drilling (a)2 - Vertical column with size 8 cap screw (b) and lock washer (c) 3 - Horizontal arm with cross-member (d) and support ring (e) 4 - Support plate, large (for RM2235, RM2245 and RM2255) 5 - Support...

55 Leica RM2245 6. Optional accessories 6.9 Magnifying lens Fig. 55 Fig. 57 Fig. 56 1 3 T h e m a g n i f y i n g l e n s p r o v i d e s a 2 xmagnification and can be used with allLeica 2200 series rotary microtomes. 2 4 Always protect the magnifier glass fromexposure to direct sunlight! Exposure t...

56 Instruction manual V 1.4 – 02/2007 2 4 5 6 6. Optional accessories 6.9 Cold light source 6.10 Fiber-optic light guide Fig. 62, Leica rotary microtome with installed optionalaccessories: magnifying lens and fiber-optic light guide Fig. 58 Fig. 60 Fig. 59 The cold light source may be operatedat the...

57 Leica RM2245 6.11 Ordering information Fixture for specimen clamps, rigid, silver ............................................................................. 0502 38160 Fixture for specimen clamps, directional, silver ................................................................. 0502 38949 ...

58 Instruction manual V 1.4 – 02/2007 6. Optional accessories EM specimen holder fixture, black ....................................................................................... 0502 29968 EM univ. specimen holder ...................................................................................

59 Leica RM 2245 7. Customized solutions Certain instrument malfunctions appear on the 3-digit LED displayor are indicated through illuminated LED’s on the control panel. 7.1.1 Error reports Indication E1 If this message follows the software version indication after switching onthe instrument, a los...

60 Instruction manual V 1.4 – 02/2007 7. Customized solutions • Feed motion no longer occurs. Problem Corrective action Possible cause 1. STEP mode is still active (LED in SECT button lights up). 1. The end of the residual path has been reached. 2. The specimen was already within the residual path w...

61 Leica RM 22 45 7. Customized solutions 1. Thick/thin sections The sections alternate betweenbeing thick and thin. In extremecases, there are no sectionswhatsoever. • Check the clamping positions and repeat the clampingprocess • Laterally displace the knife holder or insert a new blade. • Insert a...

62 Instruction manual V 1.4 – 02/2007 Cleaning the knife Always wipe the knife from the back of the knife to the cutting edge. NEVER wipe in theopposite direction - risk of injury! Clean using an alcohol-based solution or acetone. Before each cleaning carry out the following preparatory steps: • Rai...

63 Leica RM2245 8. Cleaning and maintenance Knife holder E Fig. 61 10 11 9 Fig. 62 86 83 83 86 87 11 10 Take the knife holder apart for cleaning. To doso, proceed as follows: • Fold the knife guard ( 9 ) and ejector ( 84 ) downward. • Rotate the clamping lever ( 11 ) of the lateral dis- placement fo...

64 Instruction manual V 1.4 – 02/2007 Universal cassette clamp • Detach cassette clamp ( 13 ) for a thorough cleaning, removing all paraffin residues. • For cleaning, do not use xylene. Use xylene substitutes or paraffinremovers such as "Para Gard". • The cassette clamp ( 13 ) can also be pl...

65 Leica RM2245 8.2.2 Maintenance instructions Only authorized and qualified service personnel may access the internal components ofthe instrument for service and repair! The instrument is basically maintenance-free. To ensure trouble-free operation of the instrument over a long period of time, the ...

66 Instruction manual V 1.4 – 02/2007 8. Cleaning and maintenance 97 88 73 11 10 90 Universal cassette clamp: • Shaft ( 90 ) and flat coil spring ( 91 ) of the clamping lever of the cassette clamp. Afterlubrication, move the lever back and forthseveral times. Knife holder E: • Clamping lever ( 11 ) ...

67 Leica RM 2245 9. Warranty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractualproduct delivered has been subjected to a comprehensive quality controlprocedure based on the Leica in-house testing standards, and that theproduct is faultless and complies with all tec...

. . . . . . . . . . . . . . . . . . . . . . . . . . . . Dag Graupner Managing Director EC Declaration of Conformity Leica Microsystems Nussloch GmbHPostfach 1120D-69222 Nussloch January 12, 2004 10. EC Declaration of Conformity We herewith declare, in exclusive responsibility, that the Leica RM2245 ...