Page 3 - Contents; fluorescence stereomicroscope; Leica stereo-fluorescence module
3 Contents Page Leica MZ10 F fluorescence stereomicroscope Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....
Page 5 - Light source: Safety regulations
5 Servicing and repairs – Repairs may only be carried out by Leica-trained service technicians or by technical personnel authorized to do so by the person in charge of the instrument – Only original Leica spare parts may be used– Unplug the power cable before opening the supply unit . Touching live ...
Page 6 - Applications
6 Applications Natural science Application Fluorescence filter set Anatomy • Monitoring of capillary flow • GFP 1, GFP 2 Biology • Gene expression in chicken embryos, fruit flies, • GFP 1, GFP 2 threadworms and zebra fish • Fish otoliths marked with alizarine red • Green • Genetic expression through...
Page 8 - Overview; Leica MZ10 F: Its components and controls
8 Overview Leica MZ10 F: Its components and controls 1 . Choice of stand and of focusing drive (manual or motor-driven) 2 . Microscope carrier for Leica MZ10 F3a . Leica MZ10 F optics carrier with third beam path3b . Integrated FLUOIII filter system 3c . Adapter for light source3d . Rapid filter cha...
Page 9 - Filter sets for Leica MZ10 F
9 Filter sets Designation Excitation filter Barrier filter GFP fluorescence GFP1 425/60 nm 480 nm GFP Plus fluorescence GFP2 480/40 nm 510 nm GFP plant fluorescence GFP3 470/40 nm 525/50 UV fluorescence UV 360/40 nm 420 nm Violet fluorescence V 425/40 nm 475 nm Blue fluorescence B 470/40 nm 515 nm G...
Page 12 - Lamp housing
12 Assembly Lamp housing The Leica 106Z lamp housing is equipped . For information about the supply units for the 106Z lamp housing, see page 14 . Model 105Z lamp housings, if available, can also be used . Observe the safety regulations on page 5 Notes and recommendations • Do not switch on and off ...
Page 16 - FLUOIII filter system
Dummy filter carrier In the dummy filter carrier there are two openings for the observation beam paths instead of the barrier filters . The third path has been closed . Use the dummy filter carrier: – if you are using less than four sets of filters– if you are working without the fluorescence illumi...
Page 17 - Use; MZ10 F: Bringing the specimen into focus and observing it
17 Use MZ10 F: Bringing the specimen into focus and observing it Observe the safety directions on page 4 and the recom- mendations on page 5 regarding the use of the mercu- ry-vapour lamp. Changing filters In the rapid filter changer, all four positions must be occupied, either with filter sets or w...
Page 18 - Outfit with stereo-fluorescence module
18 Overview Outfit with stereo-fluorescence module The components 1 Choice of stand and of focusing drive (manual or motor-driven) 2 Microscope carrier3 Leica MS5, MZ6, MZ7 .5 – 16 optics carrier4a Leica stereo-fluorescence module with choice of barrier fil- ter 4b Filter insert for excitation filte...
Page 19 - Filter sets for stereo-fluorescence module
19 Filter sets Excitation Dichromatic Barrier filters beam splitters filters GFP fluorescence 425/60nm 470nm GG475 GFP-Plus fluorescence 480/40nm 505nm LP 510nm LP GFP-Plant 470/40nm 495nm 525/50nm UV fluorescence 360/40nm 400nm GG420 Violet fluorescence 425/40nm 460nm GG475 Blue fluorescence 470/40...
Page 20 - Stereo-fluorescence module
20 Assembly Stereo-fluorescence module 1 Incident- or transmitted-light base 2a Manual focusing drive, or 2b Motorized focusing drive 3 Microscope carrier 4 Choice of optics carrier 5 Fluorescence module 6a 106Z lamp housing 6b Stray-light protection 7 Binocular tube 8 Clamp 9 Arm10 UV-light exclude...
Page 21 - Fluorescence module
21 Important: With the illuminator on, insert the filterhol- der with diaphragm into slide-in unit 3 to avoid being suddenly exposed to excessively bright light (page 22). ▶ Remove the binocular tube . ▶ Loosen the 3 socket-head screws . ▶ Remove the cover . ▶ Loosen the fastening screw and take out...
Page 24 - and representatives of Leica Microsystems; Microscopy Systems
Leica Microsystems – the brand for outstanding products Leica Microsystems – an international company with a strong network of customer services. Australia: Gladesville Tel . +61 2 9879 9700 Fax +61 2 9817 8358 Austria: Vienna Tel . +43 1 486 80 50 0 Fax +43 1 486 80 50 30 Canada: Richmond Hill/Onta...