Olympus FV300 - Manuals - manualsarea.com

Page 3 - OPE

2 OPE q e r t y u i o !0 !1 !2 w q Optical fiber for laser introduction w Beam collimator e Polarizer r Dichromatic mirror t Excitation dichromatic mirror y XY galvanometer mirror scanners u Pupil lens i Collector Lens o Pinhole turret !0 Emission beam splitter slider !1 Barrier filter slider !2 Pho...

Page 4 - Ultimate ease of operation and monitor display.; Software Graphical User Interface

Preset the conditions for imageacquisition and loading Storage of Acquisition Settings enablesimmediate, one-touch recall of all therelevant experimental settings andconditions. Adding new conditions oraltering existing ones is quick and easy. AOTF: flexible control of the laserintensity to meet the...

Page 6 - Time Course

5 X -t SlantLine-t FreeLine-t Y -t Y-Z-t X-Y-t Z o o m I n - t X-Z-t X-Y-t X-Y-Z-t Z o o m I n - Z - t Patch clamp BX61WI fixed stage upright microscope+translation stage Image acquisition ROI designation Intensity versus timemeasurement Calcium sparks in isolated cardiac myocyte Dr. Sandor GyorkeTe...

Page 7 - FRET

6 The 440nm diode laser can be addedfor CFP/YFP FRET imaging. A 440nm diode laser is optionally availablefor CFP/YFP imaging. The 440nm laser lineideally excites CFP, with minimaldisturbance to YFP, and is thereforesuitable for CFP/YFP FRET experiments.The high performance LSM objectives,PLAPO40XWLS...

Page 8 - PAPP for FRAP Application

7 PAPP for FRAP Application PAPP: Programmable Acquisition Protocol ProcessorEasy, reliable flow of experiments for fluorescence recovery after photobleaching. AOTF and PAPP function for effectiveFRAP (Fluorescence Recovery AfterPhotobleaching) Fluorescence recovery after photobleachingcan be analyz...

Page 9 - Multi-point time lapse system

8 Multi-point time lapse system High-magnification multi-point time lapse observation of living cells. Wide-ranging specimen observationsfor improved experiment throughput Use of a motorized XY stage allows theanalysis of time lapse changes in manypoints scattered over a wide area. Thesystem is ther...

Page 10 - D Imaging

9 Tile display Topographic projection Height of 3D structure indicated by color. X-Z Y-Z X-Y-Z X -Z Y -Z Using multiple 3D images to obtain accurate 3D structure analysis. 3D Imaging Easy Z axis operation and setting The upper and lower limit of Z scanning canbe specified interactively by actuallysc...

Page 11 - Analyzing the degree of intensity overlap between channels.

10 Link with patch clamping data • With PCs linked through a LAN, Physiolink can synchronize electrophysiological andconfocal image data simultaneously. • With the same time stamp recorded in the two PCs, it is possible to access andanalyze an image and its patch clampingdata corresponding to the Ph...

Page 12 - Applications Gallery; Morphology; Neuron

11 Lucifer Yellow: retina ganglion cellTexasRed: dopamine-operated amacrine cell Prof. Shigetada NakanishiDept. of Biological Sciences, Kyoto Univ. Faculty of Medicine Applications Gallery Morphology Structure of PtK2 cell Nucleus: DAPI (Blue)Actin: FITC (Green)Mitochondria: Mito Tracker (Red)Microt...

Page 13 - Plant

12 Fluorescent Proteins C elegans expressing beta-integrin fused to GFP Dr. Xioping Xhu and Dr. John PlenefischUniversity of Toledo, Dept. of Biology GFP-labeling of Drosophila adults Expression of DsRed in a zebrafish embryoExtended focus image of 5µmx30 slice Pr. Yasuhiro Kamei, Pr. Shunsuke YubaI...

Page 14 - Fluorescence Dyes and Filters

Fluorescence Dyes and Filters 300 400 500 600 700 BA530RIF BA550RIF BA650RIF BA430-460 BA465-495 BA480-495 BA470-520 BA510-540 BA520-550 BA505-550 BA505-525 BA480-510 BA535-565 BA540-590 BA560-600 BA585-615 BA510IF BA560IF BA565IF BA585IF BA590 BA610IF BA625IF BA660IF BA585-640 440 LD 405 LD 458 Mul...