Page 2 - FLUOVIEW–—From; Olympus is Open; FLUOVIEW—More Advanced than Ever; “imaging to analysis.”; Quality Performance with Innovative Design
1 FLUOVIEW–—From Olympus is Open FLUOVIEW—More Advanced than Ever The Olympus FLUOVIEW FV1000 confocal laser scanning microscope delivers efficient and reliable performance together with the high resolution required for multi-dimensional observation of cell and tissue morphology, and precise molecul...
Page 3 - Imaging to Analysis; ing up New Worlds; From Imaging to Analysis; Advanced Deeper Imaging with High Resolution
2 Imaging to Analysis ing up New Worlds From Imaging to Analysis FV1000 Advanced Deeper Imaging with High Resolution FV1000MPE
Page 4 - Evolving Systems Meet the Demands of Your Application.
Advanced FLUOVIEW Systems Enhance the Power ofYour Research Superb Optical Systems Set the Standard for Accuracy and Sensitivity. Two types of detectors deliver enhanced accuracy and sensitivity, and are paired with a new objective with low chromatic aberration, to deliver even better precision for ...
Page 6 - Two versions available.
Laser combiner/Fiber Diode Laser Greater stability, longer service life andlower operating cost are achieved usingdiode lasers. Laser Feedback Control Scanner unit is equipped with laser powermonitor for feedback control enhancingstable laser output. Laser Compatibility Diode laser : 405 nm, 440 nm,...
Page 7 - Optical System
Technology / Hardware IX81 PMT PMT PMT Galvanometerscanning mirrors Galvanometerscanning mirrors UIS2 objectives Specimen Pupil projection lens SIM Scanner * Microscope icity. Optical System Motorized Microscopes Compatible with Olympus IX81 invertedmicroscope, BX61WI focusing nosepieceand fixed-sta...
Page 8 - Spectral Based Detection; Flexibility and High Sensitivity; Filter Based Detection; Enhanced Sensitivity
Spectral Based Detection Flexibility and High Sensitivity Spectral detection using gratingsfor 2 nm wavelength resolutionand image acquisition matched tofluorescence wavelength peaks.User adjustable bandwidth ofemission spectrum for acquiringbright images with minimal cross-talk. Precise Spectral Im...
Page 10 - Low Chromatic Aberration Objective; Best Reliability for Colocalization Analysis; Improved Flatness and Resolution at 405 nm; Better flatness reduces the number of images for tiling.
Low Chromatic Aberration Objective Best Reliability for Colocalization Analysis A new high NA oil-immersion objective minimizes chromaticaberration in the 405–650 nm region for enhanced imagingperformance and image resolution at 405 nm. Delivers a highdegree of correction for both lateral and axial ...
Page 11 - Switchable between Confocal and TIRFM Imaging; FV1000MPE Multiphoton Excitation System; Brighter and Deeper Imaging with Finer Resolution; LSM
Technology / Hardware Switchable between Confocal and TIRFM Imaging Switchable between confocal and TIRFM imaging for localizationof proteins on the cytoplasmic membrane surface and acquisitionof sectioning images within cells. Software Control of TIRF Illumination Built-in laser provides TIRF illum...
Page 12 - User-Friendly Software to Support Your Research.; Time Controller; XY
User-Friendly Software to Support Your Research. Time Controller Precisely synchronizes differentexperimental protocols including FRAP,FLIP and FRET by acceptor photo-bleaching and time-lapse. Save and opensettings for later use. Wide Choice of Scanning Modes Several available scanning modesincludin...
Page 13 - Help Guide
Technology / Hardware Re-Use Function Open previously configured scanningconditions and apply them to new orsubsequent experiments. Help Guide Comprehensive help guide describes thefunctions and usage for each command,and overall sequence of operations. Multi Stimulation Software Configure multiple ...
Page 15 - High resolution images stitched to cover a large area.
Application 15,000 20,000 30,000 25,000 Time (ms) 35,000 40,000 200 400 600 800 1,000 1,200 1,400 1,600 1,800 2,000 2,200 2,400 Intensity CH1 CH2 CH1 CH2 Measurement Diffusion measurement and molecular interactionanalysis. Light Stimulation FRAP/FLIP/Photoactivation/Photoconversion/Uncaging. Multi-D...
Page 16 - Diffusion Measurement Package
This optional software module enables data acquisition and analysis to investigate the molecular interaction and concentrations by calculating the diffusion coefficients of molecules within the cell. Diverse analysis methods (RICS/ccRICS, point FCS/point FCCS and FRAP) cover a wide range of molecula...
Page 17 - Scan in X-Axis Direction; RICS Application and Principles; RICS Principle; RICS Analysis Method; At cytoplasmic membrane
Application/ Molecular Interaction Analysis 0 µs 0 ms 0 ms 1 ms 2 ms 3 ms 4 ms n ms 10 µs 20 µs 30 µs 40 µs 50 µs n µs Molecule size Small Large Spatial Correlation Algorithm When the spatial correlation algorithm is applied between pixels, a higher correlationis obtained as the speed of movement of...
Page 18 - Laser Light Stimulation; imaging of the effects of stimulation on the cell.; FLIP—Fluorescence Loss in Photobleaching
Laser Light Stimulation The SIM scanner system combines the main scanner with a laser light stimulation scanner. Control of the two independent beams enables simultaneous stimulation and imaging, to capture reactions during stimulation. Multi-stimulation software is used to continuously stimulate mu...
Page 19 - Photoconversion; Uncaging
Application/ Molecular Interaction Analysis Photoconversion The Kaede protein is a typical photoconvertible protein, which is a specialized fluorescent protein that changes color when exposed tolight of a specific wavelength. When the Kaede protein is exposed to laser light, its fluorescence changes...
Page 20 - Proprietary CO; Focal Drift Compensation for Long Time-Lapse Imaging
Significantly Improved Long Time-Lapse Throughput Equipped with motorized XY stage for repeated image acquisition from multiple points scattered across a wide area. The systemefficiently analyzes changes over time of cells in several different areas capturing, large amounts of data during a single e...
Page 21 - D Mosaic Imaging; Automated from 3D Image Acquisition to Mosaic Imaging; Mosaic Imaging for 3D XYZ Construction
Application/ Molecular Interaction Analysis 3D Mosaic Imaging Mosaic imaging is performed using a high-magnification objective to acquire continuous 3D (XYZ) images of adjacent fields of view using the motorized stage, utilizing proprietary software to assemble the images. The entire process from im...
Page 22 - Expandability to Support Diverse Application.
Expandability to Support Diverse Application. Application Standard Functions Optional Functions Molecular interaction and Intracellular diffusion measurement molecular concentration Calculation of diffusion coefficients for intracellular molecules, and analysis of analysis — molecular binding and ch...
Page 23 - mCherry
Expandability 350 350 300 300 400 400 450 450 500 500 550 550 600 600 650 650 700 700 750 750 800 800 405 LD 440 LD 473 LD 635 LD 55 9 LD 458 M ulti Argon 515 M ulti Argon 543 G re en HeNe 488 Argon mCherry 22
Page 24 - Laser Systems
Fluorescence Illumination Unit Stand with Mercury lamp house, motorized shutter,and fiber delivery system for conventional fluorescenceobservation. Light introduction via fiber optic port. Transmitted Light Detection Unit External transmitted light photomultiplier detector and100 W Halogen conventio...
Page 25 - Software
Expandability B C Fluorescence illumination unit *Optional unit Transmitted light detection unit IX81IX81-ZDC Inverted motorized microscope BX61WI BX61 Upright motorized microscope LD635 laser LD559 laser HeNeG laser AOTF Laser combiner (Single-fiber type) AOTF Laser combiner (Dual-fiber type) 635 n...
Page 26 - Objectives for fixed stage upright microscope; Objectives for BX2 and IX2
Objectives NA W.D. (mm) DIC prism Revolving nosepiece MPLN5X 0.10 20.00 – WI-SSNP, WI-SRE3 UMPLFLN10XW 0.30 3.50 WI-DIC10HR WI-SSNP, WI-SRE3 UMPLFLN20XW 0.50 3.50 WI-DIC20HR WI-SSNP, WI-SRE3 LUMPLFLN40XW 0.80 3.30 WI-DIC40HR WI-SSNP, WI-SRE3 LUMPLFLN60XW 1.00 2.00 WI-DIC60HR WI-SSNP, WI-SRE3 LUMFLN6...
Page 27 - Dimensions, Weight and Power Consumption; Images are courtesy of the following institutions:
Expandability Dimensions (mm) Weight (kg) Power consumption Microscope with scan unit BX61/BX61WI 320 (W) x 580 (D) x 565 (H) 41 — IX81 350 (W) x 750 (D) x 640 (H) 51 Fluorescence illumination unit Lamp 180 (W) x 320 (D) x 235 (H) 6.7 Power supply 90 (W) x 270 (D) x 180 (H) 3.0 AC 100-240 V 50/60 Hz...
Page 28 - FLUOVIEW website
FLUOVIEW website www.olympusfluoview.com • OLYMPUS CORPOARATION is ISO9001/ISO14001 certified. • Illumination devices for microscope have suggested lifetimes. Periodic inspections are required. Please visit our web site for details. • Windows is a registered trademark of Microsoft Corporation in the...
