Omega 25 - Manual

6 Working Conditions The thermal cycler is designed operate under the following conditions:  Indoor use  Ambient temperature range +5 o C to +40 o C  Altitude to 2000m  Relative humidity not exceeding 95%  Power supply fluctuations not exceeding 10%  Over voltage category II IEC 60364-4-443  ...

7 3. Cycler TCY Specification Temperature Temperature range 4°C to 99°C Temperature set point precision 0.1°C Block uniformity (over full range) ± 0.5°C Temperature accuracy ± 0.5°C End of program cooling below ambient Yes Heating/Cooling Rate Maximum heating rate 4.5ºC/s Minimum heating rate 0.1ºC/...

8 Dimensions Height 185mm Width 185mm Length 330mm Power Power consumption 230W Units marked 100V will work at any voltage between 90 and 110V. The performance will vary and will not necessarily meet the above typical specification at the extremes of voltage. 4. User operation and consumables Heated...

10 Switching on When you switch on the unit a screen similar to the one shown here will appear briefly. This indicates the software version and block type installed in the unit. When the initial screen has disappeared the screen shown here will automatically appear. There are three options: 1. ‘Prog...

11 5. Front panel controls The LCD Display The CYCLER TCY has a four line LCD display. The top three lines are information or program lines, while the bottom line is a prompt line. The active line flashes on screen and is shown in bold in this manual. The bottom line may change or flash between two ...

12 The keys The End, Finish or Exit key The “End” or “Finish” key is used to finish editing programs or to exit a program. It is also used to exit a sequence of key operations. The Up arrow key The “Up arrow” key is used for scrolling up through the lines on the screen. It is also used to increase c...

13 The Down arrow key The “Down arrow” key is used for scrolling down through the lines on the screen. It is also used to decrease certain fields when editing. The Decimal Point and Insert key The “Decimal Point” key is used when editing numeric fields. It is also used as an Insert key during progra...

14 In the prompt line is equivalent to the “End” key. On a program line means the program is locked; it can be copied and then edited but not edited directly. Up arrow Down arrow Enter Pause The following terms are used when programming the CYCLER TCY : Stage A ‘Stage’ is the name given to the main ...

15 Programming Options There are two options available for creating programs on the CYCLER: 1. Copy and edit one of the pre-written templates. 2. Create a new program from the defaults. Option 1: Creating programs using templates There are two pre-written basic PCR templates: a two-step and a three-...

18 To edit a temperature, type in the required temperature. In the example here, the heated lid temperature is being edited. Press “Enter” to accept. Use the “Down Arrow” and/or the “Up Arrow” keys to select the next field or step you wish to edit. To edit a field with on/off settings, first select ...

19 To copy a selected program If you select ‘Copy program’ a screen similar to this will appear and the software will automatically give the program the next number until it is named. Press “Enter” and type in a new name. You will then be able to edit the program as previously described. To delete a...

20 Example programs A couple of example thermal cycling programs are shown below. Typical 3-Step PCR Heated Lid 105 Preheat Lid on Pause off - - - - - - - - - - - - - - - - In denat 94°c 5min Hot Start off - - - - - - Stage 1 - - - - - - Number Cycles 30 Step 94°c 0m30 max Step 55°c 0m30 max step 72...

22 To add a new stage before an existing one Use the “Up Arrow” or “Down Arrow” keys to highlight the stage number. Press "Enter" A new stage will be added before the existing stage. The other stage numbers will be adjusted as appropriate. The new stage can now be edited as described above. ...

23 To insert a ‘pause’ into a program To insert a pause after an existing stage, follow the steps for inserting a stage as above. Highlight the ‘Number cycles’ line then press “Pause” The prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’. Press “Enter” to insert a pause. The stage nu...

24 To program increment or decrement temperature or time. Under normal circumstances, the Hold Temperature of all steps is constant. However, it is possible to automatically increment or decrement the temperature of a specified step of a programme. Temperature profile of incremented Hold Temperature...

27 To run a program When the unit is switched on the display will show this screen. If known, type in the ‘Speed dial’ number for the required program and press “Enter” This will take you directly to the required program. If you do not know the ‘Speed dial’ number, press “Enter” to access the list o...

29 Program Information Pressing “Enter” while a program is running will give information about the current run including which program is actually running and the unit status e.g. holding or ramping. An example is shown here. 7. Information The Information field on the front screen contains all the ...

30 Access to the ‘Configuration’ set up is password protected. Default admin passwords is 1234 To enter configuration, use the alpha keys to type in ‘1234’ Press “Enter” There are a number of options which can be changed; these are listed here. Use the “Up Arrow” and “Down Arrow” keys to select then...

31 When you have finished, press the “Enter” key to go back to the ‘Configuration’ menu. Passwords The ‘Passwords’ function allows the administrator to change the ‘Admin password’ (which allows access to Configuration) and also to delete any passwords associated with programs. Highlight ‘Passwords’ ...

32 9. Notes on transferring protocols from other thermal cyclers The CYCLER TCY heats at over 3.6°C/s and provides the highest levels of temperature uniformity. Many slower machines have historically begun the countdown on hold times before the set temperature is reached in order to avoid any oversh...

34 11. Some frequently asked questions Q1 What is the ‘Pause’ function at the start of the program used for? A1 Some users prefer to preheat the heated lid before placing the samples into the unit. The pause feature is used to stop the unit a fter the 4 minut e heated lid preheat step. It will also ...

35 12. Additional information Brief fault finding notes and a list of replacement parts are given in this section. Note that this equipment should only be dismantled by properly trained personnel. Removing the outer cover exposes potentially lethal power voltages. There are no user serviceable parts...

WARRANTY/ DISCLAIMER OMEGA ENGINEERING, INC. warrants this unit to be free of defects in materials and workmanship for a period of 36 months from date of purchase. OMEGA’s WARRANTY adds an additional one (1) month grace period to normal three (3) year product warranty to cover handling and shipping ...

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