Learning Resources LER2583 - Manual
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Agar
Preparation & Use
Agar is a gelatinous material extracted
from certain marine algae. It is used for
gelling foods and as a bacteria culture
medium. Nutrient agar will resemble
a solution something like Jell-O
®
with
beef soup. It is not edible and should
not be ingested.
Contents:
2 plastic petri dishes
2 packets of Nutrient Agar
(Use one packet per petri dish)
Instructions:
1. Boil 2 tablespoons of distilled water.
2. Add entire contents of one package of
Nutrient Agar to the boiling water.
3. Mix
solution
until
the
agar
is
completely dissolved. The solution
will start to resemble a jelly mixture.
4. Pour the agar solution into one
petri dish.
5. Refrigerate to gel (about 30 minutes).
6. After the solution turns into a waxy
gel, turn the dish over and draw
pie-cut sections on the bottom and
up the sides. Number each section.
7. Re-draw the sections on paper to
record which sample is in each section.
8. Next, use a clean toothpick to collect
a sample of what you want to test by
rubbing it on the sample’s surface,
then rub it on one section of the agar.
9. Cover the dish and leave it in a warm,
draft-free area for 24 to 48 hours.
10. Leaving the dish open and exposed
to the air is another way to collect
samples floating around on the
breeze. You can even sneeze on
the
gel
and
see
what
grows.
11. By turning the dish upside down it
allows for less moisture to collect.
12. It's best to use a magnifier to
see the results. See your dealer
for
various
magnifiers
available
from Learning Resources.
WARNING! NOT A FOOD PRODUCT.
DO NOT INGEST.
LER2583
Ages 8+
© Learning Resources, Inc., Vernon Hills, IL (U.S.A.)
Learning Resources Ltd., King’s Lynn, Norfolk (U.K.)
Please retain our address for future reference.
Agar made in USA
LRM 2583-TG
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