Fujifilm LAS-3OOO - Manual

Specifications and Applications

Fuji Photo Film Co., Ltd.

26-30, Nishiazabu 2-Chome, Minato-ku, Tokyo 106-8620, Japan, Tel: +81-3-3406-2201, Fax: +81-3-3406-2158 • http://lifescience.fujifilm.com • E-mail: [email protected]

Fujifilm Medical Systems U.S.A., Inc.

419 West Avenue, Stamford, CT 06902, U.S.A., Tel: +1-203-324-2000 ext. 6112 (1-800-431-1850 ext. 6112 in the U.S.), Fax: +1-203-351-4713 • http://www.fujimed.com • E-mail: [email protected]

Fuji Photo Film (Europe) GmbH

, Heesenstr. 31, 40549 Düsseldorf, Germany, Tel: +49-211-5089-174, Fax: +49-211-5089-139 • http://www.fujifilm.de • E-mail: [email protected]

Specifications and system configuration subject to change for improvement without notice. All other product names mentioned herein are the trademarks of their respective owners.

Re

f.

No

. B

B-

20

4E

(0

4,

09

)

LAS-3OOO

S u p e r C C D

R e m o t e C o n t r o l l e d

S c i e n c e I m a g i n g S y s t e m

Image Capturing Unit (Camera Head and Controller)

CCD chip

Fujifilm Super CCD Area Type chip

Number of pixels

3.2M pixels

Pixel size 10.75 x 10.75µm
Cooling

Two-stage thermoelectric module with air circulation

Cooling temperature

-30°C (when room is below 28°C)

Focusing

Power focusing; remote and preset control

Exposure time

1/100 second to 30 hrs (2 hrs to 30 hrs to be set manually)

Dynamic range

Four orders of magnitude

Gradation

16 bits (65,536)

Image size

12.6MB Max.; 49.2KB Min.

Maximum sample size

25 x 25cm (wide angle lens); 14 x 21cm (Fujinon VRF43LMD lens)

Binning

1 x 2, 2 x 4, 4 x 8, and 8 x 16 pixels

Interface

USB1.1

Software

Image capture

Fujifilm Image Reader (Mac

™

and Windows

®

)

Image analysis

Fujifilm Image Gauge (Mac

™

); Fujifilm MultiGauge (Windows

®

)

Dimensions and Weights

Camera head

180 (W) x 170 (H) x 250mm (D)

3.4kg

Dark Box IV

510 (W) x 730 (H) x 480mm (D)

49.0kg

Analyzing Unit

Operating system

Windows

®

XP or Mac

™

OS 9 & X

Applicable Reagents and/or Samples

Chemiluminescence

CDP-Star

®

, ECL

™

, ECL Plus

™

, SuperSignal, ImmunoStar, CSPD

®

Fluorescence

EtBr (W/UV light), SYBR

®

Green I & II, SYPRO

®

Orange,

GFP, DY-458XL (blue LED)

Pro-Q

™

Diamond, Cy

™

3, RFP, DY-520XL (green LED), Alexa Fluor 633,

Cy

™

5, DY-647 (red LED)

Chemifluorescence

*AttoPhos

™

Digitization

CBB-stained gel, Silver stained gel (Transilluminator)

NBT/BCIP-stained membrane. MW marker (Epi-illuminator)

Intelligent Dark Box IV

EPI-illuminator for fluorescence

blue LED (470nm), green LED (520nm), red LED (630nm)

EPI-illuminator for digitization

White-light source

Transilluminator - for digitization of

stained gels and autoradiographic films

White LED

UV transilluminator**

UV-light source (312nm)

Filter turret

Five positions

Printers

Pictrography 3500

Lens

Lens

High-sensitivity lens (FUJINON VRF43LMD)

Wide-view lens

F-number

0.85

F-number

2.0

Focal length

43mm

Focal length

24mm

Focus

Remote power focusing

Focus

Manual

Mount

Bayonet

Mount

Adapter to Nikon F mount

Analysis

Set a tray

Remote controlled

focusing

Exposure

Sample is placed on a tray

Chemiluminescence

Fluorescence

Chemifluorescence

Digitization

Chemiluminescence Mode

Fluorescence

Chemifluorescence Mode

Digitize Mode

Sample

Filter

blue LED

green LED

red LED

UV trans-

illuminator

White light

epi-illuminator

White trans-

illuminator

The image analysis process captures images in three modes:
chemiluminescence, fluorescence/chemifluorescence and digitization.

The binning mode of the LAS-3000 allows researchers to select from four
binning settings to enhance both imaging sensitivity and image resolution.

*No license is granted for use of AttoPhos™ to detect nucleic acid on a nylon membrane.
**No license is granted for pre-labeling gel electrophoresis method with the UV transilluminator.