Page 3 - Warranty and Special Provisions Limited Warranty
Warranty and Special Provisions Limited Warranty DYNEX Technologies products are fully guaranteed for one year against defects in parts, materials, and workmanship. Defective parts and materials will be replaced or, at the discretion of DYNEX Technologies, repaired at no charge for a period of one y...
Page 5 - Table of Contents
Table of Contents Table of Contents About this Manual iii Chapter 1 Introduction 1 Description 1 Programming 2 Wash Head 3 Wash Head Positions 4 Wash Fluid 5 Waste 5 Features 7 Specifications 9 Warning Labels 11 Chapter 2 Installation 13 Unpacking 13 Materials Provided 13 Hardware Components 15 Posi...
Page 6 - Chapter 7 Troubleshooting
Table of Contents Cleaning and Decontamination 69 Cleaning the Containers 70 Replacing the Tubing 71 Pump Calibration 74 Chapter 7 Troubleshooting 77 Operational Problems 77 Chapter 8 Service 81 Service Procedures 81 Spare Parts 82 Returning a Washer for Service 83 Contact DYNEX Technologies 85 Inde...
Page 7 - About this Manual; This manual gives you the information needed to:
About this Manual About this Manual This manual has been written for laboratory technicians and provides detailed instructions for using the Opsys MW™ system. This manual gives you the information needed to: • Review safety precautions. • Install the Opsys MW™ system. • Understand the Opsys MW™ menu...
Page 8 - iv
About this Manual This page is intentionally left blank iv Opsys MW™ User’s Guide
Page 9 - Chapter 1 Introduction; Description
Description Chapter 1 Introduction The Opsys MW™ is a microprocessor-controlled microplate washing system that performs wash protocols that are defined by the user. It is designed to wash all of the wells in one column or one row of a 96-well plate at once. The washing protocol can be programmed so ...
Page 10 - Programming
Description Programming All wash protocols on the Opsys MW™ Microplate Washer are user-defined. Up to 40 wash protocols can be contained on the system. Note: Memory limitations may restrict the size and complexity of an individual wash protocol. In addition, the system can be configured with up to t...
Page 11 - wash pins; Wash Head
Description Wash Head The wash head on the Opsys MW™ Microplate Washer contains multiple sets of dual wash pins . Each set of dual wash pins consists of a dispense pin and an aspirate pin that are closely spaced so that fluid can be aspirated from and dispensed into a well simultaneously. The follow...
Page 12 - Wash Head Positions
Description Wash Head Positions The vertical positions that the wash head can assume are described below. Each wash head position can be specified by the user to within 0.1 mm. Note : Procedures for specifying wash head positions for various plate types are described on page 30. Wash Head Position D...
Page 13 - Wash Fluid; Waste; aspiration
Description Wash Fluid A single wash fluid is used on the Opsys MW™ Microplate Washer. The wash fluid is contained in the Wash Container and is dispensed into the wells whenever a Dispense or Fill operation is specified in the wash protocol. In addition, wash fluid is dispensed into the purge tray d...
Page 15 - Features
Features Features The Opsys MW™ system has a number of performance and convenience features. These are summarised below: • Small footprint • Up to 40 wash protocols can be programmed • Up to ten plate types can be programmed • Two wash head configurations (1 x 8 and 1 x 12) • On-board self diagnosti...
Page 17 - Specifications; Dimensions and Weight
Specifications Specifications Dimensions and Weight Length 49 cm (19.3 in) Width 38 cm (15.0 in) Height 19 cm (7.5 in) Weight 9.1 kg (20 lbs) Operation Dispense Range 50 – 999 µ L Dispense Precision 5% CV (with 300 µ L distilled water) Residual Aspirate Volumes < 1 µ L for U and V bottom plates &...
Page 18 - Input Ranges; Standards
Specifications Input Ranges Assay Programming Assay Name Up to 10 characters Password Up to 8 characters Move: Start Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: End Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: #Cycles 1 - 9 (Increment:...
Page 19 - Warning Labels; Label Description
Warning Labels Warning Labels The Opsys MW™ or its components may contain certain labels that that either warn the user of a hazard or note an electrical connection. The labels that may be used on the Opsys MW™ system are described below. Label Description Alternating current is present. ( English )...
Page 21 - Chapter 2 Installation; Materials Provided
Unpacking Chapter 2 Installation Unpacking Materials Provided The main carton contains the instrument. The contents of this carton are listed below: Article Quantity Opsys MW™ System 1 Instrument Power Cable 1 Cleaning Wire, Aspirate 1 Cleaning Wire, Dispense 1 Instrument Configuration Report 1 User...
Page 22 - To unpack the components:
Unpacking To unpack the components: 1. Place the Opsys MW™ instrument in the position where it will be located for use. 2. Place the Wash Container, Waste Container and Foam/Aerosol Trap at the rear of the instrument. 3. Examine the packaging to be sure that the power cord and other materials have b...
Page 23 - Hardware Components
Hardware Components Opsys MW™ User’s Guide 15 Hardware Components Hardware components of the Opsys MW™ instrument are shown in Figure 2 and described below. Note: The Wash Container, Waste Container and Foam/Aerosol Trap are external to the instrument and are not shown in Figure 2. Wash Head Microti...
Page 24 - Keypad; Wash Container; Waste Container
Hardware Components • Keypad . The keypad is used by the operator to select commands and enter wash parameters. • RS232 Interface . The system includes an RS232 interface so that an external computer can be connected for technical service purposes. • Wash Container . (Shown in Figure 1). The wash co...
Page 26 - Positioning the Instrument
Positioning the Instrument Positioning the Instrument Determine the area where the system will be located. You will need a firm and level area that is approximately 51 cm (20 inches) wide, 62 cm (24 inches) deep, and 40 cm (16 inches) high for the Opsys MW™. 18 Opsys MW™ User’s Guide
Page 27 - Installing the Wash Head
Installing the Wash Head Installing the Wash Head The Wash Head must be located on the Wash Head Arm. To install the Wash Head: 1. Position the Arm over the Purge Reservoir portion of the Plate Holder. 2. Place the Wash Head on the two Arm Pins, being careful to properly seat the Wash Head on the su...
Page 28 - Installing the Wash Container; To install the Wash Container:
Installing the Wash Container Installing the Wash Container The dispense tubing and dispense pump power cable must be connected. To install the Wash Container: 1. Fill the Wash Container with the wash solution that is to be used. Note : The Wash Container contains up to 4 liters. 2. Insert the Pick-...
Page 29 - Installing the Waste Container; To install the Waste Container and Foam/Aerosol Trap:
Installing the Waste Container Installing the Waste Container To install the Waste Container and Foam/Aerosol Trap: 1. Insert the Quick-Connect end of the vacuum tubing on the Foam/Aerosol Trap to the Quick-Connect vacuum connector at the rear of the instrument Figure 3). 2. Screw on the Waste Conta...
Page 30 - Turning on the System
Turning on the System Turning on the System Note : Depending upon local electrical codes, an uninterruptible power system (UPS) may be required in your laboratory. A UPS is not provided with the Opsys MW™ system. 1. Connect the Opsys MW™ to the laboratory electrical supply outlet. CAUTION: The Opsys...
Page 31 - Chapter 3 Configuration; scroll keys
The Keypad Opsys MW™ User’s Guide 23 Chapter 3 Configuration The Keypad The keypad is used to access the menus and commands for setting up the Opsys MW™ entering new wash protocols and running the wash protocols. The keypad contains scroll keys , function keys , and alphanumeric keys . The keys on t...
Page 33 - Key
The Keypad Key Purpose Alphanumeric Keys The numeral shown on the key is displayed by pressing the key once. If the key is pressed two to seven times rapidly, one of the letters on the key will be displayed. The letter that is displayed depends upon the number of times the key is pressed. Opsys MW™ ...
Page 34 - Menus; Menu
Menus Menus The Opsys MW™ menus are hierarchical and are organised by function. There are two main menus: Setup and Program. Menu Purpose SETUP Configure the Instrument PROGRAM Create or edit plate washing protocols * * A protocol includes general information (e.g. the type of microplate to be washe...
Page 35 - The SETUP Menu
Menus The SETUP Menu Press the function key under SETUP to display the SETUP menu (Figure 5). The SETUP menu contains commands to configure the instrument. 1: TESTNAME SETUP PROGRAM Select Option VERSION CAL. Select Option HEAD PLATE Figure 5. SETUP Menu The PROGRAM Menu Press the function key under...
Page 36 - Configuring the System
Configuring the System Configuring the System The Opsys MW™ must be configured before it is operated for the first time. The commands for doing this are contained in the SETUP menu (Figure 7). Use ARROW keys to scroll through the menus. 1: TESTNAME SETUP PROGRAM Select Option VERSION CAL. Select Opt...
Page 37 - Specifying the Wash Head
Configuring the System Specifying the Wash Head The HEAD command allows the installed wash head to be specified: 1. Select SETUP. The display will read: Select option HEAD PLATE 2. Select HEAD. The current wash head selection is displayed in the upper line: Wash head? 8 8 If needed, scroll right to ...
Page 38 - Specifying Plate Parameters; SHOW
Configuring the System Specifying Plate Parameters The PLATE command allows plate parameters to be specified for up to 10 plate types. When setting plate parameters, a default value is displayed for each of the wash head positions. The default values are factory-set and represent approximate startin...
Page 39 - To specify parameters for a plate type:
Configuring the System To specify parameters for a plate type: 1. Obtain an empty plate of the plate type being specified and place it in the plate holder. 2. Select SETUP (if needed). The display will read: Select option HEAD PLATE 3. Select PLATE. The name of the first plate is displayed in the lo...
Page 43 - Displaying the Software Version
Configuring the System Displaying the Software Version The VERSION command displays the software version that is currently installed on the Opsys MW™ instrument: 1. Select SETUP (if needed). The display will read: Select option HEAD PLATE 2. Scroll right to display the next selection: Select option ...
Page 44 - Specifying the Pump Parameters
Configuring the System Specifying the Pump Parameters The CAL. command allows the calibration values for each pump to be specified: 1. Select SETUP (if needed). The display will read: Select option HEAD PLATE 2. Scroll right to display the next selection: Select option VERSION CAL. 3. Select the pum...
Page 45 - Selecting Clean
Configuring the System Selecting Clean The CLEAN command enables the washer to be cleaned automatically at a specified interval. 1. Scroll right to display the next section: Select option CLEAN 2. Select CLEAN. The display will read: Auto Clean? YES NO 3. Select YES if you want periodic automatic cl...
Page 47 - Chapter 4 Wash Protocols
Wash Protocol Operations Chapter 4 Wash Protocols Wash Protocol Operations A wash protocol consists of a series of Purge , Move , Soak , Aspirate , Dispense and Fill operations. Purge , Move and Soak can be carried out in any sequence. Aspirate , Dispense and Fill can only be carried out within a Mo...
Page 48 - Operation; Soak
Wash Protocol Operations Operation Description Dispense Dispenses a specified amount of fluid into the wells after aspirating the contents of the wells (i.e. wherever a DISPENSE step is added to a protocol, the OPSYS MW will automatically aspirate the wells before dispensing more fluid in order to p...
Page 49 - Creating a New Wash Protocol; Specifying the Header; NOTE
Creating a New Wash Protocol Creating a New Wash Protocol Specifying the Header NOTE: Pressing CANCEL while creating or editing a protocol will bring you back to the main menu without saving your changes. or (or use keypad) Select one of the 40 protocol numbers. ↓ PROGRAM Select PROGRAM from the mai...
Page 50 - To create a new wash protocol header:
Creating a New Wash Protocol To create a new wash protocol header: 1. Display the main menu. 1: - - - - - - - - - - SETUP PROGRAM 2. Use the up or down scroll keys to display the protocol number (or use the keypad to enter the protocol number) that is to be created: 6: - - - - - - - - - - SETUP PROG...
Page 53 - Specifying Wash Cycle Operations; Dispense
Creating a New Wash Protocol Specifying Wash Cycle Operations The final step in creating a wash protocol is to specify individual operations within the wash protocol. An example of a wash protocol is shown in 45. In this example, the dispense wash pins are purged, and then a Dispense and Aspirate ar...
Page 55 - To specify a Move cycle:
Creating a New Wash Protocol To specify a Move cycle: 1. Display the header information for the selected wash protocol, as outlined on page 41. The display will read: Select option MOVE PURGE 2. Select MOVE. The display will read: Start strip? 1 3. Using the keypad, enter the strip number where the ...
Page 57 - YES
Creating a New Wash Protocol To specify a Dispense (within a Move): Note : A Dispense or Aspirate must be performed within a Move cycle. 1. Select DISPENSE. The display will read: Fluid volume? 350 2. Use the keypad to enter the volume of fluid to be dispensed. Note : A volume ranging from 50 to 999...
Page 61 - Ending a MOVE Cycle
Creating a New Wash Protocol Ending a MOVE Cycle 1. Scroll right to display the next selection. The display will read: Select option SOAK FILL 2. Scroll right to display the next selection. The display will read: Select option END LOOP 3. Select END LOOP. The display will read: Select option MOVE PU...
Page 63 - To specify the End of the wash protocol:
Creating a New Wash Protocol To specify the End of the wash protocol: 1. Scroll right to display the next selection. The display will read: Select option SOAK END 2. Select END. The display will read: 1: NAME_01 SETUP PROGRAM Opsys MW™ User’s Guide 55
Page 64 - Modifying a Wash Protocol; To modify a wash protocol:
Modifying a Wash Protocol Modifying a Wash Protocol An existing wash protocol can be modified by using many of the same commands that are used to create a wash protocol. To modify a wash protocol: 1. Display the main menu: 1: NAME_01 SETUP PROGRAM 2. Use the up and down scroll keys to select the was...
Page 66 - To modify a wash operation:
Modifying a Wash Protocol To modify a wash operation: 1. Select EDIT while the operation is displayed. The first step in the operation is displayed: Start strip? 1 Cycles Note : The actual step that is displayed depends upon the operation being modified. 2. Modify the operation, using the same proce...
Page 67 - To delete a wash operation:
Modifying a Wash Protocol To delete a wash operation: 1. Select DELETE while the operation is displayed. The operation is removed from the wash protocol. Note : If a MOVE operation is deleted, all operations within the MOVE (i.e., Aspirate , Dispense , Soak and Fill ) are also removed. Opsys MW™ Use...
Page 68 - To insert a wash operation:
Modifying a Wash Protocol To insert a wash operation: 1. Scroll right while the operation that the new operation is to precede is displayed: MOVE 1-10 1 Cycles INSERT Note : The inserted operation will take place immediately before the operation that is displayed. 2. Select INSERT. The display will ...
Page 69 - Copying a Wash Protocol; To copy a wash protocol:
Copying a Wash Protocol Copying a Wash Protocol An existing wash protocol can be copied to another program number. If a wash protocol is already in that location, it will be overwritten. To copy a wash protocol: 1. Display the main menu: 1: NAME_01 SETUP PROGRAM 2. Use the up and down scroll keys to...
Page 71 - Chapter 5 Washing a Plate; To empty the waste container:
Preparation Chapter 5 Washing a Plate Once the parameters for at least one plate type and one wash protocol have been set, the system can be used to wash a plate. Preparation The only preparation that is required is to check the fluid levels in the Wash Container and the Waste Container. Service the...
Page 72 - To refill the wash container:
Preparation To refill the wash container: 1. Separate the dispense line quick-connector in the cap of the Wash Container. 2. Unscrew the cap of the Wash Container and lay it and the Pick- Up Filter on a clean paper towel. 3. Fill the Wash Container with the wash fluid that is being used. Note : If t...
Page 73 - Washing a Plate
Washing a Plate Washing a Plate A plate is washed by positioning it on the carrier block, selecting the wash protocol to be run, and pressing the START key. To wash a plate: 1. (If needed) Turn on the instrument. The display will read: 1: TESTNAME SETUP PROGRAM 2. Place the microplate on the plate c...
Page 75 - Chapter 6 Routine Maintenance; Empty the Wash Container and clean it.; Empty the Waste Container and clean it.; Six month maintenance:
Routine Maintenance Procedures Chapter 6 Routine Maintenance Routine Maintenance Procedures The Opsys MW™ Microplate Washer is constructed from materials that resist chemical attack. If a spill occurs, it should be cleaned up as soon as possible using a cloth moistened with a mild laboratory deterge...
Page 76 - As required maintenance:
Routine Maintenance Procedures • Replace the dispense (Wash) tubing. • Replace the aspirate (Waste) tubing. Note : The dispense tubing and aspiration tubing may need to be replaced more frequently than every six months, depending upon the frequency of use and the severity of operating conditions. Co...
Page 77 - Cleaning and Decontamination; To clean the system:; To decontaminate the system:
Cleaning and Decontamination Cleaning and Decontamination The Opsys MW™ is constructed from materials that resist chemical attack. Spills should be cleaned as soon as possible. If you need to decontaminate the Opsys MW™ instrument (for example, before servicing the instrument), clean the system and ...
Page 78 - Cleaning the Containers; To empty and clean the waste container:
Cleaning the Containers Cleaning the Containers Procedures for emptying, cleaning and refilling the Wash Container and the Waste Container are described below. To empty and clean the waste container: 1. Unscrew the cap of the Waste Container. Lay the cap and level sensor assembly on a paper towel. 2...
Page 79 - Replacing the Tubing; To replace the dispense tubing:
Replacing the Tubing Replacing the Tubing To replace the dispense tubing: 1. Separate the dispense line quick-connector in the cap of the Wash Container. Unscrew the cap, lay it and the Pick-Up Filter on a clean paper towel. 2. Discard the contents of the Wash Container. Thoroughly rinse the contain...
Page 80 - To replace the vacuum tubing:
Replacing the Tubing To replace the vacuum tubing: Note : This procedure describes replacement of the vacuum tubing connecting the Waste Container to the Foam/Aerosol Trap. The vacuum tubing connecting the Foam/Aerosol Trap to the rear of the instrument should not require changing. 1. Unscrew the Ca...
Page 81 - To replace the waste tubing:
Replacing the Tubing To replace the waste tubing: 1. Unscrew the Cap of the Waste Container and lay it with the level sensor assembly on a clean paper towel. 2. Discard the contents of the Waste Container in accordance with local regulations. Thoroughly rinse the container with deionized water and l...
Page 82 - Pump Calibration; Calibration Protocol:
Pump Calibration Pump Calibration Each Opsys MW™ washer manifold, or head, (e.g., 1 x 8, 1 x 12) has three calibration values stored in memory. These values are calculated and stored during manufacture at DYNEX Technologies. When the Opsys MW configuration is changed from one manifold to another, th...
Page 85 - Symptom
Operational Problems Chapter 7 Troubleshooting Operational Problems Problems that may occur during normal operation of the Opsys MW™ are described below. For each problem, the symptom, probable cause and the means for resolving the problem are shown. Symptom Probable Cause Resolution The instrument ...
Page 89 - Chapter 8 Service; To remove the wash head assembly:
Service Procedures Chapter 8 Service Service Procedures The only user service procedures that can be carried out for the Opsys MW™ are listed below: • Removal and replacement of the wash head assembly • Cleaning of the wash head assembly To remove the wash head assembly: 1. Loosen the two thumbscrew...
Page 90 - Spare Parts; Accessories
Spare Parts Spare Parts Accessories 13001580 Plate Holder Assembly 13001610 8-Way Head Assembly 13001630 12-Way Head Assembly 352104000 Cleaning Wire, 0.040” 352101800 Cleaning Wire, 0.018” 91000051 User Manual 91000070 OPSYS MW Quick Set Up Instructions 23501800 Bottle Wide Mouth (2120-0010, Nalgen...
Page 91 - Returning a Washer for Service; To return a Washer for service:
Returning a Washer for Service Returning a Washer for Service If the instrument must be returned for service, it must be cleaned and decontaminated if it has been in contact with potentially infectious body fluids (including human blood), pathological samples, or toxic or radioactive materials. Note...
Page 92 - EQUIPMENT IN TRANSIT
Returning a Washer for Service EQUIPMENT IN TRANSIT IMPORTANT: Please include a copy of this form with each instrument. If your instrument contains a hard drive, please retain back-up copies of any stored files. Failure to do so may result in the loss of those files . Return Authorization Number: __...
Page 93 - Contact DYNEX Technologies
Contact DYNEX Technologies Contact DYNEX Technologies DYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151 USA Tel: 703-631-7800 Toll Free: 800-288-2354 Fax: 703-803-1441 www.dynextechnologies.com Opsys MW™ User’s Guide 85
Page 94 - Index
Index Index as required maintenance, 68 ASPIRATE, 39 aspiration height, 4 entering, 32 bottom wash specifying, 34 bottom wash height, 4 CAL. command, 36 calibration prototcol, 74 cleaning, 69 Wash Container, 70 Waste Container, 70 configuration, 28 connections, 17 daily maintenance, 67 data bits, 9 ...
