Dynex 91000051 - Manuals

About this Manual About this Manual This manual has been written for laboratory technicians and provides detailed instructions for using the Opsys MW™ system. This manual gives you the information needed to: • Review safety precautions. • Install the Opsys MW™ system. • Understand the Opsys MW™ menu...

About this Manual This page is intentionally left blank iv Opsys MW™ User’s Guide

Description Chapter 1 Introduction The Opsys MW™ is a microprocessor-controlled microplate washing system that performs wash protocols that are defined by the user. It is designed to wash all of the wells in one column or one row of a 96-well plate at once. The washing protocol can be programmed so ...

Description Programming All wash protocols on the Opsys MW™ Microplate Washer are user-defined. Up to 40 wash protocols can be contained on the system. Note: Memory limitations may restrict the size and complexity of an individual wash protocol. In addition, the system can be configured with up to t...

Description Wash Head The wash head on the Opsys MW™ Microplate Washer contains multiple sets of dual wash pins . Each set of dual wash pins consists of a dispense pin and an aspirate pin that are closely spaced so that fluid can be aspirated from and dispensed into a well simultaneously. The follow...

Description Wash Head Positions The vertical positions that the wash head can assume are described below. Each wash head position can be specified by the user to within 0.1 mm. Note : Procedures for specifying wash head positions for various plate types are described on page 30. Wash Head Position D...

Description Wash Fluid A single wash fluid is used on the Opsys MW™ Microplate Washer. The wash fluid is contained in the Wash Container and is dispensed into the wells whenever a Dispense or Fill operation is specified in the wash protocol. In addition, wash fluid is dispensed into the purge tray d...

Features Features The Opsys MW™ system has a number of performance and convenience features. These are summarised below: • Small footprint • Up to 40 wash protocols can be programmed • Up to ten plate types can be programmed • Two wash head configurations (1 x 8 and 1 x 12) • On-board self diagnosti...

Specifications Specifications Dimensions and Weight Length 49 cm (19.3 in) Width 38 cm (15.0 in) Height 19 cm (7.5 in) Weight 9.1 kg (20 lbs) Operation Dispense Range 50 – 999 µ L Dispense Precision 5% CV (with 300 µ L distilled water) Residual Aspirate Volumes < 1 µ L for U and V bottom plates &...

Specifications Input Ranges Assay Programming Assay Name Up to 10 characters Password Up to 8 characters Move: Start Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: End Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: #Cycles 1 - 9 (Increment:...

Warning Labels Warning Labels The Opsys MW™ or its components may contain certain labels that that either warn the user of a hazard or note an electrical connection. The labels that may be used on the Opsys MW™ system are described below. Label Description Alternating current is present. ( English )...

Unpacking Chapter 2 Installation Unpacking Materials Provided The main carton contains the instrument. The contents of this carton are listed below: Article Quantity Opsys MW™ System 1 Instrument Power Cable 1 Cleaning Wire, Aspirate 1 Cleaning Wire, Dispense 1 Instrument Configuration Report 1 User...

Unpacking To unpack the components: 1. Place the Opsys MW™ instrument in the position where it will be located for use. 2. Place the Wash Container, Waste Container and Foam/Aerosol Trap at the rear of the instrument. 3. Examine the packaging to be sure that the power cord and other materials have b...

Hardware Components Opsys MW™ User’s Guide 15 Hardware Components Hardware components of the Opsys MW™ instrument are shown in Figure 2 and described below. Note: The Wash Container, Waste Container and Foam/Aerosol Trap are external to the instrument and are not shown in Figure 2. Wash Head Microti...

Hardware Components • Keypad . The keypad is used by the operator to select commands and enter wash parameters. • RS232 Interface . The system includes an RS232 interface so that an external computer can be connected for technical service purposes. • Wash Container . (Shown in Figure 1). The wash co...

Positioning the Instrument Positioning the Instrument Determine the area where the system will be located. You will need a firm and level area that is approximately 51 cm (20 inches) wide, 62 cm (24 inches) deep, and 40 cm (16 inches) high for the Opsys MW™. 18 Opsys MW™ User’s Guide

Installing the Wash Head Installing the Wash Head The Wash Head must be located on the Wash Head Arm. To install the Wash Head: 1. Position the Arm over the Purge Reservoir portion of the Plate Holder. 2. Place the Wash Head on the two Arm Pins, being careful to properly seat the Wash Head on the su...

Installing the Wash Container Installing the Wash Container The dispense tubing and dispense pump power cable must be connected. To install the Wash Container: 1. Fill the Wash Container with the wash solution that is to be used. Note : The Wash Container contains up to 4 liters. 2. Insert the Pick-...

Installing the Waste Container Installing the Waste Container To install the Waste Container and Foam/Aerosol Trap: 1. Insert the Quick-Connect end of the vacuum tubing on the Foam/Aerosol Trap to the Quick-Connect vacuum connector at the rear of the instrument Figure 3). 2. Screw on the Waste Conta...

Turning on the System Turning on the System Note : Depending upon local electrical codes, an uninterruptible power system (UPS) may be required in your laboratory. A UPS is not provided with the Opsys MW™ system. 1. Connect the Opsys MW™ to the laboratory electrical supply outlet. CAUTION: The Opsys...

The Keypad Opsys MW™ User’s Guide 23 Chapter 3 Configuration The Keypad The keypad is used to access the menus and commands for setting up the Opsys MW™ entering new wash protocols and running the wash protocols. The keypad contains scroll keys , function keys , and alphanumeric keys . The keys on t...

The Keypad Key Purpose Alphanumeric Keys The numeral shown on the key is displayed by pressing the key once. If the key is pressed two to seven times rapidly, one of the letters on the key will be displayed. The letter that is displayed depends upon the number of times the key is pressed. Opsys MW™ ...

Menus Menus The Opsys MW™ menus are hierarchical and are organised by function. There are two main menus: Setup and Program. Menu Purpose SETUP Configure the Instrument PROGRAM Create or edit plate washing protocols * * A protocol includes general information (e.g. the type of microplate to be washe...

Menus The SETUP Menu Press the function key under SETUP to display the SETUP menu (Figure 5). The SETUP menu contains commands to configure the instrument. 1: TESTNAME SETUP PROGRAM Select Option VERSION CAL. Select Option HEAD PLATE Figure 5. SETUP Menu The PROGRAM Menu Press the function key under...

Configuring the System Configuring the System The Opsys MW™ must be configured before it is operated for the first time. The commands for doing this are contained in the SETUP menu (Figure 7). Use ARROW keys to scroll through the menus. 1: TESTNAME SETUP PROGRAM Select Option VERSION CAL. Select Opt...

Configuring the System Specifying the Wash Head The HEAD command allows the installed wash head to be specified: 1. Select SETUP. The display will read: Select option HEAD PLATE 2. Select HEAD. The current wash head selection is displayed in the upper line: Wash head? 8 8 If needed, scroll right to ...

Configuring the System Specifying Plate Parameters The PLATE command allows plate parameters to be specified for up to 10 plate types. When setting plate parameters, a default value is displayed for each of the wash head positions. The default values are factory-set and represent approximate startin...

Configuring the System To specify parameters for a plate type: 1. Obtain an empty plate of the plate type being specified and place it in the plate holder. 2. Select SETUP (if needed). The display will read: Select option HEAD PLATE 3. Select PLATE. The name of the first plate is displayed in the lo...

Configuring the System Displaying the Software Version The VERSION command displays the software version that is currently installed on the Opsys MW™ instrument: 1. Select SETUP (if needed). The display will read: Select option HEAD PLATE 2. Scroll right to display the next selection: Select option ...

Configuring the System Specifying the Pump Parameters The CAL. command allows the calibration values for each pump to be specified: 1. Select SETUP (if needed). The display will read: Select option HEAD PLATE 2. Scroll right to display the next selection: Select option VERSION CAL. 3. Select the pum...

Configuring the System Selecting Clean The CLEAN command enables the washer to be cleaned automatically at a specified interval. 1. Scroll right to display the next section: Select option CLEAN 2. Select CLEAN. The display will read: Auto Clean? YES NO 3. Select YES if you want periodic automatic cl...

Wash Protocol Operations Chapter 4 Wash Protocols Wash Protocol Operations A wash protocol consists of a series of Purge , Move , Soak , Aspirate , Dispense and Fill operations. Purge , Move and Soak can be carried out in any sequence. Aspirate , Dispense and Fill can only be carried out within a Mo...

Wash Protocol Operations Operation Description Dispense Dispenses a specified amount of fluid into the wells after aspirating the contents of the wells (i.e. wherever a DISPENSE step is added to a protocol, the OPSYS MW will automatically aspirate the wells before dispensing more fluid in order to p...

Creating a New Wash Protocol Creating a New Wash Protocol Specifying the Header NOTE: Pressing CANCEL while creating or editing a protocol will bring you back to the main menu without saving your changes. or (or use keypad) Select one of the 40 protocol numbers. ↓ PROGRAM Select PROGRAM from the mai...

Creating a New Wash Protocol To create a new wash protocol header: 1. Display the main menu. 1: - - - - - - - - - - SETUP PROGRAM 2. Use the up or down scroll keys to display the protocol number (or use the keypad to enter the protocol number) that is to be created: 6: - - - - - - - - - - SETUP PROG...

Creating a New Wash Protocol Specifying Wash Cycle Operations The final step in creating a wash protocol is to specify individual operations within the wash protocol. An example of a wash protocol is shown in 45. In this example, the dispense wash pins are purged, and then a Dispense and Aspirate ar...

Creating a New Wash Protocol To specify a Move cycle: 1. Display the header information for the selected wash protocol, as outlined on page 41. The display will read: Select option MOVE PURGE 2. Select MOVE. The display will read: Start strip? 1 3. Using the keypad, enter the strip number where the ...

Creating a New Wash Protocol To specify a Dispense (within a Move): Note : A Dispense or Aspirate must be performed within a Move cycle. 1. Select DISPENSE. The display will read: Fluid volume? 350 2. Use the keypad to enter the volume of fluid to be dispensed. Note : A volume ranging from 50 to 999...

Creating a New Wash Protocol Ending a MOVE Cycle 1. Scroll right to display the next selection. The display will read: Select option SOAK FILL 2. Scroll right to display the next selection. The display will read: Select option END LOOP 3. Select END LOOP. The display will read: Select option MOVE PU...

Creating a New Wash Protocol To specify the End of the wash protocol: 1. Scroll right to display the next selection. The display will read: Select option SOAK END 2. Select END. The display will read: 1: NAME_01 SETUP PROGRAM Opsys MW™ User’s Guide 55

Modifying a Wash Protocol Modifying a Wash Protocol An existing wash protocol can be modified by using many of the same commands that are used to create a wash protocol. To modify a wash protocol: 1. Display the main menu: 1: NAME_01 SETUP PROGRAM 2. Use the up and down scroll keys to select the was...

Modifying a Wash Protocol To modify a wash operation: 1. Select EDIT while the operation is displayed. The first step in the operation is displayed: Start strip? 1 Cycles Note : The actual step that is displayed depends upon the operation being modified. 2. Modify the operation, using the same proce...

Modifying a Wash Protocol To delete a wash operation: 1. Select DELETE while the operation is displayed. The operation is removed from the wash protocol. Note : If a MOVE operation is deleted, all operations within the MOVE (i.e., Aspirate , Dispense , Soak and Fill ) are also removed. Opsys MW™ Use...

Modifying a Wash Protocol To insert a wash operation: 1. Scroll right while the operation that the new operation is to precede is displayed: MOVE 1-10 1 Cycles INSERT Note : The inserted operation will take place immediately before the operation that is displayed. 2. Select INSERT. The display will ...

Copying a Wash Protocol Copying a Wash Protocol An existing wash protocol can be copied to another program number. If a wash protocol is already in that location, it will be overwritten. To copy a wash protocol: 1. Display the main menu: 1: NAME_01 SETUP PROGRAM 2. Use the up and down scroll keys to...

Preparation Chapter 5 Washing a Plate Once the parameters for at least one plate type and one wash protocol have been set, the system can be used to wash a plate. Preparation The only preparation that is required is to check the fluid levels in the Wash Container and the Waste Container. Service the...

Preparation To refill the wash container: 1. Separate the dispense line quick-connector in the cap of the Wash Container. 2. Unscrew the cap of the Wash Container and lay it and the Pick- Up Filter on a clean paper towel. 3. Fill the Wash Container with the wash fluid that is being used. Note : If t...

Washing a Plate Washing a Plate A plate is washed by positioning it on the carrier block, selecting the wash protocol to be run, and pressing the START key. To wash a plate: 1. (If needed) Turn on the instrument. The display will read: 1: TESTNAME SETUP PROGRAM 2. Place the microplate on the plate c...

Routine Maintenance Procedures Chapter 6 Routine Maintenance Routine Maintenance Procedures The Opsys MW™ Microplate Washer is constructed from materials that resist chemical attack. If a spill occurs, it should be cleaned up as soon as possible using a cloth moistened with a mild laboratory deterge...

Routine Maintenance Procedures • Replace the dispense (Wash) tubing. • Replace the aspirate (Waste) tubing. Note : The dispense tubing and aspiration tubing may need to be replaced more frequently than every six months, depending upon the frequency of use and the severity of operating conditions. Co...

Cleaning and Decontamination Cleaning and Decontamination The Opsys MW™ is constructed from materials that resist chemical attack. Spills should be cleaned as soon as possible. If you need to decontaminate the Opsys MW™ instrument (for example, before servicing the instrument), clean the system and ...

Cleaning the Containers Cleaning the Containers Procedures for emptying, cleaning and refilling the Wash Container and the Waste Container are described below. To empty and clean the waste container: 1. Unscrew the cap of the Waste Container. Lay the cap and level sensor assembly on a paper towel. 2...

Replacing the Tubing Replacing the Tubing To replace the dispense tubing: 1. Separate the dispense line quick-connector in the cap of the Wash Container. Unscrew the cap, lay it and the Pick-Up Filter on a clean paper towel. 2. Discard the contents of the Wash Container. Thoroughly rinse the contain...

Replacing the Tubing To replace the vacuum tubing: Note : This procedure describes replacement of the vacuum tubing connecting the Waste Container to the Foam/Aerosol Trap. The vacuum tubing connecting the Foam/Aerosol Trap to the rear of the instrument should not require changing. 1. Unscrew the Ca...

Replacing the Tubing To replace the waste tubing: 1. Unscrew the Cap of the Waste Container and lay it with the level sensor assembly on a clean paper towel. 2. Discard the contents of the Waste Container in accordance with local regulations. Thoroughly rinse the container with deionized water and l...

Pump Calibration Pump Calibration Each Opsys MW™ washer manifold, or head, (e.g., 1 x 8, 1 x 12) has three calibration values stored in memory. These values are calculated and stored during manufacture at DYNEX Technologies. When the Opsys MW configuration is changed from one manifold to another, th...

Operational Problems Chapter 7 Troubleshooting Operational Problems Problems that may occur during normal operation of the Opsys MW™ are described below. For each problem, the symptom, probable cause and the means for resolving the problem are shown. Symptom Probable Cause Resolution The instrument ...

Service Procedures Chapter 8 Service Service Procedures The only user service procedures that can be carried out for the Opsys MW™ are listed below: • Removal and replacement of the wash head assembly • Cleaning of the wash head assembly To remove the wash head assembly: 1. Loosen the two thumbscrew...

Spare Parts Spare Parts Accessories 13001580 Plate Holder Assembly 13001610 8-Way Head Assembly 13001630 12-Way Head Assembly 352104000 Cleaning Wire, 0.040” 352101800 Cleaning Wire, 0.018” 91000051 User Manual 91000070 OPSYS MW Quick Set Up Instructions 23501800 Bottle Wide Mouth (2120-0010, Nalgen...

Returning a Washer for Service Returning a Washer for Service If the instrument must be returned for service, it must be cleaned and decontaminated if it has been in contact with potentially infectious body fluids (including human blood), pathological samples, or toxic or radioactive materials. Note...

Returning a Washer for Service EQUIPMENT IN TRANSIT IMPORTANT: Please include a copy of this form with each instrument. If your instrument contains a hard drive, please retain back-up copies of any stored files. Failure to do so may result in the loss of those files . Return Authorization Number: __...

Contact DYNEX Technologies Contact DYNEX Technologies DYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151 USA Tel: 703-631-7800 Toll Free: 800-288-2354 Fax: 703-803-1441 www.dynextechnologies.com Opsys MW™ User’s Guide 85

Index Index as required maintenance, 68 ASPIRATE, 39 aspiration height, 4 entering, 32 bottom wash specifying, 34 bottom wash height, 4 CAL. command, 36 calibration prototcol, 74 cleaning, 69 Wash Container, 70 Waste Container, 70 configuration, 28 connections, 17 daily maintenance, 67 data bits, 9 ...