Sanyo MCO-19M(UVH) - Manual

9 INCUBATOR COMPONENTS 18 Rear Right Side 21 Rear Left Side For MCO-19M(UVH)/19M(UV) or when MCO-19UVS is installed. 22 19 20 23 1 2 4 5 6 7 8 9 3 12 13 16, 17(inside) 15 24 14 10(inside) Handles Handles 25 11 For MCO-19M(UVH)/19M(UV) or when MCO-19UVS is installed.

12 Control panel and keypad 1. Upper limit regulator : This regulator is used to set the upper temperature limit. 2. OVERHEAT indicator : This indicator lights when the chamber temperature reaches the upper limit. 3. MENU Key : Press this key to access the menu. 4. Contrast knob : Turn this knob to ...

13 Remote alarm terminal The remote alarm terminal is located at the rear right side of the incubator. The alarm is outputted from this terminal. Contact capacity is DC 30 V, 2 A. Contact output: between COM. and N.O. between COM. and N.C. At normal Open Close At abnormal Close Open Note: The alarm ...

14 INSTALLATION SITE For correct operation of the Incubator, install it in a location with the following conditions. WARNING When using CO 2 gas for control, make sure that there is adequate ventilation . Using CO 2 gas in a small room without adequate ventilation may cause gas poisoning or oxygen d...

15 INSTALLATION 1. Remove the packing tape and clean up. Remove all the tape that is securing the doors and inner attachments. Open the doors for ventilation. If the outer panels are dirty, dampen a cloth with a diluted neutral detergent and wipe them. (Using an undiluted solution may damage the pla...

17 Connection of N 2 (or O 2 ) gas cylinder WARNING Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are connected correctly and are not liable to become disconnected. Ensure that the gas pressure is set at the specified value. Improper connection of the gas pip...

19 PREVENTING CONTAMINATION To prevent contamination of the chamber, select a suitable installation site. z Avoid locations with high temperatures or humidity. Avoid locations with high temperatures or humidity, because of a greater presence of microorganisms in the air. z Avoid locations with passe...

20 PRECAUTIONS FOR CULTURES z Leave space between culture containers. Always leave space for ventilation between culture containers (Petri dishes, flasks, etc.). Inadequate spacing may result in uneven temperature distribution and CO 2 gas concentration. z Do not place harmful materials in the chamb...

21 Using the Unlock Key z Unlocking when power is interrupted If power is interrupted to the MCO-19M(UVH)/19M(UV) with an MCO-HL installed, the outer door is electrically locked. To unlock the outer door while the power is interrupted, use the unlock key that is provided. To lock the outer door agai...

22 LCD PANEL     The following display (called the Top Display) will appear when the power switch is turned ON. The default temperature is 37.0 o C, the default CO 2 density is 0% and the default O 2 density is 20%. The date and time are preset at the factory. Refer to page 38 to change the date and...

24 BASIC OPERATIONS ON CONTROL PANEL     The following operation are possible through control panel:     z Setting the temperature ・・・ The chamber temperature can be set (page 25). z Setting the CO 2 density ・・・ The chamber CO 2 density can be set (page 25). z Setting the O 2 density ・・・ The chamber...

25 BASIC PARAMETERS       Setting the chamber temperature, CO 2 and O 2 density     The setting procedure for the chamber temperature, CO 2 and O 2 density are given below. (Default settings:   Chamber temperature: 37 o C, CO 2 density: 0%, O 2 density: 20%)   The Incubator automatically starts oper...

26       Setting the key lock     1. To set the key lock, change the value of the key lock parameter from 0 to 1 on the Stand-by Setting Display and press the ENTER Key. The buzzer will sound briefly and the keys will be locked.             2. “Key Lock” will be displayed in reverse video at the rig...

27       Releasing the key lock     1. To remove the key lock, change the value of the key lock parameter from 1 to 0 on the Stand-by Setting Display and press the ENTER Key.              2. The cursor will move to the Password Field. Input the 4-digit password (default: 0000) and press the ENTER Ke...

28       Setting the key lock password   1. From the Top Display, press the MENU Key to display the menu, select Tools, and press the ENTER Key.             2. Select Key Lock PW Setting from the Select Tools Display, press the MENU Key to display the menu, select OK , and press the ENTER Key.      ...

32   Setting the upper limit alarm temperature   An upper limit temperature alarm is provided with the Incubator. The alarm temperature can be changed by using the following procedure.   1. In the Stand-by Setting Display, turn the upper limit regulator on the control panel using a small screwdriver...

33 ALARM PARAMETERS     1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTER Key.             2. Select Alarm Setting from the Select Tools Display, press the MENU Key to display the menu, select OK , and press the ENTER Key.                 3. The Alarm ...

34 UV LAMP PARAMETERS   A UV lamp is used with the MCO-19M(UVH)/MCO-19M(UV) or the MCO-19M with an MCO-19UVS UV Lamp Expansion Kit installed. Use the following procedures to make the settings.       Using the UV lamp     A UV lamp is located inside the duct to sterilize the water in the humidifying ...

35       Precautions when using the UV lamp   WARNING Do not look directly at UV light. UV light is harmful to the eyes. z Always use the humidifying pan and humidifying pan cover. The humidifying pan and cover prevent UV light from escaping. Always use them even when not humidifying. To check wheth...

36   Setting the UV lamp ON period   Use the following procedure to change the setting of the UV lamp ON period.     1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTER Key.             2. Select UV Setting from the Select Tools Display, press the MENU K...

37   • The UV timer can be set from 0 to 30 min. The default setting is for 5 min. When the UV timer is set to zero, the UV lamp will not light. • If the outer door is opened while the UV lamp is lit, the lamp will turn OFF. Then, when the door is closed, the lamp will light for the preset period of...

38 OTHER PARAMETERS     Setting the date, time, and log interval   1. Press the MENU Key from the Top Display to display the menu, select Tools and press the ENTER Key.             2. Select Date Time from the Select Tools Display, press the MENU Key to display the menu, select OK , and press the EN...

41 DISPLAYING THE LOG     A log of the past chamber temperatures, CO 2 densities and O 2 densities can be displayed on a graph.   1. Press the MENU Key from the Top Display to display the menu, select Log and press the ENTER Key.             1 T e m p 3 7 . 0 o C C O 2 5 . 0 % O 2 5 . 0 % 2 3 4 5 S ...

43   Transferring data   Use the following procedure to transfer the log data to a PC.       1. To transfer the log data for one day, press the MENU Key to display the menu, select PC 1D, and press the ENTER Key. To transfer all of the log data, select PC All and press the ENTER Key.             2. ...

44 WATER LEVEL SENSOR This Incubator is equipped with a water level sensor for the humidifying pan. The sensor is set automatically when the humidifying pan is installed. Take care not to damage the sensor when removing or installing the humidifying pan. When the humidifying pan is removed (side vie...

45 ROUTINE MAINTENANCE Cleaning the chamber and inner attachments When using the Incubator for the first time or when performing H 2 O 2 decontamination, refer to Removing Chamber Attachments on the following page and remove and clean the inner attachments. WARNING Before performing any repairs or m...

48 Filling the humidifying pan Use the following procedure to fill the humidifying pan or to replace the water. 1. Lift the humidifying pan cover. (See Fig. 1.) 2. Pull the humidifying pan forward. (See Fig. 2.) 3. Dispose of the remaining water in the pan and clean the pan with a neutral dishwashin...

49 H 2 O 2 DECONTAMINATION   When the chamber has been contaminated, or when cleaning the chamber prior to starting a culture, H 2 O 2 decontamination can be performed using the H 2 O 2 Generator. z The following products must be purchased separately in order to perform H 2 O 2 decontamination. Befo...

51 H 2 O 2 decontamination Use the following procedure to perform H 2 O 2 decontamination using the H 2 O 2 Generator (MCO-HP). 1. Take out all trays, tray supports, side supports, the humidifying pan cover, the N 2 or O 2 gas injection nozzle, the N 2 or O 2 gas injection nozzle tube, the humidifyi...

52 6. Press the H 2 O 2 Key for 3 seconds. The system check will start. 7. If the system is normal, the following display will appear. (If the system is not normal, refer to Table 2 on page 57.) Select OK and press the ENTER Key to start H 2 O 2 decontamination. Decontamination will be performed aut...

54 12. Dilute the remaining H 2 O 2 reagent in the H 2 O 2 Generator with a large volume of water and dispose of it. Rinse and wash the H 2 O 2 Generator with distilled water. Then keep the H 2 O 2 Generator in a clean environment outside of the chamber. Note: After H 2 O 2 decontamination, cover th...

58   CALIBRATION Temperature/CO 2 /O 2 calibration 1. Press the MENU Key from the Top Display to display the menu, select Tools , and press the ENTER Key.             2. Select Temp/CO2/O2 Calibration from the Select Tools Display, press the MENU Key to display the menu, select OK , and press the EN...

60   TROUBLESHOOTING If the Incubator does not seem to be working properly, check the following items before calling for service. Symptom Items to check and countermeasures The Incubator does not operate at all. • Is the Incubator plugged in? • Is there a power outage, or has a circuit breaker inter...

62 DISPOSING OF THE MULTI-GAS INCUBATOR When disposing of the multi-gas incubator, contact a Sanyo sales representative or agent. WARNING The incubator must be dismantled and disposed of by qualified personnel only . If the incubator is left where outsiders enter, it may result unexpected accident (...

72 STACKING INCUBATORS Use the following procedure to stack Incubators. This work is potentially dangerous, so contact a Sanyo representative or agent. CAUTION • Select a floor that is strong enough to support the stacked incubators. • Never stack three or more incubators. Doing so is dangerous. 1. ...

73 Fig.A Stacking plate B Stacking plate A Protective sticker Front panel Hook Front

75 Name Multi-gas Incubator Models MCO-19M MCO-19M(UV) MCO-19M(UVH) Accessories 3 trays, 3 sets of tray supports, 3 gas tubes, 1 humidifying pan, 1 gas injection nozzle, 1 gas injection nozzle tube 1 pair of stacking plates A and B, 2 protective stickers, 6 tube bands Optional accessories H 2 O 2 Ge...

76 CAUTION Please fill in this form before servicing. Hand over this form to the service engineer to keep for his and your safety. Safety check sheet 1. Unit contents Risk of infection: Risk of toxicity: Risk from radioactive sources: (List all potentially hazardous materials that have been stored i...