Roper 4411-0046 - Manual

Roper 4411-0046

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Table of Contents:

  • Page 3 – iii; Table of Contents
  • Page 4 – iv
  • Page 8 – viii
  • Page 10 – Figures
  • Page 12 – xii
  • Page 14 – xiv
  • Page 16 – xvi
  • Page 18 – xviii; This page intentionally left blank.
  • Page 21 – Part 1; Getting Started; , is primarily intended for the first time user or for the; Part 2; Advanced Topics; Part 3; Reference; , contains a chapter having alphabetically arranged detailed; Summary of Chapter Information; Part 1, Getting Started; Introduction; lists system requirements and describes how to
  • Page 22 – describes the data file display options.; Part 2, Advanced Topics
  • Page 23 – Online Help
  • Page 24 – These TXT files can be opened with any ASCII text editor.
  • Page 25 – If your computer and system were purchased together from Roper; System Requirements; Hardware requirements; Princeton Instruments (PI) ST series controller and a PI camera.
  • Page 26 – Operating System requirements; Installing WinView/32; Following are the steps to install WinView/32 on your computer.; Installation from CD; (where “x” is the letter designating your CD; Enter; key on your keyboard. The install sequence will begin.
  • Page 27 – Steps Common to Installations from CD and FTP Site; Log onto the FTP site.
  • Page 28 – shown in Figure 7. Initially the; Next; button will be grayed out. When the correct
  • Page 32 – in the Create Directory dialog box or; OK; in the
  • Page 36 – Yes
  • Page 37 – Finish; completes the installation.; Default Directories; compatibility, filenames have been kept short.
  • Page 38 – Aborting the WinView/32 Installation; Cancel; Installing Some WinView/32 Files at a Later Time
  • Page 39 – Uninstalling and Reinstalling
  • Page 44 – Cursor; Title Bar
  • Page 45 – Control Menu; command is unavailable if the window is MAXimized.; Window Control Boxes; Menu Bar
  • Page 46 – selection; Tools; selection and listed Snap-Ins and Add-Ons; Custom Toolbar...; on; Toolbars; on the View Menu opens the Toolbar Selection menu (Figure 36).
  • Page 47 – Right-Click menus Accessed from Open window
  • Page 49 – Customizing the Custom Toolbar; Dialog Boxes
  • Page 50 – Data Entry & Parameter Selection Devices; Edit Boxes and Text Boxes:
  • Page 51 – List Boxes and List Views:
  • Page 52 – . Each time you click on
  • Page 53 – Data File Display
  • Page 55 – Controller Selection
  • Page 56 – Not active on first wizard screen.; Advance to second wizard screen.
  • Page 57 – Interface Selection; Returns you to the previous wizard screen.; Closes the wizard previous selections remain in effect.
  • Page 58 – Controller/Detector page; Select MicroMAX
  • Page 59 – SCAN; Logic Out; Display page
  • Page 60 – MHz MicroMAX; None
  • Page 62 – Select MicroView; Full Frame; is the only available readout mode.; See Display Page discussion on page 82.
  • Page 63 – Interface page
  • Page 64 – Controller page; Shutter Compensation Time:
  • Page 66 – Select PentaMAX
  • Page 74 – Set to match the version of your ST-133. Later versions of the ST-
  • Page 76 – Select the camera type installed in your system.; The selections are Full Frame, Frame Transfer and Kinetics. Frame
  • Page 78 – Video Focus; V/ICCD manual for additional information.; External Trigger
  • Page 80 – User Defined Chip
  • Page 81 – User Defined Timing
  • Page 82 – Display Tab Page; Image Orientation
  • Page 83 – Interface Tab Page; Select if PI ISA high-speed serial Interface card is installed
  • Page 85 – Start Storage; is selected. This mode is particularly convenient
  • Page 86 – Temperature Control; to the desired value (0°; LED on the panel of the Analog/Control module; dialog
  • Page 87 – Experiment Setup Procedure (all controllers); Main; WARNING
  • Page 89 – ADC; For systems having more than one A/D converter, both FAST and SLOW
  • Page 92 – Data Collection Procedures (controller specific); Focusing
  • Page 95 – Data Collection
  • Page 97 – Data Collection - All Controllers
  • Page 99 – Chapter 5
  • Page 101 – Open
  • Page 102 – Saving Data Files; The Save As dialog box is used to save the active data file to a user-; Saving temporary data files; Indicates the file type. The data types currently supported are
  • Page 103 – Data File tab page; Closing a data file
  • Page 104 – The; Close; in the Window menu closes all open data files.; Deleting Data Files
  • Page 105 – Delete; to delete all of the selected files.
  • Page 107 – Image; and; D Graph; . If you wish to control the
  • Page 108 – Displaying circuit.spe; If the
  • Page 110 – Selecting a region of interest; Click on the
  • Page 111 – Opening the Display Layout window
  • Page 112 – Viewing axes and cross sections; Range
  • Page 113 – The information box
  • Page 114 – Relabeling the axes; Pixel; Strip
  • Page 115 – Changing the Color of the Axes and Labels; Axis; button. The color palette will open as shown in Figure 98.
  • Page 116 – Specifying a new ROI and intensity range; Data Displayed as a 3D Graph
  • Page 122 – When you open the information box, it becomes the active window. To; Displaying a single strip; On the General tab page, with Auto Select unselected, click on the; Display As; radio button and then on; . The single data curve for the strip on which
  • Page 123 – Small
  • Page 125 – Hidden surfaces
  • Page 129 – Settings
  • Page 130 – Save Label; box to save the changes.
  • Page 131 – Screen Refresh Rate
  • Page 135 – Overview; click on the; Use Full Chip; radio button on the Experiment Setup Main tab page. As; Defining a single region
  • Page 136 – button on the Main tab page.
  • Page 137 – Definition of multiple regions
  • Page 139 – Storing the access pattern
  • Page 141 – Acquiring background files; This operation is similar to normal data acquisition.; Acquire Background; from the Acquisition menu. This will immediately
  • Page 143 – Flatfield Correction; Acquiring a flatfield file; Acquire Flatfield; Automatic flatfield correction
  • Page 145 – Operation
  • Page 146 – File tab pages; Parameters
  • Page 147 – Operation Descriptions; Linear Operations
  • Page 148 – Non-Linear Operations; The natural log of the; Bitwise Operations; Decimal
  • Page 149 – Binary Operations; Procedure
  • Page 151 – Edge Enhancement; Input tab page; parameters will; Mouse; button is only active when the; Input; tab page is selected.
  • Page 152 – Parameters tab page; A mask is an n
  • Page 153 – Laplacian masks; The replacement for the center cell, called R, is
  • Page 155 – mask, select User Defined and change the Kernal; Sharpening Functions
  • Page 157 – Sharpening Procedure
  • Page 158 – Smoothing Functions; button at the bottom of the window allows you to use the
  • Page 159 – Post-Acquisition Processing Mask Operations
  • Page 160 – Smoothing Procedure
  • Page 161 – Morphological Functions
  • Page 165 – Filter Matrix tab page; Load Defaults; button sets the matrix parameters to their factory-; Output tab page
  • Page 166 – Custom Filter Procedure
  • Page 167 – Look Up Table; Look-Up Table tab page
  • Page 168 – References
  • Page 169 – Threshold and Clipping
  • Page 170 – Cross Section
  • Page 172 – Binning and Skipping
  • Page 173 – Restrictions and limitations
  • Page 174 – Histogram Calculation
  • Page 177 – Printing
  • Page 178 – Click on OK to initiate the print.; Print Preview; Opens the Print screen so that a print can be initiated.
  • Page 179 – Printing a Screen Capture
  • Page 180 – Saving as TIF and Printing
  • Page 181 – Pulser Operation; Pulsers; on the; Setup; menu and; PG-200 Programmable Pulse Generator
  • Page 182 – Comm Port; tab to bring the Comm Port tab page to the front (Figure
  • Page 184 – selection should be
  • Page 186 – menu select; to open the Pulsers dialog box.
  • Page 187 – PTG; 66) will open. If PTG is grayed out on the; dialog box, PTG support has; tab to bring the Comm Port tab page (Figure 167) to the
  • Page 195 – to close the window.
  • Page 197 – Custom Toolbar Settings; Custom Toolbar button descriptions; on page 239 for information about the; Customizing the Toolbar
  • Page 198 – Adding an Available Button; Add; Removing a Button; Remove; Individual Dialog Item Descriptions
  • Page 199 – Move Down; determines the position of a given; Move Up; determines the position of a
  • Page 203 – Menus and Dialog Boxes; .SPE to ASCII Conversion dialog box; When frames are converted and the output option is; will result in output names; One; is selected, the output filename will not include a number.; Parameters and Buttons:; Displays the name of the file to be converted.
  • Page 204 – D Layout tab page
  • Page 206 – Checking the Marker Curves On box will cause every nth curve,
  • Page 207 – Save as Default check box:; Saves settings on all Display Layout tab pages. These
  • Page 208 – A page; file page. A description of the items on this; The datatype of the
  • Page 209 – Acquisition menu
  • Page 211 – White Clamping Level; Black Clamping Level; text boxes or by using the associated spin buttons.
  • Page 212 – Opens context-sensitive help for whichever tab page is on top.
  • Page 214 – Area Under Curve dialog box
  • Page 215 – Aux Trig Out tab page; PTG Aux Trig Out; Axes; Fonts
  • Page 217 – Axes display layout tab page
  • Page 218 – Saves settings on all pages. These settings will be; B page
  • Page 220 – Brightness/Contrast dialog box
  • Page 222 – C page
  • Page 223 – Camera State dialog box
  • Page 224 – Cleans/Skips tab page; Cleans; For most applications the default value should be used.; Number of Strips per Clean:; Sets the number of CCD data strips to be
  • Page 225 – Vertical Skips; Sets the size, in strips, of the skip blocks that immediately; Color display layout tab page
  • Page 227 – Saves settings of all Display Layout tab pages.; Palette Dialog Box Buttons:
  • Page 228 – Continuous Pulsing Setup dialog box; Closes the dialog box leaving the original settings in effect.
  • Page 229 – Control Box Focus Window; Selecting; on the Acquisition Setup menu with V/ICCD as the selected
  • Page 230 – Control menu; Maximize
  • Page 231 – Click the maximize icon on the Title bar or double-click; Controller/Camera tab page
  • Page 233 – Following parameters only apply with V/ICCD or V/ICCD Control Box
  • Page 234 – Controller Registers dialog box; See discussion of the; Diagnostics: Controller Registers dialog box; The min and max values for each cross section profile are
  • Page 236 – Cursor tab page
  • Page 237 – Saves settings of all Display Layout tab pages. These; Cursors
  • Page 238 – Custom chip tab page
  • Page 239 – Toggles between a 256 grayscale display and a 256
  • Page 240 – Color Bar is not; Info; logarithmic. Equivalent to selecting or deselecting; Log; Views ten exposures earlier. If the current exposure
  • Page 241 – This button acquires a flatfield file. It is equivalent to
  • Page 242 – Intensifier Gain; Acts as spacer between buttons, allowing them to be arranged in; Customize Toolbar dialog box
  • Page 244 – Focus; or; Acquire; Enter a template name for the data you are about to collect. Use
  • Page 247 – Data window; If; Colorbar; has been
  • Page 250 – Clicking this button attempts to set the zoom factor to 1:1.; Data Window Context menu
  • Page 252 – Opens the Display Layout dialog box.; Detector Temperature dialog box
  • Page 253 – DG-535 Comm Port tab page; Clicking on this button immediately sends all current dialog; Click on this button to open the online help for this window.
  • Page 254 – DG-535 Continuous Pulsing Setup dialog box
  • Page 255 – DG-535 Interactive Pulse Width and Delay dialog box; DG-535 Interactive Trigger Setup dialog box
  • Page 256 – DG-535 Pulsing tab page
  • Page 257 – Selecting ON activates the MCP bracket
  • Page 258 – DG-535 Pulse Sequence Setup dialog box; Fixed; Increment type). Linear sequential operation is well suited; With a Software Accumulations setting of one, Number of Images
  • Page 260 – Fast Decay; In a two species system, enter the decay time of the faster of the; Slow Decay; In a two species system, enter the decay time of the slower of the
  • Page 262 – DG-535 Triggers tab page
  • Page 264 – Diagnostics: Controller Registers
  • Page 265 – Diagnostics menu; For Additional Information; Controller Registers dialog box:; Do not disturb except with factory guidance.
  • Page 267 – Display menu; Menu Selections; specifying how the data is to be displayed.
  • Page 269 – Display tab page; Reverse is the only choice with an ST-121 Controller.
  • Page 271 – Don’t control screen saver
  • Page 272 – Execute dialog box; Execute Macro; Experiment setup window; Experiment Setup
  • Page 275 – Factory Defaults dialog boxes; This menu (Figure 239) provides the basic file functions as follows.
  • Page 277 – Font dialog box
  • Page 278 – General Display Layout tab page; When a data file is opened, it can be displayed as an image or as a
  • Page 280 – Get/Set Parameters dialog box; See; Diagnostics: Get/Set Parameters dialog box; discussion on page 265.
  • Page 281 – Closes the dialog box without executing any changes. Clicking on; Hardware Setup window
  • Page 282 – Help menu
  • Page 283 – Image Layout tab page
  • Page 284 – Zoom Factor Variable and Aspect Ratio Variable:; Image size changes if window; Brightness and Contrast; Image Orientation dialog box
  • Page 286 – Information box; is selected on the; View; menu and data is displayed, an information box for the
  • Page 288 – If the controller is a MicroMAX or ST-133, clicking on; from the; The Exposure Time is the amount of time the shutter will open to; RS170 Intensity Scaling:; Together with the Offset, the Scaling determines how the
  • Page 289 – Scaling
  • Page 291 – from the Acquisition menu; Determines how video will be mapped to a video monitor display.
  • Page 292 – Interactive Trigger Setup
  • Page 293 – Label dialog box
  • Page 294 – Load Factory Defaults dialog box; Load Factory Defaults; Load Factory Defaults from NVRAM dialog box
  • Page 295 – Macro menu
  • Page 296 – Use Region of Interest:; Data will be collected from region(s) defined on ROI Setup
  • Page 298 – Manual Scan Patterns
  • Page 299 – Math dialog box
  • Page 300 – t button on the custom toolbar, allows
  • Page 301 – Online Threshold Setup
  • Page 302 – Open dialog box; Features and Selections; Moves you higher in the directory tree.
  • Page 304 – Operation tab page; See Chapter 9 for additional information.
  • Page 306 – Options menu
  • Page 308 – Parameters tab page (Binning and Skipping)
  • Page 315 – Parameters tab page (Threshold and Clipping)
  • Page 316 – PG-200 Comm Port tab page
  • Page 317 – PG-200 Continuous Pulsing Setup dialog box
  • Page 318 – PG-200 Pulsing tab page
  • Page 319 – PG-200 Pulse Sequence Setup dialog box
  • Page 323 – Software Accumulations:; Sets the number of exposures to be taken for each Shot.; PG-200 Triggers tab page
  • Page 326 – PI Shell Extension
  • Page 327 – Print Preview screen; Selections
  • Page 328 – Print Progress box
  • Page 329 – Process menu
  • Page 330 – Binning and Skipping:
  • Page 331 – Processes tab page; Thresholding; , data that falls below; Photon Estimation
  • Page 332 – Properties window; was; was not installed, only the standard page is provided.
  • Page 333 – PTG Aux Trig Out tab page
  • Page 334 – PTG Comm Port tab page
  • Page 335 – PTG Continuous Pulsing Setup dialog box; PTG Interactive Pulse Width and Delay dialog box; button of the; The pulse width is the duration of the pulse applied to the PI-MAX
  • Page 336 – PTG Interactive Trigger Setup dialog box
  • Page 337 – PTG Pulse Counter; Pulse Counter; PTG Pulsing tab page; detailed information.
  • Page 339 – PTG Pulse Sequence Setup dialog box
  • Page 343 – PTG Triggers tab page
  • Page 344 – PTG window
  • Page 346 – Pulsing tab page; PTG Pulsing tab page:; Range Display Layout tab page; Allows you to turn; Initial Autoscaling; On or Off. When On, on opening a file but; Live Auto All Scans
  • Page 347 – . Checking Save as Default after deselecting
  • Page 348 – Readout Time box
  • Page 349 – Record Macro dialog box; allows user labels to be placed on the display.; ROI Setup tab page
  • Page 350 – Multiple readout patterns can be defined. The Starting Pixel,
  • Page 352 – Save As dialog box
  • Page 353 – Right Click Shortcut Menus; Save Factory Defaults
  • Page 354 – Save/Load Setup tab page; Clicking the; Save; button allows you to save to disk all of the Experiment Setup; Load; allows you to load a previously saved setup file. Because a
  • Page 355 – Screen Saver Configuration dialog box
  • Page 357 – Blemish Removal Pattern:
  • Page 358 – Setup Wizard
  • Page 361 – Split Frame dialog box; Standard toolbar buttons:; releases of the software.
  • Page 362 – List Help Topics. Equivalent to selecting Search for Help On; Context Sensitive Help:; Note that this is a two-step operation. The first; Statistics dialog box
  • Page 365 – Status bar
  • Page 366 – See Processes tab page discussion on page 331.; Timing Generator Interactive Pulse Width and Delay dialog box
  • Page 367 – Timing Generator Interactive Trigger Setup dialog box
  • Page 368 – Timing tab page; Applies only to the special Double Image; tab page sets the exposure
  • Page 371 – Tools Menu; Zap to Grams
  • Page 372 – Triggers tab page; PTG Triggers tab page:; TTL Diagnostics dialog box; TTL In
  • Page 373 – TTL Out; Closes the dialog box.
  • Page 374 – on the Acquisition; from the Main tab page of the Experiment Setup window.; Automatic Brightness:
  • Page 375 – V/ICCD Control Box Focus Window
  • Page 376 – These parameters allow you to independently
  • Page 377 – View menu; Window Toolbar is selected from the; Determines whether the Status bar at the bottom of the WinView/32
  • Page 378 – Virtual Chip dialog box; Clicking; Virtual Chip; on the Setup menu opens the Virtual Chip dialog box as shown
  • Page 381 – Window menu; If a data file is open and active, selecting New Graph, New Image
  • Page 382 – If images have been reduced to icons by minimizing them, selecting
  • Page 384 – This selection closes all open data windows.; selects the next file in the list to be active; Previous; selects the preceding file to be active. For example, suppose
  • Page 385 – WinX Data Player dialog box; while a playback is in
  • Page 388 – Header Structure Listing
  • Page 391 – Calibration Structures; There are three structures for the calibrations; Start of X Calibration Structure
  • Page 392 – WINX Header Structure (with actual offsets)
  • Page 396 – Start of Data; The data follows the header beginning at offset 4100.
  • Page 399 – Appendix B
  • Page 401 – Index
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4411-0046

Version 2.4.M

September 20, 1999

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Summary

Page 3 - iii; Table of Contents

iii Table of Contents Part 1 Getting Started................................................................ 19 Introduction ........................................................................................................ 21 Summary of Chapter Information .......................................

Page 4 - iv

iv WinView/32 Manual Version 2.4.M ST-133............................................................................................................................... 74ST-138..............................................................................................................................

Page 8 - viii

viii WinView/32 Manual Version 2.4.M Operation tab page .......................................................................................................... 304Options menu .................................................................................................................. 306Out...

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