Mitsubishi P91DW(UB)- Manuals

ImageQuant 300 Capture User Manual 1 Chapter 1: Introduction and Setup 1.1 ImageQuant Capture Imaging System The ImageQuant Imaging System is a powerful digital imaging system, ideal for instant photography of a wide variety of samples. The CCD camera allows imaging of low-light samples in UV-illumi...

ImageQuant 300 Capture User Manual 2 1.1.1 Mouse Functions The mouse supplied with the ImageQuant System has two buttons. The left button is used to activate functions and otherwise make selections when using the software. In some cases, the right mouse button is used to recall or reactivate the fun...

ImageQuant 300 Capture User Manual 3 1.2 ImageQuant Imaging System Setup 1.2.1 System Components The ImageQuant 300 System includes: • High-performance CCD camera • Zoom lens, close-up 2+ diopter lens, and interference filter • (optional) Computer with keyboard, mouse, and monitor • (optional) Windo...

ImageQuant 300 Capture User Manual 4 1.3 Computer, Camera and Peripheral Installation All software, peripheral drivers and operating systems have been pre-installed at the factory. During the system installation all components should only need to be plugged into the correct ports 1.3.1 Setting up th...

ImageQuant 300 Capture User Manual 6 1.3.3 Software Driver Installation Instructions This set of instructions only applies if you did not purchase an integrated system and need to install the firewire card (If the computer contains a pre-installed firewire card or an on-board firewire port, please s...

ImageQuant 300 Capture User Manual 7 7. Open Device Manager and find the exclamation mark under Imaging Devices. Right- click on Generic 1394 Desktop and select Update Driver : 8. Select No, not this time when you are asked if you want to connect to Windows Update to search for a driver:

ImageQuant 300 Capture User Manual 8 9. Select Install from a list or specific location (Advanced) : 10. Select AlphaImager Camera :

ImageQuant 300 Capture User Manual 9 11. Select Continue Anyway if a digital signature message appears. 12. After the driver installation is complete, the error message should be resolved in the device manager. 13. Exit out of the device manager.

ImageQuant 300 Capture User Manual 10 1.3.4 Camera Installation The ImageQuant camera connects via a standard IEEE 1394 firewire connection. If the system was shipped with a computer, the firewire card will be pre-installed. If the system was not shipped with a computer it will be necessary to insta...

ImageQuant 300 Capture User Manual 12 1.3.6 Cabinet Assembly (optional) When you remove the light cabinet from its shipping carton, it is already partially assembled. The camera mounting assembly is packed separately in the same container. The UV transilluminator and cabinet top are both packed in s...

ImageQuant 300 Capture User Manual 13 Installing the UV Transilluminator Take the following steps to install the UV transilluminator (this is a box-shaped UV lighting apparatus that rests at the bottom of the cabinet): 1. At the bottom of the cabinet, there is an extendable metal tray that is held i...

ImageQuant 300 Capture User Manual 14 Unfastening the Fold-Down White Light Tray The cabinet contains a fold-down light tray that is locked for shipping (see illustration below). Take the following steps to unfasten the light tray: 1. The light tray is held in place by a detachable knob that screws ...

ImageQuant 300 Capture User Manual 15 Attaching the Hood Take the following steps to attach the cabinet hood and connect the hood fan: 1. Place the hood on top of the cabinet so the holes for screws are centered over the corresponding holes in the top of the cabinet. 2. From within the cabinet, atta...

ImageQuant 300 Capture User Manual 17 1.4 ImageQuant System Quick Guide Note: This is intended as a quick reference guide for acquisition. For more detailed information on the individual features reference section 1.6 of this manual. 1. Power on the system: a. Turn on the computer, monitor, and opti...

ImageQuant 300 Capture User Manual 19 1.4.1 Cabinet Controls (for optional cabinet) Transillumination Controls These two buttons are used to toggle the UV transilluminator and the reflective white light table on and off. Reflective Light Controls These two buttons are used to toggle the reflective U...

ImageQuant 300 Capture User Manual 20 1.5 System Information To display system information, select the About option in the Help menu. This button accesses a pop-up box. ABOUT Pop-Up Dialog This box shows the instrument serial number (where appropriate) and the Software version number. Use the inform...

ImageQuant 300 Capture User Manual 21 1.6 Acquiring an Image In the TOOL BAR window, click on the Camera Acquire icon: Once the Camera Acquire Icon is pressed, the CAMERA SETUP AND REVIEW window is activated to provide exposure control of the camera, sensitivity/resolution controls, all lighting and...

ImageQuant 300 Capture User Manual 23 1.6.2 Control of the Optional Motorized Zoom Optics ImageQuant Capture controls the motorized optics when present. The software allows you to set the Aperture, Focus and Zoom (if applicable) from the software acquisition window. To change a value for the apertur...

ImageQuant 300 Capture User Manual 24 1.6.3 Auto Exposure and Auto Exposure Setup ImageQuant Capture’s acquisition window includes a check box and setup button for Auto Exposure. Auto Exposure Setup (Auto Exposure Compensation Setting): 4 choices available • Normal Exposure for image saturation: Use...

ImageQuant 300 Capture User Manual 26 1.6.4 Cabinet Controls - Activating the Light Source and Selecting a Filter Use the mouse to click on the desired light source in the ‘virtual’ cabinet control interface or you can use the controls on the cabinet itself. Both mechanical and software controls are...

ImageQuant 300 Capture User Manual 27 1.6.5 Use Preview to Set Exposure Time Once the sample has been positioned and the camera has been focused, close the door to the ImageQuant light cabinet and make sure that the appropriate illumination source is turned on. Also make sure the cabinet door indica...

ImageQuant 300 Capture User Manual 28 1.6.6 Optimizing the Gray Scale for Saturation and Contrast Displays If an image will be analyzed, it is important that it not be over-saturated (too light) or under-exposed (too dark). Using Show Saturation , the user can see the areas of the image that are ass...

ImageQuant 300 Capture User Manual 29 1.6.7 Acquire and Transfer the Image Once the appropriate image is displayed on the screen, click on the CAPTURE button to capture and display the image in ImageQuant Capture. The acquired image will be automatically adjusted by a zoom factor based on the resolu...

ImageQuant 300 Capture User Manual 30 1.6.8 Capturing Images Using the FrameGrabber Mode If kinetic, multiplex, color, or chemiluminescence experiments are desired where you wish to have the system automatically capture several images at preset exposure times, preset time delay between images, prese...

ImageQuant 300 Capture User Manual 32 Once all images have been captured, the FrameGrabber tab will become displayed in the Tool Box. The buttons on this tab perform the following tasks: REC Opens the Camera Setup and Preview window to record a sequence. PLAY Display a continuous loop of all of the ...

ImageQuant 300 Capture User Manual 33 1.6.11 FrameGrabber: Save/Load FrameGrabber Setup Routines Two buttons “Save Setup” and “Load Setup” allow you to save and load all FrameGrabber setup parameters. Files are saved as *.mvf files. 1.6.12 Frame Stacking At the top of the Camera Setup and Preview wi...

ImageQuant 300 Capture User Manual 34 1.6.13 Capturing a Color Image Using the FrameGrabber Function A color image can be generated by acquiring three images each taken with a red, green and blue emission filter. Once saved, these images are then combined in the Overlay pull down menu. Open the imag...

ImageQuant 300 Capture User Manual 35 Chapter 2: Getting Started - Basic Imaging Functions 2.1 Contrast Adjustment The Contrast Adjustment window allows for the best visualization possible of a sample utilizing the black , white , and gamma adjustments, as well as, image reverse and auto contrast. T...

ImageQuant 300 Capture User Manual 36 2.1.1 Using the Contrast Adjustment Tools for Grayscale Images There are three sliding scales found in the image control area to the right of the image. Below each scale is a box displaying a number that corresponds to the position of the slider. By adjusting th...

ImageQuant 300 Capture User Manual 37 Black Level set at 0 Black Level set at 60 2.1.3 White Level Adjustment The number beneath the White Level scale also corresponds to a gray scale value. When the slider is at the very bottom of the scale, this number is 255. As the slider is moved upwards along ...

ImageQuant 300 Capture User Manual 39 2.1.6 The Reverse Button The Reverse button inverts the gray levels of the displayed image, converting a positive image to negative, or vice versa. For instance, an image with black bands on a white background is converted into an image with white bands on a bla...

ImageQuant 300 Capture User Manual 42 2.1.9 Using the Contrast Adjustment Tools for Color Images ImageQuant Capture can also import RGB color images. ImageQuant Capture automatically detects this process and the Contrast Adjustment tools are configured for color image adjustments. Contrast Adjustmen...

ImageQuant 300 Capture User Manual 43 2.2 Tool Bar The Tool Bar window provides intuitive buttons for the most common functions in ImageQuant Capture. Tool Bar The Open button functions identically to the File Open function in the upper menu bar. This function is used to open previously saved images...

ImageQuant 300 Capture User Manual 44 occurs. Saturation is a feature that is most important during the acquisition stages and is thoroughly detailed in the acquisition features of the system manuals. The Img Drag button is useful for to pan with a zoomed image. To activate this function, click on t...

ImageQuant 300 Capture User Manual 45 Clear removes any overlays currently displayed on the image. This function can be useful if annotations or other displays obscure parts of the image. Once an image is displayed, it can be printed on the default printer by clicking the Print button in the Tool Ba...

ImageQuant 300 Capture User Manual 48 3.1.1 File|Open This function opens an image that has been previously saved as a TIF, GLP, BMP, PCX, TGA, PIC, JPG or Macintosh ® TIFF (MAC) file. File Open Dialog Box Using the left mouse button, click on the name of the file to be loaded. That name is then hig...

ImageQuant 300 Capture User Manual 49 3.1.2 File|Overlay To superimpose images, use the OVERLAY function under the File menu. This function will display separate multiplexed images or a RGB color image as a compiled image with the appropriate color channel images added together. A simple way to acqu...

ImageQuant 300 Capture User Manual 50 3.1.4 File|Save and Save As Save allows original images to be saved in several different formats. Save As allows images that have previously been saved to be saved in a different location or as a different file type without affecting the original image. ImageQua...

ImageQuant 300 Capture User Manual 51 File Types TIFF is the default file format for ImageQuant Capture files. TIFF is an acronym for "tagged image file format" and was developed as a flexible and machine-independent graphic file format. Saving as a TIFF file will allow users to double-click...

ImageQuant 300 Capture User Manual 52 3.1.5 Printer Setup This function displays a dialog box in which the settings for the parallel printer are specified. When all the pertinent printing preferences have been specified, click on the OK button. If you purchased a printer with ImageQuant, this will b...

ImageQuant 300 Capture User Manual 53 3.1.6 Print This function sends the image to the default printer specified in Print Setup. 3.1.7 Logoff This function logs the current user out of ImageQuant Capture when security features are in use. 3.1.8 The Exit Function The Exit function closes ImageQuant C...

ImageQuant 300 Capture User Manual 54 3.2 The Edit Menu The Edit menu provides the ability to copy, crop and remove any annotations or filters that have been added to the original image. Edit pull-down menu To activate the Copy and Crop functionality, place a check mark next to EDIT ACTIVATION. This...

ImageQuant 300 Capture User Manual 55 Once this is completed, you can select either the COPY or CROP function in the EDIT menu options. COPY will copy the desired area of interest into the Windows Clipboard and allow you to paste into any desktop publishing package (i.e. Word, Excel, Adobe Photoshop...

ImageQuant 300 Capture User Manual 56 3.3 The Image Menu The Image menu option provides the ability to perform a variety of image processing functions. Image pull-down menu 3.3.1 Equalize The equalize option performs a duplicate function to the EQUAL option in the Contrast Adjustment Window. This is...

ImageQuant 300 Capture User Manual 57 most common application for quotient is for those technical users who run their own flat field corrections. This can be done using the Flat Field Calibrate selection under the Image pull-down menu which will be described in detail later in this section. All thre...

ImageQuant 300 Capture User Manual 60 3.4 The Setup Menu This menu customizes the system settings by allowing users to save default parameter preferences and customize the software settings. Setup pull-down menu 3.4.1 Default Parameters Default parameter files eliminate the need to readjust the syst...

ImageQuant 300 Capture User Manual 61 3.4.2 Save Defaults Every time a default file is saved, all of the settings for the parameters listed above are saved. These can be loaded later and applied to any image. Save Defaults Dialog Box Once any of the system settings have been changed, a new default f...

ImageQuant 300 Capture User Manual 62 3.4.4 Security When the system is shared by a number of people or laboratories, a method of keeping track of its use may be helpful. The Security feature allows various levels of security and user log functions. We suggest one user be designated as the superviso...

ImageQuant 300 Capture User Manual 63 3.4.6 Print Mode ImageQuant Capture provides custom printing options. Setup Print Image Info Dialog Box Printing can be achieved using three different methods: Full Image: Prints the original image. Does not print zoomed images or images overlaid with data scree...

ImageQuant 300 Capture User Manual 64 There are four tabs in the Preferences menu (ImageQuant Capture standalone software does not contain the Image Acquire or Cabinet Settings tabs): 1. General – Configure prompts and file saving/opening formats. 2. Image Acquire – Inverts the image seen by the cam...

ImageQuant 300 Capture User Manual 65 3. Cabinet Settings – Used for adjusting the port settings and customizing filter positions on the cabinet. 4. Auto Enhancements – Used to customize the Auto Enhance Levels located in the tool box. To make changes to the preferences, select your change by typing...

ImageQuant 300 Capture User Manual 66 3.5 The Overlay Menu The Overlay menu provides a means of saving and retrieving annotation overlays. This is especially useful when a standard gel format is run repeatedly. Lane numbers, molecular weight marker sizes, and other pertinent information can be store...

ImageQuant 300 Capture User Manual 67 The current directory is the one in which the new overlay file will be saved. If necessary, change the directory or drive as described in Section 3.1. Once a name has been entered and the appropriate directory has been accessed, click the SAVE button to save the...

ImageQuant 300 Capture User Manual 68 3.6 The Utilities Menu A number of functions are now handled by Windows programs. To access many of these programs while in ImageQuant Capture, open the Utilities menu and select the program of choice. Utilities pull-down menu 3.6.1 Explorer This option can be u...

ImageQuant 300 Capture User Manual 70 These tools are automatically displayed when ImageQuant Capture is launched for additional ease of use and to generate a common ‘look and feel’. However, if you would like to hide any of these tools they can be turned off in the View menu by just deactivating th...

ImageQuant 300 Capture User Manual 71 3.8 The Help Menu Help pull-down menu 3.8.1 On-Line Note On-line help is available from the ON-LINE NOTE section of the help menu. 3.8.2 About To display system information, select the About option in the Help menu. This button accesses a pop-up box. This box sh...

ImageQuant 300 Capture User Manual 72 Chapter 4: The Tool Box Image enhancement tools are contained within the Tool Box as indicated. This tool set allows the user to zoom the image, rotate-flip the image, show the image histogram, perform automatic image enhancement, annotate on the image, display ...

ImageQuant 300 Capture User Manual 74 4.3 Histogram The histogram is a graphical display of the proportion of pixels assigned to each of the image’s gray levels. This tool is found in the Tool Box . Histogram display in the Tool Box The image is made up of picture elements (pixels) having brightness...

ImageQuant 300 Capture User Manual 76 4.4 Automatic Enhancement The Enhance tool is found in Tool Box. This function is ideal for new or inexperienced users of the system since it offers 9 levels of automatic image enhancement of the black, white, and gamma levels simultaneously. For an inexperience...

ImageQuant 300 Capture User Manual 77 4.5 Annotations The annotation tools, found in the Tool Box, include a number of different options for adding text (including Greek symbols), drawing arrows and otherwise marking an image. Note that these tools are for annotation only. Annotations Tool Box 4.5.1...

ImageQuant 300 Capture User Manual 79 4.5.6 Text Background and Font The TEXT STYLE menu specifies the style of text. Click on the appropriate checkbox to show text with or without a background. An opaque background is useful if annotations will be made on an image that has wide variations in gray s...

ImageQuant 300 Capture User Manual 80 4.5.7 Text Angles In the TEXT ORIENT window, select whether text should be oriented vertically, horizontally, or at an angle (in 15 ° increments). Text Orient Selection Tools Note: only rotate fonts that are True Type (indicated by TT in front of the name); othe...

ImageQuant 300 Capture User Manual 81 The button labeled with a diagonal arrow draws arrows and straight lines. After clicking on the button, move the cursor to the position on the image where the line should begin. Press and hold the left mouse button. Using the mouse, move the cursor to the other ...

ImageQuant 300 Capture User Manual 82 Annotated image showing: freehand drawings, lines with various characteristics, circles, squares, text with various characteristics 4.5.9 The Editing Tools When the cursor is in “edit” mode, it can be clicked on an object to select it. (Note: the cursor can be t...

ImageQuant 300 Capture User Manual 85 4.6.3 Palettes 2 through 11 These are color substitution palettes in which the gray levels are translated into different color ranges. These palettes can be useful to help distinguish features and highlight details on an image. Palette 2 maps the gray scale leve...

ImageQuant 300 Capture User Manual 86 4.7 Image Filters ImageQuant Capture includes a variety of enhancement filters that can improve the appearance of an image. Some filters sharpen details, others smooth and reduce random noise. Still others help visualize edges and separate closely spaced bands o...

ImageQuant 300 Capture User Manual 87 4.7.1 General Information on How Filters Work To enhance an image, filters change the value assigned to each pixel. The new value assigned to a pixel is determined based on the values of the other pixels in its local vicinity (or “neighborhood”). The neighborhoo...

ImageQuant 300 Capture User Manual 88 4.7.2 Sharpening Filters These filters can increase image sharpness and provide edge enhancement. However image noise may be enhanced as well. These filters accentuate the high-frequency details of an image while leaving the low-frequency content intact. High fr...

ImageQuant 300 Capture User Manual 89 In addition, 3-D allows the user to control the ‘direction’ of the 3-D shadowing effect. Once the level is refined, just click on a direction arrow to visualize the shadowing adjustment. The default shadow direction is in the lower right hand corner direction an...

ImageQuant 300 Capture User Manual 90 which is an area with large changes in intensity, the subtraction yields light-colored pixel values. The larger the difference in intensities, the lighter the resultant pixels. Note: After applying the horizontal edge filter the entire image may appear black, an...

ImageQuant 300 Capture User Manual 94 Once all images have been captured, the Tool Box will automatically display the FrameGrabber tab. The buttons on this tab perform the following tasks: REC Opens the Camera Setup and Preview window to record a sequence. PLAY Display a continuous loop of all of th...

ImageQuant 300 Capture User Manual 95 4.9 FrameGrabber: Save/Load FrameGrabber Setup Routines Two buttons “Save Setup” and “Load Setup” allow you to save and load all FrameGrabber setup parameters. Files are saved as *.mvf files. 4.9.1 Frame Stacking At the top of the FrameGrabber setup window is an...

ImageQuant 300 Capture User Manual 96 4.9.2 Capturing a Color Image Using the FrameGrabber Function A color image can be generated by acquiring three images each taken with a red, green and blue emission filter. Once saved, these images are then combined in the Overlay pull-down menu. Open the image...

ImageQuant 300 Capture User Manual 97 Chapter 5: Analyzing Images Once you have acquired an image using ImageQuant Capture, you can transfer it to one of ImageQuant TL’s analysis modules using the toolbar options. The ImageQuant Capture toolbar contains 4 buttons: 1D, Array, Colony and Toolbox: 1D T...

ImageQuant 300 Capture User Manual 98 Appendix A: Opening ImageQuant Capture Files in Other Software Programs Files generated by ImageQuant have been tested in the software packages below. For successful imports, the command line is given. Programs for the Macintosh ® operating system were tested on...

ImageQuant 300 Capture User Manual 99 Using ResEdit 1. Save image in IS-1000/500 as a TIFF or MACTIFF 2. Obtain a copy of the freeware ResEdit by downloading from Apple Computers through the Internet. 3. Open ResEdit. An animated startup display will show up and continue until you click on the mouse...

ImageQuant 300 Capture User Manual 100 Appendix B: Security Features This feature should only be used by the purchaser or supervisor of the system. Remove this page from the manual and store it in a safe place. When the system is shared by a number of users, security features may be useful for regul...

ImageQuant 300 Capture User Manual 101 NOTE: You must exit and re-launch ImageQuant Capture in order for any changes to take place. If you decide not to edit the security settings at this time, simply click on the EXIT button to dismiss the dialog box. Setting Various Security Levels A variety of se...

ImageQuant 300 Capture User Manual 102 Each time a new name is used, it is added to the master user list. Since this list requires a password (in case the system is later changed to a closed, password protected system), ImageQuant will automatically assign the user’s name (in uppercase) as the passw...

ImageQuant 300 Capture User Manual 103 Option 4: Closed System with User Name Log In and Out A user can log in only by entering an authorized name. A user log is generated. In closed systems, the system supervisor may want to add or remove names from a list of authorized users. To do this, click the...