Agilent G1978B - Manuals

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Manual Agilent G1978B

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Page 2 - Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide; Notices; C A U T I O N; CAUTION; W A R N I N G

2 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide Notices © Agilent Technologies, Inc. 2008 No part of this manual may be reproduced in any form or by any means (including elec-tronic storage and retrieval or translation into a foreign language) without prior agree-ment and written c...

Page 3 - In This Guide; Installation; Verification; Methods

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 3 In This Guide This guide explains how to install, maintain and troubleshoot your nanoelectospray ion source. 1 Installation This chapter tells you how to install the multimode ion source. 2 Verification This chapter tells you how to i...

Page 5 - Content

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 5 Content 1 Installation 7 Installation 8 Step 1. Prepare to install 8 Step 2. Install the HV control PCA and cables 9 Changing Sources 13 To remove the multimode source 13 To convert from multimode to ESI, APCI or APPI 14 To convert fr...

Page 7 - Agilent Technologies

7 Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MSSet-Up Guide Agilent Technologies 1Installation Installation 8 Step 1. Prepare to install 8 Step 2. Install the HV control PCA and cables 9 Changing Sources 13 To remove the multimode source 13 To convert from multimode to ESI, APCI ...

Page 8 - Step 1. Prepare to install; Figure 1

8 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation Installation Installation This section explains how to install the G1978B multimode source on a 6100 Series Single Quad LC/MS instrument. The G1978A multimode source is not supported on these instruments. The 6100 Serie...

Page 9 - Step 2. Install the HV control PCA and cables; Figure 3

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 9 Installation 1 Step 2. Install the HV control PCA and cables • Valve BD-APCI supply, p/nG1960-60802 • Valve BD-APCI Needle Interlock, p/n G1960-60856 Figure 2 From left to right: G1946-80058, G1960-60802 and G1960-60856 Step 2. Instal...

Page 10 - Figure 5

10 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation Step 2. Install the HV control PCA and cables Figure 4 Connecting the valve PCA to the multimode HV power supply. 6 Install the APCI HV power supply. The APCI HV power supply is located at the end of the AUX Module. 7 ...

Page 11 - Figure 7; Figure 8

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 11 Installation 1 Step 2. Install the HV control PCA and cables Figure 6 Connecting the Vcap and Vchamber cables to the power supply. 9 Connect the long ribbon cable, p/n G1960-60802, from the APCI HV power supply to the valve PCA. Figu...

Page 12 - Method and Run Control View > MSD > Spray Chamber

12 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation Step 2. Install the HV control PCA and cables Figure 9 Connecting the HV PCA to the chassis. 12 Close the AUX Module cover and reconnect all cables. 13 Install the multimode source to the system and connect all connect...

Page 13 - Changing Sources; To remove the multimode source

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 13 Installation 1 Changing Sources Changing Sources To remove the multimode source Do the following steps to remove the multimode source. 1 Set the source temperatures for vaporizer heater and drying gas heater to minimum values to cool...

Page 14 - To convert from multimode to ESI, APCI or APPI

14 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation To convert from multimode to ESI, APCI or APPI 10 Place the source shipping cover on the source. To convert from multimode to ESI, APCI or APPI 1 If the source to be installed is an APPI source, disconnect the multimod...

Page 15 - To convert from ESI, APCI or APPI to the multimode source; MSD Tune; “Installation”

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 15 Installation 1 To convert from ESI, APCI or APPI to the multimode source To convert from ESI, APCI or APPI to the multimode source 1 Switch to the MSD Tune view. 2 Select Instrument/Set Spray Chamber and set all gas f lows and temper...

Page 17 - N O T E

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 17 Installation 1 To convert from ESI, APCI or APPI to the multimode source Figure 13 Multimode spray shield 13 Screw the multimode spray shield into the holder for the spray shield. See Figure 14 . Figure 14 Multimode spray shield inst...

Page 22 - Preparation; Table 1

22 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes Preparation 1 Transfer 1 mL of 5 ng/µL reserpine (Agilent G2423A) to...

Page 23 - Sensitivity Verification for Multimode in ESI Positive SIM Mode; Method and Run Control

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 23 Verification 2 To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes Sensitivity Verification for Multimode in ESI Positive SIM Mode 1 Start up the ChemStation software. 2 Change the view to MSD Tune , and start ...

Page 24 - Table 2

24 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes When the method is finished, a report prints showing the signal-to-noise ratio for the five blank and five sample peaks, and a blank-...

Page 25 - Sensitivity Verification for Multimode in ESI, Positive SIM Mode

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 25 Verification 2 To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes Sensitivity Verification for Multimode in ESI, Positive SIM Mode 1 Start up the ChemStation software. 2 Change the view to MSD Tune ,...

Page 27 - Table 3

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 27 Verification 2 To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes Preparation 1 Transfer 1 mL of 5 ng/µL reserpi...

Page 32 - To do an autotune; MSD Tune view

32 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To do an autotune To do an autotune Tuning the multimode source is done in MM-APCI mode only. Autotune is done from the same menu as with all sources. • From the MSD Tune view , select Instrument > Autotune menu ite...

Page 33 - Example of multimode verification report

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 33 Verification 2 Example of multimode verification report Example of multimode verification report

Page 37 - To create a method for positive/negative mixed mode operation

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 37 Methods 3 To create a method for positive/negative mixed mode operation To create a method for positive/negative mixed mode operation 1 Open the MSD Spray Chamber dialog box by clicking the Instrument > Set Up MSD Signals in the M...

Page 39 - To create a method for alternating ESI and APCI operation

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 39 Methods 3 To create a method for alternating ESI and APCI operation To create a method for alternating ESI and APCI operation 1 Open The MSD Spray Chamber dialog box by clicking Instrument > MSD Spray Chamber in the Method and Run...

Page 40 - OK

40 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 3 Methods To create a method for alternating ESI and APCI operation 9 Click the OK button. Figure 23 MM-ES and MM-APCI switching using Set Up MSD Signals dialog box N O T E In general, use the mixed mode operation (MM-ES+APCI setting...

Page 41 - Index

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 41 Index Index A autotune , 32 C converting from ESI, APCI or APPI , 15 converting to ESI or APCI , 14 D diverter valve inlet filter , 20 E ESI convert from , 15 convert to , 14 I installation , 7 , 21 L LC/MSD sample tubing , 20 M meth...