Beckman Coulter Allegra X-30 Series - User Manual

PN B05529AF v Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate the instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. I...

PN B05529AF vi Safety Notice Electrical Safety Electrical Safety To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect this equipment to earth-ground. To preserve this safety feature: • Make sure that the matching wall outlet receptacle is prope...

PN B05529AF vii Safety Notice Chemical and Biological Safety • Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running. During operation you should come within the envelope only to adjust instrument controls, if necessary. Never bring any flammable substances within th...

PN B05529AF viii Safety Notice Chemical and Biological Safety

ix Contents Revision History , iii Safety Notice , v Alerts for Danger, Warning, Caution, Important, and Note , v Safety During Installation and/or Maintenance , v Electrical Safety , vi Safety Against Risk of Fire , vi Mechanical Safety , vi Chemical and Biological Safety , vii Introduction , xv In...

x Contents Name Rating Plate , 1-8 Nonrefrigerated Model Specifications , 1-8 Refrigerated Model Specifications , 1-9 Available Rotors , 1-10 CHAPTER 2: Operation , 2-1 Introduction , 2-1 Run Procedure , 2-2 Preparation and Loading , 2-2 Entering Run Parameters , 2-5 Starting a Run , 2-9 Pulse Funct...

xi Contents APPENDIX A: Installation , A-1 Introduction , A-1 Installing the Instrument , A-1 Transportation Safety Devices , A-2 Electrical Requirements , A-2 Test Run , A-4 Beckman Coulter, Inc. Allegra X-30 Series Centrifuge Warranty

xii Illustrations Illustrations 1.1 Interior View of the Rotor Chamber , 1-3 1.2 The Power Switch , 1-4 1.3 System Panels , 1-4 1.4 System Keys , 1-5 1.5 Program Keys , 1-6 1.6 Digital Displays , 1-7 2.1 Tapered Sleeve Position , 2-2 2.2 Default Parameters , 2-6 3.1 Screw Locations , 3-4 3.2 Interna...

xiii Tables Tables 2.1 Acceleration/Deceleration Times (in minutes:seconds) , 2-9 3.1 Error Message Chart , 3-2 3.2 Troubleshooting Chart , 3-3

PN B05529AF xv Introduction Intended Use For In Vitro Diagnostic Use. The Allegra X-30 Series centrifuges are intended to separate human samples, including blood, urine, and other bodily fluids to prepare samples for downstream in vitro diagnostic procedures, which may include molecular diagnostic, ...

PN B05529AF xvi Introduction Conventions • APPENDIX A, Installation contains instructions for installing and connecting the centrifuge. NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and performance of this equipment could be impaired. Further, the use of ...

PN B05529AF xvii Introduction CE Mark It is very important that customers understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for deta...

PN B05529AF 1-1 CHAPTER 1 Description Introduction This chapter provides a brief physical and functional description of the Beckman Coulter Allegra X-30 Series centrifuges. The operating controls and indicators are also described; instructions for their use are in CHAPTER 2, Operation . Chemical com...

PN B05529AF 1-2 Description Chassis Safety Features Allegra X-30 Series centrifuges have been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft). Instrument safety features include: • The door has an electromechanical door-locking mechanism to prevent operator contact ...

PN B05529AF 1-3 Description Temperature Sensing and Control (refrigerated models only) 1 Figure 1.1 Interior View of the Rotor Chamber Drive The asynchronous three-phase direct-drive motor is brushless for clean, quiet operation. A tie-down screw is used to attach the rotor to the drive shaft. The r...

PN B05529AF 1-4 Description Controls and Indicators Controls and Indicators Power Switch The power switch is located on the centrifuge right side panel (see Figure 1.2 ). This two-position rocker switch ( I , on; O , off) controls electrical power to the centrifuge. Figure 1.2 The Power Switch NOTE ...

PN B05529AF 1-5 Description Controls and Indicators 1 System Keys The centrifuge operation is controlled through the system keys (see Figure 1.4 ). Each key (except the PULSE key) has an LED in the upper left corner that lights to indicate that the key can be activated. Figure 1.4 System Keys Progra...

PN B05529AF 1-7 Description Controls and Indicators 1 Digital Displays Digital displays indicate rotor speed, run time, rotor chamber temperature, and numbers that represent selected acceleration and deceleration profiles (see Figure 1.6 ). When the power is turned on, they show the operating parame...

PN B05529AF 1-8 Description Name Rating Plate Name Rating Plate The name rating plate is affixed to the rear of the centrifuge. Check that the line voltage agrees with the voltage listed on this name rating plate before connecting the centrifuge. Always mention the serial number and the model number...

PN B05529AF 1-9 Description Refrigerated Model Specifications 1 Refrigerated Model Specifications Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Noise level 0.91 m (3 ft) in front of instrument (approx.) £68 dBa Installat...

PN B05529AF 1-10 Description Available Rotors Available Rotors See the applicable rotor manual for information on rotor use, care and maintenance, and rotor accessories. Electrical supply Class I Maximum heat dissipation into room under steady - state conditions 3311 Btu/h (0.97 kW) Noise level 0.91...

PN B05529AF 2-1 CHAPTER 2 Operation Introduction This section contains operating procedures for the centrifuge, using any of the Beckman Coulter rotors designed for use in these centrifuges. Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation. To prevent c...

PN B05529AF 2-2 Operation Run Procedure Run Procedure The following detailed operating procedures are summarized at the end of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps. Preparation and Loading For fast temperat...

PN B05529AF 2-3 Operation Run Procedure 2 CAUTION Do not drop the rotor onto the drive shaft. The shaft can be damaged if the rotor is forced sideways or dropped onto it. Install the rotor by centering it over the shaft and carefully lowering it straight down. 6 Install the rotor according to the in...

PN B05529AF 2-4 Operation Run Procedure c. Seat the filled buckets onto the yoke pins. • Be sure to fill all positions on the yoke with buckets. For a fixed angle rotor, the steps include the following: a. Lower the rotor straight down onto the drive shaft. b. Tighten the tie-down screw clockwise on...

PN B05529AF 2-5 Operation Run Procedure 2 c. Attach the lid and tighten it. Some rotors have a knob and others have a bolt for the T-handle wrench. NOTE Fixed-angle rotors can be centrifuged without lids attached when longer tubes are used. CAUTION Always run any type of rotor with a balanced load. ...

PN B05529AF 2-6 Operation Run Procedure Figure 2.2 Default Parameters Selecting a Rotor Number Each rotor that can be run in the centrifuge has a rotor number; the number is engraved on the rotor (and on the lid of fixed angle rotors). The centrifuge internal memory contains a list of all rotors tha...

PN B05529AF 2-7 Operation Run Procedure 2 Setting RPM 1 Press the RPM key. • The last digit on the SPEED display ( 0 ) flashes, indicating that the RPM can be entered (in 100-RPM increments) with the cursor keys. 2 Press the ▲ or ▼ cursor key until the required RPM is displayed. • The corresponding ...

PN B05529AF 2-8 Operation Run Procedure The last digit on the TIME display flashes, indicating that the time can be entered with the cursor keys. 2 Press the ▲ or ▼ cursor key until the required run duration is displayed. Setting Run Temperature (refrigerated models only) Run temperature can be set ...

PN B05529AF 2-9 Operation Run Procedure 2 Setting Deceleration Rate The DECEL key is used to select deceleration rates that maintain optimum separation. When the DECEL key is pressed, the ACC/DEC display flashes, indicating that one of the ten preset deceleration rates can be entered with the cursor...

PN B05529AF 2-10 Operation Run Procedure 1 Check that all parameters are correct and the door is shut and latched. 2 Press the ENTER key, then the START key. • As the run begins, the instrument rotor identification system compares the rotor in the chamber to the entered rotor number and the speed en...

PN B05529AF 2-11 Operation Run Procedure 2 • If the PULSE key is pressed while the rotor is running at set speed, the rotor continues running at speed until the PULSE key is released. When the PULSE key is released, the rotor begins decelerating to 0rpm using maximum deceleration. • The centrifuge m...

PN B05529AF 2-12 Operation Unloading Press and hold the STOP key for deceleration at the maximum rate (see Table 2.1 ). NOTE If you hold the STOP key for more than a full second, the deceleration process cannot be interrupted; the centrifuge cannot be restarted until the rotor comes to a complete st...

PN B05529AF 2-13 Operation Summary of Run Procedures 2 Summary of Run Procedures For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. For low-temperature runs, precool the centrifuge by running a 30-minute cycle (with a precooled rotor installed) ...

PN B05529AF 2-14 Operation Summary of Run Procedures 8 After the rotor stops spinning and the OPEN DOOR light comes on, press the OPEN DOOR key to release the door latch; open the door. 9 Unload the rotor according to instructions in the applicable rotor manual. CAUTION If disassembly reveals eviden...

PN B05529AF 3-1 CHAPTER 3 Troubleshooting Introduction This section lists possible malfunctions, together with probable causes and corrective actions required. Maintenance procedures are contained in CHAPTER 4, Care and Maintenance . For any problems not covered here contact Beckman Coulter Field Se...

PN B05529AF 3-3 Troubleshooting Other Possible Problems 3 Other Possible Problems Possible malfunctions that may not be indicated by diagnostic messages are described in Table 3.2 , along with probable causes and corrective actions required. Possible causes for each problem are listed in the probabl...

PN B05529AF 3-4 Troubleshooting Emergency Access Emergency Access If the facility power fails only momentarily, the centrifuge resumes operation when power is restored and the rotor returns to set speed. However, if the rotor comes to a complete stop you have to restart the run when the power is res...

PN B05529AF 3-5 Troubleshooting Emergency Access 3 4 Set the covers aside. 5 Insert a Phillips-head screwdriver through one of the exposed holes in the centrifuge front panel and loosen, but do not remove, the screw. a. Repeat for the other screw. 6 Move the centrifuge forward on the bench until the...

PN B05529AF 3-6 Troubleshooting Emergency Access DANGER Never try to slow or stop the rotor by hand. 11 After removing the rotor, replace the front panel, screws, and covers.

PN B05529AF 4-1 CHAPTER 4 Care and Maintenance Introduction For maintenance not covered in this manual, contact Beckman Coulter Field Service. User messages are discussed in CHAPTER 3, Troubleshooting . Refer to the applicable rotor manual and to Chemical Resistances (publication IN-175) for instruc...

PN B05529AF 4-2 Care and Maintenance Cleaning 2 Regularly check the air intake and exhaust vents for obstructions. a. Keep vents clear and clean. 3 Use a sponge to wipe condensation out of the chamber bowl between runs to prevent chamber icing (refrigerated models). a. If chamber icing occurs, defro...

PN B05529AF 4-3 Care and Maintenance Tube Breakage 4 4 Clean the centrifuge case and door by wiping with a cloth dampened with diluted Solution 555. Do not use acetone or other solvents. Tube Breakage If a glass tube breaks, and all the glass is not contained in the bucket or rotor, you must thoroug...

PN B05529AF 4-4 Care and Maintenance Storage and Transport Storage and Transport Storage Before storing a centrifuge for an extended period, return it to its original shipping container to protect it from dust and dirt. Reinsert the shipping foam (removed at time of centrifuge installation) into the...

PN B05529AF A-1 APPENDIX A Installation Introduction This chapter contains instructions for installing and connecting the centrifuge. Check that required clearances and electrical power are available. WARNING These centrifuges each weigh 48 kg/106 lb (non-refrigerated model) or 78 kg/172 lb (refrige...

PN B05529AF A-2 Installation Transportation Safety Devices a. Make sure that the centrifuge front feet are fully supported on the table. b. Locate the centrifuge in an area with sufficient ventilation to allow for heat dissipation. c. Check that there are 7.6-cm (3-in.) clearances at the sides of th...

PN B05529AF A-4 Installation Test Run WARNING To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. To preserve this safety feature: • Make sure that the matching wall outlet receptacle is properly wired and earth...

© 2022 Beckman Coulter, Inc. All Rights Reserved www.beckman.com Related Documents Chemical Resistances for Beckman Coulter Centrifugation Products PN IN-175 Available in hard copy or electronic pdf by request. Available at www.beckman.com