Page 2 - Installation and Operation Guide; Notices; Manual Part Number; Warranty; separate agreement shall control.; Safety Notices; C A U T I O N; CAUTION; W A R N I N G; Acknowledgement
2 Installation and Operation Guide Notices ©Agilent Technologies, Inc. 2006 No part of this manual may be reproduced in any form or by any means (including elec-tronic storage and retrieval or translation into a foreign language) without prior agree-ment and written consent from Agilent Technologies...
Page 3 - Introduction
Installation and Operation Guide 3 In this Guide. . . This Installation and Operation Guide contains information for installing and using an eff luent splitter on an Agilent 6890 gas chromatograph (GC). The G3180 splitter is intended for use with capillary columns and uses makeup gas to maintain ade...
Page 5 - Contents
Installation and Operation Guide 5 Contents 1 Introduction Overview 8 How It Works 9 Details 10 Metal ferrules 10 Microfluidic plate 10 Constant pressure operation 10 Calculation of chromatographic parameters 11 GC Requirements 12 Other Requirements 12 Parts Supplied 13 Part Identification 14 Parts ...
Page 6 - Splitter Configurations
6 Installation and Operation Guide 3 Splitter Configurations Typical Configurations 26 Splitting to an MSD 28 Custom Configurations 29 Restrictor id and length 32 Maximum and minimum flows 33 Column outlet pressure 34 Inlet pressure 34 Restrictor and Column Installation 35 Install the column 35 Conn...
Page 7 - Agilent Technologies
7 Agilent G3180B Splitter KitInstallation and Operation Guide Agilent Technologies 1Introduction Overview 8 How It Works 9 Details 10 Metal ferrules 10 Microfluidic plate 10 Constant pressure operation 10 Calculation of chromatographic parameters 11 GC Requirements 12 Other Requirements 12 Parts Sup...
Page 8 - Overview
8 Installation and Operation Guide 1 Introduction Overview Splitter installation is done in three steps: 1 Hardware installation. This gets the hardware installed and the gas f lows connected. 2 Restrictor configuration. You can choose to use a typical, precalculated configuration or create a custom...
Page 9 - How It Works; • Atmospheric pressure; Figure 1
Introduction 1 Installation and Operation Guide 9 How It Works The splitter divides the eff luent from a column between two different detectors. The detectors can be operating at different pressures, that is, any mix of the following can be used: • Atmospheric pressure FID (f lame ionization detecto...
Page 10 - Details; Metal ferrules
10 Installation and Operation Guide 1 Introduction The column f low mixes with the makeup f low in the splitter. This mixture then f lows through lengths of uncoated, deactivated, fused-silica tubing to each detector. These tubes act as f low restrictors. While the f low through each restrictor chan...
Page 11 - Calculation of chromatographic parameters
Introduction 1 Installation and Operation Guide 11 Calculation of chromatographic parameters Because the pressure at the split point is known and constant, the chromatographic parameters can be calculated before setup. This is especially useful with GC/MSD setups, where there are limitations on the ...
Page 12 - GC Requirements; Other Requirements
12 Installation and Operation Guide 1 Introduction GC Requirements The splitter mounts in an Agilent 6890 series GC. The splitter requires an electronically controlled pressure source such as the Three Channel Pressure controller (6890 option 205, 301, or 308) or a Pneumatics Control Module (PCM). O...
Page 13 - Parts Supplied; Table 1
Introduction 1 Installation and Operation Guide 13 Parts Supplied The G3180B kit contains the following parts ( Table 1 ). Table 1 Parts supplied Part number Description Quantity 0100-0124 Union, stainless steel, 1/16-inch tubing 2 0100-0241 Union, stainless steel, 1/8 to 1/16-inch reducing 1 G1580-...
Page 14 - Part Identification; Figure 2
14 Installation and Operation Guide 1 Introduction Part Identification Most of the kit parts are easily recognized. The unique ones are identified in Figure 2 . Figure 2 Part identification Capillary columnspring clips Valve box Oven bracket Compact splitter blanking plate assembly with makeup gasas...
Page 15 - Parts Not Supplied
Introduction 1 Installation and Operation Guide 15 Parts Not Supplied Brown-dot frit (19231-60610) Tools Required Side cutter, large Open-end wrenches
Page 18 - Figure 3
18 Installation and Operation Guide 2 Hardware Installation Prepare the GC 1 Raise the GC top cover to expose the oven top. 2 Remove the valve box cutout using a side cutter ( Figure 3 ). W A R N I N G Turn the power off and disconnect the power cord before proceeding. Figure 3 Remove the valve box ...
Page 19 - Figure 4; Figure 5
Hardware Installation 2 Installation and Operation Guide 19 3 This exposes a layer of soft insulation. Remove it to expose the hard oven insulation. Remove the precut insulation piece at the location shown in Figure 4 . 4 Replace the soft insulation. Install the valve box blanking plate, using one s...
Page 20 - Install the Column Clips; Figure 6
20 Installation and Operation Guide 2 Hardware Installation Install the Column Clips Install the four column clips on the oven shroud ( Figure 6 ). Figure 6 Oven clips Ovenshroud Clip(1 of 4)
Page 21 - Install the Bracket and Splitter; N O T E; Figure 7
Hardware Installation 2 Installation and Operation Guide 21 Install the Bracket and Splitter The splitter is usually installed on the right side of the oven. 1 Place the bracket against the side of the oven. The two notches should be up and the standoffs should face the center of the oven. 2 Use two...
Page 22 - Figure 8
22 Installation and Operation Guide 2 Hardware Installation 3 Open the plastic bag and remove the splitter assembly. Install a plastic cap on the end of the makeup gas tubing. Place small pieces of tape over the open end of the fittings. 4 Prebend the tubing according to Figure 8 . This will make sp...
Page 23 - Figure 9
Hardware Installation 2 Installation and Operation Guide 23 6 Route the prebent tubing against the oven wall and top to keep it clean for future maintenance. It should be behind the back detector location. 7 Screw the splitter assembly to the bracket (three screws). See Figure 9 . Figure 9 Installin...
Page 24 - Connect the Makeup Gas Supply; To supply the makeup gas froma PCM
24 Installation and Operation Guide 2 Hardware Installation Connect the Makeup Gas Supply Connect the makeup gas source to the PCM or Auxiliary Pressure controller. To supply the makeup gas froma PCM 1 Connect the tubing from the PCM to the 1 meter length of stainless steel tubing from the kit with ...
Page 26 - Typical Configurations
26 Installation and Operation Guide 3 Splitter Configurations Typical Configurations The important parameters when setting up a splitter are the lengths and diameters of the restrictor tubes that go to the two detectors. The dimensions of the restrictors are chosen to give the desired split ratio, f...
Page 27 - Configuration 1; Table 3
Splitter Configurations 3 Installation and Operation Guide 27 To use the tables, select the configuration you wish to set up. For example, Configuration 1 splits column eff luent equally between two atmospheric pressure detectors (FID, TCD, ECD, FPD, and NPD). To plumb this system, 0.544-m lengths o...
Page 28 - Splitting to an MSD
28 Installation and Operation Guide 3 Splitter Configurations For constant pressure methods, first find the maximum f low as above. Use the GC, ChemStation, Flow Calculator Software or the Method Translation Software to find the inlet pressure that gives the maximum f low at the upper temperature of...
Page 29 - Custom Configurations; Effluent Splitter Calculator (with Makeup)
Splitter Configurations 3 Installation and Operation Guide 29 Custom Configurations The CD supplied with this kit contains three software tools: Effluent Splitter Calculator (with Makeup) Calculates dimensions (length and inside diameter) of restrictors to obtain a desired split ratio ( Figure 11 )....
Page 30 - GC Method Translation
30 Installation and Operation Guide 3 Splitter Configurations GC Method Translation Converts an analytical method from one set of operating conditions to another ( Figure 12 ). Figure 12 GC Method Translation
Page 31 - Column Pressure/Flow Calculator; Start
Splitter Configurations 3 Installation and Operation Guide 31 Column Pressure/Flow Calculator Calculates f lows and pressures for a given set of column (or restrictor) dimensions ( Figure 13 ). These tools allow you to perform all the calculations needed to create a custom splitter configuration. We...
Page 32 - Restrictor id and length; Column f low; Carrier gas type; Flow Ratio of Detector 2 to Detector 1
32 Installation and Operation Guide 3 Splitter Configurations Restrictor id and length 1 Run the Eff luent Splitter Calculator and enter the following information. The calculator provides a list of possible restrictors. • Column f low . Use the ChemStation, GC, Flow Calculator, or Method Translation...
Page 33 - Maximum and minimum flows; Table 4
Splitter Configurations 3 Installation and Operation Guide 33 Maximum and minimum flows The maximum suggested f low for MSDs depends on the vacuum pump used. For diffusion pump and standard turbo systems, the f low should not exceed 2 mL/min. For performance turbo systems, the f low should not excee...
Page 34 - Column outlet pressure
34 Installation and Operation Guide 3 Splitter Configurations Column outlet pressure The 6890 GC needs to know the pressure at the end of the column to be able to calculate column f lows. Use either the GC keyboard or the ChemStation to set the outlet pressure for the column to 3.8 psig. The ChemSta...
Page 35 - Restrictor and Column Installation; Install the column; Connect the splitter
Splitter Configurations 3 Installation and Operation Guide 35 Restrictor and Column Installation Install the column 1 Hang the analytical column on the column clips. The clips hold the outside of the wire “basket” that supports the column. Adjust the clips if necessary. 2 Connect the column to the i...
Page 36 - Do not overtighten the fittings. The dashed line in
36 Installation and Operation Guide 3 Splitter Configurations Figure 15 Restrictor and column connections Column exit Restrictor 1to Detector 1 Restrictor 2to Detector 2 To makeupgas supply C A U T I O N Do not overtighten the fittings. The dashed line in Figure 16 (about 15° clockwise from finger-t...
Page 37 - Disconnect tubing from the splitter
Splitter Configurations 3 Installation and Operation Guide 37 Disconnect tubing from the splitter Loosen and remove the internal nut from the splitter fitting. Usually the tubing and ferrule will fall out of the fitting. Occasionally the ferrule will stick in the fitting. If this happens, use a poin...
Page 38 - Protect the column and restrictors
38 Installation and Operation Guide 3 Splitter Configurations Protect the column and restrictors Column and restrictor tubes with swaged metal ferrules can be disconnected and reconnected several times. To protect the tubing end, use one of the brass-sealing caps from the kit. Tighten to finger-tigh...
Page 40 - An Example; Column flow; None
40 Installation and Operation Guide 4 Operation An Example Assume we have a method that uses an HP-5MS column (30 m × 250 µm id × 0.25-µm film thickness) to measure pesticides with an MSD. The initial oven temperature is 70 °C and is programmed to 280 ο C. The method is run in constant pressure mode...
Page 42 - Select restrictors
42 Installation and Operation Guide 4 Operation Select restrictors Start up the spreadsheet "splitter_calc.xls" in Excel. We will choose to have 2 mL/min go to the ECD initially. With a split ratio of 3, this will send 6 mL/min to the MSD. This f low is acceptable with a performance turbo sy...
Page 43 - Calculate column flow
Operation 4 Installation and Operation Guide 43 The calculator lists the lengths required for the different sizes of uncoated, deactivated, fused-silica, restrictor tubing available. Choose the id tubing that gives the shortest length of at least 0.3 m for most detectors and 0.8 m for MSDs. In this ...
Page 44 - Calculate ECD restrictor flow
44 Installation and Operation Guide 4 Operation Calculate ECD restrictor flow The f low through the ECD restrictor at 280 °C is calculated to be 0.88 mL/min ( Figure 21 ). This f low is higher than the minimum 0.72 mL/min suggested for helium in 0.18-mm id tubing. Figure 21 ECD restrictor flow calcu...
Page 45 - Calculate MSD restrictor flow
Operation 4 Installation and Operation Guide 45 Calculate MSD restrictor flow The f low through the MSD restrictor ( Figure 22 ) at 280 °C is: The f low to the MSD at 280 °C is 2.65 mL/min. This f low is higher than the minimum 0.72 mL/min suggested for helium in 0.18-mm id tubing. The calculated ma...
Page 46 - Changing Columns Without Venting the MSD
46 Installation and Operation Guide 4 Operation Changing Columns Without Venting the MSD For systems that use an MSD attached to the splitter, one added advantage is the GC column can be changed without venting the MSD. When the column is disconnected from the splitter plate, the makeup gas purges a...
Page 47 - Backflushing the Column
Operation 4 Installation and Operation Guide 47 Backflushing the Column One useful feature available with EPC control of the makeup is the ability to backf lush unwanted higher boiling analytes from the column. Use of this feature requires that the split/splitless inlet be used. Backf lushing reduce...
Page 49 - Index
Installation and Operation Guide 49 Index A Absolute pressure , 41 Aux EPC , 27 Auxiliary Pressure controller , 24 B Backflushing , 47 C Changing columns , 46 Column connections , 35 flow , 10 , 26 , 27 , 43 outlet pressure , 34 Column clips , 35 Column effluent , 27 Configuration , 9 custom , 25 , ...
